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Citations for Millipore Sigma :
6801 - 6850 of 10000+ citations for Type 1 Angiotensin II Receptor AGTR1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: The chvI and mirA coding sequences (wild type and mutant alleles) were amplified from cloning constructs (primer sequences in Table 2) and ligated into pET-28a(+) (Novagen/ EMB Biosciences/ Millipore). Protein-expression plasmids were transformed into E.coli Top10 F’ for collection and sequence verification and then transformed into E.coli BL21/λDE3 for expression and protein purification ...
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bioRxiv - Developmental Biology 2021Quote: ... the cumulus cells were dispersed by brief treatment with hyaluronidase (Type I-S, 120-300 units/mL; Sigma-Aldrich, St Louis, MO, USA). A total of 30 female and 6 male mice were used ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... after perfusion of the pancreas through the common bile duct with 5-10mL of calcium-free Hanks-buffered saline solution (HBSS) supplemented with 0.4mg/mL Type V collagenase (C9263, Sigma, St. Louis, MO, USA). After overnight culture ...
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bioRxiv - Physiology 2021Quote: ... Cell pellets were resuspended in growth medium and plated in tissue culture dishes coated with type I rat tail collagen (Sigma, St. Louis, MO). Cultures were expanded and frozen at passage 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... 2.105 Met-5A cells were plated in Ibidi µ-Slide 8 Well (Clinisciences #80826) coated with collagen type I (Sigma-Aldrich #C3867-1VL) and incubated for 48h at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... were dissected from E13.5 wild-type mice and plated in a 8-wells chamber coated with 10µg/ml poly-d-lysin (Sigma-Aldrich, St Louis, MO) and 10µg/ml mouse Laminin (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2022Quote: ... Wild type C57BL/6 mice were sacrificed and were perfused ventricularly with sodium heparin solution in PBS (H3393-100KU, Sigma-Aldrich, MO, USA). The lungs were then excised and fixed in 4% PFA overnight at 4°C ...
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bioRxiv - Genetics 2020Quote: Exopolyphosphatase activity of wild-type PRUNE1 on medium and long chain PolyP substrates (consisting of 45, 65 and 700 orthophosphate monomers, Sigma S4379, Sigma S6253, Kerafast EUI002) was assessed by comparing to S ...
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bioRxiv - Biochemistry 2021Quote: The ATPase activity of wild-type LonP1 and its mutants was measured with ATPase/GTPase activity assay kit (Sigma-Aldrich, USA, product: MAK113) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: DNA encoding wild-type and mutant versions of sLro1 were cloned into the BamHI and XhoI sites of pET22b vector (Novagen, Merck, Darmstadt, Germany), which contains a PelB signal sequence to direct the secretion of the expressed protein into the periplasmic space ...
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bioRxiv - Cancer Biology 2021Quote: ... MDA-MB-231 cells from the American Type Culture Collection (ATCC; Gaithersburg, MD, USA) were cultured in Dulbecco’s modified Eagle medium (DMEM; Sigma-Aldrich; Oakville, ON, CA) supplemented with 10% fetal calf serum ...
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bioRxiv - Immunology 2021Quote: The mice lungs were obtained at 24 h postinjection with DiI-stained liposomes and digested with collagenase type I (Sigma, St. Louis, MO) to obtain single cells ...
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bioRxiv - Cancer Biology 2021Quote: ... fresh sterile samples from resected tumors were minced in Dulbecco’s modified Eagle medium (DMEM; Corning, cat # 10013CV and incubated with collagenase type I (3%; Sigma-Aldrich, cat # C-0130), DNase I (0.02% ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were rendered nucleotide free prior to mixing by incubating for 20 min with 0.2 U/ml apyrase (Type VII, Sigma Aldrich, St Louis, MO). Fluorescence enhancement of the MANT-fluorophore was monitored by energy transfer from vicinal tryptophans by exciting at 295 nm and monitoring 90° from the incident beam through a 450 nm broad bandpass filter (Omega Optical ...
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bioRxiv - Cell Biology 2022Quote: ... Ten grams medical grade 262 Bloom Type A gelatine (Gelita, Ter Apelkanaal, the Netherlands) was added to 1L 0.25M carbonate-bicarbonate (CB, Sigma-Aldrich, Zwijndrecht, the Netherlands) buffer pH 9.0 and allowed to dissolve at 50°C for 60 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues collected from leptomeninges and peri-infarct cortex are gently minced and then digested at 37°C for 30 min with an enzyme cocktail (Collagenase type IV; Sigma-Aldrich, C5138, DNase I; Sigma-Aldrich, D4263). FACS analysis was performed to determine tdTomato+ and CD271 FITC using BD Fortessa with a no labeled control for determining appropriate gates ...
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bioRxiv - Microbiology 2023Quote: C57BL/6J wild type and LDLR KO (B6.129S7-Ldlrtm1her/J) mice were intraperitoneally injected by 3% brewer thioglycollate medium (Millipore-Sigma, St. Louis, MO, USA). Mouse peritoneal cavity elicited macrophages (MØ ...
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bioRxiv - Bioengineering 2022Quote: ... and then incubated with the cell dissociation buffer composed of Eagle’s Minimum Essential Medium (EMEM) supplemented with a mixture of collagenase type XI and DNase (Sigma-Aldrich, St. Louis, MO) at a concentration of 250 mg/L and 50 mg/L ...
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bioRxiv - Biophysics 2024Quote: ... were prepared at the desired concentration in PBS containing Ca2+ and Mg2+ (PBS+/+) mixed with 30 µg/ml collagen type IV (Sigma-Aldrich, M7027-100G). Vitronectin (StemCell Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Hepatocytes were seeded on polystyrene tissue culture dishes of appropriate size pre-coated by collagen type I (Sigma, C3867, 6 – 10 mg/cm2). The homozygous mutant Wistar rat strain (Gunn-UGT1A1 ...
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bioRxiv - Cell Biology 2020Quote: ... blocking was performed in 3% BSA in PEM80 for 45 minutes and incubated overnight with a primary antibody against αTub (mouse IgG1, Sigma Aldrich, B-5-1-2, dilution 1:500). The cells were again washed with PBS (3×5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... or GAPDH (mouse monoclonal anti-GAPDH antibody, 1:5000, Thermo Fisher Scientific Cat# AM4300, for fibroblasts and rabbit monoclonal Sigma-Aldrich Cat# G9545, 1:10000 for LX2 cells). Appropriate HRP-conjugated secondary antibodies were used (1:10000 Promega) ...
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bioRxiv - Genetics 2020Quote: ... incubated for 3 hours at 25 °C with the secondary antibody (anti-rabbit IgG peroxidase 1:10000, Sigma-Aldrich; anti-mouse IgG peroxidase 1:10000, Sigma-Aldrich; in 5% milk, 1xTBS,0,1% Tween) and washed 3 times with 1xTBS/0.1% Tween ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were fixed with 3.7% paraformaldehyde and washed 3 times with PBS before and after incubation with mouse anti-FLAG M2 primary antibody (Sigma, diluted 1:2000 in PBS with 1% BSA) or HRP-conjugated anti-mouse IgG secondary antibody (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... incubated at room temperature with secondary antibodies coupled to horseradish peroxidase (1:20,000 Thermo Fisher goat-anti-rabbit, or 1:20,000 Millipore Sigma goat-anti-rat in 5% nonfat dry milk). Membranes were washed again in PBS containing 0.1% Tween 4 x 15 min ...
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bioRxiv - Biophysics 2019Quote: ... The following antibodies and respective secondary antibodies were used in the indicated dilutions: polyclonal FN or LN antibodies (Sigma-Aldrich, St ...
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bioRxiv - Cell Biology 2022Quote: ... After blocking in 5% BSA, membranes were incubated with Anti-FASN rabbit antibody (#3189, CST) antibody or mouse anti-tubulin antibody (Sigma). Protein was next incubated with HRP-conjugated secondary antibodies and was detected using chemiluminescence.
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bioRxiv - Molecular Biology 2022Quote: ... PLG binding was detected by adding an anti-PLG primary antibody raised in sheep (Acris Antibodies) followed by horseradish (HRP)-conjugated anti-sheep secondary antibody (Sigma). Primary and secondary antibodies were diluted 1:1000 and 1:2000 ...
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bioRxiv - Molecular Biology 2022Quote: ... the wells were incubated with an anti-PLG primary antibody raised in sheep (Acris Antibodies) followed by horseradish (HRP)-conjugated anti-sheep secondary antibody (Sigma). Primary and secondary antibodies were diluted 1:2000 and 1:4000 ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The primary antibodies used in immunofluorescence (IF) were rabbit anti-LvIRF antibody (Genecreate) and mouse anti-β-actin antibody (Sigma). The slides were washed with PBS six times and then incubated with diluted second antibodies of anti-rabbit IgG (H+L ...
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bioRxiv - Biophysics 2021Quote: ... The primary antibodies were mouse monoclonal anti–α-tubulin antibody (T5168, Sigma), or/and rabbit anti-Tom20 antibody (HPA011562 ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... GFP antibodies (50430-2-AP, Proteintech; MAB3580, Millipore; Antibody Facility MPI-CBG) 1:250 and Histone H3 (phospho S28 ...
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bioRxiv - Plant Biology 2019Quote: ... primary antibodies and secondary antibodies coupled to Horseradish Peroxidase (HRP, Sigma Aldrich) for protein detection on blots.
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bioRxiv - Immunology 2021Quote: ... Antibodies used in this study were: anti-FLAG antibody (F1804, Millipore SiGMa), anti-NEK7 (3057S ...
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bioRxiv - Microbiology 2022Quote: ... samples were treated with SP_1505 antibody or control rabbit IgG antibody (Sigma) at concentrations indicated in figure legends ...
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bioRxiv - Neuroscience 2020Quote: ... Validated antibodies were purchased as follows: anti-CRMP2 polyclonal antibody (Sigma-Aldrich Cat# C2993 Research Resource Identifier (RRID) ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibody for DENV was MAB8705 Anti-dengue virus complex antibody (Millipore); secondary antibody was the Goat anti-mouse Alexa Fluor 488 ...
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bioRxiv - Plant Biology 2019Quote: The antibody fractions of the polyclonal HRP antibody mixture (P7899, Sigma-Aldrich) binding to α1,3-fucose and anti β1,2-xylose ...
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bioRxiv - Biochemistry 2019Quote: ... The following antibodies were used: anti-FLAG M2 monoclonal Antibody (Sigma Aldrich), anti-Fibrillarin (Cell Signaling Technology).
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bioRxiv - Pathology 2021Quote: ... Antibodies used were a rabbit polyclonal antibody to podocin (Sigma-Aldrich, P0372) used at a dilution of 1:100 ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibody dilutions for Rabbit Anti Lamin A antibody (Sigma Aldrich L1293), Anti Lamin B (Santacruz sc-6217 ...
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bioRxiv - Plant Biology 2022Quote: ... Other primary antibodies we employed were: anti-HA tag antibody (Sigma, H6908); anti-c-Myc tag antibody (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used were anti-MNV VP1 monoclonal antibody (MABF2097, Sigma-Aldrich), anti-CD300lf monoclonal (MAB27881 ...
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bioRxiv - Physiology 2022Quote: ... The BCKDK antibody (AV52131) and AUH antibody (AV40374) were purchased from Sigma. Anti-PPARγ (#81B8) ...
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bioRxiv - Physiology 2023Quote: ... Primary antibodies used are Rabbit Anti-Tyrosine Hydroxylase Antibody (TH, Sigma AB152) and Goat Anti-Vesicular Acetylcholine Transporter Antibody (VAChT ...
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bioRxiv - Molecular Biology 2023Quote: ... except primary antibody incubation with γH2AX antibody (Millipore cat.no. 05-636,1:500) were performed at 4°C overnight.
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bioRxiv - Physiology 2024Quote: ... The primary antibody was incubated with Goat polyclonal antibody (AB758, Sigma-Aldrich) at 4°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... The primary antibodies were a mouse anti-FLAG antibody (F1804, Sigma-Aldrich), a rat anti-HA antibody (11867423001 ...
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bioRxiv - Neuroscience 2024Quote: ... the primary D1R antibody is a rat monoclonal antibody purchased from Sigma (cat ...
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bioRxiv - Cancer Biology 2021Quote: Paraffin sections of 6 archival primary ERMS tumours from KK Women’s and Children Hospital in Singapore were analysed by IHC using anti-MCU antibody (1:50, Sigma-Aldrich). Following Institutional Review Board approval (CIRB 2014/20179) ...