Labshake search
Citations for Millipore Sigma :
7151 - 7200 of 10000+ citations for P N Nonylphenol 13C6 99% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2024Quote: ... + final concentration of 10 mM HEPES Buffer (Fisher; BP299-100) + trypsin inhibitor soybean (1 mg/ml in EBSS at a final concentration of 0.2%; Sigma, T9253) + 60 µl of fetal bovine serum (Biowest ...
-
bioRxiv - Neuroscience 2024Quote: ... in 0.15 M KCl TRAP wash buffer (10 mM HEPES-KOH at pH 7.4, 5 mM MgCl2, 150 mM KCl, 1% NP-40, supplemented with 100 µg/mL cycloheximide [Millipore Sigma C7698-1g] in methanol ...
-
bioRxiv - Neuroscience 2024Quote: ... Samples were then diluted to 4 mL with DPBS and transferred to 100 K Amicon Ultra Centrifugal Filters (Sigma-Aldrich), followed by centrifugation at 5,000 × g for 30 min ...
-
bioRxiv - Cell Biology 2024Quote: ... CAS#96736-11-7) was used at a final concentration of 100 μg/ml and thiabendazole (TBZ) (Sigma-Aldrich; T8904) at 5-15 μg/ml in YE media where appropriate ...
-
bioRxiv - Cell Biology 2024Quote: A subline of glioma U251 cells17 and COS7 cells were seeded on glass-bottom dishes (Matsunami, #D11130H) coated with 100 μg/ml poly-D-lysine (Sigma) and 5 μg/ml laminin (Lonza) ...
-
bioRxiv - Biochemistry 2024Quote: ... The purified protein was concentrated to 20 mg/mL with an Amicon ultrafiltration device (100 kDa cutoff for NrdD and 10 kDa cutoff for NrdG; Millipore), frozen in liquid nitrogen ...
-
bioRxiv - Cell Biology 2024Quote: ... HEPES (20) with a pH of 7.2 with KOH and included 70-100 μg/mL of amphotericin B (Millipore-Sigma) added just prior to recording ...
-
bioRxiv - Microbiology 2024Quote: Bacterial strains used throughout the study were routinely plated on LB agar (Difco) plates supplemented with appropriate antibiotics (Ampicillin: 100 μg ml-1 (Sigma), Spectinomycin 100 μg ml-1 (Sigma) ...
-
bioRxiv - Bioengineering 2023Quote: ... substrates containing 400 nm diameter pores at a density of 2·106 pores·cm-2 were coated with 100 µL of 1 µg/mL human plasma fibronectin (Sigma-Aldrich) in PBS (Gibco ...
-
bioRxiv - Biochemistry 2023Quote: ... and resuspended in complete RMPI with 1% Penicillin/streptomycin (100 units penicillin and 0.1 mg/ml streptomycin) (Penicillin-Streptomycin 100x, Cat#. P4333, Sigma-Aldrich). Subsequently,1.6x105 parasites/well (MOI ...
-
bioRxiv - Bioengineering 2024Quote: ... + final concentration of 10 mM HEPES Buffer (Fisher; BP299-100) + trypsin inhibitor soybean (1 mg/ml in EBSS at a final concentration of 0.2%; Sigma, T9253) + 60 µl of fetal bovine serum (Biowest ...
-
bioRxiv - Plant Biology 2022Quote: ... The quantification was conducted by means of standard curves (1, 2.5, 5, 10, 25, 50, 75, 100, 200 and 500 ng/mL of ABA, JA and SA, Sigma Aldrich).
-
bioRxiv - Molecular Biology 2023Quote: ... Thereupon the samples were transferred to 1.5 ml tubes containing 100 μl of Anti-FG M2 agarose beads (Millipore-Sigma) and incubated at 25° C for 2 hours ...
-
bioRxiv - Molecular Biology 2023Quote: Yp strains were induced to upregulate expression of the T3SS and associated effectors by taking 100 μL of stationary phase overnight cultures and adding to 3 mL 2x YT media containing 20mM sodium oxalate (Sigma) and 20mM MgCl (Sigma) ...
-
bioRxiv - Neuroscience 2023Quote: ... and immediately homogenised in ice-cold homogenisation buffer (50 mM Tris-HCl pH 7.5, 100 mM KCl, 12 mM MgCl2, 1 mg/ml Heparin [Sigma-Aldrich] ...
-
bioRxiv - Cancer Biology 2023Quote: ... fixed cells were pelleted and washed twice with PBS and resuspended in 300 μL hypotonic buffer (1 g/L sodium citrate buffer, 0.1% Triton-X 100) supplemented with 40 μg/mL propidium iodide (Sigma-Aldrich) and 100 μg/mL of RNase A (Thermo Fisher Scientific) ...
-
bioRxiv - Systems Biology 2023Quote: ... Slides were washed in containers containing large volumes and under agitation (400 rpm) as following: 100 mL of preheated buffer 1 (2X SSC and 0.1X SDS (Sigma 71736); 50°C ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein samples of the peak fractions were collected and concentrated to 6 mg/ml using a 100 kDa molecular weight cut-off concentrator (Millipore).
-
bioRxiv - Molecular Biology 2023Quote: ... coli MC1061 strain and Luria-Bertani (LB)-agar supplemented with ampicillin (100 μg/mL, Sigma-Aldrich, St. Louis, MO, USA) for selection ...
-
bioRxiv - Microbiology 2023Quote: ... or yeast casitone fatty acid broth (YCFA) with 4.4 mg/100 mL sterile filtered bilirubin dissolved in dimethylsulfoxide (DMSO, Sigma-Aldrich). The bacteria were grown anaerobically for 24 hours before starting the chloroform extraction.
-
bioRxiv - Microbiology 2022Quote: ... The beads were washed seven times with 1 ml IP buffer and eluted with 100 μl IP buffer supplemented with 6.67 mM of m6A salt (M2780, Sigma-Aldrich) at 4 °C for 1 h ...
-
bioRxiv - Neuroscience 2023Quote: ... Cell lysis was performed by dissolving the bacterial pellet in 100 mL of 40 mM Tris-hydrochloride (HCl; Sigma-Aldrich) buffer (pH 7.5 ...
-
PQS and Pyochelin in Pseudomonas aeruginosa Share Inner Membrane Transporters to Mediate Iron UptakebioRxiv - Microbiology 2023Quote: ... A total of 100 µL of 4 mg/mL 2- nitrophenyl β-D-galactopyranoside (ONPG) (Sigma, St. Louis, MO, USA) was added to the cells ...
-
bioRxiv - Synthetic Biology 2023Quote: ... were pipetted into a clean 1.5 mL glass vial and mixed with 25 µL of the Inside-solution (I-solution, 3.2 µg mL−1 ciprofloxacin and 100 µM dextran (50 kDa, Sigma-Aldrich) in PBM9 (glucose replaced with 0.333% w/v sucrose) ...
-
bioRxiv - Systems Biology 2023Quote: ... or to a volume of 20-30 mL (if using EVs for proteomics) in a 100 kDa MWCO ultrafiltration concentrator (Millipore). EVs were isolated from conditioned media by differential ultracentrifugation in a SW32Ti swinging bucket rotor as described above ...
-
bioRxiv - Cell Biology 2023Quote: ... and dounced for 100 strokes in a 40-ml Dounce homogeniser (using the small clearance pestle, or the pestle B; cat. D9188, Sigma), followed by two times of centrifugation for 5 min at 600g at 4 °C ...
-
bioRxiv - Plant Biology 2023Quote: Root tips of GFP fused protein transformed Arabidopsis were transferred onto slides and covered with a drop of 100 times diluted Propidium Iodide (PI) solution (1.0 mg/mL) (Sigma-Aldrich) for one minute ...
-
bioRxiv - Biophysics 2023Quote: ... Peak fractions were pooled using a 4 ml Amicon spin concentrator with a 100-kDa molecular weight cutoff membrane (Millipore) and used immediately for grid preparation or hydrolysis measurements.
-
bioRxiv - Biochemistry 2023Quote: ... Peak fractions were concentrated to 2 mg mL-1 using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore). To each 300-mesh holey carbon grid (Au R1.2/1.3 ...
-
bioRxiv - Genomics 2023Quote: ... the aqueous phase was passed through a 0.5-ml Amicon Ultra Centrifugal Filter with a 100 kDa cutoff (Millipore, UFC510024) by 3 min of centrifugation at 14,000g ...
-
bioRxiv - Microbiology 2023Quote: ... The resin was washed and incubated with 50 U of TEV protease and 100 µg/mL FLAG peptide (Sigma-aldrich) for 2 h at 18°C ...
-
bioRxiv - Plant Biology 2023Quote: ... water was removed from wells and replaced with 100 μL of an immune-eliciting solution containing 34 μg/mL luminol (Sigma), 20 μg/mL horseradish peroxidase (Sigma) ...
-
bioRxiv - Pathology 2023Quote: ... Nuclear fast red solution was prepared using 0.1 g nuclear fast red powder in 100 mL distilled water with 5 g Al2(SO4)3 (Sigma-Aldrich). Deparaffinized slides were washed with distilled water and then incubated with the Alcian blue solution for 30 minutes at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... washed in PBS/0.1 % Triton X-100 and treated with antigen retrieval solution [proteinase K 20 mg/ml (Sigma-Aldrich) dissolved in 10 mM Tris pH 8.0 ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... slides were washed for 5 min in 0.1x SSC with 1% Triton X-100 at 62°C and counterstained with 0.5 μg/ml DAPI (4’,6-diamidino-2-phenylindole; Sigma-Aldrich) in antifade with DABCO (1,4-diazabicyclo (2.2.2)-octane ...
-
bioRxiv - Biochemistry 2023Quote: 3×10 cm plates of cells were incubated 15 min at 37°C with fresh medium supplemented with 100 μg/mL of cycloheximide (Sigma). Where indicated cells were treated with 2 μg/mL of harringtonine or 100 μg/mL of puromycin ...
-
bioRxiv - Biophysics 2023Quote: ... and was subsequently concentrated to 1 mL using a 100 kDa cut-off Amicon ultra centrifugal filter (Merck Millipore, Germany). The concentrated protein sample was further purified by size exclusion chromatography (SEC ...
-
bioRxiv - Cancer Biology 2023Quote: ... fixed at room temperature for 10 min in PBS with 4% (v/v) formaldehyde and blocked with 100 μg/mL DNase-free RNase A (Sigma) in antibody dilution buffer (20 mM Tris-HCl ...
-
bioRxiv - Genomics 2022Quote: ... During sampling we set a flow rate of 200 l/minute for a total of 60 minutes with 10 ml 0.05 % (v/v) Triton X-100 (T8787-50ML, Merck Sigma Aldrich) as collection buffer prepared with UltraPure DNase/RNase free ...
-
bioRxiv - Systems Biology 2023Quote: ... and DNase treatment were carried out as described but with cell wall permeabilization using 100 µg/ml lysostaphin (Sigma-Aldrich) for S ...
-
bioRxiv - Microbiology 2023Quote: ... Protein was then eluted off the agarose by resuspension in 100 µL of 3X FLAG elution buffer (3 µL of 5 mg/mL Sigma 3X FLAG peptide solution + 97 µL of coIP buffer 2 ...
-
bioRxiv - Immunology 2023Quote: ... cells were washed twice in PBS and the pellet then treated with RNase A diluted in PBS (100 μg/ml, Sigma) before incubation with propidium iodide (50 μg/ml ...
-
Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium IndicatorsbioRxiv - Bioengineering 2023Quote: ... + final concentration of 10 mM HEPES Buffer (Fisher; BP299-100) + trypsin inhibitor soybean (1 mg/ml in EBSS at a final concentration of 0.2%; Sigma, T9253) + 60 µl of fetal bovine serum (Biowest ...
-
bioRxiv - Microbiology 2023Quote: ... A single colony with two plasmids was grown in liquid LB medium supplemented with 100 μg/mL ampicillin (Sigma-Aldrich) and 20 μg/mL chloramphenicol (AppliChem ...
-
bioRxiv - Biochemistry 2023Quote: ... and the protein fractions were collected and concentrated to 4 mg/mL by using a 100 kDa molecular weight cut-off concentrator (Millipore).
-
bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 1 mL fresh LDNT media with 100 μM reduced L-glutathione (Sigma-Aldrich, St. Louis, MO). 125 μL of cells containing 1.25×106 cells were plated in a Lab-Tek II chambered coverglass #1.5 Borosilicate chamber (Nunc International ...
-
bioRxiv - Biophysics 2023Quote: ... The eluate was then concentrated using a 15 ml Amicon spin concentrator with a 100-kDa molecular weight cutoff membrane (Millipore) and purified by size exclusion chromatography (SEC ...
-
bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted with 100 µL of elution buffer supplemented with 200 µg/mL 3× Flag-peptide (Sigma-Aldrich, #F4799) and stored at -80◦C until usage ...
-
bioRxiv - Immunology 2023Quote: ... The remaining colon tissue was minced using scissors in digestion media (RPMI, 100 μg/mL of DNASE I (Sigma Aldrich), 0.5 mg/mL Collagenase type IV (Worthington Biochemical Company) ...
-
bioRxiv - Biophysics 2023Quote: ... was concentrated to ∼10 ml total volume using a 100 kDa MWCO filter and incubated with anti-Flag M2 affinity resin (Sigma) in batch for 4 hrs ...