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Citations for Millipore Sigma :
6601 - 6650 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Fractions containing dCas9 protein were pooled and the protein was concentrated with spin concentrators (Amicon Ultra 15, MWCO 10k; Millipore), and after adding glycerol to 20% final concentration ...
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bioRxiv - Cell Biology 2020Quote: ... protein concentration was routinely determined by densitometry of Sypro-Orange stained gels against a calibration curve with protein standards (Sigma) using ImageJ ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein extracts were then mixed with the indicated primary antibodies and protein A/G agarose (Sigma, St. Louis, MO, USA) or anti-FLAG M2 agarose for overnight at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... For the ChIP using SV40 chromatin isolated 30 minutes post-infection we substituted a mixture of protein A and protein G magnetic beads (16-663, Millipore) for the protein A and protein G agarose which we previously used in our publications ...
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bioRxiv - Cell Biology 2019Quote: ... Gene Tex) antibody was incubated with protein G coated magnetic beads (PureProteome protein G magnetic beads, LSKMAGG10, EMD Millipore Corp.) for 1-3 hrs in IP (immunoprecipitation ...
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bioRxiv - Microbiology 2019Quote: The genes of either E protein or E DIII protein (residues 301-403) of ZIKV (GZ01, KU820898) without tag were cloned into pET28a vectors (Novagen) and expressed by IPTG-induction in BL21 (RIL ...
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bioRxiv - Cell Biology 2021Quote: For conjugation of SNAP-tagged proteins (paxillin, kindlin) recombinant proteins to be labeled with fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼50 μM ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated proteins and input proteins were analyzed by immunoblotting with α-HA or α-FLAG antibodies (Sigma-Aldrich, Cat # A8592).
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bioRxiv - Microbiology 2020Quote: ... Protein-protein interactions in situ were visualized using the Duolink® in situ proximity ligation assay (PLA) system (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Proteins smaller than 30 kDa were removed by passing the protein lysate through an Amicon Ultra - 15 Centrifugal Filter (Millipore). One mg of cell lysate in 500 μL lysis buffer was pre-cleared by adding 10 μL of protein A/G PLUS-agarose beads (Santa Cruz ...
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bioRxiv - Molecular Biology 2019Quote: Purified proteins were concentrated to the indicated protein concentration described in the text using Amicon Ultra centrifugal filters (10K MWCO, Millipore) and added to solutions at varying concentrations with the indic ated final salt and molecular crowder concentrations ...
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bioRxiv - Cell Biology 2019Quote: ... Bcl2/Adenovirus E1B 19 kDa and protein-interacting protein 3-like (BNIP3L/NIX) from Sigma-Aldrich (St. Louis, MO, USA), perilipin (PLIN ...
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bioRxiv - Cell Biology 2020Quote: ... The target protein was detected by specific primary antibodies followed by secondary horseradish peroxidase (HRP)-conjugated antibodies (for less abundant proteins) (Sigma) or fluorescence-labeled secondary antibodies (for abundant antigens ...
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bioRxiv - Cell Biology 2019Quote: ... Protein complexes were pulled down by 1.5 h incubation with Protein A agarose beads (Millipore; endogenous RNA pol II IPs) or GFP-Trap®_A beads (Chromotek ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were loaded onto HisTrap column and flowthrough containing cleaved protein was pooled and concentrated (Amicon 3 K Sigma Aldrich). Proteins were loaded onto a Superdex 200 increase 10/300 GL column (Cytiva ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were probed for target proteins with specific antibodies against phosphorylated or total proteins and secondary antibodies before being developed using Immobilon chemiluminescent substrate (Millipore) and visualized using the Odyssey Infrared Imaging System (LI-COR).
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bioRxiv - Immunology 2024Quote: ... 20 μg cell proteins or 3-5 μg EVs proteins were denatured in LDS sample buffer containing 1M Dithiothreitol (DTT, Sigma) for 10 min at 70°C and loaded onto a 4-12% precast polyacrylamide gels for electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... 20 μl of clear lysates and debris were loaded onto the same 10% Bis-Tris gel for the comparison of total protein (Coomassie staining) and ubiquitinated protein levels (anti-ubiquitin antibody, Sigma, 05-944 ...
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bioRxiv - Cell Biology 2023Quote: ... Instrument performance was monitored daily using a system suitability test data consisting of a 30-minute gradient injection of 30fm BSA and 60fm of casein protein digest (both standards were trypsin digested in-house from commercial protein stocks (Sigma)) that was run before each sample ...
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bioRxiv - Neuroscience 2023Quote: ... and 50 µL was taken as protein input while the remaining lysate was pre-cleared using 100 µL or Protein G Sepharose beads (Sigma) at 4°C for 30 min ...
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bioRxiv - Physiology 2023Quote: ... Supernatants containing equal amounts of soluble proteins were mixed with 2x SDS protein sample buffer containing 100mM DTT (Sigma-Aldrich), denatured by incubating on a heat block at 98 C for 5 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... Protein STOP1 was selectively enriched from the total protein extract using Anti-FLAG® M2 Magnetic Beads (Sigma-Aldrich, M8823). Subsequently ...
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bioRxiv - Genetics 2024Quote: ... cOmplete proteinase inhibitor) to remove nonspecific-binding proteins and then HA-tagged proteins were eluted with HA peptides (Sigma-Aldrich). WT samples (not HA tagged ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was isolated from Nb-1 shoots with the Spectrum Plant Total RNA kit (Sigma-Aldrich) with on-column DNase I digestion (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: Extraction method 1 used a modified protocol with the GenElute Bacterial genomic DNA kit (Sigma-Aldrich). The pellet was resuspended in 180 μl of kit-included lysis buffer ...
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bioRxiv - Cell Biology 2019Quote: The transporter activity of MDR-1 was assessed using the MDR1 Efflux Assay Kit (ECM910, Millipore), as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Pop 9-12) (Figure 1) was isolated using the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich). DNA-purity and - quantity was assessed using a NanoDrop ND-1000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Genetics 2024Quote: ... Cell lysis took place in 1 ml of EZ-ChIP kit lysis buffer (Millipore, 17-371RF), and chromatin was sheared into approximately 1000 bp fragments via sonication using a Diagenode Pico Bioruptor ...
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bioRxiv - Systems Biology 2020Quote: ... mouse and naked mole rat fibroblasts were cultured as described earlier and treated with varying concentrations of AZC (Sigma-Aldrich). After 5 days of treatment ...
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bioRxiv - Microbiology 2021Quote: ... The primary antibodies (monoclonal α-HA rat 3F10 and monoclonal α-myc mouse 9E10, from Roche, distributed by Sigma Aldrich) were diluted 1:1000 and 1:500 respectively in PBS + 0.4 % BSA and the samples incubated for 1 hour at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... antibodies, and the secondary antibodies used included anti-rabbit (31460, Pierce), anti-mouse (31430, Pierce) and anti-rat (A9037, Sigma) HPR antibodies ...
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bioRxiv - Genomics 2020Quote: Twenty-four well plates were coated with: human or rat tail collagen type I (88 ng per well; Sigma-Aldrich), human collagen type III (88 ng per well ...
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bioRxiv - Molecular Biology 2021Quote: Type 1 diabetes was induced in male Sprague Dawley rats at 8 weeks of age via a single 55mg/kg tail vein injection of streptozotocin (STZ; Sigma), and monitored for 8 weeks ...
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bioRxiv - Neuroscience 2020Quote: Immunofluorescence was done as previously described (Yellajoshyula et al., 2017) for the following antibodies: Rat anti-MBP (Millipore, Cat# MAB386), Mouse anti-O4 (kindly provided by Dr ...
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bioRxiv - Physiology 2021Quote: ... Samples were analyzed using a rat insulin radioimmunoassay that has 100% cross-reactivity for mouse insulin (Millipore-Sigma #ri-13k). Insulin content was measured after acid-ethanol extraction using an insulin ELISA (Stellux Rodent Insulin ELISA ...
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bioRxiv - Physiology 2021Quote: ... Samples were analyzed using a rat insulin radioimmunoassay that has 100% cross-reactivity for mouse insulin (Millipore-Sigma #ri-13k). Insulin content was measured after acid-ethanol extraction using an insulin ELISA (Stellux Rodent Insulin ELISA ...
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bioRxiv - Plant Biology 2021Quote: ... LM19, LM2, JIM8 and JIM13 (PlantProbes, Leeds, UK) were used as primary antibodies and FITC–conjugated anti-rat IgG (Sigma) as secondary antibody in all cases ...
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bioRxiv - Neuroscience 2022Quote: ... rats were subcutaneously injected with desipramine (15 mg/kg) 30 min before 6-OHDA (Sigma-Aldrich, Saint Quentin-Fallavier, France) injection in order to protect the noradrenergic neurons ...
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bioRxiv - Neuroscience 2020Quote: ... One week after tracer application we deeply anesthetized the rat with urethane and performed a transcardial perfusion with 0.05M PBS followed by 4% PFA (Sigma Millipore). We harvested the brain and trigeminal ganglia ...
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bioRxiv - Neuroscience 2021Quote: ... one day after the apomorphine-induced rotation test the rats were administered daily 6 mg/kg L-DOPA (Sigma Aldrich) and 15 mg/kg benserazide HCl (purchased from the Chinese domestic market) ...
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bioRxiv - Neuroscience 2022Quote: ... cortices dissected from newborn rats (ramdom male and female) were triturated and plated into 5 cm dishes coated with 50 µg/ml PLL (Sigma) in culture medium consisting of Basal Medium Eagle (BME ...
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bioRxiv - Cell Biology 2019Quote: Tissue culture dishes or MatTek dishes (35 mm dish with 10 mm microwell) were coated with rat tail Type I collagen (Millipore) diluted to 0.5 μg/μl in PBS for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... A single intravitreal injection of ML240 or EerI to all P23H rats previously anesthetized (ST and LT: Diethyl ether, Merck Millipore, 100931 ...
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bioRxiv - Microbiology 2020Quote: A549 cells were seeded at 2 × 105 onto cover slides (13-mm diameter; Matsunami) pretreated with coating buffer containing 0.1% collagen (Type I from rat tail; Sigma-Aldrich) and 0.1% gelatin (from bovine bone ...
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bioRxiv - Microbiology 2020Quote: ... Hydrogels were washed with 50 mM HEPES at pH 7.5 and were coated with 200 µL of 0.25 mg/ml rat tail collagen I (Sigma-Aldrich; C3867) in 50 mM HEPES at pH 7.5 overnight at room temperature ...
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bioRxiv - Genomics 2021Quote: ... after which the cells were selected for 10 days on 0.1% gelatine coated 10-cm dishes in 60% Buffalo Rat Liver (BRL)-conditioned medium containing 0.8 μg/mL puromycin (Sigma P9620), 250 μg/mL G418 (ThermoFisher 11811031 ...
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bioRxiv - Neuroscience 2021Quote: ... Rats were cardiac perfused with phosphate-buffered saline (0.1 M, pH 7.4) followed by 4% paraformaldehyde (Sigma, St. Louis, MO), brains were removed ...
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bioRxiv - Biophysics 2020Quote: ... Polyclonal rabbit anti-clathrin heavy chain (Cat. Nr. SAB1410212) and rat monoclonal anti-β1-integrin (Cat. Nr. MAB1997) were from Sigma. Rabbit monoclonal anti-caveolin-1 (Cat ...
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bioRxiv - Neuroscience 2020Quote: ... and rats were perfused transcardially with 60 ml of 0.9% saline followed by 120 ml of 4% paraformaldehyde (Sigma-Aldrich). Brains were extracted and post-fixed in 4% paraformaldehyde overnight ...
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bioRxiv - Immunology 2020Quote: ... 8-µm-thick tissue sections were rehydrated and Fc-blocked with normal mouse and rat serums in the presence of 0.3% Triton X100 (Sigma-Aldrich). Tissues were stained with F4/80-APC (Clone BM8 ...