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Citations for Millipore Sigma :
7151 - 7200 of 10000+ citations for IL 3 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and resuspending in 3 mL liquid YPD broth (50 g/L YPD broth powder [Sigma-Aldrich]). The culture was then incubated at 25°C overnight under constant rotation (20rpm).
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bioRxiv - Biochemistry 2023Quote: ... Primary antibodies were diluted in 1x TBS-T supplemented with 3% of BSA (Sigma Aldrich, #A7906). HRP-conjugated secondary antibodies were diluted 1:10.000 (anti-Mouse ...
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bioRxiv - Biochemistry 2024Quote: ... and the bound proteins were eluted with 150 μg/mL 3×Flag (Sigma-Aldrich, Cat# F4799) at 4 °C for 1.5 h ...
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bioRxiv - Bioengineering 2024Quote: ... The PCR product was then mixed with 1/3 volume 10 M ammonium acetate (Sigma, A1542) and 2 volume ethanol (Sigma ...
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bioRxiv - Bioengineering 2024Quote: ... Phalloidin and MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) were purchased from Sigma Aldrich. DMEM (Dulbecco’s Modified Eagle’s Medium) ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 0.5 mg/mL 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT, Sigma, USA) for 4 hrs at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... 10% glycerol and supplemented with Halt Protease Inhibitor Cocktail (Thermo #78430) and Benzonase (Millipore #70746-3). Cells were incubated at 4C shaking for at least 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... 10% glycerol and supplemented with Halt Protease Inhibitor Cocktail (Thermo #78430) and Benzonase (Millipore #70746-3). Cells were incubated at 4C shaking for at least 1 hour ...
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bioRxiv - Developmental Biology 2023Quote: ... Blocking and antibody incubation was done using 3% bovine serum albumin (BSA) (Sigma-Aldrich A7906-10G) and 0.3% TritonX (Sigma-Aldrich T8787 ...
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bioRxiv - Cancer Biology 2023Quote: ... 3-(4,5 dimethylthiazol-2-tl)-2,3-diphenyltetrazolium bromide (MTT) was purchased from Sigma-Aldrich (STL, USA). Bovine serum albumin (BSA ...
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bioRxiv - Bioengineering 2024Quote: ... and the supernatant was removed before cells were resuspended in 3 mLs of EBSS (Sigma; E3024). Cells were triturated 24x with a pulled Pasteur pipette in EBSS until homogenous ...
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bioRxiv - Neuroscience 2023Quote: ... non-specific sites were blocked for 30 min in 3% (v/v) goat serum (Sigma-Aldrich), in 0.1% (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... and Mannitol (3 g of Bacto Peptone [Becton Dickinson], 5 g of Yeast Extract [Sigma-Aldrich] ...
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bioRxiv - Cell Biology 2023Quote: ... Colonies were fixed in methanol-acetic acid 3:1 and stained with 5mg/ml GIEMSA (Sigma). Cell survival was expressed relative to control cells ...
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bioRxiv - Cell Biology 2023Quote: ... the tip of the micropipette was incubated overnight with 3% fetal bovine serum (Sigma-Aldrich, A7030) and incubated overnight.
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bioRxiv - Molecular Biology 2023Quote: ... coverslips were incubated for 3 hours in 100 μl of 2% acrylamide (AA; A4058, Sigma-Aldrich) + 1.4% formaldehyde (FA ...
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bioRxiv - Biochemistry 2022Quote: ... and controlled for purity by SDS-PAGE using NuPAGE 3-8% Tris-acetate gels (Sigma-Aldrich). AviTagged trimeric S and RBD proteins were biotinylated using the Enzymatic Protein Biotinylation Kit (Sigma-Aldrich).
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bioRxiv - Immunology 2023Quote: ... 100 μl/well of 2,2’-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt (ABTS, Sigma, A1888) was added to the plates and the reaction was stopped by the addition of 5% SDS ...
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bioRxiv - Microbiology 2023Quote: ... and resuspending in 3 mL liquid YPD broth (50 g/L YPD broth powder [Sigma-Aldrich]). The culture was then incubated at 25°C overnight under constant rotation (20rpm).
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bioRxiv - Molecular Biology 2023Quote: ... hybridised overnight at 37°C with 32P(CCCTAA)3 labelled telomere probe in PerfectHyb buffer (Sigma) before washing 4x at 37°C in 0.5X SSC ...
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bioRxiv - Immunology 2023Quote: ... Cells were treated for 24 h with DMSO as vehicle or inhibitors: hemicholinium-3 (Sigma-Aldrich), RSM-932a (Cayman Chemicals) ...
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bioRxiv - Microbiology 2023Quote: ... larvae were anesthetized in E3-MB containing 0.2 mg/ml Tricaine (ethyl 3-aminobenzoate; Sigma-Aldrich). For prolonged time lapse imaging ...
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bioRxiv - Cell Biology 2023Quote: ... retinas were washed 3 times 30 minutes with PBS 0.1% triton X-100 (X100, Sigma Aldrich). Secondary antibodies (see Table 1 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated at 37 °C for 3 min with 10 µM Paclitaxel/Taxol (Sigma-Aldrich) in BRB80 buffer (80 mM PIPES ...
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bioRxiv - Microbiology 2023Quote: ... and tissues digested in 3 mL complete HBSS-2 containing 0.5 mg/mL Collagenase-D (Sigma) at 37°C for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... plates were washed in PBS-Tw and non-specific binding blocked with 3% BSA (Sigma-Aldrich) in PBS for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Excess of chelator was removed by ultrafiltration (Amicon Ultra 0.5 ml, 3 kDa MWCO, Merck Millipore) using the same buffer conditions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... samples were enriched with 25nM 1-cyclohexyl-3-uriedo-decanoic acid (Sigma-Adlrich, St. Lousi MO) as an internal standard ...
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bioRxiv - Molecular Biology 2023Quote: ... and then treated for 3 hours with 50 µM MG-132 proteasome inhibitor (Sigma: cat# M7449).
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... After eight rinses in PBT (PBS with 3% Triton X-100, Sigma Aldrich, cat. no. X100), brains were blocked with 5% normal goat serum (NGS ...
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bioRxiv - Cell Biology 2023Quote: ... epithelium was extracted by incubation in DMEM supplemented with 3 mM DTT (Sigma Life Science, D9779), 1 mM EDTA (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... All reaction mixtures contained 250 nM of sirtuin (SIRT1-3, 5 Reaction Biology, SIRT4,6,7 Sigma-Aldrich), 10 µM furimazine (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: ... growth media was supplemented with 10μM of Nutlin-3 for at least 1 month (Sigma-Aldrich).
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bioRxiv - Microbiology 2023Quote: ... and treated with 3 × 45 s bead beating with glass beads (particle size ≤106 μm, Sigma) at maximum power and cooling on ice between cycles (MP Biomedicals™ FastPrep- 24TM ...
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bioRxiv - Microbiology 2023Quote: ... and 2-heptyl-3-hydroxy-4-quinolone (PQS) were obtained from Sigma Aldrich (St. Louis, MO). Triton X-100 and Tween-20 were used at 0.2% and 2% ...
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bioRxiv - Microbiology 2023Quote: ... Each aliquot was tested with one of the 3 substrates contained in the Chitinase kit (Sigma): 4-Methylumbelliferyl N,N′-diacetyl-β-D-chitobioside (substrate suitable for exochitinase activity detection or chitobiosidase activity) ...
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bioRxiv - Molecular Biology 2023Quote: ... and JUN degradation was induced by adding 100 µM Indole-3-acetic acid (IAA, Sigma-Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... a droplet of Pt-black electroplating solution containing 3 wt% chloroplatinic acid hexahydrate (206083, Sigma-Aldrich) and 0.3 wt% lead(II ...
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bioRxiv - Genetics 2023Quote: ... Fish were anaesthetised for live imaging using Tricaine methanesulfonate (3-amino benzoic acidethylester; Sigma Aldrich, E10521) at a final concentration of 0.016% in E3 embryo medium (5 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... 3 mL of LBv2 media without substance or test conditions (EMS [1:1,000](Sigma-Aldrich, #M0880) or MMS [1:10,000](Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Expression of the proteins of interest was induced by 3 μg/ml doxycycline (dox, Sigma-Aldrich) added to the culture medium ...
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bioRxiv - Bioengineering 2023Quote: ... They were then soaked in prepared 0.4% (v/v) 3-(Trimethoxysilyl)propyl methacrylate) (M6514, Sigma-Aldrich) in distilled H2O (dH2O ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: Cells were washed 3 times with PBS and then fixed with 4% paraformaldehyde (PFA, Sigma-Aldrich) for 10 min ...
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bioRxiv - Immunology 2023Quote: 3×104 DCs were plated in Costar low attached 96-well plate (Sigma-Aldrich, #CLS3474-24EA) and stimulated with LPS or LPS + L-LA or D-LA in presence of 20 μg/mL MOG35-55 for 6h ...
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bioRxiv - Cancer Biology 2023Quote: Cellular proliferation was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT; Sigma) assay and validated by viable cell counts.80
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bioRxiv - Cell Biology 2022Quote: ... the skin was minced with a scalpel and digested in 3 ml of trypsin (Sigma T4049) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2.5 mM of the maturation-blocking phosphodiesterase 3 inhibitor milrinone (2.5mM; Sigma Milipore M4659). GV (Germinal-Vesicle ...
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bioRxiv - Plant Biology 2023Quote: ... the samples and standard were silylated using Tri-sil reagent (3-3039, SUPELCO, Sigma-Aldrich, Germany) at 80°C for 20 min ...
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bioRxiv - Cancer Biology 2022Quote: ... cell viability was assessed using MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (Sigma-Aldrich). 10μl MTT (5 mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... the coverslips were rinsed by PBS and cells were fixed by 3% paraformaldehyde (#158127, Sigma-Aldrich). The cells were then permeabilized using 0.2% Triton X-100 (#04807423 ...