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Citations for Millipore Sigma :
6701 - 6750 of 10000+ citations for 6 CHLORO 4' METHYLFLAVONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... cells were cultured until confluency on a 6-well and treated with the corresponding compound (500 µM auxin (Sigma-Aldrich, I5148-2G) or 500 nM dTAG-13 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... and added dropwise to HEK293T cells that were seeded onto 10-cm dishes a day prior at a density of 5-6 × 106 cells in 10 ml of fresh DMEM with 10% heat inactivated FBS (Sigma-Aldrich #14009C). 18hrs after transfection ...
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bioRxiv - Microbiology 2022Quote: A CRISPR array containing 6 identical spacers targeting the tetR gene flanked by 7 repeats was cloned into pCDF-Duet (Novagen, Merck Millipore) by ligation after NcoI and SalI digestion ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated on 6-well cell culture-treated plates and differentiated using 100 nM phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, P1585) for 72 h in serum-free RPMI 1640 medium (SFM ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were washed several times in PBSDT and endogenous peroxidase activity was inactivated by treatment with 6%H2O2 (Sigma-Aldrich, Cat# H1009) for 1 hr at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... duttoni (NIH Rocky Mountain Laboratories, Hamilton, MT) were grown in BSK-H medium with 6% rabbit serum (Sigma Aldrich, St. Louis, MO) until the culture reached log phase (∼4 days) ...
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bioRxiv - Immunology 2024Quote: ... The minced tumor tissue was transferred to a 6-well plate containing 3 mL/well of tumor digestion media (NK cell media + 1 mg/mL collagenase IV (Sigma, cat. C5138) + 0.2 mg/mL DNAse I (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.05% Tween 20 (pH 6) at 98°C and/or treatment with bacterial type XXIV proteinase (10 μg/mL; Sigma; Catalog #P8038). The sections were blocked with 2% bovine serum albumin (VWR ...
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bioRxiv - Developmental Biology 2024Quote: ... tissue pieces (1 to 6 mm3) were equilibrated for ten minutes on ice in cryomedium consisting of 1.5M dimethylsulfoxide (DMSO, D2650, Sigma-Aldrich, Hoeilaart, Belgium), 0.15M sucrose (D21881000 ...
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bioRxiv - Cell Biology 2024Quote: ... Vessel patency and intravascular flow was confirmed between days 6-8 by addition of sterile 50µg/mL 70kDa FITC- or rhodamine-conjugated dextran (Sigma #FD70S or #R9379) to the media reservoirs ...
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bioRxiv - Immunology 2024Quote: ... cells were seeded in a 6-well plate and transduced with 10 μL of concentrated lentivirus in the presence of 10 μg/mL polybrene (Millipore-Sigma; H9268) and replaced with fresh media 24 hrs ...
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bioRxiv - Cell Biology 2023Quote: Recombination of the conditional tm1c allele was induced in utero by injecting time pregnant females with a single intraperitoneal (IP) injection of 6 mg tamoxifen (Millipore Sigma, T5648)/40 g body weight dissolved in corn oil ...
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bioRxiv - Molecular Biology 2022Quote: ... Cytokine levels in PBMC culture supernatants were measured using the MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel-Custom 6 Plex-Immunology Multiplex Assay (Millipore; #HCYTOMAG-60K). Analytes included were the following ...
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bioRxiv - Neuroscience 2023Quote: ... Digested pancreata were washed with HBSS +0.1% BSA and subjected to discontinuous density gradient using histopaque (6:5 Histopaque 1119:1077; Sigma, St. Louis, MO). The islet layer (found at the interface ...
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bioRxiv - Cell Biology 2023Quote: ... Human dermal fibroblasts were isolated and grown from 6 mm skin punch biopsies as previously described.70 Dermal fibroblasts were cultured on 0.1% gelatin (Sigma catalog number: G1890) treated wells using fibroblast media consisting of DMEM (Gibco catalog number ...
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bioRxiv - Cell Biology 2022Quote: ... to block non-specific staining and incubated with a mouse anti-acetylated tubulin antibody (Clone 6-11b-1, 1:100; Sigma Cat# T6793) for 4 h at room temperature ...
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bioRxiv - Genomics 2022Quote: The stable cell lines constantly expressing the minigenes were subjected to four time points (0, 3, 6, and 9 h) of post actinomycin D treatment (final concentration of 1 μg/mL; Sigma, no. A9415) treatment in three biological replicates ...
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bioRxiv - Biochemistry 2023Quote: ... C2C12 cells were seeded in a 6 cm dish and 105-106 virus particles were added dropwise to media containing 8 ug/mL Sequabrene (Sigma S2667-1VL) and 25 mM Hepes buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... The murine melanoma cell line B16F10 derived from a C57BL/6 mouse (ATCC, cat# CRL-6475) was purchased from ATCC and grown in DMEM (Sigma, cat# D6546) supplemented with 10% Fetal Bovine Serum (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... T8787)/PBS at 37C for 2 days and subsequently immersed in a blocking solution of 10% DMSO + 6% donkey serum (EMD Millipore: S30) + 0.2% Triton X-100/PBS at 37C for 3 days ...
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bioRxiv - Immunology 2023Quote: ... were cocultured for 6 hours with autologous herpesvirus papio-immortalized B lymphoblastoid cell lines (B-LCLs) pulsed with Nef peptides (Sigma-Aldrich Japan) at 1 μM concentration or as indicated otherwise under GolgiStop (monensin ...
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bioRxiv - Developmental Biology 2023Quote: A feeder layer of inactivated murine embryonic fibroblast cells was cultured in 6-well plates on gelatin (0.1%, Sigma-Aldrich G7041-100G) at a seeding density of 1.6 x 104 cells/cm2 in base medium consisting of DMEM (1x ...
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bioRxiv - Developmental Biology 2023Quote: ... a feeder layer of inactive murine embryonic fibroblast cells was cultured in 6-well plates coated with 0.1% gelatin (Sigma-Aldrich G7041-100G) at a density of 1.6 x 104 cells/cm2 in a base medium containing DMEM (1x ...
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bioRxiv - Molecular Biology 2023Quote: ... 6- to 12-week-old mice were orally administrated with 180mg/kg of Tamoxifen (TAM) (Sigma-Aldrich, St. Louis, MO, T5648-5G) for 5 days ...
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bioRxiv - Cancer Biology 2024Quote: ... BxPC-3 cells were transduced with retroviral particles in a 6-well plate using polybrene transfection reagent (EMD MIllipore, TR-1003-G) and incubated for 48 h at 37°C ...
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bioRxiv - Cell Biology 2024Quote: Uninduced and induced samples were treated with either 0.2 μM or 2 μM Latrunculin A in DMSO (Sigma, CAS 76343-93-6) up to 4 h at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... For some experiments the PBS was adjusted to a final concentration of 6 mg/ml of poly-L-glutamate (Sigma- Aldrich, P4761). Plasmids were stored at 4°C for short-term and -20°C for long-term storage ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were incubated at 37 °C and 5% CO2 for 24 hrs and selected with growth medium containing 6 μg/mL puromycin (Sigma-Aldrich, P8833) and 750 μg/mL neomycin (Thermo ...
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bioRxiv - Immunology 2023Quote: ... Plates were then blocked for 6 h with PBST (PBS with 0.01% Tween-20) containing 2% BSA (Sigma-Aldrich; cat. no. 10735094001) at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... double-stained cells were also stained with 10 μM Laurdan (6-dodecanoyl-N,N-dimethyl-2-naphthylamine, Sigma Aldrich, Zwijndrecht, the Netherland). Laurdan was excited at 395 nm and 470 nm emission wavelength was used for rigid membrane and 508 nm for fluid membrane environment ...
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bioRxiv - Systems Biology 2023Quote: ... and resuspended in 40 µl kinase buffer containing 300 µM ATP with 6 µCi γ-32P-ATP (Hartmann analytics) and 10 U bovine PKA (Sigma, 539576). For no PKA control ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were selected with 5 µg/ml blasticidin S and surviving colonies were isolated using 6 mm Pyrex® cloning cylinder (Sigma-Aldrich). Gene knockout was verified by western blotting of whole cell lysates with appropriate antibodies.
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bioRxiv - Genomics 2024Quote: ... neural crest cells were passaged onto fibronectin-coated 6-well plates (about 1 million cells per well) using Accutase (Sigma-Aldrich A6964) and fed with neural crest maintenance medium (1:1 ratio of DMEM-F12 and neurobasal ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK 293TACE2 and A549ACE2 cells were generated by a transduction with a lentivirus expressing human ACE2 (kind gift from Benjamin Murrel, Karolinska Institutet) in the presence of 6 μg/ml Polybrene (Sigma-Aldrich, H9268). Clones were isolated and selected for homogeneous ACE2 expression as determined by HTM ...
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bioRxiv - Immunology 2024Quote: ... IL-6 and IL-17A were assessed using the High Sensitivity T-cell Discovery Array 3-Plex (Millipore, St. Charles, MO, USA) at Eve Technologies using the Bio-Plex™ 200 system (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2024Quote: ... Pre-seeded A549 cells were subsequently transduced with the lentivirus in the presence of 6 μg/ml of polybrene (Sigma-Aldrich, TR1003). After 24 hours ...
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bioRxiv - Neuroscience 2024Quote: Zebrafish larvae at 5 and 6 dpf were embedded in 1% low-melting temperature agarose diluted into MilliQ water (Sigma-Aldrich, USA). The fish were covered with artificial cerebro- spinal fluid (ACSF ...
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bioRxiv - Physiology 2021Quote: ... were incubated at 4 °C overnight with 5 μg/mL anti-FLAG (Sigma-Aldrich, F1804) in 200 mM NaHCO3 buffer ...
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bioRxiv - Microbiology 2020Quote: ... and TIGR4 (serotype 4) were inoculated onto tryptic soy agar (TSA) (GranuCult-Millipore Burlingon MA) plates supplemented with 3% sheep blood (iTek StPaul MN ...
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bioRxiv - Cancer Biology 2021Quote: PCa cells were seeded onto the Millicell EZ SLIDE 4-well glass slides (PEZGS0496, Millipore) pre-coated with Poly-L-lysine (P4707 ...
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bioRxiv - Biochemistry 2020Quote: ... IdU containing media was replaced with fresh media containing 4 mM HU (Sigma; cat# H8627) for designated times ...
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bioRxiv - Cancer Biology 2021Quote: ... The eluted kinases (proteins) were concentrated with Amicon Ultra-4 (10K cutoff) spin columns (Millipore), purified by removing the detergent using methanol/chloroform extraction ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM EDTA) at 4 °C for 10 min before 3% of Ficoll-400 (Sigma) was added and assembled Nucleolin complexes were resolved on a 10% TBE PAGE gel at 4 °C.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then stored at +4°C until propidium iodide (PI; Sigma-Aldrich, #P4864-10ML) staining was performed ...
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bioRxiv - Cell Biology 2020Quote: ... 4-dinitrophenylhydrazine using protein carbonyl content assay kit according to the manufacturer’s instructions (Sigma, USA). The optical density of the adduct was examined at 375 nm ...
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bioRxiv - Immunology 2021Quote: Pieces of eWAT and liver (∼30 mg) were fixed in 4% formaldehyde solution (Sigma-Aldrich), paraffin-embedded ...
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bioRxiv - Genetics 2021Quote: ... Embryonic tissues or whole embryos were isolated and fixed in 4% paraformaldehyde (Sigma PFA, 158127) in PBS.
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bioRxiv - Developmental Biology 2021Quote: ... Embryonic tissues or whole embryos were isolated and fixed in 4% paraformaldehyde (Sigma PFA, 158127) in PBS.
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bioRxiv - Plant Biology 2021Quote: ... incubated with anti-Flag M2 Magnetic beads (4 °C for 2 h; Sigma, Cat# M8823), washed 5 times and then eluted with 3xFLAG peptide ([0.1 mg/mL] ...
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bioRxiv - Molecular Biology 2021Quote: ... for 30 minutes at 4°C and stained with 50 μg ml-1 PI (Sigma). Samples and results were analyzed by FACS-Aria and FlowJo (FlowJo ...