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Citations for Millipore Sigma :
7001 - 7050 of 10000+ citations for 6 PHENYL 1 3 DIHYDRO INDOL 2 ONE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... T cells transduced with the anti-hCD19 CAR construct 42 were cultivated for additional 2 days in presence or without 2 mM 2-DG (Sigma) before adoptive transfer into MC38-hCD19 tumor-bearing mice.
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bioRxiv - Developmental Biology 2023Quote: ... The following factors were applied to experimental BPs in culture at the specified concentrations: 2-Deoxyglucose (2-DOG) 2 mM (SIGMA), Sodium Pyruvate (NaP ...
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bioRxiv - Biophysics 2023Quote: ... different amounts of freeze dried GelMA macromer were dissolved in DPBS containing 0.5% (w/v) 2-Hydroxy-4’-(2-hydroxyethoxy)-2- methylpropiophenone (Sigma-Aldrich) as photoinitiator ...
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bioRxiv - Molecular Biology 2021Quote: ... Cordycepin was added to the 3’ end of in vitro transcribed mRNA by substituting 50 mM Cordycepin (3’-deoxyadenosine) 5’-triphosphate sodium salt (Sigma-Aldrich) in 10 mM Tris pH7 for ATP in a poly(A)-tailing reaction with a Poly(A ...
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bioRxiv - Neuroscience 2020Quote: ... the eluate was washed 3 times with PBS in an Amicon Ultra-4 Centrifugal Filter Unit (NMWL 3 kDa, Millipore, UFC800324) and finally concentrated to < 250 µl ...
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bioRxiv - Cancer Biology 2020Quote: ... PRL-3 over expressing construct was made by cloning full length PRL-3 cDNA into p3XFLAG-CMV-14 expression vector (Sigma, E7908)
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bioRxiv - Neuroscience 2020Quote: Human cofilin1 was amplified by PCR using specific oligonucleotides (forward: 5’-CATATGGCCTCCGGTGTG-3’, reverse: 5’-GGATCCTCACAAAGGCTTGCCCTC-3’) and cloned into the pET-15b vector (Novagen, Millipore, UK). By using site-directed mutagenesis ...
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bioRxiv - Immunology 2022Quote: ... Cells were exposed to LPS (100 ng/mL) for 3 hr with ethanol or HNE (0.3-3 µM, Millipore Sigma, 393204) in appropriate growth media ...
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bioRxiv - Biophysics 2021Quote: ... was performed using a pre-annealed DNA-RNA template (DNA45: 5’-TTCTTT ATCTCTTATTTCCTTCTATTTTCCACGCGCCTTCTATTT-3’; RNA13: 5’-[6FAM]-GGCGCGUGGAAAA-3’, Sigma Aldrich). The reaction buffer comprised 25 mM HEPES pH 7.2 ...
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bioRxiv - Synthetic Biology 2020Quote: ... indole-3-propionic acid (order no. 57400) and indole-3-butyric acid (order no. i5386) have been obtained from Sigma-Aldrich. All ligands have been dissolved in 50mM Tris / 300mM NaCl pH8 buffer with if necessary 1% dimethyl-sulfoxide (DMSO) ...
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bioRxiv - Developmental Biology 2022Quote: ... Medium was refreshed 3 days after seeding and 3 days later cells were fixed with citrate/acetone/3% formaldehyde and stained with Leukocyte Alkaline Phosphatase Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... with either the wild type Cacna2d2-3’UTR or the Cacna2d2 3’UTR bearing mutations in the miR-423-5p binding sites (Cacna2d2 mut 3’UTR) in HEK 293 cells (Millipore Sigma). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... 53 from the RNAi Consortium (clones TRCN0000087290 and TRCN0000087292 with target sequence 5’-GCGGGAGTATAGTGAGTTTAA-3’ and 5’-CGGACAGTTTGGCACAATCAA-3’, respectively, distributed by Sigma-Aldrich, Merck ...
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bioRxiv - Neuroscience 2022Quote: ... 3 x 10 min 100% EtOH) and cleared (3 x 5 min) in xylene (247642, CAS: 1330-20-7, Sigma Aldrich). Finally ...
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bioRxiv - Physiology 2023Quote: Tissue from the LV of 3 sedentary and 3 exercised mice of each sex was fixed in RNAlaterTM (Millipore Sigma, MFCD03453003), and RNA sequencing was performed by Genewiz from Azenta Life Sciences ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: ... Slides were incubated for 3 minutes in 3% acetic acid and then for 20 minutes in Alcian blue solution (Sigma Aldrich) at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The bacterial DNA was used to amplify the entire 16S region by PCR [5′-AGAGTTTGATCCTGGCTCAG-3′ (forward) and 5′-AAGGAGGTGATCCAGCCGCA-3′ (reverse)] using Expand High-Fidelity Polymerase (Sigma-Aldrich). The PCR products were purified using the QIAquick PCR Purification kit (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... a shRNA sequence based on 5’-GCCTAAATGGTCAAGGAGATA-3’ as the sense nucleotide corresponding to the 3’UTR of mouse mDia1 (NM_007858.4; Sigma-Aldrich, Cat# TRCN0000108685) was designed as an oligonucleotide with overhangs (s ...
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bioRxiv - Biochemistry 2023Quote: Two complementary single strand oligonucleotides (5’-GTAATCTGGGCCACCTGCCTGGGAGGA-3’ and 5’-TCCTCCCAGGCAGGTGGCCCAGATTAC-3’) corresponding E2 box44 were Two complementary single strand oligonucleotides (5’-purchased from Sigma-Aldrich. An equal molar ratio of single strand oligonucleotides was mixed and incubated at 95°C for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... fixed brains were washed 3×20 min with 0.1% PBT and incubated in 3% normal goat serum (NGS; Sigma-Aldrich; G9023) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Immunolabelling was performed overnight at 4°C with primary antibodies (Table 3) in blocking solution containing 3% normal goat serum (Sigma-Aldrich) and 0.3% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... The Oil Red O stain was prepared by mixing 3 parts of 3 mg/mL Oil Red O (Sigma-Aldrich, O0625) in 99% isopropanol with 2 parts deionized water ...
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bioRxiv - Immunology 2020Quote: ... for 2 days and then just IL-2 (20ng/ml) (Sigma-Aldrich) for a further 5 days ...
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bioRxiv - Developmental Biology 2021Quote: ... the tissues were processed to 2’-2’-thio-diethanol (TDE, Sigma, 166782) for clearing and mounting ...
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bioRxiv - Immunology 2022Quote: ... 5 μM oligomycin and 100 mM 2-deoxyglucose (2-DG) (Sigma-Aldrich) were injected to each well after 18 ...
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bioRxiv - Immunology 2021Quote: ... 2-Deoxy-D-glucose (2-DG) was obtained from Sigma Aldrich (D8375) and used at 0.32 mM ...
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bioRxiv - Genomics 2020Quote: ... Substrate solution (2 mM ABTS, Sigma A9941; 2 mM hydrogen peroxide, Sigma H1009 ...
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bioRxiv - Cell Biology 2021Quote: ... The last injection was supplemented with 2-deoxyglucose (2-DG, Sigma, D8375) to obtain the ECAR background value due to nonglycolytic acidification ...
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bioRxiv - Immunology 2021Quote: ... For the last 2 h 2 µg/ mL Brefeldin A (Sigma-Aldrich) was added ...
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bioRxiv - Immunology 2021Quote: ... 2 ml overlay [MEM containing 2% FBS and 0.4% tragacanth (Sigma, Israel)] were added to each well and plates were further incubated at 37°C ...
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bioRxiv - Immunology 2020Quote: ... 55μM 2-mercaptoethanol (2-ME) supplemented with 50 U/ml Benzonase (Sigma). Aliquots derived from the same donors were combined and all samples were enriched for T cells by removing CD14 and CD19 expressing cells using a column-based magnetic depletion approach according to the manufacturer’s recommendations (Miltenyi) ...
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bioRxiv - Immunology 2021Quote: ... cells were treated with 2-deoxyglucose (2-DG) 100 nM (Sigma, #D8375).
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bioRxiv - Immunology 2020Quote: ... 2 ml overlay [MEM containing 2% FBS and 0.4% tragacanth (Sigma, Israel)] were added to each well and plates were further incubated at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µM epoxomicin and 50 µM (Z-LL)2-ketone (Merck Millipore) were added from stock solutions in dimethyl sulfoxide (DMSO) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1µM Oligomycin A and 50mM of 2-deoxyglucose (2-DG) (Sigma, D3179). For mitostress tests ...
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bioRxiv - Biochemistry 2024Quote: ... oligomycin and 2-deoxy-D-glucose (2-DG, Sigma-Aldrich, D8375-1G)) at final well concentrations of 10 mM ...
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bioRxiv - Developmental Biology 2024Quote: ... dissolved in water and 2-2’ bipyridyl (BP) (Sigma‒Aldrich Cat# D216305) dissolved in DMSO were added to the NGM before pouring to final concentrations of 4 mmol/L ...
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bioRxiv - Bioengineering 2024Quote: ... methyl triflate and 2-ethyl-2-oxazoline were obtained from Sigma Aldrich and dried over CaH ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 2.5% v/v 2-mercaptoethanol (Sigma-Aldrich, #60-24-2), heated at 95°C for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... 4- Methylumbelliferyl-2-Acetamido-2-Deoxy-β-D-Glucopyranoside (MUG, Sigma Aldrich) or 4- Methylumbelliferyl-6-sulfo-N-acetyl-β-D-glucosaminide (MUGS ...
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Multi-scale Assessment of Brain Blood Volume and Perfusion in the APP/PS1 Mouse Model of AmyloidosisbioRxiv - Neuroscience 2022Quote: ... the CUBIC solution 1 was washed away with 6 PBS washes over the course of 6 hours and the brain tissue was then incubated overnight at RT with a 1:1 mix of PBS and CUBIC solution 2 (50 % sucrose, 25 % urea and 10 % TEA [Triethylamine, Sigma-Aldrich] weight/weight in MiliQ). The next morning ...
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bioRxiv - Microbiology 2023Quote: ... After 3 washes in TBST the membranes were incubated in secondary antibodies for 2 h at room temperature (Goat α-rabbit HRP 1:1000 - Sigma Aldrich/ GERPN4301, Goat α-mouse HRP 1:1000– Sigma Aldrich/GERPN4401), revealed using ECL reagent (BioRad ...
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bioRxiv - Microbiology 2024Quote: ... washed in water until the stain turned blue approximately 2 mins and then counter stained with 1% Eosin (Sigma-Aldrich, Cat. no. 17372-87-1) for 30 Sec ...
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bioRxiv - Neuroscience 2024Quote: ... Under anesthesia by intramuscular injection of 0.4 mL of a 1:1 mixture of ketamine (10 mg/mL, Daiichi-Sankyo Pharma Co., Tokyo, Japan) and xylazine (2 mg/mL, Sigma-Aldrich Co., St Louis, USA), the left hemisphere was dissected and immersed in ice-chilled Krebs solution composed of (in mM) ...
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bioRxiv - Genetics 2021Quote: ... 2 mM EDTA and 1% Triton X-100) with 20 mg/mL lysozyme from chicken egg (Sigma, L4919) using a tissue grinder (BMS BC-G10 ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... t Phospho-Myosin Light Chain 2 (Ser19) antibody (3671; Cell Signalling) 1:50 and Recoverin antibody (AB5585; Millipore) 1:100 ...
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bioRxiv - Cell Biology 2022Quote: ... and 10 µg ml−1 of L-ascorbic acid or 2-Phospho-L-ascorbic acid trisodium salt (Sigma) (sigma) ...
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bioRxiv - Immunology 2022Quote: ... All wells were treated for 1 hour with 10 µM 2’,7’-dichlorofluorescin diacetate (DCF-DA, Sigma, D6883) in Ringer buffer (155 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... diluted with fresh medium (1/2) and hexadimethrine bromide was added (final concentration 8 μg/ml; Sigma-Aldrich). Target cells were then infected ...
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bioRxiv - Biophysics 2022Quote: ... for 30 min and blocked for at least 2 h with a blocking buffer containing 1% BSA (Sigma), 3% donkey serum (Millipore) ...