Labshake search
Citations for Millipore Sigma :
551 - 600 of 10000+ citations for Creatinine Serum Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... NRCMs with serum-free DMEM no glucose media and ARCMs with serum-free media 199 for 16 h before treating with 4-methyl 2-oxopentanoic acid sodium salt (ketoleucine, Sigma), sodium-3-methyl-2-oxobutyrate (ketovaline ...
-
bioRxiv - Cell Biology 2020Quote: ... All reactions were performed in the presence and absence of inosine monophosphate (IMP, 2 mM) in serum-free DMEM (Sigma) supplemented with 50 mM LiCl (Sigma) ...
-
bioRxiv - Developmental Biology 2021Quote: ... laevis were primed 2-14 days before ovulation by subcutaneous injection of 50 U pregnant mare serum gonadotropin (PMSG; Sigma) at the dorsal lymph sac ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were washed briefly in PBS and then blocked for 2 hours at room temperature in 10% normal donkey serum (Millipore) in 0.01M PBS containing 0.4% Triton X-100 ...
-
bioRxiv - Neuroscience 2019Quote: ... Following permeabilization cells were blocked for 1h using 2% bovine serum albumin (BSA) in PBST (PBS containing 0.05% tween 20; Sigma, P2287). Primary antibody against hKir 4.1 (1:100 ...
-
bioRxiv - Immunology 2019Quote: ... This was followed by incubation for 1 hour at room temperature in blocking buffer consisting of 2% goat serum (Sigma) in PBS 0.1% Triton X-100 ...
-
bioRxiv - Cell Biology 2020Quote: ... pre-washed three times with ice-cold NETN250 buffer and blocked for 3 h at 4°C on a rotating wheel with 500 µL ice-cold NETN250 buffer supplemented with 2 mg/mL bovine serum albumin (BSA, Sigma). After 3 h protein binding at 4 °C on a rotating wheel ...
-
bioRxiv - Microbiology 2019Quote: ... and then cultured for 24 h in serum-free media containing the amounts reported in Results of the glycolysis inhibitor 2-deoxy-D-glucose (2DG; D3179, Sigma), the glutaminase inhibitors BPTES (314045 ...
-
bioRxiv - Bioengineering 2019Quote: ... they were washed again 2 times with the wash buffer and incubated with 5% v/v goat serum (Sigma Aldrich) for 1 h ...
-
bioRxiv - Molecular Biology 2019Quote: In vitro BBB models were constructed and 1ml of serum-free EBM-2 medium containing 10 μg/mL HRP (0.5 mmol/L, Sigma-Aldrich) culture medium was added into the upper champer of the transwell system ...
-
bioRxiv - Animal Behavior and Cognition 2019Quote: ... was dissolved in saline (pH = 7.34) and FGF2 (20 μg/kg, i.p.; 2 mL/kg) was dissolved in 0.1% bovine serum albumin (Sigma Aldrich, Inc.) in 0.1 M phosphate-buffered saline (pH = 7.34) ...
-
bioRxiv - Immunology 2021Quote: ... the following components were added: 2 mM L-Glutamine (Fisher), 10% heat-inactivated (56°C, 60 min) human AB serum (Sigma), 12.5 mM HEPES (Fisher) ...
-
bioRxiv - Immunology 2021Quote: ... virus was diluted in ½ log10 in minimum essential medium (MEM) supplemented with 2% aseptically filtered bovine serum album (BSA) solution (Millipore-Sigma). Serially diluted virus was added to Vero E6 cells (2X104 cells/well in MEM with 2% BSA ...
-
bioRxiv - Biochemistry 2021Quote: ... For experiments involving C2C12 muscle cell differentiation, cells were washed with PBS and then incubated in differentiation media (DMEM, 2% horse serum, 50 nM insulin (Sigma)) for the indicated times ...
-
bioRxiv - Biochemistry 2020Quote: ... the medium was replaced with a serum-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days in the BDNF-containing media ...
-
bioRxiv - Biochemistry 2020Quote: ... Next the medium was replaced with a serum-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days in the BDNF-containing media ...
-
bioRxiv - Neuroscience 2020Quote: ... a 1:1 mixture of 4% HMS (2% final concentration) and 20% bovine serum albumin (BSA; 10% final concentration; V900933, Sigma), incubated at 4°C for 1 week ...
-
bioRxiv - Neuroscience 2019Quote: ... Cells were washed again as described above and blocked in buffer (2% (w/v) bovine serum albumin (BSA; Sigma-Aldrich)/PBS ...
-
bioRxiv - Cell Biology 2021Quote: ... myoblasts were differentiated into myotubes in DMEM supplemented with 2% (v/v) of horse serum (Sigma-Aldrich, Cambridgeshire, UK, H1270), 1% (v/v ...
-
bioRxiv - Cell Biology 2021Quote: ... which were diluted in the fluorescence dilution buffer (PBS supplemented with 5% FBS and 2% bovine serum albumin) containing 0.1% saponin (Sigma-Aldrich). After extensive washing with PBS ...
-
bioRxiv - Neuroscience 2020Quote: ... blocked in normal donkey serum in PBST (2%) and transferred to rabbit anti-Fos primary antibody (1:10000; EMD Millipore) in PBST and normal donkey serum for 16 hr at RT ...
-
bioRxiv - Microbiology 2021Quote: ... in serum-free media supplemented with 0,1 μg/ml L-1-p-Tosylamino-2-phenylethyl chloromethylketone (TPCK)-treated trypsin (Sigma-Aldrich). The supernatant was harvested at 72 h post infection when cytopathic effects were observed (with around 50% cell death) ...
-
bioRxiv - Biophysics 2020Quote: ... both paraformaldehyde and methanol fixed cells are incubated with primary antibody diluted in the antibody dilution buffer (PBS supplemented with 5% FBS, 2% bovine serum albumin and 0.1% saponin (Sigma-Aldrich)) ...
-
bioRxiv - Microbiology 2021Quote: Test sera were heat-inactivated at 56°C for 30 minutes to destroy any complement activity and serially diluted 1:2 in cell culture media containing 1% foetal calf serum (Sigma). Virus was diluted to give 100-250 foci in the virus-only control and then added to the serum dilutions before incubation for 1 h at 37°C ...
-
bioRxiv - Neuroscience 2020Quote: ... washed 3 times with PBS and incubated for 2 h with blocking and permeabilization buffer (1% BSA, 5% goat serum, 0.15% saponin (Sigma Aldrich)) ...
-
bioRxiv - Microbiology 2020Quote: ... in serum-free media supplemented with 0,1 μg/ml L-1-p-Tosylamino-2-phenylethyl chloromethylketone (TPCK)-treated trypsin (Sigma–Aldrich). The supernatant was harvested at 72 h post infection when cytopathic effects were observed (with around 50% cell death) ...
-
bioRxiv - Biochemistry 2020Quote: ... Bound scFv were detected using murine mAb 9E10 which recognizes the C-terminal c-myc tag (1:50 diluted in 2% MPBST) and a goat anti-mouse serum conjugated with horseradish peroxidase (HRP) (A0168, Sigma) (1:42000 dilution in 2% MPBST) ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells were blocked for 30 minutes in TBS with 0.1% Tween-20 (TBST) supplemented with 2% Normal Goat Serum (Sigma Aldrich). Histone H2AvD phosphoS137 antibody (Rockland cat # ...
-
bioRxiv - Immunology 2019Quote: The large intestine was opened longitudinally with blunt ended scissors and cut into 0.5cm segments before washing in 2% foetal calf serum (FCS) in Hank’s Balanced Salt Solution (HBSS) (Sigma, Life Sciences). To remove epithelial cells ...
-
bioRxiv - Immunology 2019Quote: ... and incubated at 37°C for 2 min before addition of 5 ml of serum-free L-15 containing 1 μg/ml DNase1 (Sigma) for 5 min at 37°C ...
-
bioRxiv - Neuroscience 2019Quote: ... Neurons were then treated with 10 mM glycine (Fisher BP381-1) in PBS, and incubated in a blocking solution (0.1% Triton X-100, 2% fetal calf serum (Sigma F4135), and 3% bovine serum albumin (BSA ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... The rehydrated slides were blocked with 2% horse-serum followed by incubation with primary antibodies (Mouse anti-S-glycoprotein antibody, 1:200 dilution, Millipore, Cat # MAB5676 ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were then diluted 1:2 in serum matrix for analysis with Milliplex Non-Human Primate Magnetic Bead Panel as per manufacturer’s instructions (Millipore Corporation). Concentrations for each cytokine were determined for all samples using the Bio-Plex 200 system (BioRad Laboratories Inc.).
-
bioRxiv - Microbiology 2021Quote: ... Viruses were amplified on MDCK cells cultured in serum-free DMEM containing 0.5 μg/mL L-1-p-Tosylamino-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin (Sigma–Aldrich). Stocks were titrated by plaque assays on MDCK cells.
-
bioRxiv - Immunology 2020Quote: ... Plates were then blocked with ELISA blocking buffer containing 2% w/v bovine serum albumin in PBS with 0.1% v/v Tween20 (PBS-T) (Sigma-Aldrich) for 1 h at room temperature ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The GC cell pellet was further suspended in 1-2 ml of serum free Dulbecco’s Modified Eagle Medium (DMEM) (Sigma, U.S.A) supplemented with 3 mM/ml of L-glutamine (Sigma ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... washed in dilutions of SSC (2x and 0.2x) and TNT prior to blocking (1-2 hours in blocking solution of 5% heat-inactivated horse serum (Millipore Sigma), 0.5% Western Blocking Regent (Millipore Sigma) ...
-
bioRxiv - Cell Biology 2021Quote: ... frogs were primed 2-14 days before ovulation by subcutaneous injection of 50 U pregnant mare serum gonadotropin (PMSG; Sigma) at the dorsal lymph sac ...
-
bioRxiv - Immunology 2020Quote: ... samples were incubated with 0,6% hydrogen peroxide to block endogenous peroxidases and were then incubated with 2% bovine serum albumin (Sigma, USA) to inhibit non-specific binding ...
-
bioRxiv - Microbiology 2021Quote: ... USA and inoculated in Vero 76 cells cultured in Minimum Essential Medium (MEM) (HyClone, Cytiva) supplemented with 2 % fetal bovine serum (FBS) (HyClone, Cytiva) and 50 μg/mL gentamicin (Sigma). Human influenza A virus strain ...
-
bioRxiv - Microbiology 2022Quote: ... was diluted to 1 μg/mL in 1% goat serum in PBS with 100 ng/mL of 4’6-diamidino-2-phenylindole (DAPI; Millipore Sigma) and added to cells for 60 min at RT ...
-
bioRxiv - Bioengineering 2022Quote: ... Cells were centrifuged at 300g for 5 minutes and the pellet was resuspended in 200 μL of sorting buffer (2 mM EDTA and 0.5% bovine serum albumin (BSA; Sigma-Aldrich) in DPBS ...
-
bioRxiv - Biochemistry 2022Quote: ... and serially diluted in DMEM media and incubated in 24-well plates with a semi-confluent culture of Vero cells (for ZKV samples) for 1 h at 37°C and covered with DMEM 2% fetal bovine serum + 0.8% of methylcellulose (Sigma, M0512) overlay for 4 days at 37°C and 5% CO2 incubator ...
-
bioRxiv - Developmental Biology 2022Quote: ... containing 0.1 % Tween-20 and 2 mM EDTA before incubating in a blocking solution (3 % acetylated bovine serum albumin (Sigma, MO), 0.1 % Tween-20 ...
-
bioRxiv - Microbiology 2022Quote: ... Cultures were incubated for 1 – 1.5 h at 37°C followed by addition of warm DMEM with 5% fetal bovine serum and 2% carboxymethyl cellulose (CMC; Sigma) to achieve a 1% CMC overlay ...
-
bioRxiv - Neuroscience 2022Quote: ... Longitudinal muscle-myenteric preparations were washed thoroughly in DMEM/F12 media containing fetal bovine serum (FBS, 2%, Sigma-Aldrich, F7524), penicillin-streptomycin (100U/ml ...
-
bioRxiv - Neuroscience 2022Quote: ... The cells were blocked for 1 hour with blocking buffer (Cyto-TBS + 2% bovine serum albumin (BSA, Sigma Aldrich, 1002695029)) and incubated with a primary antibody overnight at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... membranes were subsequently blocked for 2 h at room temperature with 5% bovine serum albumin (BSA) (Sigma-Aldrich, MN, USA) in Tris buffered saline (TBS ...
-
bioRxiv - Bioengineering 2023Quote: ... in PBS for 30 min and exposed to blocking buffer (2 wt% FBS, 0.5 wt% bovine serum albumin (Sigma Aldrich) and 0.1 wt% Tween-20 (Sigma Aldrich ...
-
bioRxiv - Bioengineering 2022Quote: ... and the cell pellet was re-suspended in 2 ml of triturating solution (containing 10 mg/ml bovine serum albumin [A7906, Sigma] ...