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Citations for Millipore Sigma :
651 - 700 of 7557 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... the disulfide bond linking the probe and the fluorophore was cleaved with Cleavage buffer comprising 2×SSC and 50 mM TCEP (Sigma, 646547) for 15 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... 50 nM readout probes in 10% EC Buffer (1 mL of ethylene carbonate, 7 mL Ultrapure RNase/DNase free water, 2 mL 20x SSC, and 1g Low MW dextran sulfate Sigma #D4911) was added to the cells and incubated for 20 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... An anti-bleaching buffer (50 mM Tris-HCl pH 8.0, 300 mM NaCl, 2×SSC, 3 mM trolox (Sigma-Aldrich, 238813), 0.8% D-(+)-Glucose (Nacalai Tesque ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% TritonX100 in 2 × SSC), anti-bleaching buffer (50 mM Tris-HCl pH 8, 300 mM NaCl, 3 mM Trolox (Sigma 238813), 0.8% D-glucose (Nacalai Tesque 168060-25) ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated in 20x SSC at 65 °C and stained for 20 min with 5% Giemsa solution (Sigma-Aldrich, GS500).
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bioRxiv - Cell Biology 2021Quote: Apoptotic cells were visualized with the terminal deoxynucleotidyl transferase (TdT)-FragEL DNA Fragmentation Detection Kit (Millipore, Etobicoke, ON, Canada) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... Ligation and amplification steps of the PLA were performed using the Duolink in situ Detection Reagents Orange kit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... using ortho-nitrophényl-β-galactoside as substrate (β-Galactosidase Reporter Gene Activity Detection Kit, Sigma St Louis MI USA).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed according to manufacturer’s instructions using ApopTag Fluorescein In Situ Apoptosis Detection Kit (Millipore).
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bioRxiv - Cancer Biology 2020Quote: ... Apoptotic cells were identified by TUNEL assay on sections using an ApopTag in situ apoptosis detection kit (Merck-Millipore).
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bioRxiv - Microbiology 2021Quote: Apoptotic cells in the lung were detected by immunohistochemistry using the ApopTag in situ apoptosis detection kit (EMD Millipore) following the manufacturer’s directions ...
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bioRxiv - Biochemistry 2021Quote: ... Pluripotency status of control and ABCF1-knockdown cells were analyzed using an alkaline phosphatase (AP) detection kit (EMD Millipore), or by RT-qPCR analysis of mRNAs purified using TRIzol reagent (Life Technologies).
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bioRxiv - Cancer Biology 2021Quote: ... TUNEL Assay detecting apoptotic cell death were performed with the ApopTag Fluorescein in situ apoptosis detection kit (S7110, Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: PLA experiments were done using Duolink™ II secondary antibodies and detection kits (Sigma-Aldrich, #DUO92002, #DUO92004, and #DUO92008) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2020Quote: ... Absence of mycoplasma in cell lines was routinely checked by the VenorGeM Mycoplasma Detection Kit (Sigma, St. Louis, MO).
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bioRxiv - Neuroscience 2023Quote: ... It was further processed for the detection of glutamate release using glutamate determination kit (Sigma Aldrich, USA, Cat# GLN1) as per manufacturer’s protocol.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cell lines were verified to be free of mycoplasma contamination by Venor™ GeM Mycoplasma Detection Kit from Sigma.
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bioRxiv - Molecular Biology 2023Quote: PLA experiments were done using Duolink™ II secondary antibodies and detection kits (Sigma-Aldrich, #DUO92002, #DUO92004, and #DUO92008) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... TUNEL staining was performed in a set of sections using the ApopTag in situ apoptosis detection kit (S7165, Millipore). Sections were visualized under confocal microscopy (Nikon) ...
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bioRxiv - Neuroscience 2024Quote: ... A fluorometric intracellular ROS detection kit was used to detect ROS levels at 30 and 120 minutes (Sigma, MAK144) using a Varioskan microplate reader (ThermoFisher).
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bioRxiv - Immunology 2023Quote: ... PLA probe maker kit and Duolink® in situ detection reagents were procured from Millipore Sigma (Burlington, MA, USA). RPMI medium 1640 was supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... All cell lines were certified to be free of mycoplasma contamination using the Venor GeM Mycoplasma Detection Kit (Sigma) prior to experimental data collection.
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bioRxiv - Cell Biology 2023Quote: ... TUNEL staining was made according to the manufacturer’s instructions of the apoptosis detection kit in situ red (S7165, Merck-Millipore).
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bioRxiv - Microbiology 2022Quote: ... The PCR products were purified using the kit Gene eluteTM Gel extraction kit (Sigma Aldrich, MA, USA), re-surveyed on Nanodrop® and delivered for Sanger DNA sequenced at Eurofins Genomics (Ebersberg ...
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bioRxiv - Developmental Biology 2021Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Millipore-Sigma) as described previously (1 ...
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bioRxiv - Neuroscience 2022Quote: Genotyping for the R1.40 transgene locus was done with the recommended PCR primers using the PCR Ready Mix kit (E3004; Sigma-Aldrich). The sequences of the genotyping primers were as follows ...
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bioRxiv - Genetics 2021Quote: ... Purification of genomic Polymerase Chain Reaction (PCR) amplified DNA was performed with the GenElute PCR Clean-Up kit (Sigma-Aldrich) or the GeneJET PCR Purification Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... or LIVE/DEAD™ Fixable Blue Cells Stain Kit (#L34957, Thermo; 1:1000 in PBS) and fixation for 15 minutes at RT using 1.85% formaldehyde (F1635, Sigma) in PBS solution before surface staining with antibodies in PBS supplemented with 0.5% BSA [fraction V ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity purified FH-Ago2 bound with miRNA (most population bound with miR-122 due to its overexpression) was eluted from anti-FLAG beads by competing with 3X FLAG peptide (Sigma) as per the manufacturer’s protocol in a purification buffer (30mM HEPES pH 7.4 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... was purified using the GeneElute PCR Clean-Up Kit (Sigma-Aldrich, USA), then labeled with biotin-16-dUTP (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Mice were genotyped using Extract-N-Amp Tissue PCR Kit (Sigma Aldrich) according to manufacturers’ instructions with the following primers ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we purified both libraries using the GenElute PCR Clean-Up Kit (Sigma) to remove short library fragments ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... Mice were genotyped using Extract-N-Amp Tissue PCR Kit (Sigma Aldrich) according to manufacturer instructions with the following primers ...
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bioRxiv - Physiology 2022Quote: ... and purified with the GenElute™ PCR Clean-Up Kit (SIGMA NA1020). 300 ng of the eluted DNA was digested overnight at 37°C with the restriction enzymes including USP8 – BstBI and USP48 – SpeI ...
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bioRxiv - Neuroscience 2023Quote: ... and PCR amplification was performed using KAPA2G ReadyMix Kit (Sigma Aldrich, KK5103) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: PCRs were performed with the KAPA3G Plant kit (Sigma Aldrich, MO, USA) using the following conditions ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by 3X washes with 2XSSC and 30 min incubation in 2X SSC + 0.1% Tween20 (2XSSCT) + 50% (v/v) formamide (EMD Millipore, cat#S4117). For each sample ...
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bioRxiv - Bioengineering 2021Quote: ... for 12 days in SSC expansion media with the following additions: 100 ng/mL follicle stimulating hormone from human pituitary (FSH, Millipore Sigma, F4021), 10 ng/mL luteinizing hormone from human pituitary (LH ...
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bioRxiv - Bioengineering 2022Quote: ... we first mixed together all the secondary probes with 10% hybridization buffer with Triton-X (10% formamide, 10% dextran sulfate, 2X SSC, and 0.1% Triton-X (Sigma-Aldrich, T8787-100ML)) to a final ~20nM concentration per probe (range ...
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bioRxiv - Physiology 2022Quote: ... Dead cells and doublets were excluded on the basis of FSC and SSC distribution and DAPI exclusion (Sigma-Aldrich, catalogue number D9542) exclusion ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by 3X washes with 2XSSC and 30 min incubation in 2X SSC + 0.1% Tween20 (2XSSCT) + 50% (v/v) formamide (EMD Millipore, cat#S4117). For each sample ...
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bioRxiv - Genomics 2022Quote: Samples were then co-stained with 5ng/ml DAPI for 15 mins and imaged in an anti-bleaching buffer (50mM Tris-HCl, pH=8.0, 300mM NaCl, 2×SSC, 3mM Trolox (Sigma-Aldrich, Cat. 238813), 0.8% D-glucose (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... 5x SSCT buffer was prepared by diluting 20x SSC (saline sodium citrate) in DEPC water and adding 0.1% tween (Sigma Aldrich, Cat# P1379). Next ...
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bioRxiv - Biophysics 2023Quote: ... cells in each well were incubated with 100 µL of hybridization buffer (2× SSC, 50% formamide and 10% w/v dextran sulfate (Sigma, D8906-50G) mixed with ∼1 µM total primary probes ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by 3X washes with 2XSSC and 30 min incubation in 2X SSC + 0.1% Tween20 (2XSSCT) + 50% (v/v) formamide (EMD Millipore, cat#S4117). For each sample ...
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bioRxiv - Cell Biology 2024Quote: ... cells were deproteinated for 15 minutes in 0.1 N HCl and neutralized for 5 minutes in 2X Saline Sodium Citrate buffer (2X SSC) (Sigma Aldrich, Cat # S6639) at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... for 1h at RT, blocked for 1h at RT in blocking solution (0.3% Triton X-100, 0.2% BSA (A4503, Sigma), and 5% goat serum (005-000-121 ...
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bioRxiv - Molecular Biology 2020Quote: ... with Duolink Detection Reagent Green (Sigma, DUO92014) or Orange (DUO92007) ...
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bioRxiv - Synthetic Biology 2020Quote: ... and visualized using chemiluminescence detection (Immobilon, Millipore). Coomassie brilliant blue staining was conducted using Bullet CBB Stain One Super (Nacalai ...
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bioRxiv - Cell Biology 2021Quote: ... Protein detection using anti-HA (H9658, Sigma) or anti-Scp160 antibodies (Gift of R ...