Labshake search
Citations for Millipore Sigma :
6651 - 6700 of 10000+ citations for 6 CHLORO 4' METHYLFLAVONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... 7 x105 HEK293 cells were seeded in each well of a 6-well plate and were transfected with 2 μg of respective plasmid DNA using PEI reagent (Sigma-Aldrich, 408727). After 24 hours ...
-
bioRxiv - Biochemistry 2019Quote: apoTaLPMO9A was prepared by dialyzing the purified TaLPMO9A extensively using D-tube Dialyzer Maxi (6-8 kDa) from Millipore (Billerica, MA, USA). Dialysis was performed in 20 mM phosphate buffer (pH 7 ...
-
bioRxiv - Immunology 2019Quote: ... erythrocytes were depleted by sequentially layering 10 ml PBS followed by 8 ml of 6% w/v dextran (high molecular weight, Sigma-Aldrich, in PBS) and gently inverting ...
-
bioRxiv - Genomics 2019Quote: ... and 12.5 ng/µL oligo(dT) at 42°C for 2 h in the presence of 6 ng/µL actinomycin D (Sigma, #A1410-2MG) to prevent antisense artifacts (Perocchi et al ...
-
bioRxiv - Bioengineering 2020Quote: ... and 800 mg L-1 humic acids (CAS No. 1415-93-6) were prepared by adding in ultrapure water (Sigma Aldrich, UK).
-
bioRxiv - Neuroscience 2020Quote: ... and MT+SNL mice from tissues collected on day 6 (post behavioral testing) using Tri Reagent (T9424, Sigma Aldrich, St. Louis, MO) and RNeasy Micro kit (74106 ...
-
bioRxiv - Neuroscience 2021Quote: ... iPSC-derived neuron progenitors were expanded using 6-well plates coated with 15 μg/ml Poly-L-Ornithine (Millipore Sigma Cat# P4957) and 10 μg/ml laminin (Invitrogen Cat# 230171015 ...
-
bioRxiv - Cell Biology 2021Quote: ... lentiviral particles were generated by transfecting HEK293T cells with 6 μg PLKO.1 scramble control and MISP-targeted shRNA plasmids (SIGMA; TRCN0000422523, TRCN0000116527), 4 μg psPAX2 packing plasmid (Addgene ...
-
bioRxiv - Neuroscience 2020Quote: Primary hippocampal neurons were isolated from P1 C57BL/6 mice by dissociating with 0.25% trypsin and plated onto poly-L-lysine (Sigma-Aldrich, MO, USA)-coated culture dish ...
-
bioRxiv - Neuroscience 2020Quote: ... The heads were placed in a 15 ml centrifuge tube filled with perfluoropolyether (Fomblin Y, LVAC 16/6, average molecular weight 2700, Sigma-Aldrich, U.S.A.). Data was acquired on a small animal MRI system (BioSpec 94/30 animal MRI system ...
-
bioRxiv - Immunology 2021Quote: ... Fab samples were concentrated to ~6-8 mg/ml and buffer exchanged to TBS using Amicon ultrafiltration units with a 10 kDa cutoff (EMD Millipore Sigma). Final Fab yields were ~1 mg ...
-
bioRxiv - Neuroscience 2020Quote: Different cellular and mitochondrial parameters of the glutamate- or erastin-induced cell death pathways were analyzed using the Guava easyCyte 6–2L flow cytometer (Merck Millipore, Darmstadt, Germany) upon harvesting adherent HT22 cells and following addition of different fluorescent dyes.
-
bioRxiv - Cell Biology 2021Quote: ... Cells were collected 6 days after plating in 1 ml PBS and incubated with 60-80 ul collagenase IV [10mg/ml] (Sigma #C5138-1G) at 37 C for 10 mins ...
-
bioRxiv - Cancer Biology 2022Quote: HEK293T cells at 80-90% confluency in a 12-well plate were transfected using 6 μL GeneJuice Transfection Reagent (Sigma-Aldrich, 70967) and 100 μL RPMI 1640 medium (ThermoFisher ...
-
bioRxiv - Biochemistry 2020Quote: ... cells were fixed using a 6% (v/v) formaldehyde: phosphate-buffered saline solution and stained with 1% (w/v) crystal violet (Sigma-Aldrich, Italy) in 70% (v/v ...
-
bioRxiv - Immunology 2020Quote: ... single cell suspensions were derived from bone marrows of 4-6-week-old C57BL/6 WT and Igh VH locus inversion mice and incubated in Red Blood Cell Lysing Buffer (Sigma-Aldrich, #R7757) to deplete the erythrocytes ...
-
bioRxiv - Biochemistry 2021Quote: ... Female C57BL/6 mice wild type mice (7-9 weeks) were injected intraperitoneally with etomoxir (100 µg, Sigma Aldrich, Cat. No E1905) and/or LPS (1 µg ...
-
bioRxiv - Molecular Biology 2020Quote: Confluent monolayers of HAEC grown on 6-well plate were exposed to unidirectional PSS (6, 12 and 24 hours) using a modified flow device.10 Neutralized MCDB-131 medium (Sigma Aldrich, MO) containing 7.5% sodium bicarbonate solution ...
-
bioRxiv - Microbiology 2021Quote: ... The resulting hybridoma cells were plated onto 96-well plates and cultured in HAT selection medium (Hybridoma-SFM [Life Technologies, Grand Island, CA, USA]; 10% FBS; 1 ng/mL mouse IL-6; 100 μM hypoxanthine [Sigma-Aldrich, St ...
-
bioRxiv - Neuroscience 2022Quote: ... mice aged 5 – 6 weeks and harboring the Fezf2-CreER allele were treated with 50 mg/kg body weight tamoxifen (Sigma-Aldrich,T5648) by oral gavage for 5 consecutive days ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were seeded in 6-well plates the previous day and treated either with 200ug/ml Cycloheximide (CHX) (Sigma-Aldrich # C-7698) in fresh growth media for 2 ...
-
bioRxiv - Immunology 2022Quote: ... RPMI-1640 media containing 10% FBS was supplemented with antibiotics (6 mg/l Nitrofurantoin, Sigma Aldrich, cat. no. N7878; 16 mg/l Imipenem monohydrate, Sigma Aldrich, cat ...
-
bioRxiv - Cancer Biology 2022Quote: Cell proliferation was assessed by the colorimetric assay of sulforhodamine B 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate (SRB) (Sigma Aldrich, USA)[25] ...
-
bioRxiv - Biophysics 2022Quote: The dyes used in this study and the working dilutions were: 6-Dodecanoyl-N,N-dimethyl-2-naphthylamine (LAURDAN, 4.5 μM, Sigma-Aldrich, Merck, #40227), Hoechst 33342 (80 uM ...
-
bioRxiv - Immunology 2019Quote: ... duplicate wells of a 12-well plate were seeded with 1 ml/well of the cell suspension (1 × 106 cells/ml) and virus was added in the presence of 6 μg/ml polybrene (Sigma-Aldrich, #107689) (multiplicity of infection of 25) ...
-
bioRxiv - Zoology 2019Quote: ... or cortisol treatments (8 g peanut butter, 2 g wheat germ with 6, 8, or 12 mg of cortisol [H4001, Sigma Aldrich, USA]). Dosages of 0 ...
-
bioRxiv - Zoology 2019Quote: The midgut tissue dissected out from continuous toxin-exposed susceptible and toxin exposed insects for 6 generations (n=5) were processed for RNA isolation using TRI reagent TM (Sigma Aldrich, USA) following the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2019Quote: ... Then ten half seeds with involved embryos were placed in a Petri dish containing 6 ml of distilled water with or without (±) 10 μM of ABA (Sigma Chemical Co.). The Petri dishes were then incubated in the dark at 20°C ...
-
bioRxiv - Pathology 2021Quote: ... Infected tissues were disrupted and digested overnight (12 hours) using Fast Prep® Lysing Matrix tubes containing 0.2 mg (6 U) Proteinase K (Sigma-Aldrich®) diluted 1/40 in Lifton’s Buffer (100 mM EDTA ...
-
bioRxiv - Neuroscience 2019Quote: ... filled with a thin layer of 2.5% agarose containing either pure agarose (Sigma Aldrich, cat. no.: A5093, CAS no.: 9012-36-6) or agarose plus 1.5 M sodium chloride (Sigma Aldrich ...
-
bioRxiv - Immunology 2020Quote: ... Th-1 and Th-17 cells were restimulated for 6 hr in the presence of PMA (100 ng/ml, Sigma, Cat#P8139), Ionomycin (1 μM ...
-
bioRxiv - Neuroscience 2019Quote: ... The heads were then placed in a 15 ml centrifuge tube filled with perfluoropolyether (Fomblin Y, LVAC 16/6, average molecular weight 2700, Sigma-Aldrich, U.S.A.). Samples were measured at room temperature ...
-
bioRxiv - Immunology 2019Quote: ... CHO cells pre-incubated with trivalent Strep-Tactin-SpyCatcher or buffer alone were incubated with 2 µM ATTO 647 biotin (ATTO Technology # AD 647-71) pre-mixed with 6 µM biotin (Sigma-Aldrich # B4501) for 30 minutes at 25 °C ...
-
bioRxiv - Molecular Biology 2021Quote: ... isolated of at least 6 animals per preparation were mixed with collagen type I (PureCol™ EZ Gel solution, # 5074, Sigma-Aldrich, Vienna ...
-
bioRxiv - Developmental Biology 2021Quote: Larvae at 2-6 dpf were first anesthetized with 0.03 % Ethyl 3-aminobenzoate methane sulfonate salt (Sigma-Aldrich, St. Louis, MO, USA) and pinned onto a Sylgard-filled recording chamber (I-2450 ...
-
bioRxiv - Developmental Biology 2021Quote: ... were obtained from the Developmental Studies Hybridoma Bank (DSHB), anti-acetylated microtubule antibody (6-11, b-1) was obtained from Sigma (1:200), anti-Lky (1:1000 ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were seeded in 6-well plates and 24 h later CIGB-300-F and Hoechst 33342 (Sigma, St. Louis, MO, USA) were added at a final concentration of 30 μM and 4 μg/mL ...
-
bioRxiv - Cell Biology 2019Quote: ... Lentiviral-containing supernatant was collected at 72 hours and used to transduce SAF-A-AID-mCherry cells in the presence of RPMI media supplemented with 10% FBS and 6 μg/mL polybrene (Sigma, H-9268). After 48 hours ...
-
bioRxiv - Developmental Biology 2021Quote: The proteins in the sample were precipitated using methanol/chloroform and were resuspended in 6 M Guanidine HCl (Sigma cat# G3272-1KG), buffered using 50 mM EPPS (Alfa Aesar cat# A13714) ...
-
bioRxiv - Biophysics 2021Quote: ... 3C cleavage was performed in 30 mins and the protease was separated from HsPepT2 by gel filtration using a Superose® 6 Increase 10/300 (Sigma-Aldrich). The top fraction was concentrated to 10 mg/mL using a 100 kDa cutoff concentrator (Corning Spin-X UF concentrators ...
-
bioRxiv - Cancer Biology 2021Quote: ... and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and urethane (ethyl carbamate, EC; CAS# 51-79-6) from Sigma (St. Louis, MO). Primer sets were Kras ...
-
bioRxiv - Molecular Biology 2021Quote: ... then they were brought back to room temperature and then they were alkylated using 6 µmoles of iodoacetamide (BioUltra; Sigma-Aldrich, USA) in the dark at room temperature for 30 minutes ...
-
bioRxiv - Microbiology 2020Quote: Water samples were filtered within 6 hours of sampling under gentle vacuum (∼380 mm Hg) on 0.45-µm polycarbonate filters (Millipore Isopore, Billerica, MA). Filters were immediately placed into CTAB buffer (100 mM Tris-HCl (pH 8) ...
-
bioRxiv - Immunology 2021Quote: ... BMDC clusters were harvested on day 8-10 in 5 mls of comlete I-10 media at room temperature and then stimulated at 1-2x106 cells per well of an untreated 6-well plate (Sigma M9062-100EA) in 2 mls of I-10 medium without GMCSF.
-
bioRxiv - Cell Biology 2020Quote: ... As a positive control we used a bona fide Src substrate peptide corresponding to amino acids 6-20 of p34cdc2 (Millipore-Sigma, 12-140). The reactions were carried out at room temperature in a total volume of 25 μL for 40 min in white 96-well ...
-
bioRxiv - Genetics 2019Quote: ... 0.1%SDS and 1% Tween 20 and re-stained for the loading using the anti-glucose-6-phosphate dehydrogenase antibody (Millipore, cat #A9521). Blots were again imaged on ChemiDoc MP (Bio-Rad) ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were permeabilized for one hour with 0.5 % Triton X-100 and 6 % FBS in PBS and further incubated for another hour with mouse anti-FLAG M2 (1:500; Sigma-Aldrich F1803) in PBS with 0.1 % Triton X-100 and 6 % FBS (room temperature) ...
-
bioRxiv - Biochemistry 2022Quote: ... using the absorbance measurement of 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) substrate at 405 nm (Sigma Aldrich Catalog # 11468120910).
-
bioRxiv - Cancer Biology 2022Quote: We analyzed chemotherapy responses of human leukemia cell lines expressing wild-type NT5C2 or NT5C2 mutant constructs following 72-hour incubation with increasing concentrations of 6-mercaptopurine by measuring metabolic reduction of the tetrazolium salt MTT using Cell Proliferation Kit I (Sigma-Aldrich, #11465007001) following manufacturer’s instructions.
-
bioRxiv - Genomics 2022Quote: ... culture medium was replaced with S1-media (Final 11,6 g/L MCDB131, Sigma Aldrich, M8537-1L; 2 mM D-+-Glucose, Sigma Aldrich, G7528-250G; 2,46 g/L NaHCO3, Sigma Aldrich, S5761-500G ...