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Citations for Millipore Sigma :
6751 - 6800 of 10000+ citations for Recombinant Human CYCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... and proteins denatured using SDS/PAGE loading buffer (bio rad) and 2-mercaptoethanol (Sigma-Aldrich). Proteins were separated by gel electrophoresis (NW04120BOX ...
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bioRxiv - Molecular Biology 2020Quote: ... Eluted proteins were concentrated using 10-kDa molecular weight cut off filters (Amicon Ultra, Millipore) and washed with ddH2O ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were purified from the lysate with anti-FLAG M2 affinity gel (Millipore-Sigma, USA) and eluted with 0.2 mg/ml 3xFLAG peptide in elution buffer (50 mM HEPES/KOH pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were purified from the lysate with anti-FLAG M2 affinity gel (Millipore-Sigma, USA) and eluted with 0.2 mg/ml 3xFLAG peptide in elution buffer (50 mM HEPES/KOH pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: The DRM and DSM fractions119 were separated using the CelLytic MEM Protein Extraction Kit (Sigma). 600 μl of lysis and separation buffer were added to 50 μl of resuspended membrane and incubated at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal antibody against HCoV-OC43 nucleocapsid (NP) protein was purchased from Millipore (Cat.No. MAB9012). Rabbit polyclonal antibody against human LY6E was obtained from proteintech (Cat.No ...
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bioRxiv - Microbiology 2019Quote: ... PVDF membrane-bound proteins were first probed with mouse anti-HA (1:3000; Sigma, H3663) and rabbit anti-GAPDH (1:5000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred to a PVDF membrane (Immobilon-P or Immobilon-FL, Millipore, Burlington, MA) and blocked for 1 h at room temperature in 5% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... coli wild type (wt) Hfq protein and its mutants were expressed from pET15b vector (Novagen) and purified as described [38] ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were transferred onto methanol-preactivated Immobilon-P PVDF membranes (pore size 0.45 μm; Millipore) using a semi-dry transfer cell or a wet transfer tank (Bio-Rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... The sypro orange protein gel stain was purchased from Sigma Aldrich (Merck KGaA, Darmstadt, Germany). The integrated potato culture medium ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were transferred from the polyacrylamide gel to a PVDF membrane (Merck Millipore, Massachusetts, USA). Membranes were blocked with Rockland blocking buffer (Biomol GmbH ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with endoproteinase Lys-C (Wako Chemicals) and sequencing grade-modified trypsin (Sigma). Protease digestion was stopped by addition of trifluoroacetic acid to 0.5% and precipitates were removed by centrifugation ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... RIPA buffer was not used and the protein concentration was measured with Bradford assay (Millipore).
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bioRxiv - Biophysics 2020Quote: Hen egg white Lyz and bovine β-Lac proteins were both purchased from Sigma-Aldrich and used with no further purification ...
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bioRxiv - Biophysics 2020Quote: ... Protein was concentrated and buffer exchanged on Amicon Ultracel-10K centrifugal filters (Millipore; Darmstadt, Germany), dialyzed exhaustively into 10 mM MOPS ...
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bioRxiv - Cancer Biology 2021Quote: ... 80 μg protein extract from each sample was reduced with 50 mM dithiothreitol (Sigma, #43815) at 95 °C for 10 min and then alkylated with 100 mM iodoacetamide (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Equal amount of protein was loaded to the anti-Flag M2 Magnetic beads (M8823, Sigma) and anti-HA Magnetic beads (88836 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated by SDS-PAGE (8% acrylamide gel) and transferred to nitrocellulose membranes (Millipore). Membranes were incubated overnight at 4°C with a primary antibody diluted in Tris-buffered saline plus 0.1% Tween 20 (TBST) ...
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bioRxiv - Cell Biology 2021Quote: ... The dialysed protein was concentrated in a 10 kDa cut off Amicon Ultra-15 (Millipore), quantitated using BCA ...
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bioRxiv - Biophysics 2022Quote: ... The protein samples were concentrated to 30mg/ml using 100-kDa MWCO AmiconUltra concentrators (Millipore) for crystallization trials and further analysis.
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bioRxiv - Cancer Biology 2022Quote: ... and then the proteins were transferred onto polyvinylidene difluoride membranes (Merck Millipore, Billerica, MA, USA).
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bioRxiv - Biochemistry 2022Quote: WT and mutant Streptococcus pyogenes Cas9 proteins were expressed in E.coli Rosetta 2 (DE3) (Novagen) for 16 hours at 18 °C as fusion proteins with an N-terminal His6-MBP-TEV tag ...
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bioRxiv - Pathology 2022Quote: ... Proteins separated via SDS-PAGE gels were blotted onto PVDF membranes (Millipore, Cat. No. ISEQ00010). To reduce non-specific antibody binding and reduce background ...
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bioRxiv - Biochemistry 2022Quote: ... Purified protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 50 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... protein samples were subjected to 10% SDS-PAGE and moved to PVDF films (Millipore, America). After the blockade with 5% milk ...
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bioRxiv - Biochemistry 2022Quote: ... Purified protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 50 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Molecular Biology 2022Quote: ... the eluted proteins of IP were transferred to a 10 kD centrifugal spin filter (Millipore), and followed by washing with 8 M urea 200 μL for three times ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were separated by 15% SDS-PAGE gel and blotted onto a nitrocellulose membrane (Millipore). The membrane was first stained with Ponceau S to show protein loading before blocking and incubation with first antibodies ...
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bioRxiv - Pathology 2022Quote: ... XO Activity was expressed as units/mg protein using native XO from buttermilk (Merck Millipore) as standard.
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bioRxiv - Pathology 2022Quote: ... 15-30 μg protein per sample were denatured with 5 % vol/vol 2-mercaptoethanol (Sigma) for 5 min at 95 °C before they were loaded in Bolt™ 4–12 % Bis-Tris gels (Thermo Fisher) ...
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bioRxiv - Pathology 2022Quote: ... Equal amounts of proteins were incubated with anti-FLAG M2 affinity gel (Sigma Aldrich, A2220) and processed by Western blotting.
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bioRxiv - Genetics 2022Quote: ... 200 μg of protein extract was incubated with 30 μl anti-HA magnetic beads (Sigma) at 4°C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted protein solution was applied to a MonoQ 10/100 column (Sigma-Aldrich, Merck), equilibrated in MonoQ buffer A (40 mM Hepes ...
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bioRxiv - Developmental Biology 2022Quote: ... the protein extract was loaded onto the centrifugal filter CO10 kDa (Merck Millipore, Darmstadt, Germany), and detergent were removed by washing five times with 8M Urea (Merck ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were electrophoretically separated using 10% SDS gel and transferred onto nitrocellulose membrane (Millipore, USA). The membranes were blocked with 5% Non-Fat Dry milk in PBST (1X PBS with 0.1%Tween-20 ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein extracts from tumor tissues were prepared by homogenization in RIPA buffer (Sigma-Aldrich, USA) containing a protease inhibitor cocktail (Roche ...
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bioRxiv - Immunology 2022Quote: ... The protein bands on the PVDF membranes were observed using an enhanced chemiluminescence kit (Millipore) and quantified using Image J software (NIH ...
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bioRxiv - Biophysics 2022Quote: ... The purified protein was then reduced with tris(2-carboxyethyl)phosphine (TCEP, obtained from Sigma), a strong ...
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: ... The proteins were then transiently transfected (pBiex-1; Escort IV, Sigma-Aldrich, St. Louis, MO) into Sf9 cells ...
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bioRxiv - Cancer Biology 2022Quote: Total protein extracts were obtained from cell lines and tumour specimens using RIPA (Sigma-Aldrich) buffer and subjected to SDS-PAGE ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies (listed in Table S4) were pre-conjugated to magnetic protein A/G beads (Millipore). Chromatin immunoprecipitation was carried out overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were stained with Ponceau S (0.1% Ponceau reagent [Sigma, P3504] with 5% acetic acid). Incubation with primary antibodies was conducted overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... to be subsequently treated with Iodacetamide for protein alkylation (Sigma-Aldrich, St. Louis, MO, USA) and ultimately digested with 1.25 µg of recombinant Trypsin (sequencing grade ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were reduced with 60 µL 40 mM dithiothreitol (DTT; Sigma-Aldrich, Taufkirchen, Germany) in 50 mM TEAB ...
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bioRxiv - Microbiology 2021Quote: ... protein extracts were incubated for 45 min with 1%v/v Benzonase Nuclease (SIGMA E1014). For the FASP digestion ...
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bioRxiv - Cell Biology 2021Quote: ... crosslinked to Protein A/G PLUS-Agarose (SantaCruz) using 20 mM dimethyl pimelimidate (Sigma Aldrich) at 4°C for overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged proteins were eluted by 60 μl of 3x FLAG® Peptide (Sigma F4799) (diluted in Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2021Quote: ... protein was dialyzed into REMSA buffer [10mM HEPES pH 7.3 (Sigma-Aldrich, Cat. No. H3375), 20 mM KCl (Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Separated proteins were then electro-transferred onto a polyvinylidene fluoride (PVDF) membrane (0.2 µm; Millipore). After blocked with 5 % skim milk in TBST (50 mM Tris-HCl ...