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Citations for Millipore Sigma :
6701 - 6750 of 7633 citations for Yellow Fever Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the NPSH levels in the samples (50 µg of protein) were mixture of 50 µL of 6% TCA (Sigma Aldrich). Subsequently ...
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bioRxiv - Physiology 2024Quote: ... Protein lysates were prepared from human RV tissue using modified RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Sigma). Proteins were diluted in 4x Laemmli sample buffer ...
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bioRxiv - Plant Biology 2024Quote: ... Expression of proteins was validated by performing a Western blot analysis against the FLAG-epitope tag using an anti-FLAG antibody (Sigma). For the EMSA assays ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Equal amounts of protein were separated by SDS-PAGE and transferred to polyvinylidene fluoride membranes (Immobilon-P, Millipore, Bedford, MA). Membranes were blocked with PBS plus 0.1% Tween 20 (PBST ...
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bioRxiv - Cell Biology 2024Quote: ... we used direct blue stains of total protein by saturating PVDF membranes for 10 min in 0.008% Direct Blue 71 (Sigma-Aldrich) dissolved in 40% ethanol and 10% acetic acid ...
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bioRxiv - Cancer Biology 2024Quote: ... The total ATP production was measure in nm/µg of protein by the ATP colorimetric assay kit (Sigma, MAK190-1KT) and experiment was performed as per provided manufacturer’s procedure ...
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bioRxiv - Immunology 2024Quote: ... The proteins were collected using 800 μL elution buffer (20 mM Na2PO4, 300 mM NaCl, 300 mM imidazole; Sigma Aldrich) and dialyzed against MOPS buffer (30 mM MOPS (3-morpholinopropane-1-sulfonic acid) ...
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bioRxiv - Cancer Biology 2024Quote: ... The target protein mixed with sumo protease and tag were then passed through a second 1 mL Ni-column and the flow-through solution containing target protein was collected and concentrated with an Amicon Ultra-15 centrifugal filter unit (10,000 MWCO) (EMD Millipore) at 4℃ ...
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bioRxiv - Cell Biology 2024Quote: ... and following centrifugation immunoprecipitations were done using supernatant (I mg protein) and anti-FLAG antibody (Sigma #F1804; dilution 1:1000) and Protein G agarose beads (60 µL ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration in the remaining supernatant was assessed using a Bradford reagent assay according to manufacturer’s protocol (Sigma-Aldrich - B6916), and normalized on collected fecal weight and on animal weight.
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bioRxiv - Cell Biology 2024Quote: ... HA-tagged proteins were probed with a 1:1000 dilution of Roche rat anti-HA monoclonal 3F10 primary (Sigma 11867423001) and a 1:5000 dilution of donkey anti-rat DyLight800 (Invitrogen Life Technologies SA5-10032) ...
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bioRxiv - Microbiology 2024Quote: ... mice were intranasally administered with 3 µg of the RSP protein and 5 μg of cholera toxin (CT; Sigma-Aldrich) as immunological adjuvant ...
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bioRxiv - Microbiology 2024Quote: ... The protein was finally eluted with 50 μL of 300 ng/μL 3x FLAG peptide (Sigma-Aldrich, St. Louis, MO) for 45 minutes at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Overexpression of the fusion protein was induced by adding 1 mM isopropyl-D-thiogalactopyranoside (IPTG; Sigma Aldrich; St. Louis, MO) to the transformed cell culture ...
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bioRxiv - Microbiology 2024Quote: ... 5 mg of total protein per sample was combined with 100 μL of ANTI-FLAG M2 Affinity Gel (Sigma-Aldrich) and mixed on the end-to-end rotator for 2 hours at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... and the fractions with the highest concentrations of mutant PGA proteins were concentrated into PBS using Amicon Ultra-4 Centrifugal Filters with a 10 kDa molecular weight cutoff (Merck Millipore). This process resulted in a protein with a purity exceeding 70 % and a concentration of approximately 1 mg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... The isolation of nuclear proteins was achieved with the CelLytic™ NuCLEAR™ extraction kit (Sigma Aldrich, St. Louis, MO) using the manufacturer’s recommended protocol ...
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bioRxiv - Biophysics 2024Quote: ... The protein was then concentrated to about 100 μM (Amicon Ultra-15, PLQK Ultracel-PL Membrane, 50 kDa, Merck, Millipore). After size-exclusion chromatography with a Superdex 200 Increase10/300 GL column (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... The resultant protein with an N-terminal His6-tag was concentrated using a 10 kDa Amicon Ultra-15 concentrator (Millipore). Protein concentrations were determined from the absorbance at 280 nm using a molar absorptivity constant (ε280 = 22,920 M-1 cm-1 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (EMD Millipore, IPVH00010), then blocked in Tween 0.1% Tris-buffered saline containing 5% bovine serum albumin (Tocris 5217 ...
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bioRxiv - Developmental Biology 2024Quote: Bovine OVGP1 (bOVGP1) and murine OVGP1 (mOVGP1) recombinant proteins were purified using the Amicon Pro Purification System (Merck Millipore Ltd) by combining metal chelation chromatography with an Amicon concentrator membrane ...
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bioRxiv - Molecular Biology 2024Quote: ... and 500 µl of 2 mg/ml proteins were incubated with washed anti-FLAG magnetic beads (Cat. No. M8823, Millipore) at 4 °C for 3 h on rotating wheel ...
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bioRxiv - Molecular Biology 2024Quote: Bl6 mice were immunized with 50 µg of the His-tagged LC purified protein in PBS and TiterMax (Sigma-Aldrich) adjuvant (1:10 ratio ...
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bioRxiv - Cell Biology 2024Quote: ... and screened for expression of the fusion proteins by Western Blotting with an α-FLAG M2-HRP antibody (Sigma-Aldrich).
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bioRxiv - Neuroscience 2024Quote: ... The eluted protein was concentrated with Amicon Ultra 15 centrifugal filters (Regenerated cellulose 10 000 NMWL; Merck Millipore, Darmstadt, Germany), aliquoted and flash-frozen on dry ice and stored at −80°C until use ...
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bioRxiv - Neuroscience 2024Quote: ... ck1α expression was induced for 16 hours prior to treatment with CHX to stop protein synthesis (10µg/ml) (Sigma-Aldrich). Cells were then harvested and lysed with EB2 supplemented with 5mM EDTA pH 8.0 at the times indicated after CHX addition ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 µg of protein was diluted to a concentration of 1 µg/µl and 2 µg of anti-FLAG M2 antibody (Sigma) was added and incubated overnight at 4°C for IP ...
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bioRxiv - Neuroscience 2024Quote: ... and equal amounts of proteins were separated by SDS-PAGE (10% or 4-20% gradient gels) and transferred to Immobilon-P membranes (Merck-Millipore). After chemiluminescence detection ...
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bioRxiv - Cell Biology 2024Quote: ... For 3D samples (Figs. 5f, g), protein was extracted using SDS buffer (5% SDS, 50 mM triethylammonium bicarbonate (TEABC; T7408, Sigma)) in combination with mechanical tissue disruption ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then blocked in 6% donkey serum and immunolabeled for protein gene product 9.5 (PGP9.5, 1:500, Millipore-Sigma, SAB4503057-100UG) as a pan-neuronal marker or rabbit-IgG (Abcam ...
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bioRxiv - Biophysics 2024Quote: The NF protein sequences used were adapted from Rattus norvegicus (UniProt accession P19527, P12839, F1LRZ7) and cloned into pET28a(+) (Novagen). A cysteine was introduced near the N-terminus of each sequence to enable the grafting chemistry ...
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bioRxiv - Biochemistry 2024Quote: For the ISD sample preparation each 50 μL aliquot of human CSF was resuspended in 0.2% RapiGest SF (Waters) in 50 mM ammonium bicarbonate 5 with 800 ng of yeast enolase protein (Sigma-Aldrich) added as an internal protein control ...
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bioRxiv - Physiology 2024Quote: ... Protein concentration was measured using the bichinchoninic acid (BCA, ThermoFischer #23225) method with bovine serum albumin (BSA, Sigma-Aldrich #P0834) as standard ...
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bioRxiv - Systems Biology 2024Quote: ... The cells were then incubated with 1ug/ul primary antibody (For TRUB1 staining, TRUB1 Rabbit anti-human polyclonal, 50-172-8037, Protein tech; for PUS7 staining, PUS7 Rabbit anti-human, HPA024116, Sigma) in 1% BSA/PBS-Triton (0.1% ...
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bioRxiv - Synthetic Biology 2024Quote: ... proteins (50 µg from each sample) were first reduced with 10 mM dithiothreitol (DTT) (Sigma-Aldrich, St. Louis, MO, USA) and subsequently alkylated using 50 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: 50 μg of HpARI proteins were incubated with 50 μg of Heparan Sulphate (Heparan sulfate sodium salt from bovine kidney, Sigma) in PBS for 30 min hour at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Fractions that were supposed to contain the protein of the expected size were concentrated by ultrafiltration (10 kDa, UFC9010, Millipore), and aliquots were frozen at -20 °C until use.
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bioRxiv - Developmental Biology 2024Quote: ... and contained 800 μl culture media (Leibovitz’s L15 Medium, no phenol red, Thermo Fisher 21083027; 50 ng/μl Protein A; 0.01% Methyl Cellulose, Sigma 274429). Surface conditions were critical to successful cultures ...
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bioRxiv - Plant Biology 2024Quote: ... 2.5–40 μg of total protein extracts were separated on SDS-PAGE gels and blotted onto PVDF Immobilon-P membrane (Millipore). GFP was detected using the anti-GFP antibody (Mouse monoclonal ...
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bioRxiv - Plant Biology 2020Quote: ... 1 ml of transfected protoplasts were incubated for 12 h for protein expression in the presence of 1 μM DSF (Sigma, USA), and were treated with or without 1 μM flg22 (MoonBiochem ...
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bioRxiv - Plant Biology 2019Quote: ... pulled-down proteins were separated on SDS-PAGE and analyzed by immunoblotting with anti-Flag and anti-Myc antibodies (Sigma-Aldrich).
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bioRxiv - Synthetic Biology 2021Quote: ... Extracts were then clarified at 4’000 × g for 10 min and concentrated to ~4 mg/mL total protein concentration using 3 kDa molecular weight cutoff (MWCO) Centriprep concentrators (EMD Millipore) to account for dilution during preparation ...
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bioRxiv - Cancer Biology 2021Quote: ... and nascent puromycylated proteins was detected by Western blot analysis as above using a mouse anti-puromycin antibody 12D10 (1:3000)(MABE343, Sigma-Aldrich).
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bioRxiv - Biochemistry 2020Quote: ... Free glycans were separated from protein with a 10 kDa molecular weight cutoff centrifugal filter unit (Millipore Amicon Ultra-0.5 #UFC5010). The glycan-containing filtrate was desalted with a PD minitrap G-10 column (Cytiva ...
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bioRxiv - Cancer Biology 2021Quote: ... Kinase assays were performed as described in (20) using 200ng of purified EPHA2 recombinant protein (OriGen Technologies) or indicated concentration of purified Enolase (Sigma Aldrich), in the absence or presence of about 50ng of purified Src-GFP (or Src3A-GFP as indicated ...
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bioRxiv - Cell Biology 2019Quote: ... Protein translation was analyzed on the WES automated western blotting system (ProteinSimple) using anti-Puromycin antibody (1:50; Sigma-Aldrich, MABE343).
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bioRxiv - Cell Biology 2020Quote: ... Proteins in the media fraction were precipitated by adding 0.15 ml of 100% trichloroacetic acid (TCA) (Sigma Chemical, St Louis, MO) and incubated on ice for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... and permeabilized with 0.1 % triton X-100 for 10 mins for intracellular staining with a mouse or rabbit monoclonal antibody against the target protein (1h) followed by a secondary antibody conjugated with FITC- or Alexa Fluor 647 (Sigma-Aldrich) (1.100 ...
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bioRxiv - Cell Biology 2020Quote: ... 3 mg of protein was subjected to a pre-clearing step by incubating samples with prewashed salmon sperm DNA/protein-A agarose beads (Millipore Sigma) for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... A total of 30 μg of protein from each sample were subjected to SDS-PAGE electrophoresis and transferred to PVDF membrane (Merck Millipore, USA). Then the membrane was blocked in 5% w/v bovine serum albumin (BSA ...