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Citations for Millipore Sigma :
6901 - 6950 of 10000+ citations for IL 3 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... we pre-treated 48-well plastic plates with 200 μL of 20 μg/mL human fibronectin (Milipore Sigma) for 10 minutes at 37 °C under 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: E0.5 embryos were cultured in embryo culture medium (EmbryoMax® Human Tubal Fluid, Sigma-Aldrich, # MR-070-D) under mineral oil for embryo culture (Sigma-Aldrich ...
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bioRxiv - Genomics 2023Quote: ... HUDEP-2 were maintained in Iscove’s modified Dulbecco’s medium (IMDM) supplemented with 330 μg/mL human holo-transferrin (Sigma), 10 μg/mL recombinant human insulin (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... Cells were seeded at 8-8.5×106 cells in 75 cm² or 16.5×106 cells in 150 cm² culture flasks (TPP) coated with 0.25% fibronectin from human plasma (Sigma) and 1% extracellular matrix from Engelbreth-Holm-Swarm murine sarcoma (Sigma ...
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bioRxiv - Immunology 2023Quote: Human IAV strains were propagated in MDCK cells in the presence of 1 µg/ ml TPCK trypsin (Sigma) and harvested after 72 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured using Fibronectin/Collagen/BSA ECM mixture-coated petri dishes (10µg/mL Human Fibronectin, Sigma Cat. No. F2006; 100µg/mL BSA, Sigma Cat.No.126575 ...
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bioRxiv - Cell Biology 2024Quote: ... was purchased from ATCC (TIB-202) and LX-2 human stellate cells were purchased from Sigma Aldrich (SCC064). LSECs were cultured in endothelial cell basal medium-2 (EBM-2 ...
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bioRxiv - Biophysics 2024Quote: ... For microfluidic surface endothelialization the channels were incubated overnight at 4°C with 10% human fibronectin (Sigma-Aldrich). HUVECs (Lonza ...
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bioRxiv - Microbiology 2024Quote: ... Baby Hamster Kidney (BHK) and Human Embryonic Kidney (HEK) 293 were grown in Dulbecco’s modified eagle’s medium (DMEM; Sigma) containing 10% foetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... cells were plated in 48-well plates pre-treated with 50 mg/mL human fibronectin (FC010-10MG, Millipore) and incubated for 24 hr ...
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bioRxiv - Cancer Biology 2024Quote: ... To this purpose culture dishes/glass slides were first coated with fibronectin (Sigma-Aldrich, Darmstadt, Germany, human plasma) at 5 µg/cm2 concentration diluted in sterile PBS ...
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bioRxiv - Physiology 2024Quote: ... were incubated in hypoxia condition in the hypoxia chamber with TGFβ1 (recombinant human TGFβ1: 15ng/ml, Cat #T7039, Sigma)25 for 72 h ...
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bioRxiv - Physiology 2024Quote: ... were incubated in hypoxia condition in the hypoxia chamber with TGFβ1 (recombinant human TGFβ1: 15ng/ml, Cat # T7039, Sigma)10 for 72 h ...
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bioRxiv - Immunology 2024Quote: ... purified human Tsen were pre-treated with or without the proteasomal inhibitor MG132 (10 µM; M7449; Sigma-Aldrich) for 30 minutes at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... we used commercially available ELISA kits: hs-CRP Human C-Reactive Protein ELISA Kit (#RAB0096, Sigma-Aldrich, Germany) and Mercodia Insulin ELISA (#10-1113-01 ...
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bioRxiv - Neuroscience 2024Quote: ... and 1% penicillin/streptomycin supplemented with 10 ng/ml EGF (Epidermal growth factor human, Sigma-Aldrich, Cat#E9644) and FGF (Fibroblast growth factor basic human ...
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bioRxiv - Cell Biology 2024Quote: ... and human embryonic kidney (HEK) 293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (D6546; Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Plant Biology 2019Quote: ... Cells were transfected with pcDNA3.1 and pEFl vectors using GeneJuice transfection regent (Merck Millipore, #70967-3) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were washed 3 times with TBST and exposed to HRP substrate (Immobilon Crescendo, Millipore, #WBLUR0100) for chemiluminescence detection using ChemiDoc™ MP Imaging System (Bio-Rad).
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TRANSITION OF PODOSOMES INTO ZIPPER-LIKE STRUCTURES IN MACROPHAGE-DERIVED MULTINUCLEATED GIANT CELLSbioRxiv - Cell Biology 2020Quote: ... and the Arp2/3 inhibitors CK-548 and CK-636 were from Sigma (St. Louis, MO). IL-4 was from Genscript (Piscataway ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was transferred to stagetips containing 3×14G plugs of 3MTMEmporeTM SDB-RPS substrate (Sigma) as described previously (Kulak et al. ...
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bioRxiv - Cell Biology 2020Quote: ... washed 3 × 5 mins in phosphate-buffered saline (PBS) with 0.1% Tween-20 (both Sigma-Aldrich) and probed for 1 hour at room temperature in the dark with IRDye® 800CW conjugated goat anti-mouse secondary antibodies (LiCOR Biosciences) ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were washed in PBS and incubated in 3% bovine serum albumin (BSA) (Sigma-Aldrich) diluted in PBS for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... Resulting supernatants were incubated with anti-FLAG M2 magnetic beads (Sigma, 3 h at 4°C). Beads were washed thrice with TUBE lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... shLmnA (produced in our lab), shLMNC (produced in our lab, 5′-TCTCCCACCTCCATGCCAAAG-3’) or shLMNB1 (Sigma clone NM_010721.1-956s1c1 ...
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bioRxiv - Cell Biology 2020Quote: ... washed and developed with chromogenic 5-Bromo-4-chloro-3-indolyl phosphate (BCIP, Sigma-Aldrich, B6149).
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bioRxiv - Cell Biology 2020Quote: ... organoids were rinsed twice with warm PBS and incubated with warm PBS containing 3% formaldehyde (Sigma) for 20 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Bead solution on the column was incubated with 3 µL 50U Thrombin (EMD Millipore, Novagen®) in 250 µL wash buffer overnight at 4° C on a rotator ...
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bioRxiv - Developmental Biology 2021Quote: ... After 3 washes for 5min each with 1ml DPBS−/− (DPBS without Mg and CaCl2, Sigma, D8537), 1ml 4% paraformaldehyde (PFA ...
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bioRxiv - Microbiology 2021Quote: ... Freshly purified NyxB was concentrated to 21 mg/ml on 3 KDa Amicon Ultra concentrators (Millipore). SeMet-NyxB was produced in M9 minimum medium and purified as above ...
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitation of 3×Flag-tagged proteins was performed using α-FLAG M2 affinity gel (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2021Quote: ... for phosphorylated histone 3 antibody staining (Chen et al., 2020) (pH3, Sigma # 05-806, 1:100), or with Lavdovsky’s fixative (3.7% formaldehyde ...
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bioRxiv - Neuroscience 2020Quote: ... then permeabilized for 30 min in PBS containing 1.5% TritonX100 and 3% hydrogen peroxide (Sigma; #31642). Then ...
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bioRxiv - Developmental Biology 2020Quote: ... After pre-incubation with 3% skim milk in PBS-T (PBS with 0.1% Tween20 (Sigma-Aldrich)) at RT for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... the supernatant was added to 3 ml anti-Flag (M2)-agarose (Sigma, St. Louis, MO, USA), and incubated at 4°C overnight ...
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bioRxiv - Physiology 2020Quote: ... and T3 (3,3’,5-triiodo-L-thyronine, > 95% HPCL, CAS number 6893-02-3, Sigma-Aldrich) were first dissolved in 0.1M NaOH and then diluted in 0.9% NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... and 3 μl were spotted onto plates with or without 100 μg/ml terbinafine (Sigma-Aldrich). Plates were imaged after 3–7 days of incubation at 30°C.
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bioRxiv - Molecular Biology 2020Quote: ... Following 3 x TBS-T washes the membranes were developed with Immunoblot Forte Western HRP (Millipore), according to the manufacturer’s instructions and imaged using an Amersham™ Imager 600 (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary antibodies (Anti-3-hydroxybutyryllysine [PTM Biolabs 1201], anti-p84 [GeneTex GTX70220], anti-α-Tubulin [Sigma T5168] ...
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bioRxiv - Cell Biology 2022Quote: ... MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was used for cell viability (Sigma, Israel).
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bioRxiv - Developmental Biology 2022Quote: ... Slices were washed in PBS for 3 times and incubated in 0.3% TritonX-100 (Sigma-Aldrich,) at room temperature (RT ...
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bioRxiv - Immunology 2021Quote: ... 3 μg pRSV-Rev and 6 μg of pRRL lentiCRISPR vectors using Poly(ethyleneimine) (PEI; Sigma). Media was replaced 24 hours post-transfection and harvested 24 hours later for filtration with a 0.45 μm filter (SteriFlip ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing α -syn were pooled and concentrated using a 3-kDa Amicon Ultra column (Millipore). Concentrated α -syn was loaded onto a gel filtration column (Superdex 200 ...
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bioRxiv - Immunology 2019Quote: ... cells were lysed in phosphoprotein lysis buffer containing 0.25% CHAPS with phosphatase inhibitor cocktail 3 (Sigma), protease inhibitor tablet (Qiagen ...
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bioRxiv - Pathology 2019Quote: ... at 4°C for 15 min and then subjected to blocking with 3% albumin (Sigma-Aldrich) at room temperature for 1 h ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were washed 3 times and incubated for 30 minutes with streptavidin Cy3 (Sigma, 1:500). All samples were sequentially washed and incubated with a mouse monoclonal anti-human CD31 (Dako ...
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bioRxiv - Developmental Biology 2020Quote: ... HDLECs were incubated in a 15-mL conical tube in presence of cytodex 3 beads (Sigma) at a ratio of 400 cells per bead in EGM-2MV medium (Lonza ...
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bioRxiv - Cell Biology 2019Quote: ... All cultures were tested every 3 months for mycoplasma contamination using the LookOut kit (Sigma, MP0035).
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bioRxiv - Cell Biology 2019Quote: ... Cultures were then treated with 50 ng/mL LMB dissolved in 7:3 MeOH:H2O solution (Sigma) for 45 minutes alongside a control of the equivalent volume of MeOH before imaging.
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bioRxiv - Microbiology 2020Quote: ... Pellets were resuspended in permeabilization buffer (3% (w/v) bovine serum albumin (BSA, Sigma-Aldrich, Germany) with 0.5% (v/v ...