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Citations for Millipore Sigma :
551 - 600 of 10000+ citations for Recombinant Mouse Icam1 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The cells were maintained in DMEM supplemented with 10% FCS for three days and then incubated with DMEM supplemented with 10% FCS and 5 μg/ml puromycin (Sigma-Aldrich) for four days to allow the selection of cells expressing the gene of interest (40).
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bioRxiv - Immunology 2023Quote: ... harvested neutrophils were resupended in PBS + 2% FCS and Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). Cells were stained with CD15-APCCy7 (1:100 ...
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bioRxiv - Immunology 2023Quote: ... harvested neutrophils were resupended in PBS containing 2% FCS and Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). Cells were stained with the following antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... ephrinB1-Fc (R&D, #473-EB) or ephrinB2-Fc (R&D, #496-EB) were pre-clustered using goat anti-human Fc IgG (Sigma, #I2136) in 4:1 ratio for 30min.
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bioRxiv - Biochemistry 2023Quote: ... The cells were cultured and passaged every 2 or 3 days in Dulbecco’s modified Eagle’s medium (DMEM) (Hi Media Laboratories) supplemented with 10% FBS (Hi Media Laboratories) and 1% penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA). Both cell types were cultured at 37°C and in a humidified incubator with a 5% CO2 atmosphere ...
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bioRxiv - Bioengineering 2019Quote: The His-TEV tag was cleaved using TEV protease (Sigma). The reaction was made overnight at 4°C into dialysis buffer for TEV (25 mM Tris-HCl ...
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bioRxiv - Plant Biology 2019Quote: ... HIS-TAN1-GFP was conjugated with Atto488-maleimide (Sigma 28562). 4μM HIS-TAN1-GFP in 80mM PIPES ...
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bioRxiv - Immunology 2021Quote: ... This was followed by 0.5ug/mL anti-his HRP (Sigma), and binding was detected after incubation with substrate at an absorbance of 450nm ...
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bioRxiv - Biochemistry 2020Quote: ... The antibodies used for western blotting were anti-His (Sigma), anti-Strep HRP conjugated (iba ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG tag (F1804) or His tag (H1029) were from Sigma. Mouse monoclonal antibody raised against hemagglutinin (HA ...
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bioRxiv - Plant Biology 2020Quote: ... and 1:5000 dilution of primary anti-His (SIGMA, USA), 1:10000 for anti-GST (SIGMA ...
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bioRxiv - Molecular Biology 2021Quote: ... SD-Leu-Trp-His+1mM 3-AT (3-aminotriazol, Sigma) and SD-Leu-Trp-His-Ade (Formedium).
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bioRxiv - Genetics 2021Quote: ... His-TCF7 was PCR cloned into pET52b (Millipore, 71554-3) using the primers 5’-CGGGGTACCTCATGCCGCAGCTGGACTCC-3’ and 5’-ATAGTTTAGCGGCCGCGAGCACTGTCATCGGAAGGAAC-3’ ...
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bioRxiv - Biochemistry 2021Quote: α-PCNA (P8825) and α-HIS (H1029) were purchased from Sigma; α-Cul2 (EPR3104 ...
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bioRxiv - Genetics 2022Quote: ... and detected with His and GST antibodies (Sigma-Aldrich, USA), respectively.
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bioRxiv - Synthetic Biology 2023Quote: ... Anti-His primary antibody (H1029) was purchased from Sigma Aldrich, HRP conjugated secondary antibody (J1430 ...
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bioRxiv - Cancer Biology 2023Quote: ... which were cryopreserved in HI FBS with 10% DMSO (Sigma). Cells were directly transferred to a -80 °C freezer in Coolcell freezing containers (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... 2% heat-inactivated Fetal Bovine Serum (HI-FBS; Sigma-Aldrich), 1% v/v penicillin-Streptomycin (VWR) ...
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bioRxiv - Biochemistry 2023Quote: ... and BRAF NTs/ BRAF KD with His antibody (Sigma SAB5600227). Finally ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% HI-horse serum and vancomycin (Sigma, USA). The inoculated flask was then kept in shaking conditions (100 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... One milliliter of equilibrated HIS-Select Nickel Affinity Gel (Sigma) was added to the cleared cell lysate and incubated with mild agitation for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3% heat-inactivated fetal bovine serum (HI-FBS; Sigma) at 37°C for 15 ∼ 30 min ...
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bioRxiv - Microbiology 2024Quote: ... and heat-inactivated serum (HI-FBS; Sigma F9665, Lot# BCCB5091) was aliquoted and stored at −20°C until required.
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bioRxiv - Immunology 2023Quote: ... 21 and 42 with 50 μg of each of five recombinant LMWs in alum mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, #70323-44-3). ELISA was performed to measure serum responses to antigen (see methods below ...
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bioRxiv - Molecular Biology 2020Quote: ... and FLAG-tagged ribosomes were purified using M2 FLAG-affinity resin (Sigma), as described previously (Inada et al. ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged proteins were eluted by competition using the FLAG peptide (Sigma).
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bioRxiv - Neuroscience 2019Quote: ... Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma). Immunoprecipitates were incubated for at least 2 h at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... FLAG-tagged TSC2 was immunoprecipitated with ANTI-FLAG® agarose beads (Sigma) for 2 hours at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... HA-tagged proteins were recognized using a murine antiHA antibody (Sigma-Aldrich) and then an antiMouse IgG-peroxydase antibody (KPL) ...
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bioRxiv - Molecular Biology 2020Quote: ... TAP tagged proteins was captured using IgG sepharose fast flow beads (Sigma) and proceeded as described (33) ...
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bioRxiv - Biochemistry 2019Quote: ... Strep-II-tagged proteins were bound to Strep-Tactin resin (EMD Millipore), washed with lysis buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-PAP 1:1000 (for detecting TAP-tagged proteins) (Sigma-Aldrich, P1291), anti-Rad53 1:2000 (abcam ab104232) ...
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bioRxiv - Molecular Biology 2023Quote: ... Flag-tagged ZC3H11A was immunopurified with anti-Flag(M2) magnetic beads (Sigma) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... or control plasmid expressing 3xFLAG-tagged TRIM25 through X-tremeGENE9 (Millipore Sigma). Twenty-four hours later ...
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bioRxiv - Cell Biology 2023Quote: ... or Glutathione High Capacity Magnetic Agarode Beads (for GST-tagged proteins, SIGMA). After 2 extensive washing with binding buffer ...
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bioRxiv - Microbiology 2023Quote: ... FLAG-tagged protein signals were detected with anti-FLAG (Sigma-Aldrich, F1804) in combination with anti-mouse secondary antibody (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... FLAG-tagged proteins were immunoprecipitated with FLAG-M2 antibody (F1804 Sigma-Aldrich) conjugated to protein G dynabeads (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... After incubation with peroxidase-tagged secondary antibodies (1:2000 diluted, Sigma-Aldrich), membranes were revealed with an ECL-plus chemiluminescence western blot kit (Amershan-Pharmacia Biotech).
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... followed by HRP-tagged anti-rabbit secondary antibody (1:10,000, Sigma-Aldrich).
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bioRxiv - Bioengineering 2021Quote: ... The binding of VHH-Fcs/ACE2-Fc to S1 was detected using 100 µL 1 µg/mL HRP-conjugated goat anti-human IgG (Sigma, Cat#A0170). Finally ...
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bioRxiv - Bioengineering 2021Quote: ... plates were washed 10 times with PBST and the ACE2-Fc binding was detected using 1 µg/mL goat anti-human IgG (Fc specific) HRP conjugate antibody (Sigma, Cat#A0170) in 100 µL PBSCT ...
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bioRxiv - Cell Biology 2023Quote: ... human ephrin-B2-Fc (10 µg/ml, RnD Systems, #7397-EB) was mixed with Cy3-conjucated Fc-antibody (5 µg/ml, Sigma-Aldrich, # C2571) in HBSS and incubated for 30-60 minutes at room temperature with gentle agitation ...
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bioRxiv - Molecular Biology 2020Quote: ... and 10 μg/ml recombinant human insulin (Sigma). BT474 cells were cultured in RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix) ...
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bioRxiv - Immunology 2022Quote: ... and 0.5% recombinant human serum albumin (Sigma Aldrich). Microfluidic droplets were generated as water-in-oil emulsions using a co-flow of aqueous phases ...
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bioRxiv - Developmental Biology 2019Quote: ... and 1000 U/ml recombinant LIF (ESGRO, Millipore). 2i Medium (N2B27 ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant mTOR was purchased from Millipore (14-770) and recombinant 4EBP1 was purchased from Millipore (516678) ...
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bioRxiv - Biochemistry 2020Quote: ... 10 μg/mL human recombinant insulin (Sigma Aldrich), 100 μM AAP ...
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bioRxiv - Biochemistry 2019Quote: Recombinant human insulin was purchased from Sigma-Aldrich (Cat# I2643 ...
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bioRxiv - Microbiology 2019Quote: ... and incubated with recombinant protein (GenScript, Sigma, Tocris) of each analyte for 4 h at room temperature ...