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Citations for Millipore Sigma :
6751 - 6800 of 10000+ citations for Mouse FAM117A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used: mouse anti-PV (1:1000, Sigma, #P3088), rabbit anti-PV (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... anti-NeuN antibody (mouse, 1:400, #MAB377; Merck Millipore, Burlington, MA, USA), or anti-IL2Rα antibody (goat ...
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bioRxiv - Neuroscience 2024Quote: ... mouse IgG1 anti-parvalbumin (1:500; PVALB-19; (Sigma-Aldrich, MO, USA). After three washes with PBS for 10 min each ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used: mouse anti-Flag F7425 (Sigma-Aldrich, F7425); rabbit anti-Phospho-PAK4/5/6 (pSer474 ...
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bioRxiv - Neuroscience 2024Quote: ... Antibodies utilized for western blot: fly samples: mouse β-actin (Sigma Prestige); rabbit LC3 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Secondary antibodies are anti-Mouse IgG-HRP (Sigma-Aldrich, #A5278; 1/5000), anti-Rabbit IgG-HRP (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: The following antibodies were obtained commercially: mouse monoclonal anti-myc 9E10 (Sigma), mouse monoclonal anti-dsRNA J2 (Jena Bioscience) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... mouse anti-Nestin (MAB353, 1:800; Sigma-Aldrich, St. Louis, MO, USA), mouse anti-Tmem119 (ab209064 ...
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bioRxiv - Molecular Biology 2024Quote: ... Mouse E14 ESCs were infected with concentrated lentiviral particles with polybrene (Sigma) at the concentration of 8 μg/ml ...
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bioRxiv - Microbiology 2024Quote: ... The samples were incubated with mouse anti-HA (1:500, Sigma, USA), rabbit anti-TgGAP45 (1:300 ...
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bioRxiv - Immunology 2024Quote: ... Secondary antibody was goat anti-mouse IgG HRP labelled (1:2500; Millipore). The reaction was developed by ELISA as above ...
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bioRxiv - Molecular Biology 2024Quote: ... the membrane was also probed with mouse anti-GAPDH (EMD Millipore; AB2302).
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bioRxiv - Molecular Biology 2024Quote: ... Adult mouse temporal bones were decalcified in Osteosoft® (Millipore Sigma 1017281000) for 24-48 h at 37° C ...
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bioRxiv - Microbiology 2024Quote: ... at 4°C before either antibody (mouse anti-FLAG, Sigma-Aldrich F1804) or glutathione sepharose was added for overnight incubation ...
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bioRxiv - Biochemistry 2024Quote: ... anti-β-actin mouse monoclonal (AC-15) (Sigma-Aldrich, A1978, 1:1,000), anti-PXDN rabbit polyclonal (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... the product was digested with NcoI and NotI and ligated into the pET45b(+) expression plasmid (Novagen/EMD Millipore). Integration in the pET45b(+ ...
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bioRxiv - Bioengineering 2022Quote: ... the product was digested with NcoI and NotI and ligated into the pET45b(+) expression plasmid (Novagen/EMD Millipore). Integration in the pET45b(+ ...
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bioRxiv - Biophysics 2019Quote: ... For lipid mobility measurements inside and outside of Gag assembly sites in mammalian cells 3×105 Jurkat T cells 72 h post-infection with NL4.3 HIV-1 Gag-iGFP virus particles or 24 h post-transfection with NL4.3 HIV-1 Gag-eGFP expressing plasmid were resuspended in 200 µL of Leibovitz’s L 15 medium (Sigma) and incubated with 25 nM of the indicated fluorescent lipid analogue probe for 5 min at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately 700nl of either pUb6-tdTOM or pUb6-Sema3a-tdTOM plasmid (2µg/ml with 1% Fast Green, Sigma) was delivered into the lateral ventricle of embryo’s brain through a beveled glass pipette applied to a Picospritzer (Parker) ...
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bioRxiv - Biochemistry 2021Quote: 100 bacterial homologs of nucleobase/cation symporter 2 (NCS2) proteins were cloned into a modified pET28 plasmid (Novagen) with either an N- or a C-terminal deca-histidine tag and a TEV protease recognition site at the central facility of the New York Consortium on Membrane Protein Structure (NYCOMPS ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmid was linearized and subjected to in vitro transcription with digoxigenin labeling (DIG RNA labeling kit, Sigma). Sections were hybridised with 500 ng/ml riboprobe in hybridization solution at 72°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmid constructs were confirmed by DNA sequencing and transformed into Escherichia coli BL21 (DE3) Rosetta2™ (Novagen®). Bacterial cells were cultured at 37°C in Terrific Broth containing 50 μg/ml kanamycin in Ultra Yield™ baffled flasks (Generon ...
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bioRxiv - Cell Biology 2021Quote: ... and/or with plasmid DNA (e.g. 1µg/well for 6-well plates) for 24 hrs using GeneJuice (Merck Millipore). siRNAs targeting USP7 ...
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bioRxiv - Cell Biology 2021Quote: ... A plasmid encoding for TrxHis-Cobl-like1-411 was generated by PCR and subcloning into pET-32 (Novagen) (primers ...
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bioRxiv - Developmental Biology 2022Quote: ... This plasmid was transformed to Novagen’s Rosetta™ 2(DE3)pLysS competent cells (Millipore Sigma, Cat#71403-3) for rhFGF10 expression ...
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bioRxiv - Cell Biology 2022Quote: ... plasmids encoding the final constructs were transformed into 20 μL of Rosetta (DE3) competent cells (EMD Millipore, 70954) and grown overnight at 37°C in 5mL LB containing 100 μg/mL Ampicillin (Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The plasmid was transiently transfected at a density of 2 × 106 cell per ml using polyethyleneimine (PEI) (Sigma) with a mass ratio of 1:4 ...
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bioRxiv - Biochemistry 2022Quote: ... The EC1-2 construct was cloned into pET21a plasmid system and transformed into BL21 (DE3) competent cells (Novagen). Cultures were grown in autoinduction media (PMID ...
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bioRxiv - Biophysics 2022Quote: ... The EC1-2 construct was cloned into pET21a plasmid system and transformed into BL21 (DE3) competent cells (Novagen). Cultures were grown in autoinduction media 24 overnight and harvested via centrifugation ...
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bioRxiv - Microbiology 2022Quote: ... Parasites containing the episomal plasmids selected with WR99210 were subsequently grown with 400 μg/ml Neomycin/G418 (Sigma) to select for integrants carrying the desired genomic modification as described previously (Birnbaum et al. ...
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bioRxiv - Immunology 2021Quote: ... calcium-phosphate-DNA-particle were generated by mixing the plasmid DNA’s with a 250 mM CaCl (Sigma-Aldrich) solution which was then added dropwise to an equal volume of 2 x hepes buffered saline and subsequently incubated for 20 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... MDCK cells were transfected with linearized plasmid DNA and selected with either 500 μg/mL G418 (Sigma-Aldrich) or 400 μg/mL Zeocin (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... TRCN0000028509 (sh-AC2) and the scramble control pLKO.1-Puro plasmid (sh-scr) were obtained from Sigma-Aldrich. The sequence of shRNAs are:
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bioRxiv - Neuroscience 2020Quote: ... CGNs (3 × 106 cells) and plasmids were suspended in 70 μl of Dulbecco’s modified Eagle’s medium (Sigma-Aldrich) and exposed to decay pulses using a CUY21-edit II pulse generator (BEX ...
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bioRxiv - Developmental Biology 2019Quote: ... HEK293 cell viability after iOn plasmids transfection was assessed by dye exclusion with Trypan blue solution (0.4%, Sigma). FP expression was either assayed by flow cytometry ...
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bioRxiv - Cell Biology 2019Quote: ... cells that had integrated plasmid were selected in media supplemented with G418 (400 µg mL-1 Sigma-Aldrich) for 10 days ...
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bioRxiv - Microbiology 2020Quote: ... GBS bearing the pFfluc-Erm plasmid must be exposed to the cofactor D-luciferin (Millipore Sigma cat. # L9504), which was prepared in a filter-sterilized stock solution at a concentration of 28 mg/mL in water ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids encoding CaMKII variants were co-transformed with lambda phosphatase into Rosetta 2(DE3)pLysS competent cells (Millipore). Expression was induced overnight with 1mM Isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Neuroscience 2020Quote: ... and cargo in a 1:4:1 ratio with PEI reagent (1:3 plasmid to PEI ratio, Sigma). Three days after transfection ...
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bioRxiv - Developmental Biology 2019Quote: ... under a stereoscope and using a glass microcapillary 1-2μl of plasmid DNAs was injected together with Fast Green (0.1%, Sigma) into different ventricles of the organoids ...
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bioRxiv - Biochemistry 2019Quote: ... the corresponding genes were amplified by PCR from S.cerevisiae genome and cloned into the plasmid pETDute-1 (Novagen). To construct N-terminal 6×His tagged Ups1-Mdm35 fusion protein ...
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bioRxiv - Cancer Biology 2020Quote: The puromycin selection gene was replaced with green fluorescent protein (eGFP) cDNA in Lentiviral pLKO.1 plasmid (Sigma). shRNA oligos (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... parasites carrying the WR99210-selected episomal plasmid were cultivated in the presence of 400 µg/ml G418 (Sigma) to select for integrants with the desired modification of the genomic locus ...
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bioRxiv - Molecular Biology 2021Quote: ... Taq pol and StuI genes were subcloned for expression in the pET24-d plasmid (Novagen, Merck Millipore, Germany) using its NcoI and XhoI restriction sites ...
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bioRxiv - Molecular Biology 2021Quote: ... Taq pol and StuI genes were subcloned for expression in the pET24-d plasmid (Novagen, Merck Millipore, Germany) using its NcoI and XhoI restriction sites ...
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bioRxiv - Molecular Biology 2021Quote: ... see Table 1 for complete plasmid list) was induced by treating cells with 2 µg/mL doxycycline (Sigma) for at least 72 hours ...
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bioRxiv - Cell Biology 2021Quote: ... transformed with pET:THMT:PTBP3 (empty plasmid from Peti and Page, 2007) at 15°C overnight with 400 μM IPTG (Sigma). Induced pellets were resuspended in low imidazole purification buffer [20 mM sodium phosphate (pH 7.4) ...
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bioRxiv - Cancer Biology 2022Quote: Plasmids for knockdown of CCL2 (TRCN0000301701 and TRCN0000301702) and CCL7 (TRCN0000317599, TRCN0000068135, and TRCN0000068136) were obtained from Sigma. shRNA control plasmid (SHC002 ...
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bioRxiv - Microbiology 2022Quote: Plasmids containing WT or mutant NS1 sequence were transfected into FreeStyle 293F cells using polyethylenimine (PEI) (40K) (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The CAG-tdtomato or CAG-tdtomato-T2A-RHEBCA plasmid (3.5μg/μl) was mixed with the dye Fast Green (0.3 mg/ml,Sigma-Aldrich) and injected (~1 μl/embryo ...