Labshake search
Citations for Millipore Sigma :
6551 - 6600 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... the pregnant rats were intraperitoneally (ip) injected with 1mL/Kg or LPS 0111:B4 (L2630; Lot# 114M4009V, Sigma-Aldrich) at a dose of 50µg/Kg and volume of 1mL/Kg diluted in sterile saline solution (0.9% NaCl solution ...
-
bioRxiv - Neuroscience 2022Quote: ... extracted rat neonates (P0) hippocampi were dissociated and plated onto 12 mm poly-D-lysine (Sigma-Aldrich, Cat. #P6407)-treated glass coverslips ...
-
bioRxiv - Neuroscience 2022Quote: ... rats were anesthetized with an intraperitoneal injection of 50 mg/kg sodium pentobarbital (Sigma-Aldrich, St. Louis, MO, USA). An incision of approximately 1 cm in length was made along the midline ...
-
bioRxiv - Neuroscience 2024Quote: ... Rats underwent bilateral infusion with a volume of 500 nl of either muscimol (400 ng/µl) (Sigma-Aldrich, M1523), gabazine (25 pmol ...
-
bioRxiv - Microbiology 2024Quote: ... Horseradish peroxidase-conjugated rabbit anti-rat IgG and horseradish peroxidase-conjugated goat anti-chicken IgY (Sigma, St. Louis, MO) were used for western blotting at a dilution of 1:80,000 ...
-
bioRxiv - Biophysics 2023Quote: ... The suspended cells in DMEM were mixed with rat tail-derived type I collagen in 0.1% acetic acid and 2X DMEM (Sigma). After neutralizing the pH with sodium hydroxide ...
-
bioRxiv - Molecular Biology 2023Quote: Hippocampal tissue from P2 Wister rats was chopped to small pieces and digested by 5mg/ml trypsin (Sigma #T1005) - 5mg/ml DNaseI for 5 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... Rat anti-Ago2 (cat no. SAB4200085) and goat anti-Apolipoprotein B (cat no. AB742) were purchased from Millipore Sigma. Rabbit anti-CD63 (cat no ...
-
bioRxiv - Cell Biology 2023Quote: ... The cDNAs encoding human AMPKα1-KD (aa 27-290) (ref. 164) and rat CaMKK2-KD42 were inserted into the pET-28a (Novagen) vectors for expressing His-tagged recombinant proteins as described previously ...
-
bioRxiv - Cell Biology 2023Quote: Coverslips with either adult mouse or neonatal rat cardiomyocytes were incubated 1h with anti-α-actinin antibody (A7811, Sigma) at room temperature ...
-
bioRxiv - Microbiology 2023Quote: ... Prior to seeding HepG2 cells, wells and coverslips were surface-coated with collagen (type I, rat tail) (Sigma-Aldrich) in a 50 µg/mL solution in Dulbecco’s phosphate-buffered saline (DPBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1-5 mm3 biopsies from naked mole-rat or shaved mouse back skin were incubated with either: I) dissociation buffer (1.25 mg/ml collagenase I (Sigma), 0.5 mg/ml collagenase II (Worthington) ...
-
bioRxiv - Microbiology 2024Quote: ... fd1-cas9 schizonts derived from infected rats were harvested after 22 hr culture and purified on a Nycodenz (Sigma) gradient ...
-
bioRxiv - Molecular Biology 2024Quote: 96-well flat-bottomed tissue culture plates (Earthox) were coated with varying concentrations of rat tail collagen I (Sigma) or fibronectin (Sigma ...
-
bioRxiv - Genomics 2019Quote: ... RNA-protein complexes were incubated with antibody-bound Protein A Dynabeads (anti-FLAG [Sigma Aldrich F3165] or anti-IgG) at 4C for 2 hours ...
-
bioRxiv - Cancer Biology 2019Quote: ... A preclearing step was made for 2hours at 4°C with 50µl of previously blocked protein-A and protein-G beads (Sigma) for 200µg of chromatin ...
-
bioRxiv - Cancer Biology 2021Quote: Protein extracts were separated from each cell line with RIPA protein lysis buffer (R0278, Sigma, St Louis, MO, USA) containing 1X protease cocktail (P8340 ...
-
bioRxiv - Biochemistry 2022Quote: ... protein induction was performed on a small scale (2mL) and cells were disrupted using BugBuster protein extraction reagent (Novagen).
-
bioRxiv - Microbiology 2021Quote: ... The cell-free supernatants were stored at –80°C for total protein quantification using the BCA protein assay (Sigma).
-
bioRxiv - Plant Biology 2021Quote: ... and immunoprecipitation of the antibody/protein complexes was performed by adding 50 μl of Protein A-Sepharose (Sigma-Aldrich) made up as a 50% (w/v ...
-
bioRxiv - Developmental Biology 2019Quote: ... The HDAC6 protein was immunoprecipitated from total protein lysates (0.25 mg) of cells treated with different compounds using protein A agarose beads (Sigma). The HDAC6 protein bound beads were used as enzyme source and was incubated with the HDAC assay buffer (50 mM Tris pH 8.0 ...
-
bioRxiv - Cell Biology 2021Quote: ... and protein diluted to 1mg/ml 1ml of protein lysate was precleared with 20μl of protein G sepharose bead slurry (Sigma) for 1 hour to remove native immunoglobulins ...
-
bioRxiv - Genomics 2021Quote: ... were captured using 40 μl of protein-A or protein-G Dynabeads (Invitrogen; pre-washed with 0.1% BSA [Fraction V, Sigma), by 4 hrs incubation time at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... The supernatant was collected and protein was estimated using the bicinchoninic acid protein estimation method (BCA, B9643, Sigma-Aldrich). We separated a protein sample (80µg ...
-
bioRxiv - Microbiology 2022Quote: ... The SPA-tagged Rho protein was visualized by hybridization with the primary mouse ANTI-FLAG M2 monoclonal antibodies (Sigma-Aldrich; dilution 1:5,000) and the secondary goat peroxidise-coupled anti-mouse IgG antibodies A2304 (Sigma-Aldrich ...
-
bioRxiv - Genomics 2021Quote: For profiling the wildtype and oncogenic KMT2A protein we used two monoclonal antibodies targeting the KMT2A N-terminus: Mouse anti-KMT2A (1:100, Millipore Cat #05-764) refered to as KMT2A-N1 ...
-
bioRxiv - Neuroscience 2020Quote: ... Dendrites were identified using a staining against the microtubule-associated-protein-2 with Rabbit-anti-MAP2 PAB (Milipore Sigma, cat. AB5622, 1:1000) and GAR-STAR488 SAB (Abberior ...
-
bioRxiv - Microbiology 2021Quote: ... followed by incubation with 1 µg/ml of a SARS-CoV nucleocapsid protein (NP) monoclonal antibody (mAb) 1C7C7 (Millipore-Sigma, St. Louis, MO) shown to cross-react with SARS-CoV-2 NP ...
-
bioRxiv - Molecular Biology 2021Quote: ... the antibody – lysate mixture was incubated at 4°C with rotation for 1 hr prior to the addition of protein A/G agarose beads (Millipore, 50 L/IP) and continued overnight incubation ...
-
bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight while shaking at 4°C in the blocking solution that contained combinations of the following primary antibodies: mouse anti- three microtubule-binding domain tau protein (3R-tau, 1:500, Millipore, Cat# 05-803), rabbit anti-purkinje cell protein 4 (PCP4 ...
-
bioRxiv - Microbiology 2022Quote: ... THP-1 cells were harvested and washed twice with PBS and lysates were prepared using CytoBuster protein extraction reagent (Novagen/EMD, Gibbstown, NJ) supplemented with complete mini EDTA-free protease inhibitor (Roche ...
-
bioRxiv - Cancer Biology 2019Quote: CAF cell monolayers were lysed for protein with RIPA buffer containing protease inhibitor cocktail at 1:100 dilution (both Sigma-Aldrich, Dorset, UK). Lysed cell suspensions were quantified for protein concentration using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... The precipitated proteins were centrifuged for 10 min at 18,000 g and the protein pellet resuspended 50 mM NH4HCO3 before digestion with sequencing grade porcine trypsin (enzyme to protein ratio of 1:40) (Sigma-Aldrich, MO, USA) for 16 hours at 37°C ...
-
bioRxiv - Genomics 2019Quote: ... Approximately 25μg of proteins were analyzed on 10% SDS-PAGE followed by western blots using monoclonal anti-FLAG-M2-Peroxidase (HRP) (Sigma Aldrich, 1:2000) and anti-β actin (1:4000).
-
bioRxiv - Biochemistry 2021Quote: ... 25 µg of total protein were separated by denaturing PAGE and subjected to Western blotting using mouse monoclonal anti-FLAG antibody (M2, Sigma Aldrich, 1:1000) followed by probing with an HRP-coupled anti-mouse light chain-specific secondary antibody (1:10000 ...
-
bioRxiv - Biochemistry 2020Quote: A 10 μM solution of purified PAPR10-CD protein was incubated for 20 minutes at 37°C with 1 mM final concentration of β-Nicotinamide Adenine Dinucleotide (β NAD+) (Millipore-Sigma) in a reaction buffer (50 mM HEPES ...
-
bioRxiv - Microbiology 2021Quote: ... followed by incubation with 1 µg/ml of a SARS-CoV nucleocapsid protein (NP) monoclonal antibody (mAb) 1C7C7 (Millipore-Sigma, St. Louis, MO) shown to cross-react with SARS-CoV-2 NP ...
-
bioRxiv - Neuroscience 2022Quote: ... The immunoprecipitated proteins were analyzed by SDS-PAGE and Western blotting using the following antibodies: anti-HA (Sigma, H3663; clone 12CA5, 1:1000), anti-Flag (Sigma ...
-
bioRxiv - Microbiology 2024Quote: ... a 10 μM solution of purified PAPR10-CD protein was incubated for 20 minutes at 37°C with 1 mM final concentration of β-Nicotinamide Adenine Dinucleotide (β NAD+) (Millipore-Sigma) in a reaction buffer (50 mM HEPES ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight while shaking at 4◦C in the blocking solution that contained combinations of the following primary antibodies: mouse anti-3 microtubule-binding domain tau protein (3R-Tau, 1:500, Millipore, Cat# 05-803), rabbit anti-Purkinje cell protein 4 (PCP4 ...
-
bioRxiv - Physiology 2023Quote: ... We blocked sections in 5% normal goat serum in phosphate-buffered saline with 0.1% Tween 20 (blocking buffer) for 30 min at room temperature (RT) and then incubated in primary antibody for laminin protein (1:500, Sigma catalog no. L93931) overnight at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... separate sets of sections were immunohistochemically processed using anti-glial fibrillary acidic protein (GFAP) monoclonal antibody 1:500 (G3893, Sigma, Saint Louis, Missouri), anti-doublecortin (DCX ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein was detected using the Simple-Western-JESS capillary western blot system (Bio Techne) multiplexed for total protein and chemiluminescence detection of mouse αSFPQ (1:2000; Sigma-Aldrich WH0006421M2, RRID:AB_1843565) or mouse αTIAL1 (1:10 ...
-
bioRxiv - Immunology 2023Quote: ... For purification of HDAC1-Flag 1 mg of protein was mixed with 120 µl of crosslinked M2-Flag beads (Sigma Aldrich, M8823-5ml) and incubated rotating overnight at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: Knockdown of proteins was achieved through RNA interference either by CaCl2 transfection of gene-specific shRNA encoding vectors (pLKO.1; Sigma-Aldrich or shRNAmiR) on DIV 7 or by transduction of cells with lentiviral particles harboring the gene-specific shRNA on DIV 2 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Serum insulin and c-peptide levels were measured by insulin or C-peptide ELISA respectively following the manufacture’s protocol (Sigma Aldrich, St. Louis, MO, USA). Mouse metabolic testing and fasting methods followed established protocols [16,33,34].
-
bioRxiv - Immunology 2020Quote: Blood was withdrawn at the indicated times during high fat diet period and plasma lipid levels were enzymatically measured using ELISA (Sigma Aldrich, Zwijndrecht, the Netherlands).
-
bioRxiv - Bioengineering 2022Quote: ... Peanut-specific IgG1 was detected using goat anti-mouse IgG1-HRP conjugated (Southern Biotechnology Associates) and substrate TMB liquid substrate system for ELISA (Sigma-Aldrich, St. Louis, MO). The plates were read in an ELISA plate reader at 405 nM (IgE ...
-
bioRxiv - Cancer Biology 2022Quote: ... cell death detection enzyme-linked immunosorbent assay PLUS (ELISA) and human anti-β-actin antibody were obtained from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Poly-ADP-ribose polymerase (PARP ...
-
bioRxiv - Cell Biology 2020Quote: ... For visualization of blue protein bands from the prestained protein ladder the anti-BLUE antibody (Anti-BLUE, 2D2-F11, Millipore) was used (Schuchner et al. ...