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Citations for Millipore Sigma :
6851 - 6900 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... mice were deeply anesthetized with avertin (2,2,2-tribromoethaol 1.25%, 2-methyl-2-butanol 0.78%; 20 µL/g, i.p.; Sigma Aldrich) and transcardially perfused with PBS followed by 4% paraformaldehyde in PBS ...
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bioRxiv - Microbiology 2024Quote: ... 2-hydroxyibuprofen (2-OH-IBU) and carboxyibuprofen (CBX-IBU) were obtained from Sigma-Aldrich (Steinheim, Germany). All chemicals and solvents used were of the highest purity available.
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bioRxiv - Neuroscience 2022Quote: ... The retinas were dissected and embedded in 2% low melting agar (2-hydroxymethyl agarose, Sigma Aldrich), mounted on a vibratome (DSK Microslicer ...
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bioRxiv - Cancer Biology 2024Quote: ... pH 2 buffer with 25% w/v (2-hydroxypropyl)-β-cyclodextrin (HP-β-CD, Sigma-Aldrich). Treatments were aliquoted into single and combined daily doses and frozen at -80 ºC ...
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bioRxiv - Developmental Biology 2024Quote: ... which was prepared as a 100% (w/v) stock solution in 2-methyl-2-butanol (Sigma), diluted 1:40 in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were incubated for 2 hours at room temperature with 2% normal donkey serum (Sigma, G6767) in PBS overnight at 4 °C with c-Fos primary antibodies (226003 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was applied on an already equilibrated 2-(2-pyridyl)ethyl columns (Sigma 54127-U) with 1 ml of 50% MeOH with 2% acetic acid ...
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bioRxiv - Plant Biology 2023Quote: ... 2% ß-mercaptoethanol and 2 tablets/10ml of complete EDTA free protease inhibitor (Sigma Aldrich, USA), 100μM E-64 cysteine protease inhibitor (Bera et al. ...
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bioRxiv - Biochemistry 2023Quote: 2-Pyridinecarboxaldehyde (1) and 6-(1-piperazinylmethyl)-2-pyridinecarboxaldehyde bistosylate salt were purchased from Sigma-Aldrich. 5-Ethynylpicolinaldehyde (“alkyne-2PCA” ...
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bioRxiv - Neuroscience 2024Quote: ... mice were deeply anesthetized with avertin (2,2,2-tribromoethaol 1.25%, 2-methyl-2-butanol 0.78%; 20 µL/g, i.p.; Sigma Aldrich) and transcardially perfused with PBS followed by 4% paraformaldehyde in PBS ...
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bioRxiv - Neuroscience 2024Quote: Under Avertin anesthesia (2,2,2-tribromoethaol 1.25%, 2-methyl-2-butanol 0.78%; 20 µL/g, i.p.; Sigma Aldrich) male A2A-Cre and D1-Cre mice (≥ 8 weeks old) ...
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bioRxiv - Microbiology 2019Quote: ... a “spot test” was applied by plating 100 μL of a Escherichia coli ATCC® 25922 ™ overnight culture over a lawn of Tryptic Soy Agar (TSA, Merck Millipore™), and then adding 100 μL of the virus filtrate in each of three thirds of the plate.
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bioRxiv - Systems Biology 2020Quote: ... on day 0 with a 50 µl volume of Escherichia coli O111:B4 LPS purified by ion exchange and with <1% protein and <1% RNA (Sigma Aldrich; catalog L3024), which was diluted in sterile ...
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bioRxiv - Cell Biology 2020Quote: ... 3 ml of new fully supplemented media was added either with 1 µg/ml LPS (Lipopolysaccharides from Escherichia coli 055:B5, γ-irradiated, BioXtra, Sigma-Aldrich, Austria) or 1 µl PBS per 1 ml medium as control ...
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bioRxiv - Biophysics 2022Quote: ... coli cells were grown in M9 medium (Green et al., 2012; Meyer and Schlegel, 1983) containing (15NH4)2SO4 (Sigma/Merck KGaA, Darmstadt, Germany) or (15NH4)2SO4 and 13C-D-glucose (Euriso-Top GmbH ...
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bioRxiv - Cell Biology 2019Quote: ... GST-Mm-RAB8A-Q67L and GST-Rn-RAB27A-Q78L proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore, Novagen, Darmstadt, Germany). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Immunology 2020Quote: Data was generated from immunizations of three juvenile New Zealand white rabbits: two rabbits with recombinant Escherichia coli Beta-gal (β-galactosidase from Sigma Aldrich, Inc.), and one rabbit with two linear peptides RNSEEARTDRPSQQLRSLNGE (peptide A ...
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bioRxiv - Synthetic Biology 2021Quote: ... Then 4 µL of ligation mix was transformed into 50 µL of home-made chemically competent E.coli DH5alpha obtained from New England Biolabs (C2987I) and selected on LB Agar plates containing 100 µg/mL Ampicillin (Sigma-Aldrich, A0166-25G)
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bioRxiv - Genetics 2021Quote: The coding regions of fur and mfur were amplified from mc2155 and mc251 genomic DNA and cloned into the Escherichia coli expression vector pET23b (+) (Novagen, Madison, WI, USA) inframe fused with a C-terminal His6-tag sequence to construct the plasmids pET23b-fur and pET23b-mfur ...
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bioRxiv - Microbiology 2021Quote: ... coli DH5α (Turbo) competent cells from New England Biolabs (Ipswich, MA) and NovaBlue GigaSingles™ Competent cells from Novagen (San Diego, CA, USA) were used for cloning ...
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bioRxiv - Cell Biology 2022Quote: ... coli strains XL 1 blue and K-12 MG1655 were cultured overnight in Luria Bertani (LB) broth media (Sigma Aldrich, cat. no: L3022). Overnight cultures were diluted to OD 600 ∼0.5 in RPMI1640 media supplemented with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2022Quote: ... coli DH10B (remaining plasmids) were used as cloning strains and electroporated with desalted ligation/Hot Fusion reaction (MF-Millipore membrane, VSWP, 0.025 μm).
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bioRxiv - Immunology 2023Quote: PBMCs were plated at a concentration of 1 million cells/ml and treated with Lipopolysaccharides from Escherichia coli O111:B4 (Sigma-Aldrich, cat# L4391) diluted in RPMI media (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... The following reagents were used for cell treatments at given concentrations: Lipopolysaccharide (Escherichia coli O55:B5, #L2880, Sigma-Aldrich, 100□ng□ml-1), recombinant human TNFα (rHuTNF ...
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bioRxiv - Neuroscience 2023Quote: P10 Nf1+/- and WT littermate male pups received a single intraperitoneal injection of 500 ug/kg lipopolysaccharide (Escherichia coli, serotype O26:B6, Sigma-Aldrich, Oakville, Canada) or a matched volume of 0.9% saline solution (sterile vehicle) ...
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bioRxiv - Physiology 2023Quote: ... Endotoxemia was induced by intraperitoneal (i.p.) injection of purified LPS extracted from the membrane of Escherichia coli 0111:B4 (Sigma-Aldrich, Saint Louis, USA) at a dose of 30 mg/kg as described previously (Zhao et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... both in the presence and absence of oestradiol (Complete medium with 10% FCS +/- 17-ß Oestradiol 1x10-8 M (Sigma Aldrich – cat no E-060)) in parallel ...
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bioRxiv - Microbiology 2021Quote: ... DBT cells were cultured in Eagle’s minimal essential medium (E-MEM) containing 5% fetal bovine serum and 1% tryptose phosphate broth (Sigma-Aldrich Co., St. Louis, MO, USA) at 37°C in 5% CO2 in humidified air.
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bioRxiv - Molecular Biology 2024Quote: Peripancreatic fat samples were embedded at the Louise Pelletier Histology Core Facility and tissue sections were stained with H&E (Epredia™ Modified Harris Hematoxylin, Fisher Scientific, Cat # 22-050-206; Eosin Y disodium salt, Sigma-Aldrich, Cat # E4382) in the Bruin Lab ...
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bioRxiv - Molecular Biology 2024Quote: ... 2b–f 3b,c and 4b,c and Extended Data Fig. 1b,c, 2a,c,d, 3a and 4d,e; Sigma-Aldrich, Extended Data Fig. 2b), and also supplemented with penicillin/streptomycin (P/S ...
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bioRxiv - Developmental Biology 2023Quote: ... final concentration 20 μM in Figure3 d-e and 40μM in Figure3 g-h) or Collagenase type IV (Sigma C5138, final concentration 20 μg/ml). Control explants were cultured with BME supplemented with the drug vehicle ...
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bioRxiv - Neuroscience 2023Quote: Human neuroblastoma cells SH-SY5Y cells (ATCC® CRL-2266™) were cultured in T75 flasks for propagation and then plated onto 100 μg/ml polyD-lysine (Millipore, A-003-E) coated 96-well glass bottom plates for imaging ...
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bioRxiv - Synthetic Biology 2021Quote: ... 2 mmol/L MgSO4 (Millipore Sigma), 0.1 mmol/L CaCl2 (Millipore Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... 2 mM glutamine (G8540, Sigma-Aldrich), 100 units/ml penicillin G (P3032 ...
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bioRxiv - Biophysics 2021Quote: ... and Mg(NO3)2 (Sigma-Aldrich) in 50 mM HEPES (pH 7 ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 μg of Flag (Sigma #F1804) antibody were added to each CTRL/TRIM67 IP samples ...
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bioRxiv - Cancer Biology 2021Quote: ... 2-Deoxyglucose (Sigma-Aldrich; 50 mM) was used to return ECAR to baseline ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM L-glutamine (Sigma-Aldrich), 5 mM pyruvate (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... sodium pyruvate and 2-mercaptoethanol (Sigma)) ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM Alanyl-glutamine (Sigma G8541), 1 mM sodium pyruvate (Merck TMS-005-C) ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM glutamine (both from Sigma) and 10 mM glucose (Merck ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 mg/ml heparin (Sigma)] for 5 days ...
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bioRxiv - Cell Biology 2020Quote: ... 2 µg/ml puromycin (Sigma-Aldrich) was added to select transduced cells.
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bioRxiv - Cell Biology 2020Quote: ... and 2 mM L-glutamine (Sigma) at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 50 μM 2-ME (Sigma-Aldrich), and 1% Penicillin/Streptomycin (Thermo Fisher).
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bioRxiv - Cell Biology 2020Quote: ... or 2% D-glucose (D; Sigma) or in Synthetic Complete (SC ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 % BSA (Sigma-Aldrich, # A7906) using a 1 ml pipet tip with the tip cut off to allow aspiration of larger pieces ...
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bioRxiv - Cell Biology 2020Quote: ... Ndiff Neuro-2 medium supplement (Millipore), B-27 medium supplement (Gibco) ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... 2 mM phenylmethylsulfonyl fluoride (Sigma-Aldrich) and phosphatase inhibitors (PhosSTOP ...
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bioRxiv - Cell Biology 2020Quote: ... 6-diamidino-2-phenylindole (DAPI) (Sigma) was used to eliminate dead cells ...