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Citations for Millipore Sigma :
7101 - 7150 of 10000+ citations for Rabbit Anti Human IgG+IgM+IgA Alexa Fluor 660 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... before being rinsed twice for 15 min each in PBS/BSA and incubated for 15 min in a 1/100 dilution (in PBS/BSA) of biotinylated goat anti-rabbit secondary antibody (Sigma Aldrich). Slides were rinsed as before and incubated with a 1/100 dilution (in PBS/BSA ...
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bioRxiv - Cell Biology 2020Quote: ... Detection was carried out by incubating with a 1:5000 dilution of a goat anti-rabbit antibody conjugated to peroxidase (Sigma Aldrich). For tagged proteins detection ...
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bioRxiv - Cell Biology 2020Quote: ... Detection was carried out by incubating with a 1:4000 dilution of a rabbit anti-rat antibody conjugated to peroxidase (A5795, Sigma-Aldrich). The membranes were then developed with the ECL Plus™ Western blotting detection system (PerkinElmer Life Sciences ...
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bioRxiv - Zoology 2020Quote: ... Each lysate was confirmed to express the appropriate recombinant protein at the expected size using an anti-V5 antibody produced in rabbit (Sigma V8137). Isoform specificity was tested via immunoblotting all cell lysates (empty vector control ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... directed against the N-terminal half of mouse AHR and the polyclonal goat anti-rabbit secondary antibody cross-linked to alkaline phosphatase (Sigma-Aldrich). The blot was developed using the AP Conjugate Substrate Kit (Bio-Rad).
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-FLAG clone M2 (WB 1:4000, IF 1:400; F1804) and rabbit anti-LC3B (WB 1:1000, IF 1:200; L7543) were from Sigma-Aldrich; goat anti-FLAG (1:400 ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were then washed with TBST buffer (5 x 1 min) and probed with secondary goat anti-rabbit antibodies conjugated with peroxidase (1:1000, Sigma #A6154). Blots were washed as described above and detection was carried out using Amersham ECL Western Blotting Select Detection Reagent (GE Lifescences #RPN2235 ...
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bioRxiv - Plant Biology 2021Quote: ... The PVDF membrane was then incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (diluted 1:10,000; Sigma-Aldrich, Stockholm, Sweden) for 1 h at room temperature followed by three washes with TBST and detection using a Agrisera ECL kit (catalogue no ...
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bioRxiv - Microbiology 2019Quote: For ChIP the following antibodies were used in this study: Rabbit polyclonal anti H3K9/K14-ac (Merck Millipore; Cat#06-599); Rabbit monoclonal anti H3K4-me3 (clone MC315 ...
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bioRxiv - Immunology 2019Quote: ... Beads were harvested and boiled in 40 μL of Laemmli sample buffer prior to western blot analysis with a polyclonal rabbit anti-PTPN2 antibody (SAB4200249, Sigma-Aldrich).
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Immunology 2020Quote: ... Blotted proteins were detected with a monoclonal antibody that recognizes SARS-CoV-2 S1 (SinoBiological) and a secondary anti-rabbit horse radish peroxidase (HRP)-conjugated antibody (Sigma Aldrich). Blots were developed with Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Developmental Biology 2020Quote: ... blocked with 3% BSA/TBST for 30 min and incubated with rabbit anti-Sox9 polyclonal antibody (1:500, EMD-Millipore, AB5535), rat anti-endomucin (Emcn ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue was then washed in PBS containing 0.03% tween-20 for two minutes following overnight incubation at 4°C with anti-versican GAGβ (1:100 dilution, rabbit source, Millipore AB1033). Tissue was then washed three times in PBS containing 0.03% tween-20 for fifteen minutes followed by secondary antibody staining for one hour at room temperature (anti-rabbit Alexafluor 488 ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were washed five times with TBS and incubated with secondary antibody (goat-anti-rabbit/mouse HRP-conjugated,1:2000 in TBS + 5% dry milk, Sigma Aldrich) for 45 minutes at RT ...
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bioRxiv - Immunology 2020Quote: ... were washed twice with 1 mL of buffer and sensitised with a 1/1000 dilution of rabbit anti-sheep red blood cell stroma antibody (Sigma; S1389). After a 30 °C / 30 minutes incubation ...
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bioRxiv - Genomics 2021Quote: ... The membranes were incubated overnight at 4°C with a 1:5000 dilution of monoclonal rabbit anti-HA antibodies (Sigma-Aldrich) or 1:5000 dilution of monoclonal rabbit anti-FLAG antibodies (Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... followed by a goat anti-rabbit secondary antibody conjugated with Cy3 for 1hr at room temperature (1:500, Millipore, Cat. #AP132C).
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed in 1xTBS and then in BS before probing with anti-rabbit antibody conjugated to Horse Radish Peroxidase (HRP) (1:10,000 in BS, Sigma, goat, A9169) for 60 minutes at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Surviving clones were screened via immunoblotting targeting TANGO1L (2 ng per mL rabbit polyclonal anti-MIA3, Sigma-Aldrich Prestige, HPA056816-100UL) and a rabbit polyclonal targeting anti-TANGO1-CC1 (1211 – 1440aa/exon9-15 shared between both TANGO1S/L (a gift from Kota Saito ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used are as follows: Anti-poly-ADP-ribose binding reagent/ PAR reagent (recombinant protein fused to rabbit Fc tag; Millipore, MABE1031), Rabbit monoclonal anti-Poly/Mono-ADP Ribose (anti-PAR/MAR ...
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bioRxiv - Neuroscience 2020Quote: ... Tissues were then incubated overnight with the following cocktail of primary antibodies to presynaptic components diluted in blocking solution with rocking: rabbit anti-NF 200 (1:200; Sigma, N4142), mouse anti-synaptophysin (1:300 ...
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bioRxiv - Neuroscience 2022Quote: ... The sections from the animals of different treatment groups were washed using PBS and permeabilized with 0.3% Triton X-100 and followed by incubation overnight at 4°C with the following primary antibodies:: rabbit anti-NeuN (1:100, Sigma-Aldrich), mouse anti-GFAP (1:500 ...
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bioRxiv - Genetics 2022Quote: ... TAP-tagged Rrp45 protein was detected with peroxidase anti-peroxidase (PAP) soluble complex antibody produced in rabbit (1:5000, Sigma-Aldrich). The 3-phosphoglycerate kinase (Pgk1 ...
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bioRxiv - Microbiology 2022Quote: ... α-PilC 20 and α-RomY antibodies were used together with goat anti-rabbit immunoglobulin G conjugated with horseradish peroxidase (Sigma) as secondary antibody ...
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bioRxiv - Molecular Biology 2022Quote: ... TAP- and HTP-tagged proteins were detected after a unique incubation of the membranes with Peroxidase Anti-Peroxidase Complex produced in rabbit (1:2,000; Sigma, Cat# P1291). Chemiluminescence signals were revealed on an Amersham™ Imager 600 (GE Healthcare ...
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bioRxiv - Developmental Biology 2022Quote: ... Specific protein detection was performed using diluted primary antibodies as follows: anti-NDRG1 @ 1:5000 (Sigma-Aldrich, catalog# HPA006881, rabbit polyclonal); anti-GAPDH @1:5000 (GeneTex ...
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bioRxiv - Plant Biology 2022Quote: ... Slides were then washed five times with PBS-BSA at 25°C followed by incubation in a 1:200 dilution of the tetramethylrhodamine-isothiocyanate-labelled goat anti-rabbit antibody (TRITC, Sigma-Aldrich) and fluorescein isothiocyanate-labelled goat anti-mouse antibody (FITC ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue was permeabilized/blocked in a solution containing 1% Triton-X and 10% NGS in PBS for 30 min and then incubated with an anti-HNE-Michael adducts rabbit polyclonal antibody (1:150 dilution, EMD Millipore) in a 10% NGS ...
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bioRxiv - Neuroscience 2024Quote: ... The membranes were then blocked in 5% milk in Tris Buffered Saline with 1% Tween (TBS-T) overnight at 4° C before being probed overnight at 4° C with Rabbit anti Magi-2 (1:1000, Sigma M4221); Mouse anti-NR1 (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were stained for c-Fos using rabbit anti-c-Fos antiserum (ABE457; Millipore, 1:1000; 1% NDS; 0.3% TxTBS) with Alexa Fluor 488 conjugated donkey anti-rabbit antiserum (AF488 ...
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bioRxiv - Neuroscience 2024Quote: ... or LY (1:500, #A5750, made in rabbit)-- and anti-sera to TH (1:10,000, MAB318, EMD Millipore Corp, Temecula, CA) or GAD1 (1:10,000 ...
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bioRxiv - Molecular Biology 2024Quote: Antibodies used for immunoblotting in this study include rabbit polyclonal anti-GFP (ab290, Abcam, 1:2,500 or Sigma, G1544, 1:1000), mouse monoclonal anti-GFP (JL8 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated in blocking solution (5% normal donkey serum and 0.3% Triton X-100 in PBS) for 30 minutes and then incubated in rabbit anti-TH polyclonal antibody (Millipore, Cat: AB152) diluted 1:1000 in 0.3% Triton X-100 in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted 1:2000 and incubated overnight at 4°C followed by an secondary anti-rabbit HRP conjugated antibody (12-348, Merck Millipore, Germany) diluted 1:5000 in PBST and incubated for 1 h at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... The blots were washed thrice with TBST buffer and incubated with HRP-conjugated anti-rabbit antibodies (dilution 1:20000; Sigma-Aldrich) at 25°C for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... α-PilT (66) (dilution 1:2000) and α-PilB (66) were used together with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulin G (Sigma) as a secondary antibody (dilution 1:10000) ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were incubated for 1 hour using the following antibodies: 1:500 rabbit anti-H3S10ph (Sigma-Aldrich, Merck, 06-570), 1:500 mouse anticyclin B1 (BD Pharmingen ...
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bioRxiv - Neuroscience 2023Quote: ... Poly-ubiquitinated CHTs were detected by stripping the membranes with Restore Plus buffer (Pierce) and incubating them with 1:2000 diluted rabbit anti-CHT polyclonal antibody (ABN458; EMD Millipore). The resulting chemiluminescent signal was acquired with a Chemidoc Touch Imaging System (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: ... washed three times with PBS-0.05% tween and followed by incubation with secondary antibodies directed against goat anti-mouse or rabbit HRP conjugated (1:5000, Sigma-Aldrich). The visualization of immunoreactive bands was performed using the enhanced chemiluminescence plus western blotting detection system (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: ... anti-HA (rabbit monoclonal from Cell Signaling Technology Inc., cat. no. 3724S; and mouse monoclonal from Sigma-Aldrich, cat. no. H3663), anti-myc (mouse monoclonal clone 9E 10 from the University of Iowa Hybridoma Bank) ...
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bioRxiv - Genomics 2023Quote: ... Revelation of the primary antibodies was performed by incubation with goat anti-rabbit Horseradish peroxidase-conjugated secondary antibody (Ref#A0545, Sigma-Aldrick) at 1/10000 ratio in blocking solution for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: Cytoplasmic and nuclear fractions were then subjected to western blotting and labelled with rabbit monoclonal anti-GAPDH (Sigma, G9545, 1:5,000) antibody and rabbit polyclonal anti-LSD1 antibody (Abcam ...
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bioRxiv - Synthetic Biology 2023Quote: ... in TBS-T for 1 h at room temperature and stained overnight at 4 °C with a rabbit anti-FLAG antibody (1:1000 dilution, Millipore F7425). After removing unbound antibodies by three 5-minute washes with TBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... pTyr (clone 4G10, 05-321), and HA (clone HA-7, H3663) and rabbit polyclonal anti-SEPTIN12 (HPA041128) were from Sigma-Aldrich. Rabbit and mouse monoclonal anti-DYKDDDDK (clone D6W5B ...
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bioRxiv - Neuroscience 2023Quote: ... then washed with PBS again and incubated for 48h with a rabbit anti-TH antibody (1:1000, Millipore Cat# AB152, RRID:AB_390204) at 4°C ...
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bioRxiv - Genetics 2023Quote: ... as previously described.17 The following primary antibodies were used for immunohistochemistry: rabbit anti 2A peptide (1:7500, ABS31, Sigma-Aldrich), rabbit anti Ki67 (1:60 ...
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bioRxiv - Physiology 2023Quote: ... and incubated in anti-PEMT rabbit polyclonal antibody (1:1000, Bio-techne #NBP1-59580) or anti-α-TUBULIN mouse monoclonal antibody (1:10,000, Millipore-SIGMA #T5168) in 5% dairy milk ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were incubated in primary antibody solution (1:1000 rabbit anti-GFP or 1:1000 rabbit anti-RNA Pol II CTD repeat YSPTSPS (phospho S5) in blocking buffer (1:10 Western Blocking Reagent (Sigma 11921673001) in PTx) ...
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bioRxiv - Genomics 2023Quote: ... and cells were resuspended in 100 μL dig-wash buffer containing 2 mM EDTA and a 1:50 dilution of rabbit anti-mouse antibody (Sigma, M7023) and incubated at RT for 1 hour with slow rotation ...