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Citations for Millipore Sigma :
6851 - 6900 of 10000+ citations for Rabbit Anti Human IgG+IgM+IgA Alexa Fluor 660 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: ... The incubation was followed by three 5 min washes and incubation with PLA probes anti-goat PLUS (DUO92003) and anti-rabbit MINUS (DUO92005) for 1 h at 370C as recommended in the Duolink PLA kit (Sigma). After three washes and cells were incubated with the hybridization solution containing DNA ligase for 30 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... Free-floating sections were incubated at 4°C for 16 hours with rabbit anti-Myc (1:500, C3956; Sigma-Aldrich) primary antibody in PBS/0.5% normal donkey serum/0.1% Triton X-100 ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by probing with respective goat anti-mouse or rabbit secondary antibodies conjugated with alkaline phosphatase (dilution 1: 5,000; Sigma-Aldrich) or conjugated with HRP (dilution 1:2000 ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by donkey anti-rabbit secondary antibody conjugated to horseradish peroxidase (“HRP”, Sigma GENA934, 1:5,000 in 1% milk-PBST). Aldolase was detected with anti-aldolase-HRP (Abcam ab38905 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... membranes were incubated with horseradish peroxidase-conjugated secondary antibodies in the blocking buffer for 1 h at room temperature (1:3,000 rabbit anti-goat, Sigma-Aldrich #A5420 or 1:3,000 goat anti-mouse ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies for all stainings were as follows: rabbit anti-TGFA (HPA042297, Sigma-Aldrich, dilution 1:50 and 1:100), rabbit polyclonal anti-pEGFR clone Tyr845 (07-820 ...
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bioRxiv - Neuroscience 2024Quote: ... protein isolation and immunoprecipitation was as previously described (Mohammed et al., 2016) using 1×107 OL-differentiated NSCs incubated with rabbit anti-OLIG2 (AB9610, Millipore) and using rabbit pre-immune IgG as control ...
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bioRxiv - Plant Biology 2024Quote: ... Then the membrane was incubated with another primary serum and revealed using an anti-rabbit immunoglobulin G coupled to alkaline phosphatase (Sigma). Data were acquired from three or four independent experiments and representative immunoblots are shown ...
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bioRxiv - Neuroscience 2024Quote: ... followed the same staining and mounting protocol described above but were incubated ON with a dilution of 1/2,000 anti-HA rabbit antibody (Sigma; H6908), and a 1/1000 dilution of AlexaFluor-488 donkey anti-chicken (Jackson InmunoResearch ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then incubated overnight in primary antibody Anti-Tyrosine Hydroxylase Antibody from rabbit (1:500, cat# AB152, Sigma Aldrich) diluted in blocking solution at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Hsp60 (1:1000, QED-BIOSCIENCE 11101) or glyceraldehyde-6-phosphate dehydrogenase (rabbit polyclonal anti-G6PDH antibody, 1:10000, Sigma A9521). Bound antibodies were detected using the appropriate horseradish peroxidase-conjugated secondary antibodies (1:10000 ...
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bioRxiv - Microbiology 2024Quote: ... followed by donkey anti-rabbit secondary antibody conjugated to horseradish peroxidase (“HRP”, Sigma GENA934, 1:5,000 in 5% milk-PBST). Anti-ATS and anti-NapL were detected as previously described (Nacer et al ...
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bioRxiv - Immunology 2024Quote: ... membranes were repeatedly washed with TBST and then incubated for 2 h with the anti-Rabbit HRP-conjugated secondary antibody (1:30.000 dilution; Sigma-Aldrich). Immunoreactivity was detected with Clarity Max ECL Substrate (Biorad ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The agarose affinity gels were washed with PBS five times and subjected to SDS-PAGE assay and Western blotting using rabbit anti-GFP antibody (Sigma), rabbit anti-V5 antibody (Merck ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 48–72 h at 4°C with PBS-T containing a combination of the following primary antibodies: rabbit polyclonal anti-CCK (1:1000, Sigma Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... anti-5-HTT (guinea pig, 1:50, HTT-GP-Af1400, Nittobo Medical Co, Japan) and anti-NeuN (rabbit, 1:200, MABN140, Millipore, Burlington ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with 0.05M PB containing 0.3% triton X-100 and the primary antibody (rabbit anti-FG, AB153-I, 1:10000, Sigma-Aldrich) at 4°Covernight ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 2 h incubation with HRP-conjugated goat anti-rabbit antibody (1:10,000, Sigma-Aldrich, St. Louis, MO, USA). The signal was visualized by chemiluminescent detection using ECL.
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bioRxiv - Neuroscience 2022Quote: ... and rabbit anti-ionized calcium binding adaptor molecule 1 (Iba-1) (1:350; Wako) to evaluate the inflammatory infiltrate and rabbit anti-myelin basic protein (MBP) (1:100; Millipore) to examine demyelination ...
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bioRxiv - Microbiology 2023Quote: ... The pre-cleared lysates were then incubated overnight at 4°C with polyclonal rabbit anti-TipR antibodies (1:400 dilution) (Kirkpatrick and Viollier, 2014) or monoclonal rabbit anti-HA antibodies (1:250 dilution) (Clone 114-2C-7, Merck Millipore). ...
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bioRxiv - Microbiology 2023Quote: ... and remaining lysate was end-over-end incubated with 5 µg of primary Ab overnight at 4°C [Rabbit-anti-HA (Sigma), Rabbit-anti-Flag (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were blocked with 3% BSA in PBS with 0.3% Triton X-100 for 1h at room temperature and subsequently incubated with primary antibodies (rabbit anti-glutamate, 1:500, Sigma; rabbit anti-SOM ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... the slices were washed three times with a blocking buffer containing 1% bovine serum albumin and 0.25% Triton-X in phosphate buffer saline (PBS) and then incubated with primary antibodies (anti-tyrosine hydroxylase, rabbit polyclonal, 1:1000, Merck Millipore; anti-D1R ...
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bioRxiv - Microbiology 2023Quote: ... the membranes were washed six times with TBST buffer and blotted with horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibodies (Sigma). After washing with TBST buffer again ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were blocked with 3% BSA/PBS for 1 hour and incubated with rabbit anti-Olig2 (1:500, Millipore), mouse anti-CC1 (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then washed in PBS and incubated with rabbit anti-TH primary antibody (1:200, Millipore AB152, RRID: AB_390204) overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... and incubated for 4 hours with an anti-rabbit secondary antibody coupled with Rhodamine (#SAB3700846 Sigma-Aldrich, dilution 1:200). Cells were incubated in AlexaFluor 488 phalloidin (#R37110 ...
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bioRxiv - Microbiology 2023Quote: ... Ultrathin sections of 50 nm were blocked with 5% fetal bovine serum/5% normal goat serum for 30 min and subsequently incubated with rabbit anti-HA antibody (Sigma) for 60 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: The primary antibodies used were: a rabbit polyclonal anti-GFP in a 1:400 dilution (Millipore Sigma, AB3080, lot: 3717722), a mouse monoclonal anti-NeuN in a 1:500 dilution that is a marker for mature neurons (Millipore Sigma ...
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bioRxiv - Microbiology 2023Quote: ... at 1:1000 dilution at room temperature for 1 hour followed by washes x 3 then staining with goat anti-rabbit HRP-conjugated antibody (Catalog #204903, Sigma) at 1:5,000 dilution at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with one or more of the following primary antibodies in blocking buffer overnight at 4°C (rabbit anti-TH, 1:2000; AB152, Merck-Millipore; sheep anti-TH ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated in primary antibody (Rabbit anti-GFP 1:1000 in blocking buffer, Cat# AB3080, Millipore Sigma, St. Louis, MO) overnight at room temperature on an orbital shaker ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were incubated with 1:5 diluted Duolink® in Situ PLA Probe anti-rabbit Plus (cat#DUO92002, Sigma-Aldrich) and anti-mouse Minus (cat#DUO92004 ...
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bioRxiv - Microbiology 2024Quote: ... was used in combination with a secondary anti-rabbit antibody conjugated to horseradish peroxidase (Sigma-Aldrich A8275 1:10 000). For visualization ...
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bioRxiv - Molecular Biology 2021Quote: ... ACC 57), K562 (human, DSMZ Germany ACC 10), and Molm-13 (human, DSMZ Germany ACC 554) were cultured in RPMI1640 (Sigma-Aldrich R8758) supplemented with 10 % heat-inactivated FCS and 1 % penicillin streptomycin mixture ...
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bioRxiv - Biochemistry 2019Quote: ... the GAP-334 region of human p120GAP (residues 715-1047) and the SOScat domain of human SOS1 (residues 564-1049) are cloned into pET15b (Novagen / EMD Biosciences) with an N-terminal polyhistidine (His ...
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bioRxiv - Cancer Biology 2019Quote: ... ECM proteins (collagen I and natural mouse laminin were purchased from Life Technologies, human plasma fibronectin from Millipore, human collagen IV from Sigma-Aldrich, recombinant human tenascin C ...
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bioRxiv - Immunology 2023Quote: ... Immune mediators in cell culture supernatant following CD3/CD28 bead stimulation were measured using a human premixed CD8+ T Cell Human 17-plex panel (Millipore, Temecula, California). All Luminex assays were analyzed using a MAGPIX Instrument and xPONENT software (Luminex ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% human serum AB (Merck Sigma-Aldrich), 300 IU/ml IL-2 ...
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bioRxiv - Immunology 2021Quote: ... and then treated with human complement serum (33.3%, Sigma) at 37oC for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg/ml human insulin (Sigma-Aldrich, Gillingham, UK), 0.4 μg/ml hydrocortisone ...
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bioRxiv - Microbiology 2020Quote: ... the working volume of human serum (Sigma-Aldrich, Australia) was divided in half ...
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bioRxiv - Genetics 2021Quote: ... 10 μg ml-recombinant human insulin (Sigma, 19278-5ML), 2 IU ml-heparin (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... supplemented with 1.25 mg/ml human insulin (Sigma #I9278), 1 % Penicillin-Streptomycin (Sigma #P4333 ...
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bioRxiv - Neuroscience 2022Quote: ... human basic fibroblast growth factor (bFGF) (Sigma, cat. #F0291), HEPES 1M (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 ng/mL of human bFGF (Sigma-Aldrich). Cells were incubated at 37 °C in a humidified atmosphere with 5% of CO2 ...
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bioRxiv - Bioengineering 2022Quote: ... Normal Human Dermal Fibroblasts (NHDFs) (Sigma-Alrdrich, Buchs, Switzerland), a non-cancerous human skin fibroblast cell line ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10 % heat-inactivated pooled human serum (Sigma) and 100 ng/ml macrophage colony stimulating factor (R&D systems) ...
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bioRxiv - Cancer Biology 2019Quote: ... human Collagen IV from Sigma-Aldrich (St. Louis, MO), recombinant human Tenascin C ...
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bioRxiv - Cell Biology 2020Quote: ... or shRNA directed against human IP6K1 (TRC0000013508, Sigma-Aldrich) were co-transfected with VSV-G and psPAX2 (a gift from Didier Trono ...