Labshake search
Citations for Millipore Sigma :
651 - 700 of 10000+ citations for Creatinine Serum Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... membranes were incubated with 5% skimmed milk for 1 hour at room temperature and then with primary antibodies diluted in 2% bovine serum albumin (Sigma-Aldrich) in tris-buffered saline 0.05% Tween 20 (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2021Quote: Samples were diluted 1:2 in serum matrix for analysis with Milliplex Human Cytokine/Chemokine Magnetic Bead Panel as per manufacturer’s instructions (EMD Millipore Corporation). Concentrations for analytes (EGF ...
-
bioRxiv - Immunology 2021Quote: ... virus was diluted in ½ log10 in minimum essential medium (MEM) supplemented with 2% aseptically filtered bovine serum album (BSA) solution (Millipore-Sigma). Serially diluted virus was added to Vero E6 cells (2X104 cells/well in MEM with 2% BSA ...
-
bioRxiv - Immunology 2022Quote: ... and mesenteric) (n=3) were pooled in digestion medium consisting of RPMI 1640 with 2% fetal bovine serum (FBS) (Sigma-Aldrich), deoxyribonuclease (DNase ...
-
bioRxiv - Immunology 2022Quote: ... For immunohistochemical analysis of bronchiolar epithelium paraffin sections were first blocked with 5% goat serum and 2% fish gelatin (Both from Sigma-Aldrich) at 25 0C for 45 mins ...
-
bioRxiv - Bioengineering 2020Quote: ... Plates were washed with phosphate-buffered saline with 0.1% Tween-20 (PBST) four times and then blocked with 2% bovine serum albumin (BSA, Sigma, #B2064-50G) in PBST for 2 hours at room temperature ...
-
bioRxiv - Bioengineering 2020Quote: ... Plates were washed with phosphate-buffered saline with 0.1% Tween-20 (PBST) four times and then blocked with 2% bovine serum albumin (BSA, Sigma, #B2064-50G) in PBST for 2 hours at room temperature ...
-
bioRxiv - Genetics 2022Quote: ... Bb was cultured at 37ºC in Barbour-Stonner-Kelly with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer media (BSK-H) complete with 6% rabbit serum (Millipore Sigma) and 1% Amphotericin B (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells from each donor animal were plated as separate cultures in serum-free growth medium42 (SFGM, Supplementary Table 3) on fibronectin-coated (4 μg cm−2 bovine fibronectin; F1141, Sigma-Aldrich) tissue culture vessels for 72 h prior to FACS ...
-
bioRxiv - Biophysics 2019Quote: ... and 1 mM Tris-2-carboxyethyl-phosphine) containing ATP-regeneration and oxygen-scavenging systems as well as 1 mg/mL bovine serum albumin (Sigma-Aldrich). The beads were trapped by two 1064-nm lasers in a passive-force clamp mode ...
-
bioRxiv - Neuroscience 2019Quote: ... Dissociated DRG neurons were plated onto plastic coverslips in 24-well plates after washing and grown in culture medium (MEM in 5% fetal bovine serum, 20 μM 5-fluoro-2-deoxyuridine, 20 μM uridine, 100 units /ml penicillin and 100 ng/ml streptomycin, Sigma Aldrich) for 3 days at 37°C in a CO2-humidified incubator ...
-
bioRxiv - Bioengineering 2020Quote: ... blocked with 10% bovine serum (Sera plus) for 30 min and stained with Phalloidin-TRITC (2 µg/ml, P1951, Sigma Aldrich) for 45 min at room temperature ...
-
bioRxiv - Cancer Biology 2020Quote: Non-synchronized and synchronized cells were fixed using 70% Ethanol for 48 hours at 4°C then washed with PBS+2% goat serum (Sigma, UK) and resuspended in 1mL of ice-cold PBS ...
-
bioRxiv - Cancer Biology 2020Quote: ... All other cell lines were grown in Dulbecco`s modified Eagle medium (DMEM) (Fisher, UK) supplemented with 2% human serum (Sigma, UK) and maintained in an humidified sterile incubator with 5% CO2 at 37°C.
-
bioRxiv - Synthetic Biology 2020Quote: ... after which the medium containing the transfection reagent and DNA mixture was replaced with 2 mL of complete medium supplemented with 10% fetal bovine serum (FBS; Sigma-Aldrich). Transfected cells were cultured 16 h before experiments.
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... for 15 minutes at −20°C and washed again with PBS after which aspecific antibody-binding was blocked for 2 hours in 5% bovine serum albumin (BSA, Sigma-Aldrich). Organoids were incubated overnight at 4°C with the primary antibodies (mouse anti-acetylated α-tubulin (Santa Cruz ...
-
bioRxiv - Developmental Biology 2020Quote: ... After 4 days, cells were switched to DMEM/F-12, HEPES (31330038, Thermofischer Scientific) with 2% Horse serum (H1270, Sigma-Aldrich). Before staining ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were washed twice in 0.1M PBS and blocked for 2 hours with 0.1M PBS containing 1% bovine serum albumin and Triton X-100 (0.1%) (both from Sigma Aldrich, Gilligham, UK) and then incubated for 48 hours at 4°C with the primary antibody ...
-
bioRxiv - Biophysics 2020Quote: ... the growth medium was exchanged to differentiation medium consisting of DMEM supplemented with 2 % horse serum (HS, Sigma, St. Louis, USA), 1 % penicillin-streptomycin (Gibco ...
-
Splicing variation of BMP2K balances endocytosis, COPII trafficking and autophagy in erythroid cellsbioRxiv - Cell Biology 2020Quote: ... All media had high glucose concentration and were supplemented with 10% fetal bovine serum and 2 mM L-glutamine (Sigma-Aldrich). Hemin (H9039 ...
-
bioRxiv - Microbiology 2020Quote: ... was maintained in MEM (Minimal Essential Medium) containing 10% (v/v) foetal bovine serum,10,000 U penicillin/streptomycin and 2% (v/v) L-glutamine (Sigma Aldrich, Germany). Both SARS-CoV-2/FFM1 and SARS-CoV-2/FFM7 were isolated as previously described (Hoehl et al. ...
-
bioRxiv - Neuroscience 2020Quote: ... and blocked with 2% donkey serum and then immunostained using rabbit antibody against Cx43 (Figure 6A, Sigma, cat# C6219, 1:500) or Alpaca antibody against Green Fluorescent Protein conjugated to Alexa Fluor 488 (also labels EBFP2 ...
-
bioRxiv - Immunology 2020Quote: ... the blockade of unspecific epitopes was achieved by a 60-minute incubation in a solution comprised of 2% bovine serum albumin (Sigma Aldrich) (w/v) ...
-
bioRxiv - Genomics 2021Quote: ... the supernatant was removed by aspiration and the nuclear pellet was resuspended in 200 uL staining mix (2% normal goat serum, 0.1% BSA, 1:500 anti-NeuN conjugated to AlexaFluor488 (Millipore, cat#: MAB377X) in PBS ...
-
bioRxiv - Microbiology 2021Quote: ... supplemented with 0.5 % v/v StemSure 10 mmol/l 2-mercaptoethanol solution (Wako) and 10% v/v fetal bovine serum (#F7524; Sigma-Aldrich), as the cell medium ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Single-cell suspensions were derived by mechanical disruption of bone marrow in PBS supplemented with 2% Fetal Calf Serum (FCS, Sigma, C8056). For FACS analysis ...
-
bioRxiv - Microbiology 2022Quote: ... and blocked with 2% horse serum in 1X Tris-buffered saline (20mM Tris, 150mM NaCl) containing 0.01% Tween® 20 detergent (Sigma-Aldrich) (TBS-T ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... NALM6 cells were cultivated in RPMI 1640 medium (Biowest) supplemented with 10% fetal bovine serum (FBS) (Biochrom AG) and 2 mM glutamine (Sigma-Aldrich). β2M-/- ...
-
bioRxiv - Neuroscience 2023Quote: ... the mixture was transferred into 15 ml centrifuge tube and 2 ml 1% bovine serum albumin (BSA; Sigma, USA, cat# A4628) was added to terminate dyeing ...
-
bioRxiv - Cell Biology 2022Quote: ... bone marrows were harvested by flushing mouse femurs and tibiae with ice-cold Sort Buffer (5mM EDTA, 25mM HEPES, 2% fetal bovine serum (FBS, SIGMA, #F2442) in 1× PBS (GIBCO ...
-
bioRxiv - Immunology 2023Quote: ... harvested neutrophils were resupended in PBS + 2% FCS and Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). Cells were stained with CD15-APCCy7 (1:100 ...
-
bioRxiv - Microbiology 2023Quote: ... albicans yeast at the ratio of 1:20 in RPMI 1640 medium containing 2% heat-inactivated mouse serum (#S3509, Sigma-Aldrich) at 37°C for 3 h.
-
bioRxiv - Immunology 2023Quote: ... harvested neutrophils were resupended in PBS containing 2% FCS and Fc receptors were blocked with 10% normal human AB serum (Sigma-Aldrich). Cells were stained with the following antibodies ...
-
bioRxiv - Neuroscience 2023Quote: ... samples were treated for 2 h at room temperature with 3% bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, MO, USA), and 0.3% PBS-T as a blocking agent ...
-
bioRxiv - Bioengineering 2023Quote: ... Blocking was done for 30 minutes at room temperature in AbDil or TBS-T + 2 w/v% Bovine serum albumin (BSA, Sigma Aldrich) for 2D and in TBS-T + 5 w/v% BSA for 3D ...
-
bioRxiv - Cancer Biology 2022Quote: ... 12-well non-tissue culture treated plates were coated with 32 μg/mL retronectin (WEHI) in PBS solution overnight at 4°C then blocked with 2% bovine serum albumin solution (#A1595 Sigma-Aldrich) in PBS at 37°C for 30 min were prepared ...
-
bioRxiv - Microbiology 2023Quote: ... supplemented with 10% (BC-1, BCBL-1) or 20% (BC-2, BC-3, BC-5, BJAB) Serum Plus-II (Sigma-Aldrich, catalog number 14009C ...
-
bioRxiv - Immunology 2023Quote: ... 5 x 105 MDCK cells per ml were prepared in serum free DMEM containing 2% v/v penicillin/streptomycin (Sigma-Aldrich) and 2 µg/ml TPCK Trypsin (Sigma) ...
-
bioRxiv - Bioengineering 2023Quote: ... Cells were plated in serum-free growth medium (SFGM, Supplementary Table 1) on fibronectin-coated (4 μg cm-2 bovine fibronectin; F1141, Sigma-Aldrich) tissue culture vessels ...
-
bioRxiv - Cell Biology 2023Quote: ... aeruginosa was diluted 20-fold into 2 mL of serum-free Eagle’s Minimum Essential Medium (EMEM) (Millipore Sigma, St. Louis, MO) in a 6-well plate ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were plated in serum-free growth medium (SFGM, Supplementary Table 1) on fibronectin-coated (4 μg cm-2 bovine fibronectin; F1141, Sigma-Aldrich) tissue cultureware.
-
bioRxiv - Biochemistry 2023Quote: ... then incubated for 1-2 h at room temperature in PBST with blocking agent (5 mg/mL Bovine Serum Albumin (Sigma Aldrich) for anti-Strep blots ...
-
bioRxiv - Neuroscience 2023Quote: ... Coverslips were then blocked and permeabilized with 2% donkey serum in DPBS Ca2+/Mg2+ supplemented with 0.1% Triton-X (Sigma, #93443-100ML) (blocking buffer) ...
-
bioRxiv - Neuroscience 2024Quote: ... The tissues were mashed through a 70-μm cell strainer in PBS / 2% heat-inactivated fetal bovine serum (HI-FBS; Sigma-Aldrich) / 10 mM EDTA-Na (pH 8.0) ...
-
bioRxiv - Cell Biology 2024Quote: ... a complex of siRNA and DharmaFECT 2 was prepared in serum-free DMEM (Sigma-Aldrich, St. Louis, MO, USA, Cat# D5796) at a volume of 100 μL/well according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... the cells were harvested and enzymatically dissociated into a single-cell suspension in PBS supplemented with 3% fetal bovine serum (#FB-1101, Biosera) and 2 mM EDTA (#ED2SS, Sigma=:Aldrich). After 15 minutes of incubation with 5 nM SYTOX™ Red Dead Cell Stain (#S34859 ...
-
bioRxiv - Cell Biology 2023Quote: ... Lipodisq® Styrene:Maleic Anhydride Copolymer 2:1 (Kit (Sigma-Aldrich, MO, USA) was used for the detergent-free membrane fraction purification ...
-
bioRxiv - Cancer Biology 2024Quote: ... This was after 2 washes in Buffer A (Sigma DUOlink PLA kit) and then incubated with the PLA kit Ligation solution (1X ligation buffer ...
-
bioRxiv - Microbiology 2019Quote: ... referred to here as hCMEC were obtained from Millipore and were maintained in an EndoGRO-MV complete medium kit supplemented with 1 ng/ml fibroblast growth factor-2 (FGF-2; Millipore) (81–84) ...
-
bioRxiv - Biochemistry 2021Quote: Protein concentrations were measured with the Bio-Rad Protein Assay Kit using bovine serum albumin (Sigma-Aldrich) as reference.