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Citations for Millipore Sigma :
6851 - 6900 of 10000+ citations for 6 METHOXY PYRIDINE 3 SULFONYL CHLORIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Cultures were grown at 37°C 6% CO2 and sub-confluent cultures were trypsinised with 0.25% Trypsin-EDTA (Sigma). For NHBE-A differentiation in Air Liquid Interface (ALI ...
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bioRxiv - Microbiology 2023Quote: ... β-mercaptoethanol (99%) for 6 h at 4 ℃. The proteins were concentrated by Amicon Ultra (M.W. 3,000 Da) (Sigma-Aldrich) and stored at −80 ℃.
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bioRxiv - Biochemistry 2023Quote: ... the ventricles were minced and dissociated into single cells by repeated sequential pancreatin (Sigma, [#8049-47-6, lot#SLBN2032V]) and collagenase II (Worthington ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were denatured (95 °C for 10 minutes) with 6×sample buffer [0.375 M Tris pH 6.8 (Sigma-Aldrich), 12% SDS (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... 1.5 polymer coverslip) or 6 (μ-Slide VI 0.4 ibiTreat: 1.5 polymer coverslip) channels were coated with fibronectin (SIGMA) for 1 hour and seeded with 100 000 or 50 000 cells per channel respectively ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were grown on 6-well plates previously coated overnight with a 0.1 mg/ml solution of poly-L-ornithine (Millipore), an important requirement to prevent cell detachment ...
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bioRxiv - Immunology 2023Quote: ... islets were incubated (37°C for 6 min) in a non-enzymatic cell dissociation solution (Sigma-Aldrich; #C5914-100ML) then vortexed to disrupt the islets.
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bioRxiv - Genetics 2023Quote: ... at 37°C in a 6-well plate and then 4mL of media was added (High glucose DMEM (Sigma), 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... we removed the zona pellucida by washing the oocytes in 4-6 drops of acidic Tyrode’s solution (Millipore Sigma) and rinsed them in 4-6 drops of CZB media with the corresponding drug(s ...
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bioRxiv - Cancer Biology 2023Quote: Five hundred A673 or SKES1 cells transduced with shRNAs targeting KCNN1 or shRNA non-targeting control were seeded in 6-well plates after 72 hours or 7 days of doxycycline treatment (0,5 µg/mL, Sigma). Cells were then incubated for 8 to 10 days under standard conditions (humidified incubator at 37°C and 5% CO2) ...
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bioRxiv - Biochemistry 2023Quote: ... snap-frozen eluates were thawed with the addition of 100 μL of 6 M guanidinium/HCl in 100 mM Tris/HCl pH 8.5 containing protease inhibitor cocktail (Sigma). Samples were washed with 6 M guanidine/HCl 100 mM Tris/HCl ...
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bioRxiv - Bioengineering 2023Quote: ... sGAG content was determined through a standard curve using chondroitin-6-sulphate (sodium salt from shark cartilage, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2023Quote: ... Dye transfer from DiI-stained ALL cells cultured in transwells towards MSCs cultured in 6-well plates (Sigma Aldrich) was determined after 40 hours by flow cytometry ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then seeded on 6 mm diameter glass coverslips previously coated with 0.01% poly-L-lysine (Sigma, USA). 24h after seeding ...
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bioRxiv - Immunology 2023Quote: ... Final cell numbers were 6 x 107 cells/ml for large cultures (24-well size transwell plate, Millipore-Sigma) or 2 x 107 cells/ml for small cultures (96-well size transwell plate ...
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bioRxiv - Immunology 2023Quote: ... Final cell numbers were 6 x 107 cells/ml for large cultures (24-well size transwell plate, Millipore-Sigma) or 2 x 107 cells/ml for small cultures (96-well size transwell plate ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were washed with PBS and incubated with secondary antibodies for 2 h at RT in 2.5% BSA and 0.1% triton-X in PBS followed by counterstaining with 4’,6-diamidino-2-fenylindool (DAPI) (1µg/µl, Sigma, D9564) for 15 minutes at RT ...
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bioRxiv - Physiology 2023Quote: ... Unspecific antibody binding was blocked with 6% donkey serum or 4% goat serum (Sigma-Aldrich, St Louis, MO, USA), and 2.5% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... LSFM imaging was performed in ethyl cinnamate for index matching (Millipore Sigma, cat.no.: 112372, CAS number: 103-36-6) using the same imaging parameters.
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bioRxiv - Molecular Biology 2024Quote: ... the mice were subcutaneously injected with vitamin D3 (6 or 8 x 105 IU/kg; C1357, Sigma-Aldrich, USA) daily for three days (n=5-8 per group) ...
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bioRxiv - Microbiology 2023Quote: ... spleen cells from a single mouse were harvested and stimulated for 6 h with 16 nM PMA (Sigma-Aldrich) and 1 μM ionomycin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: 293T cells were seeded into 6-well plates and transfected with replicon plasmid using Xtreme-Gene9 transfection reagent (Sigma). 24 hours post-transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were split into 6-well plates at a density of 0.5×106 cells/well and subjected to 2µg/mL puromycin (Sigma P8833) for selection of infected cells ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were washed in PBS and incubated in 5 μg/ml 4’,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich) in PBS for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubating with the primary antibody (mouse monoclonal anti-acetylated tubulin, 6-11B-1, 1:1000, Sigma T6793) and then with the secondary antibody (goat anti-mouse IgG-Alexa Fluor 488 ...
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bioRxiv - Cell Biology 2023Quote: The CCl4 model of cirrhosis was prepared as previously described.23 Male C57Bl/6 mice were treated three times a week for 18 weeks with 0.1 ml of 40% CCl4 (Sigma) in olive oil by oral gavage.
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bioRxiv - Biochemistry 2023Quote: ... The 1x Running Buffer (pH5.7±0.1 at 25 °C) contains 400 mM 6-aminohexanoic acid (EACA) (Sigma-Aldrich Canada), 2 mM Triethylenetetramine (TETA ...
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bioRxiv - Genetics 2024Quote: ... Samples were washed with RIPA 3 × and eluted by boiling for 10 min in 300 mM Tris at pH 6.8 with 6% SDS and 600 mM dithiothreitol (DTT, Millipore) to release crosslinks ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were washed in PBS and incubated in 5 μg/ml 4’,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich) in PBS for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... A 30-min incubation of PBS supplemented with 1 µg/mL of 4′,6-diamidino2-phenylindole (DAPI) (Sigma-Aldrich) was performed ...
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bioRxiv - Developmental Biology 2024Quote: ... and seeded at 8X105-1X106 cells per well in a 6 well plate coated with Matrigel (Millipore Sigma, CLS354277) 24 hours prior to differentiation treatment ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 μg viral plasmid were diluted in 6 μL X-tremeGENE 9 DNA transfection reagent (Sigma-Aldrich, 6365787001) and 150 μL DMEM and incubated at room temperature for 15 min before being added to the cells ...
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bioRxiv - Cell Biology 2024Quote: ... we use primary anti-acetylated tubulin antibody from mouse (monoclonal 6-11 B-1, T7451 Sigma, 1/1000 dilution) as well as primary anti-tyrosinated tubulin antibody from mouse (monoclonal TUB-1A2 ...
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bioRxiv - Neuroscience 2024Quote: ... and the remaining pellet was washed once with 50 mM TBS, centrifuged (200,000x g, 22 min at 4 °C) and then solubilized in 6 M GuHCl (Sigma) with protease inhibitors ...
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bioRxiv - Neuroscience 2024Quote: ... and then immersed in OCT for the preparation of transversal cryosections (10µm) that were mounted on microscope slides treated with 6% silane (cat#440140, Sigma). Electroporated retinas ...
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bioRxiv - Pathology 2024Quote: ... of mosquito EV-lipid extract or of EV-lipid fraction 6 was treated with 1 U of SMase (Sigma) for 1h at 37°C ...
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... 250,000 cells per well were seeded in 6-well plates 24 hours before adding MG- 132 (C2211, Sigma-Aldrich) at a concentration of 0.375 µM dissolved in DMSO ...
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bioRxiv - Microbiology 2024Quote: The 10-ng internal CL standard (14:0-14:0-14:0-14:0) (Sigma-Aldrich, 63988-21-6) was added to lipid extracts for lipidomic analyses ...
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bioRxiv - Biochemistry 2024Quote: Proteins were extracted from 300,000 to 400,00 HUVEC cells (per one well of a 6-well plate) with RIPA buffer (Millipore Sigma) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... seedlings were grown in MS media supplemented with sucrose and various concentrations of 6-benzylamino purine (BA, Sigma, No. B3408) or sodium hydroxide as control.
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bioRxiv - Cancer Biology 2021Quote: ... before the addition of MNNG to inhibit the repair of O6meG adducts by O-6-methylguanine-DNA methyltransferase (MGMT, Sigma). After 48h MNNG treatment ...
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bioRxiv - Bioengineering 2021Quote: ... The secondary antibodies were washed away with PBS (3x 5 min) whereafter 4’,6-diamidino-2-phenylindole (DAPI, 1:500, Sigma) was added for 10 minutes to localize the cell nuclei ...
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bioRxiv - Biophysics 2020Quote: Purified K2P2.1cryst was concentrated to 6 mg ml-1 by centrifugation (Amicon Ultra-15, 50 kDa molecular mass cut-off; Millipore) and crystallized by hanging-drop vapor diffusion at 4 °C using a mixture of 0.2 μl of protein and 0.1 μl of precipitant over 100 μl of reservoir containing 20–25% PEG400 ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were washed 2× in PBS and then counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; 1:10 000; Sigma) for 10 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... the medium was changed to S1 medium consisting of basal medium supplemented with 6 μM CHIR99021 (Sigma, Cat No. SML1046). Basal medium was prepared by adding 1x GlutaMax supplement (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: The following monoclonal antibodies were used for immunofluorescence: anti-acetylated tubulin (mouse; clone 6-11B-1; Sigma-Aldrich; 1:500), anti-ubiquitin clone FK2 was used in all experiments except where indicated (mouse ...
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bioRxiv - Neuroscience 2021Quote: ... saline or 6-OHDA (5 μg/μL; 2 μL in total, at a rate of 0.5 μL/min, Sigma, Canada) were injected unilaterally in the dorsal striatum ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were incubated with 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, cat. D8517, Sigma Aldrich, St. Louis, MO, USA) diluted 1:3000 in PBS 1X and then mounted with Vectashield antifade medium (cat ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were fixed using 70% Ethanol and re-suspended in PBS containing 1 μg/ml 4’,6-diamino-2-phenylindole (DAPI, Sigma) and analyzed by microscopy ...
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bioRxiv - Microbiology 2020Quote: ... cells were analyzed using a Guava EasyCyte 6/2L bench cytometer equipped with 488 nm and 532 nm lasers (Millipore). Flow cytometry plots are representative of at least three independent experiments.