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Citations for Millipore Sigma :
7001 - 7050 of 10000+ citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... blocked in 5% BSA for 30 min and incubated ON with mouse anti-GAD67 monoclonal antibody (1:6000, MAB5406, Merck Millipore) or 1 h with rabbit polyclonal anti-GAPDH antibody (1:3000 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 0.05% (v/v) Tween-20 in PBS) and incubated with primary antibody anti-LIG3 (1:150, Sigma-Aldrich, #HPA006723) in staining buffer for 2hr at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... cells expressing GIPR and GLP-1R were incubated with 1:300 anti-Flag primary antibody (diluted with PBS containing 5% BSA, Sigma), and those expressing GCGR were incubated with 1:50 anti-GCGR antibody (diluted with PBS containing 5% BSA ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were washed in PBS and blocked in blocking buffer (3% (w/v) BSA in PBS) and the primary antibody applied at the relevant dilution in 1% (w/v) BSA (Sigma) in PBS and incubated overnight at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Insoluble material was removed by centrifugation at 18,000 x g and the antibody was concentrated to 1 mg/mL using a Centricon spin concentrator (Millipore Sigma). Purified polyclonal antibody was verified for reactivity against recombinant purified NOCT (64-431 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Expression of GAPDH was used as a loading control using a mouse anti-GAPDH antibody (1:10000 dilution, MAB374, Sigma) and IRDye 680CW goat anti-mouse IgG secondary antibody (1:5000 dilution ...
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bioRxiv - Pathology 2021Quote: ... postmortem kidney was fixed in 20% formalin for 2 weeks and processed for free-?oating preparation as done for mouse tissue with one exception that anti-human CD31 antibody (CBL468, 1:20 dilution, Millipore) was used.
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bioRxiv - Cell Biology 2020Quote: ... and the mouse monoclonal and rabbit polyclonal anti-phosphorylated histone H3 (Ser10) antibodies (ref. 06570, 1:1000) were obtained from Millipore. The anti-GFP mouse monoclonal antibody mix (ref ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated overnight at 4°C in blocking solution containing one of the following primary antibodies: anti-GFAP (1:2000; Merck Millipore) and anti-Iba1 (1:500 ...
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bioRxiv - Plant Biology 2020Quote: ... were isolated from 2-week-old dPPRrbcL-1 or Col-0 plants and incubated with monoclonal anti-HA antibodies (Sigma). IgGs were captured with Protein A DynaBeads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... plates were again washed thrice followed by incubation with 1:5,000 dilution of horse radish peroxidase (HRP) conjugated anti-guinea pig IgG secondary antibody (Sigma-Aldrich) or 1:2,000 dilution of HRP conjugated anti-mouse IgG secondary antibody (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Protein level of GTP-bound ARF6 and total ARF6 in cell lysates was detected by Western blot with an anti-ARF6 antibody (1:1000 in 5% milk) (Sigma).
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bioRxiv - Cell Biology 2019Quote: ... the membrane was incubated with either α-D-TPX2 antibody at 1:2000 dilution or α-tubulin (DM1-A, Sigma) antibody at 1:10,000 dilution for 1 hour ...
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bioRxiv - Plant Biology 2019Quote: ... the blot was washed with 1×TBS-T solution (4×5min) and incubated with anti-Mouse IgG-HRP secondary antibody solution (Sigma, 1:10,000 dilution in 1×TBS-T with 0.2% Blocking Reagent [Roche] ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were resolved in SDS-PAGE gels and transferred to nitrocellulose membranes that were incubated overnight at 4 °C with one of the following antibodies: mouse monoclonal anti-HA (1:2000, Sigma); rabbit polyclonal MBP-probe (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... Nuclei were then labeled in a solution of anti-NeuN antibody conjugated to Alexa Fluor 488 (A60, Millipore, 1/1000) and 0.1% BSA ...
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bioRxiv - Biochemistry 2020Quote: ... HA-tagged [P2G]CXCL12 was detected using a rabbit anti-HA primary antibody (1:10,000 dilution; Sigma Aldrich, catalog #SAB4300603) and IRDye® 800CW-conjugated goat anti-rabbit IgG (1:20,000 dilution ...
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bioRxiv - Physiology 2020Quote: ... and probed overnight (4 °C) with one of the following primary antibodies: Anti-TSG-6 mouse monoclonal (1 μg/ml; Millipore); rat monoclonal TNFα (1 μg/ml ...
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bioRxiv - Cell Biology 2019Quote: ... were coated non-covalently with the antibodies of interest or covalently to Poly-D lysine (Sigma; 200 μg ml-1). They were washed with phosphate buffered saline containing 0.1% (w/v ...
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bioRxiv - Bioengineering 2019Quote: ... Plates were then incubated with the respective primary antibody in PBS/0,1% Triton-X 100/1% bovine serum albumin (BSA; Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed then sequentially incubated with anti-SARS-CoV-2 CR3022 antibody (Yuan et al., 2020) (1 μg/mL) and a HRP-conjugated goat anti-human IgG (Sigma) in PBS supplemented with 0.1% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins on a gel were transferred to a nitrocellulose membrane and analyzed by using the antibodies at 1:3000-10,000 dilutions: DM1a (Sigma, #T9026), α-GFP (Roche ...
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bioRxiv - Physiology 2021Quote: ... Muscles were then incubated in blocking solution containing rabbit anti-heavy NF200 primary antibody (N4142 Sigma, Germany; 1:200 dilution) and anti-synaptophysin antibody (1:25 dilution as above) ...
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bioRxiv - Physiology 2020Quote: ... Immunofluorescence staining was performed using sheep anti-α-MSH antibody (diluted 1:1,000 in 0.1 M PB, ab5087, Millipore Sigma), and rabbit anti-AgRP antibody (diluted 1:1,000 in 0.1 M PB ...
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bioRxiv - Microbiology 2021Quote: ... An α-mouse IgG conjugated to horseradish peroxidase (HRP) was used as the secondary antibody at a dilution of 1:4,000 (Sigma-Aldrich). To assess cell wall fractionation a mouse α-protein A (Spa ...
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bioRxiv - Neuroscience 2021Quote: ... cells and sections were subsequently incubated with Alexa Flour 488/594/647-conjugated secondary antibodies and counterstained with DAPI (1:5000; Sigma). Immunofluorescence staining was examined using a confocal microscope.
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bioRxiv - Neuroscience 2020Quote: ... Samples were then resolved by SDS-PAGE and western blotting was carried out as described in (Hansen et al., 2014) using the following antibodies: DAT (rat MAB369, 1:1000, Millipore) or HA (rat 3F10 ...
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bioRxiv - Neuroscience 2021Quote: ... slices were washed with PBS/0.1% Triton X-100 (T-PBS) and incubated with the primary antibody NeuN (1:200, Sigma, ABN78) in T-PBS for 1 day at 4°C while shaking ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Sections were stained for NeuN immunohistochemistry using 1:10,000 polyclonal rabbit anti-NeuN antibody (ABN78; EMD Millipore; Temecula, CA, USA) and horse anti-rabbit secondary antibody via an HRP reagent (ImmPRESS MP-7401 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the membrane was incubated overnight at 4 °C with the first antibody (rabbit anti-Tc-Zen1 and anti-Tc-Zen2 as for immunohistochemistry, 1:1,000; mouse anti-Tubulin [Sigma-Aldrich #T7451 ...
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bioRxiv - Biochemistry 2021Quote: ... the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich) in PBST ...
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bioRxiv - Neuroscience 2021Quote: ... blocked in PBS containing 0.3% Triton X-100 and 10% normal donkey serum and incubated with primary antibody overnight at 4 °C overnight (Rockland, rabbit anti RFP, 1:500; Millipore, chicken anti-GFAP ...
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bioRxiv - Cell Biology 2021Quote: ... plates were washed 4 times with PBST and further incubated with 100 μL per well of goat anti-human IgG (Fc specific)-Peroxidase antibody (1: 5000 dilution, Sigma) for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed in 4% PFA and stained with a phospho-Histone H3 primary antibody (1:1000; Millipore #5-806) overnightat 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... The following antibodies at the indicated dilutions were employed in western blot analysis: rabbit polyclonal anti HA (SIGMA, 1:3000), mouse monoclonal anti HA (Cell Signalling ...
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bioRxiv - Developmental Biology 2020Quote: ... Cryostat sections or whole embryos were stained using standard protocols and antibodies against the following antigens: HA (1:250, Sigma), β-catenin (1:300 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... followed by incubation with a secondary Cy3-labeled polyclonal goat anti-rabbit antibody (AP132C, 1:800, Merck Millipore, Burlington, USA). Cells were mounted on slides using Fluoroshield™ with DAPI mounting medium (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... The mouse anti-GFP-20 antibody was used in GRASP and synaptotagmin experiments (1:100 in PBST-NGS; Sigma-Aldrich); the mouse nc82 antibody was used in synaptotagmin::GFP and DenMark experiments ...
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bioRxiv - Microbiology 2020Quote: rAkata EBV-infected cells was reactivated at 1×106 cells/mL with a goat polyclonal anti-human IgG Fc-specific antibody (Sigma) for 48 hours ...
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bioRxiv - Microbiology 2020Quote: ... The permeabilized cells were washed with PBS and sequentially incubated anti-DENV primary antibody (dilution 1:400 of Mab8705, Millipore) and anti-Mouse Alexa-488 conjugated secondary antibody (dilution 1:500 ...
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bioRxiv - Microbiology 2021Quote: ... milk powder in TBS-T (Tris-buffered saline-Tween-20) and then incubated overnight with primary antibody (monoclonal anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG ...
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bioRxiv - Immunology 2020Quote: ... The sections were blocked in PBS containing 1% bovine serum albumin (BSA) for 1 h at RT and stained in blocking buffer containing primary antibody (anti-PP6C, Merck Millipore cat ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2h incubation with Alexa Fluor-488 goat anti-rabbit IgG secondary antibody (1:500, A11008, Merck Millipore, MA). Slices were transferred to glass slides and covered with Fluoromount (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated with mouse monoclonal Alexa Fluor-488 conjugated antibody against NeuN (1:200, MAB377X, Merck Millipore, MA, USA) or the rabbit polyclonal primary antibody against DISC1 (1:250 ...
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bioRxiv - Immunology 2020Quote: ... TZM-bl cells (1 X 104) were added into the mixture virus-antibody mixture containing 25 μg/ml DEAE-dextran (Sigma). The plates were further incubated for 48 h and the extent of virus neutralization was assessed by measuring relative luminescence units in a luminometer (Victor X2 ...
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bioRxiv - Physiology 2020Quote: ... The presence of LPL in the conditioned media was confirmed through Western blotting using a mouse antibody against the FLAG tag (1:5000; Sigma). LPL activity was assessed through an LPL activity assay (see below).
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bioRxiv - Genetics 2019Quote: ... Their expression levels were normalized using the FLAG staining (FLAG antibody: 1:1,000; F1804, Sigma-Aldrich, Saint-Louis, MO, USA).
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bioRxiv - Biochemistry 2020Quote: ... the membrane was washed and incubated with primary antibody diluted in 5% BSA/TBST (1:10,000 anti-Flag (Sigma-Aldrich) or anti-Beta lactamase (Abcam) ...
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bioRxiv - Cell Biology 2021Quote: ... The following antibodies were used for immunoblotting: The HA epitope was recognised with a primary mouse monoclonal anti-HA (1:1000; Sigma); the Myc epitope was recognised with a primary mouse monoclonal anti-Myc (1:5000 ...
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bioRxiv - Cell Biology 2020Quote: ... We incubated the membranes overnight at 4°C in constant agitation with anti-Hsp23 antibody (1:1000) (Sigma-Aldrich S0821), anti-Hsp26 (1:1000 ...