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Citations for Millipore Sigma :
7051 - 7100 of 10000+ citations for 6 PHENYL 1 3 DIHYDRO INDOL 2 ONE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: Adult male frogs (n = 2) were anesthetized with 1% MS-222 (tricaine, Sigma-Aldrich, St. Louis, MO, USA) and sacrificed by decapitation ...
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bioRxiv - Cell Biology 2021Quote: ... topped up to 140 ug/ul for the final 2 h and then DNA was extracted with 20 ml Phenol:Chloroform:Isoamyl Alcohol 25:24:1 (Sigma), precipitated with ethanol ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed for 30 min at 4°C on the wheel with RIPA buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1% NP-40, 0.5% deoxycholic acid, 0.1% SDS, 2 mM MgCl2; Sigma-Aldrich) supplemented with proteases and phosphatases inhibitors and 50 mM 2- Chloroacetamide to inhibit the de-ubiquitinases ...
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bioRxiv - Cancer Biology 2020Quote: ... The pETDuet-1-His-BAP1-ULD+ASXL2-AB protein complex was expressed in Rosetta 2 (DE3) pLysS (Millipore). The bacteria bearing the desired plasmids were propagated with aeration at 37°C in 1L of 2YT to an A600 absorbance of approximately 0.6 ...
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bioRxiv - Microbiology 2020Quote: ... HIV-GFP produced with p8.91 or p8.2 used in Figure 1 were DNase treated for 2 hours at 37°C (DNaseI, Sigma) prior to ultracentrifugation ...
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bioRxiv - Microbiology 2021Quote: ... and 0.5 µg pcDNA3.1 SARS-CoV-2 Spike D614 in OptiMEM with 10 µl polyethyleneimine 1 µg/ml (Sigma) as previously described (Conceicao C ...
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bioRxiv - Neuroscience 2020Quote: ... A borosilicated glass micropipette was filled with NES and 2 mM Glutamate or 1 mM GABA (Sigma Aldrich), and moved via a micromanipulator MP-225 (Sutter Instruments ...
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bioRxiv - Cancer Biology 2021Quote: ... Filtered cells were immediately seeded in 96 well plates coated with 1% Poly (2-hydroxyethyl methacrylate; Sigma Aldrich) at a density of 200 or 50 cells per well ...
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bioRxiv - Cell Biology 2021Quote: A solution of methimazole (1-methyl-3H-imidazole-2-thione) (CAS 60-56-0; MW: 114,17; Sigma-Aldrich) was used to induce hypothyroidism ...
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bioRxiv - Cell Biology 2020Quote: ... the hSCOs were either cultured in DM containing AEDs (valproic acid, 0.5 or 1 or 2 mM, Sigma, P4543 ...
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bioRxiv - Biochemistry 2022Quote: ... Fatty acids were extracted using a chloroform-methanol mix (2:1; Thermo-Fisher) in 0.01% butylated hydroxytoluene (Sigma) prior to centrifugation at 200 g for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: A375 cells in 100 mm dishes were challenged by 2 mM of octanoate-1-13C (Sigma Aldrich #296457) for 6 or 20 hrs ...
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bioRxiv - Cell Biology 2022Quote: Evaporated lipids were resuspended in 10µL 2:1 chloroform/methanol and spotted on dehydrated silica plates (EMD Millipore; dehydrated by baking for 30 minutes at 100°C) ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were incubated for 2 h at RT with anti-αSMA antibody (1:200, Sigma Aldrich, C6198-100UL) and Goat anti Rabbit Alexa Fluor 647 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... were incubated with secondary antibodies for 2 h: Alexa Fluor 488 goat anti-mouse IgG (1:400, Millipore), or Fluor 594 goat anti-rabbit IgG (1:400 ...
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bioRxiv - Biophysics 2020Quote: ... The quenched samples were concentrated to 60 μl by centrifugation at 20,000xg for 2 min (Sigma 1-16K). Due to the linear nature of DNA ...
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bioRxiv - Neuroscience 2022Quote: ... samples were stained with the fluorescent nucleic acid marker 4,6-diamino-2-fenilindol (DAPI 1:3000; Sigma-Aldrich), and mounted with a Fluorescent Mounting Medium (DAKO ...
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bioRxiv - Molecular Biology 2020Quote: ... at a 2:1 ratio of beads to reaction volume with an additional 1.8x supplementation of isopropanol (Sigma).
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bioRxiv - Genetics 2021Quote: ... the resin with the immobilized MBP-Rif2 variants was first washed 4 times with 1 ml wash buffer containing 1M NaCl (50 mM Tris-HCl pH 7.5, 1 M NaCl, 0.2% NP40, 2 mM EDTA, Sigma protease inhibitory cocktail P8340 1:1000) ...
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bioRxiv - Cancer Biology 2021Quote: ... universal MISSION® siRNA Universal Negative Control #1 (cat. SIC001) and #2 (cat. SIC002) were from Millipore Sigma. We used the DharmaFECT 4 Transfection Reagent (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Tissues were hybridized overnight at room temperature with the B-5-1-2 monoclonal anti-α-tubulin (Sigma) and the anti-KNOLLE antibody (kind gift of G ...
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bioRxiv - Physiology 2020Quote: ... following which they were incubated with anti-MAP-2 antibody (AB5543, 1:500, Merck Millipore, Burlington, MA, USA) in blocking buffer overnight at 4℃ ...
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bioRxiv - Biochemistry 2021Quote: ... resin was incubated with 2 bed vol of PBS containing 10 μL of 1 U/μL thrombin (Sigma) for 2 h at 25°C with occasional agitation ...
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bioRxiv - Biochemistry 2021Quote: ... The cellular suspension was distributed in portions of 0.8 millilitres to reaction tubes with 2 millilitre volume to which 1.4 grams of glass beads (0.75 - 1 millimetre, Sigma) were added ...
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bioRxiv - Molecular Biology 2021Quote: ... see Table 1 for complete plasmid list) was induced by treating cells with 2 µg/mL doxycycline (Sigma) for at least 72 hours ...
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bioRxiv - Biophysics 2021Quote: ... then washed and resuspended in the same volume of YEPD (2% glucose) medium containing 1 mM auxin (Sigma). These cells were grown in the presence of auxin for another 20 minutes at 30 °C to deplete Rbg1 completely.
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bioRxiv - Microbiology 2020Quote: ... The SDS-PAGE gel was stained for 2 h using staining solution [Brilliant Blue (1 g; Sigma-Aldrich), methanol (50% v/v ...
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bioRxiv - Immunology 2021Quote: ... RPMI was replaced with 2 mL enzymatic solution of CO2-independent medium containing 1 mg/mL liberase (Sigma) and 20 μg/mL DnaseI (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... lysogens were grown to 0.1 – 0.2 OD600 and 2 μg ml-1 mitomycin C (Sigma, from Streptomyces caespitosus) added to induce resident prophages ...
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bioRxiv - Developmental Biology 2022Quote: ... Tubes containing the ovomucin preparation were diluted 1:2 with pH 7.0 to 9.3 buffers before the addition of purified lysozyme (2ug/ml, Sigma). The contents were then mixed and incubated at 37°C for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... 1:1000 in blocking buffer) and 0.5–0.8 μg/mL DAPI (4,6-diamidino-2-phenylindole, Sigma-Aldrich, D9542) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... β-mercaptoethanol and HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid] were procured from Sigma (St. Louis, MO, USA). Urea and proteinase-K were bought from Amresco ...
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bioRxiv - Cell Biology 2022Quote: ... fixed with 2% paraformaldehyde in PBSP (1x PBS pH7.4, 1 mM DTT, 1x Sigma plant protease inhibitor cocktail) for 30 min on ice ...
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bioRxiv - Evolutionary Biology 2022Quote: ... corneas were resected and homogenized by incubation in 1mL of buffer containing 2 mg/mL collagenase 1 (Sigma) for 1 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed three times and incubated in 4,6-diamidino-2-phenylindole (DAPI) (1 µg/ml; Sigma-Aldrich) for 5 min ...
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bioRxiv - Physiology 2022Quote: ... a 4 uL suspension of 2 μm red-fluorescent polystyrene microspheres (Sigma-Aldrich; 1:1000 dilution in PBS) was apically applied to native mucus ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were reduced and alkylated in digestion buffer containing 1:50 (v:v) tris(2-carboxyethyl)phosphine (0.5 M, Sigma) and 1:10 (v:v ...
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bioRxiv - Neuroscience 2022Quote: ... by 1:10 dilution in a vehicle solution 10% w/v 2-hydroxypropyl-β-cyclodextrin in PBS (Sigma). Alternatively ...
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bioRxiv - Molecular Biology 2024Quote: ... 10-25 μg of chromatin was incubated with 1-2 μg of antibodies (Abcam: 9E10, Y69, ab2739, Millipore: 04-835 ...
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bioRxiv - Neuroscience 2024Quote: ... Both groups were treated with WIN55,212-2 (1 nM or 10 nM) (Sigma-Aldrich, St. Louis, MO, USA) dissolved in ethanol ...
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bioRxiv - Cell Biology 2024Quote: ... followed by staining with a 1% alizarin red S solution (200 μL/well; Sigma-Aldrich, #01-2180-2) at pH6.4 ...
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bioRxiv - Neuroscience 2023Quote: ... and a biotinylated donkey anti-rabbit immunoglobulin G secondary antibody (1:1,000 for 2 hours at RT; Millipore). To eliminate nonspecific binding prior to immunostaining endogenous peroxidase activity was blocked using 0.3% H2O2 for 30 min at room temperature (RT) ...
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bioRxiv - Plant Biology 2023Quote: ... and reduced and alkylated by addition of digestion buffer containing 1:50 (v:v) tris(2-carboxyethyl)phosphine (0.5 M, Sigma) and 1:10 (v:v ...
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bioRxiv - Synthetic Biology 2023Quote: ... and a titration of trimethoprim (0, 1, 2, 4, 8, 16, 24, and 32 µg/mL) (Sigma Aldrich) with 0.4 % dimethylsulfoxide ...
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bioRxiv - Molecular Biology 2022Quote: ... Chromatin in embryos was stained with Hoechst 33343 (1:5000 dilution in PBS, 2 μg/ml, Sigma-Aldrich) for 15 minutes.
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bioRxiv - Cell Biology 2023Quote: ... Tissues were incubated overnight at room temperature with the B-5-1-2 monoclonal anti-α-tubulin (Sigma) and the anti-KNOLLE antibody (kind gift of G ...
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bioRxiv - Neuroscience 2023Quote: ... The eyes were then placed in a 2:1 solution of PolyFreeze Tissue Freezing Medium (TFM) (Sigma-Aldrich) and 30% sucrose for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Aβ40-E22Q was dissolved to 1 mM in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP; Sigma, St. Louis, MI, USA), incubated for 24h to breakdown pre-existing β-sheet structures and lyophilized ...
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bioRxiv - Cell Biology 2023Quote: ... containing 10 µg/ml LPC (leupeptin, Pepstatin A, and chymostatin) and 1% phosphatase inhibitor cocktail 2 (P5726, SIGMA). Following clarification of the lysate by centrifugation at 15,000 rpm (21,000 g ...
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bioRxiv - Immunology 2023Quote: ... Then the wells were washed 2 times with PBS and 200 μl of SLC3A2 (1: 500) (Sigma / HPA017980) primary antibody solution (in 5%BSA ...