Labshake search
Citations for Millipore Sigma :
7151 - 7200 of 10000+ citations for 6 METHOXY PYRIDINE 3 SULFONYL CHLORIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... Debris was removed by 2 successive centrifugations (6 minutes at 600 rpm) and cells were plated onto coverslips coated with 0.01% poly-L-ornithine (Sigma, P4957) and incubated overnight at 37 °C with 5% CO2 in serum-free DMEM.
-
bioRxiv - Genomics 2023Quote: C57BL/6 mouse ESC were purchased from ATCC (SCRC1002) and maintained onto 1% gelatin-coated (Sigma-Aldrich, Cat. No G1393) dishes in DMEM knockout medium (Life Technologies ...
-
bioRxiv - Cancer Biology 2023Quote: ... Monocytes were then isolated with CD14+ microbeads (MACS Miltenyi) and incubated for 6 days in RPMI/10%FBS and 1% penicillin/streptomycin solution (Sigma) supplemented with 50 ng/mL human GM-CSF (R&D Systems ...
-
bioRxiv - Plant Biology 2023Quote: ... and 2 mL of acid ninhydrin (glacial acetic acid, 6 M phosphoric acid, ninhydrin (2,2-dihydroxyindane-1,3-dione, Sigma, ON, Canada) were boiled in a water bath at 100 °C for 1 hr ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with staining medium and re-suspended in staining media supplemented with 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from analyses ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with staining medium and re-suspended in 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from sorts and analyses ...
-
bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
-
bioRxiv - Molecular Biology 2023Quote: MDA-MB-231 cells were incubated with 50 ng/mL of ethidium bromide and passaged for ∼6 weeks in D10F supplemented with uridine (50 mg/mL, Sigma). Upon confirmation of loss of mitochondrial transcriptional activity by qPCR and Northern blotting ...
-
bioRxiv - Neuroscience 2023Quote: ... and the AMPA receptor inhibitor 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 µM, Sigma Aldrich, St Louis, MO, USA). This solution was applied to the autaptic culture neurons for 2 min ...
-
bioRxiv - Neuroscience 2023Quote: ... Induced neurons were replated onto 6-well tissue culture plates at 450k cells/well on pre-coated with poly-L-orthnithine (Sigma), 4 μg/ml Laminin (Roche Basel ...
-
bioRxiv - Microbiology 2023Quote: ... or CEM were infected overnight in a 6-well plate in the presence of 0.5 μg/mL polybrene (Sigma-Aldrich). The following day ...
-
bioRxiv - Cancer Biology 2023Quote: ... 6-week-old ElaK mice were first treated with tamoxifen (T5648-1G, Sigma Aldrich. 30 mg/ml in corn oil) by oral gavage and 4-hydroxytamoxifen (H7904-25mg ...
-
bioRxiv - Cancer Biology 2023Quote: ... TMT-labeled samples were incubated in IP buffer consisting of 1% Nonidet P-40 with protein G agarose beads conjugated to 24 μg of 4G10 V312 IgG and 6 μg of PT-66 (P3300, Sigma) overnight at 4℃ ...
-
bioRxiv - Cancer Biology 2023Quote: Cells were seeded in 6 cm dishes one day prior to treatment with 1 μg/ml actinomycin D (Sigma-Aldrich) for the indicated time ...
-
bioRxiv - Developmental Biology 2023Quote: ... Four- to five-week-old C57Bl/6 females were superovulated and MII oocytes collected in fertilisation media (Human Tubal Fluid (HTF, MR-070, Sigma) containing 4 mg/ml BSA and 25mM L-Glutathione 1 (GSH)) ...
-
bioRxiv - Bioengineering 2023Quote: ... The resulting submucosa was cut into 6 inch pieces and decellularized using 4% alcohol/0.1% peracetic acid (v/v, Sigma-Aldrich) and washed thoroughly with 1X PBS and Type 1 water followed by lyophilization and comminuted into an injectable particulate form via cryogenic milling and sieving through 425 µM pore sieve ...
-
bioRxiv - Biochemistry 2023Quote: ... Eluted and purified mGlu5-5M receptor and mGlu5-5M_W785A receptor mutant bound to quisqualate and PAM were concentrated to 5-6 mg/ml using Amicon Ultra-4 centrifugal concentrator 100 kDa (Millipore) for cryoEM grid preparation and analysis.
-
bioRxiv - Biophysics 2023Quote: ... 1.5 – 2 million cells were plated on a 6-well-plate coated with 10 µg/ml fibronectin (Sigma; F1141-5MG) and maintained in IMDM (GlutaMAX ...
-
bioRxiv - Cancer Biology 2023Quote: ... dissociated to single cell suspensions and cultured in RPMI-1640 (complete medium) supplemented with 60 μM 6-Thioguanine (Sigma-Aldrich) as previously described (18 ...
-
bioRxiv - Cancer Biology 2023Quote: Bone marrow cells were acquired by flushing the femurs and tibias of 6- to 8-week-old female BALB/c mice and treated with red blood cell lysis buffer (Sigma). Lineage negative hematopoietic stem and progenitor cells (HSPCs ...
-
bioRxiv - Biochemistry 2023Quote: ... Protein was eluted from the resin by applying 6 mL of wash buffer supplemented with 20 mM reduced glutathione (Sigma).
-
bioRxiv - Neuroscience 2023Quote: ... and plated at a density of 1-1.2x106 cells/ml onto 6-well plates treated with poly-D-lysine (EMD Millipore). Cells were cultured in Neurobasal A medium supplemented with 2% B27 ...
-
bioRxiv - Molecular Biology 2023Quote: The resulting oxidized DNA samples (∼300ng) were mixed with 20 mM phosphate buffer (pH 6) and 5.1 mM (+)-biotinamidohexanoic acid hydrazide (Sigma-Aldrich), and incubated at 40°C for 4 hours ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were incubated for 30 minutes on ice with 6 nm (Jackson#711-195-152) or 5 nm (Sigma#G7277) diameter immunogold coupled secondary antibodies in DMEM-FBS-HEPES ...
-
bioRxiv - Plant Biology 2024Quote: ... 6 ml of the suspension was equally distributed and loaded on top of four continuous Percoll (Sigma Aldrich, Heidelberg, Germany) gradients (containing 50% Percoll / 0.4 M sorbitol ...
-
bioRxiv - Physiology 2024Quote: ... slides were incubated at room temperature for 6 hours in staining solution containing 15 mM citrate buffer pH 5.3 (Sigma Aldrich), 2.8 mM CuSO (Sigma Aldrich) ...
-
bioRxiv - Neuroscience 2024Quote: LUHMES-derived DA neurons (day 6 of differentiation) were treated with a combination of antimycin A (25 µM, Sigma-Aldrich), and oligomycin (10µM ...
-
bioRxiv - Neuroscience 2024Quote: ... The samples were then blocked in 6% donkey serum and immunolabeled for protein gene product 9.5 (PGP9.5, 1:500, Millipore-Sigma, SAB4503057-100UG) as a pan-neuronal marker or rabbit-IgG (Abcam ...
-
bioRxiv - Biochemistry 2024Quote: A DNA consisting of 2 base pairs-Widom 601 sequence (145 base pairs)-2 base pairs was amplified by PCR using a 5’-/6-FAM forward primer (Sigma). The PCR products were pooled from four 96-well PCR plates (100 µL per well ...
-
bioRxiv - Bioengineering 2024Quote: ... 10 mL of MSC supplement and 6 mL of LAlanyl-L-Glutamine (ATCC, PCS-500-040) and 0.2 mg/mL G418 (Sigma, G8168) were included as medium supplements ...
-
bioRxiv - Systems Biology 2024Quote: ... Routine cells passaging was carried out at a 1:6 ratio every 2 days using 1× Trypsin–EDTA solution (Sigma) at 37°C ...
-
bioRxiv - Microbiology 2024Quote: ... the nanoparticles were washed by centrifugation at 20,000 g once and then re-dispersed in 2 mL of a 6 mM sodium citrate solution (Sigma-Aldrich). Next ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were plated in in 24-or 6-well plates previously coated with Poly-L-ornithin (0,1 mg/ml, Sigma-Aldrich) at a density of 250,000 cells/mL ...
-
bioRxiv - Neuroscience 2024Quote: ... Mice were acclimated to an isolated behavior space for 1hr prior to gavage with 100µl of sterile carmine red dye (6% w/v) (Sigma, C1022) dissolved in 0.5% methylcellulose (Sigma ...
-
bioRxiv - Immunology 2024Quote: ... Hyde) were cultured for 4 days at 37ºC in BSK-II media and complete with 6% rabbit serum (Millipore Sigma) at pH of 7.6 ...
-
bioRxiv - Cell Biology 2024Quote: ... the cell layers were washed with 200 µL of 6 M Guadinium Hydrochloride (Gdn-HCl, Sigma, Cat no. G3272-25G), 5 mM EDTA (Fluka ...
-
bioRxiv - Immunology 2024Quote: ... each microfluidic device was filled with 60 µL of a hydrogel formulation containing the following components: 6 µL of 10x phosphate-buffered saline (PBS) (#5493, Sigma), 6 µL of 0.1 M sodium hydroxide (NaOH ...
-
bioRxiv - Molecular Biology 2024Quote: ... HRS/Gsc-GFP dual reporter cells at day 6 of the ADE differentiation were isolated by washing the cells twice with PBS (Sigma; suitable for cell culture ...
-
bioRxiv - Molecular Biology 2024Quote: ... were treated with 50 µL of a 1mg/ml Concanavalin A (prepared in 10 mM sodium phosphate buffer pH 6, 10 mM CaCl2 ,1 mM MnCl2, 0.01% NaN3, Catalog C2010, Sigma Aldrich) solution ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... the NPSH levels in the samples (50 µg of protein) were mixture of 50 µL of 6% TCA (Sigma Aldrich). Subsequently ...
-
bioRxiv - Cell Biology 2024Quote: ... samples were washed in 1X PBS and incubated with DAPI (4-6-diamidino-2-phenylindole, Sigma-Aldrich, 1 µg/mL) for 10 minutes at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: ... the respective secondary antibodies were incubated in ADB at room temperature in the dark for 90 min together with 0.5 µg/mL 4′,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich). Before imaging the cells were washed again using PBS ...
-
bioRxiv - Cell Biology 2024Quote: ... Expanded colonies were tested by inducing ER stress with 200 nM thapsigargin for 6 h (Sigma-Aldrich, Burlington, MA, USA) using confocal microscopy (Zeiss LSM800 controlled by the ZEN 2.3 software ...
-
bioRxiv - Immunology 2024Quote: ... the slices were cultured for 4-6 days onto culture membranes (uncoated 30 mm Millicell-CM tissue culture inserts with 0.4 mm pores, Merck Millipore, Germany) and kept in six-well dishes (BD Biosciences ...
-
bioRxiv - Cancer Biology 2021Quote: ... 20 μl of 5mg/ml MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Sigma) was added to each well ...
-
bioRxiv - Cancer Biology 2021Quote: ... HsNHE9 targeting short hairpin RNA (shRNA) (5’-CCGGCCCTCCATTAAGGAGAGTTTTT CAAGAGAAAACTCTCCTTAATGGAGGTTTTTC-3’) and scramble control (Sigma-Aldrich) (5’-CAACAAGATGAAGAGCACCAA-3’ ...
-
bioRxiv - Cancer Biology 2021Quote: ... slides were incubated with 0.16 M N-(3-Dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) (Sigma Aldrich) in the 1-methylimidazole solution for 1 hour at RT to preserve small RNAs21 ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1 mM EDTA) at 4 °C for 10 min before 3% of Ficoll-400 (Sigma) was added and assembled Nucleolin complexes were resolved on a 10% TBE PAGE gel at 4 °C.
-
bioRxiv - Cell Biology 2019Quote: ... supplemented where necessary with the addition of 30 nM 3-isobutyl-1-methylxanthine (IBMX; Sigma) to arrest oocytes at prophase I ...
-
bioRxiv - Cell Biology 2020Quote: ... and 7 μL of 100 mg/mL glyceraldehyde-3-phosphate (Cayman Scientific or Sigma Aldrich) from frozen stocks ...