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Citations for Millipore Sigma :
651 - 700 of 10000+ citations for Recombinant Mouse Icam1 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 5% foetal calf serum (FCS; Sigma-Aldrich) for staining ...
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SIMON, an automated machine learning system reveals immune signatures of influenza vaccine responsesbioRxiv - Immunology 2019Quote: ... supplemented with 10% FCS and human serum AB (Sigma) for 2 days at 107 cells/ml in 24-well plate following “RESTORE” protocol (67 ...
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bioRxiv - Cell Biology 2019Quote: ... Media was also supplemented with 10% dialysed FCS (Sigma), penicillin (100 IU/mL) ...
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bioRxiv - Microbiology 2020Quote: ... Nanosota-1C-Fc was cloned into pET42b vector (Novagen) with a C-terminal human IgG1 Fc tag ...
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bioRxiv - Neuroscience 2019Quote: ... supplemented with 10% Foetal Calf Serum (FCS, Sigma-Aldrich), 50 U/ml penicillin (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: hACE2-Fc was digested with thrombin protease (Sigma Aldrich) in the presence of 2.5 mM CaCl2 at a 1:300 w/w thrombin:protein ratio ...
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bioRxiv - Microbiology 2021Quote: ... and 5% FCS (Sigma-Aldrich, St. Louis, MO, USA) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... and/or 2.5 μg/ml E-cadherin-Fc (Sigma). IELs were plated in RPMI-T medium containing 2 μg/ml PerCP-e710 conjugated anti-CD107a (1D4B ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% fetal calf serum (FCS, Sigma Aldrich), 100 μg/ml streptomycin and 100 U/ml penicillin (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... goat anti-human IgG (Fc) peroxidase conjugate (Sigma A0170) for 45 min ...
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bioRxiv - Immunology 2021Quote: ... using an anti-human Fc mAb (Sigma-Aldrich, I6260) as capture ...
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bioRxiv - Immunology 2022Quote: ... and 10% FCS (Sigma-Aldrich, St. Louis, Missouri, USA)) assuming that they double within 24 hours ...
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bioRxiv - Immunology 2023Quote: ... mixed in 2% fetal calf serum (FCS; Sigma-Aldrich). Cells were stained with combinations of the following fluorophore-conjugated anti-mouse monoclonal antibodies ...
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bioRxiv - Immunology 2023Quote: ... 10% fetal calf serum (FCS) treated with Chelex (Sigma) to removed endogenous calcium ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma-Aldrich) and 2 mM L-glutamine (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma-Aldrich), 10 mM HEPES (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... and 1% FCS (Sigma Aldrich, St. Louis, MO, USA). Thereafter ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The cell suspension was incubated with 10% FCS (Sigma) for 30 min to allow the cell membrane to recover from trypsinisation and prevent false positive staining with Annexin V (AV) ...
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bioRxiv - Cancer Biology 2023Quote: ... 10% tetracycline-free fetal calf serum (FCS) (Sigma-Aldrich), 100 U/ml penicillin (Biochrom) ...
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bioRxiv - Genomics 2024Quote: ... containing 10% FCS (heat inactivated) (Sigma-Aldrich, Cat# F7524) and 0.5% penicillin and streptomycin at 27°C.
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% FCS (v/v) (Sigma- Aldrich Ltd), apart from T2 cells which were grown in glutamine containing RPMI (Sigma-Aldrich Ltd) ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma Aldrich). All cells were kept at 37 °C with 5% CO2 and tested for mycoplasma at regular intervals.
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% heat-inactivated FCS (Sigma Aldrich #F2442) and 50 ng/ml M-CSF (PeproTech #300-25 ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% fetal bovine serum (FCS, Sigma-Aldrich), 100 U/ml penicillin (ThermoFisher) ...
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bioRxiv - Plant Biology 2021Quote: The inhibitory effect of recombinant P235 on recombinant glycosyl hydrolase was assayed using the β-Glucosidase Activity Assay Kit (MAK129, Sigma). Enzymatic reactions were carried out in K-Phosphate buffer (100 mM ...
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iDePP: a genetically encoded system for the inducible depletion of PI(4,5)P2 in Arabidopsis thalianabioRxiv - Plant Biology 2020Quote: ... 2 ml of 50 % HIS-Select® Cobalt affinity resin (Sigma) was washed with milli-Q water and then with equilibration buffer (20 mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... The cell lysate was combined with His-Select Co2+ resin (Sigma) for 1 hour at 4 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... complete His-Tag Purification Resin (NiNTA) were purchased from Sigma Aldrich. Protein inhibitor cocktail (PIC ...
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bioRxiv - Biochemistry 2021Quote: ... anti-TtSmtB antibodies (GeneCust™) and His-tag antibodies (Sigma-Aldrich). His-tag removal from recombinant TtSmtB was performed as previously described (22).
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 800 mM) ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 300 mM) ...
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bioRxiv - Microbiology 2021Quote: ... and a combination of α-6×His antibody (Sigma Aldrich, H1029) and HisProbe-HRP (Thermo ...
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bioRxiv - Cell Biology 2021Quote: ... His-tag was removed using an Enterokinase Cleavage Capture kit (Novagen), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... the proteins were purified using His-Select Nickel agarose resin (Sigma) and anion exchange chromatography (Source Q ...
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bioRxiv - Microbiology 2021Quote: ... His-tag removal was carried out by overnight HRV-3C (Sigma) digestion at 4°C and the cleaved protein was then purified on Ni-NTA before being subjected to SEC on Superose 6 10/300 column (Cytiva ...
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bioRxiv - Cell Biology 2021Quote: His-ACBD5 was expressed in BL21 Rosetta (DE3) cells (EMD Millipore) induced with 1 mM IPTG overnight at 18°C ...
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bioRxiv - Biochemistry 2019Quote: ... The His-tag was cleaved by digestion with thrombin (Sigma-Aldrich) in the presence of 2 mM CaCl2 at 37°C for 2 hours ...
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bioRxiv - Microbiology 2021Quote: ... Ni2+-NTA His-bind slurry was obtained from Novagen (Darmstadt, Germany). Rhodamine B isothiocyanate-Dextran (RITC-Dextran ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins were purified using Ni2+-containing His-Bind columns (Novagen). Purified proteins were desalted and concentrated by ultracentrifugation (Amicon ...
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bioRxiv - Cell Biology 2020Quote: ... followed by immunoblotting with HRP-conjugated anti-His antibody (Sigma-Aldrich) by using SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... –Ade (20 ug/mL L-His HCl monohydrate (A-9126, Sigma) added to –Trp ...
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bioRxiv - Microbiology 2021Quote: ... probed using 1:500 monoclonal anti-His HRP-conjugated antibodies (Sigma) and imaged using a ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Genetics 2022Quote: ... the ORF of eggpl was inserted into vector piztv5-His (Novagen) in our laborary to overexpress eggpl ...
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bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-His primary antibodies (dilution 1:3000, #H1029 Sigma Aldrich).
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bioRxiv - Biochemistry 2023Quote: ... and supernatant was bound to Ni-NTA His-Bind Resin (Novagen) for 1hr at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was loaded onto Ni-resin (HIS-Select, Sigma-Aldrich) and washed with buffer 1 (50 mM Hepes-KOH pH7.6 ...
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bioRxiv - Developmental Biology 2021Quote: ... Flag-tagged proteins were purified by immunoprecipitation with anti-Flag M2 antibody (Sigma) and Protein G Mag Sepharose (GE healthcare) ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins associated with 3xFLAG-tagged MBD3 were purified using anti-FLAG sepharose (Sigma) and processed for mass spectrometry as described (Smits et al. ...