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Citations for Millipore Sigma :
6551 - 6600 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The MTF1 protein eluent was collected and concentrated using 10 kDa MW cut-off Amicon ultra-centrifugal filter (Merck Millipore) and stored at -80°C ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was then concentrated to ~200 μL with a 100 kDa molecular weight cutoff (MWCO) Amicon Ultracentrifugal filter (Millipore Sigma) at 1000 ×g ...
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bioRxiv - Bioengineering 2022Quote: ... a known quantity of EV protein was mixed with 1% ultra-low gelling temperature agarose (Sigma Aldrich, Oakville, ON, Canada), 1% gelatin (Sigma Aldrich ...
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bioRxiv - Bioengineering 2022Quote: MSC derived small EVs were characterized for protein concentration using the bicinchoninic acid (BCA) assay (Sigma Aldrich, Oakville, ON, Canada). Nanoparticle tracking analysis was performed using ZetaView (Particle Metrix ...
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bioRxiv - Biochemistry 2022Quote: ... PTP-tagged or MHTAP-tagged protein variants were detected using the peroxidase-anti-peroxidase soluble complex (PAP) (1:2000, Sigma) as previously described.10 Detection of 6x His-tagged recombinant POLIB variants was performed with primary antibody Penta•His (1:1000 ...
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bioRxiv - Biophysics 2022Quote: ... The pre-cleared lysates were then incubated with 100 µL of antibody/protein A complexed beads (anti-FLAG [mouse, Sigma, F1804] or anti-HA [mouse ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: The nitrocellulose membrane was imaged for total protein and then blocked for one hour in blocking buffer (5% w/v bovine serum albumin [Sigma, SKU A7906] in TBS-T buffer [50mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... Lysates were assayed for phospho proteins using the Milliplex Cell Signaling kit (NFκB Magnetic Bead Signaling 6-plex, Millipore Sigma) for detection of NFκB pSer536 ...
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bioRxiv - Immunology 2022Quote: ... an aliquot of the sample was taken for measuring the total protein concentration using a BCA assay kit (Merck Millipore). The remaining sample was heat-treated (70 °C ...
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bioRxiv - Genetics 2022Quote: ... then the rest was split into two tubes with addition of 30 μl prewashed Protein A Agarose beads (Sigma, 11134515001), 3 μg anti-RAD51 (PHYTOAB ...
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bioRxiv - Genetics 2022Quote: ... Equal amounts of protein (40 µg) were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred onto Immobilon-P membranes (Millipore), as previously described (Nakamori et al. ...
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Optimization and deoptimization of codons in SARS-CoV-2 and the implications for vaccine developmentbioRxiv - Evolutionary Biology 2022Quote: ... About 2×106 cells of HEK293T cells were transfected with 2 ug of plasmids encoding S proteins using polyetherimide (PEI; Sigma). After 16 hours of incubation ...
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bioRxiv - Plant Biology 2022Quote: ... 1 mL of filtered protein extract was mixed with 40μL of anti-FLAG M2 magnetic beads (Merck, formerly Sigma-Aldrich) (equilibrated in GTEN + 0.1 % Tween-20 prior to use ...
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bioRxiv - Plant Biology 2022Quote: ... Protein samples were concentrated by centrifugation using spin columns (MW cut off of 10 kDa) (cat number UFC501096– Merck Millipore). Protein concentration was estimated by UV using calculated extinction coefficients (42,400 mol/l-1.cm-1) ...
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bioRxiv - Microbiology 2022Quote: ... or drug free tissue homogenate and extraction was performed by adding 200 µL of acetonitrile/methanol 50/50 protein precipitation solvent containing 10 ng/mL Verapamil (Sigma). Extracts were vortexed for 5 minutes and centrifuged at 4000 RPM for 5 minutes ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were separated in a 10% SDS-polyacrylamide gel and transferred to Immobilon NC membranes (Millipore Merck, Darmstadt, Germany). The membranes were blocked with 5% non-fat dry milk in PBS for 1 h at room temperature and then incubated with primary antibodies (DB 1:2000 ...
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bioRxiv - Microbiology 2022Quote: ... The polyclonal anti-Hcp antibody was developed after immunization of six-week-old female BALB/c mice with three subcutaneous injections of the purified His-Hcp protein emulsified in Freund adjuvant (Sigma).
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bioRxiv - Molecular Biology 2022Quote: Recombinant proteins used for biochemical assays were expressed from pMal-RT plasmids (see above and Table S3. For each protein preparation, E. coli Rossetta2 CapR cells (Novagen) containing freshly transformed expression constructs were plated on LB agar containing ampicillin (100 µg/mL ...
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bioRxiv - Microbiology 2022Quote: ... Sizes were only referenced according to the Gel Filtration Makers Kit (for protein molecular weight 29 kDa to 700 kDa, Sigma).
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bioRxiv - Neuroscience 2022Quote: ... using rabbit antisera to the microglial protein Iba1 (1:1000; Fujifilm Wako Pure Chemical Corp) and AlexaFluor Donkey anti-rabbit 594 (1:1000; Millipore). Slides were cover slipped with Vectashield containing DAPI (Vector laboratories).
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bioRxiv - Pathology 2022Quote: ... Fifty microgram of protein was separated by SDS electrophoresis using 10% polyacrylamide gel and transferred to an Immobilon PVDF membrane (Millipore). Membrane was blocked with 5% non-fat milk in TBST for 2 hrs and probed overnight at 4°C with the primary antibody against TIM-1/KIM-1/HAVCR (#AF1817 ...
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bioRxiv - Plant Biology 2022Quote: ... SDS-PAGE/Western blot analysis was used to identify proteins in the sample with use of anti-FLAG M2 antibody (Sigma) and anti-MYC antibody (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2022Quote: ... and the double phosphorylated form of S6 ribosomal protein (p-Ser240-244 -S6rp) were simultaneously detected through incubation with combined polyclonal goat anti-ChAT (1:500, #AB144P, Millipore) and polyclonal rabbit anti-p-Ser240-244 -S6rp (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Thirty μg of protein sample was run at 120V to separate proteins based on their molecular weights before electrophoretic transfer onto Polyvinylidene Difluoride (PVDF) transfer membranes (Millipore). PVDF membranes containing separated protein samples were blocked for one hour to prevent non-specific primary antibody binding using a solution of 5% skimmed milk ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysate collection and RNA immunoprecipitations were performed using the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore, #17-700) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were digested sequentially with Lys-C and trypsin on Microcon-10 filters (Merck Millipore, #MRCPRT010 Ultracel YM-10). The mixture was centrifuged at 1000 rcf for 5 min ...
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bioRxiv - Plant Biology 2023Quote: ... Total proteins were separated on 10% SDS-PAGE and (semi-dry) blotted onto Polyvinylidene fluoride (Immobilon-P™, Millipore®) membranes.
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bioRxiv - Plant Biology 2023Quote: ... 1.4 ml of the remaining filtered total protein extract was mixed with 30 μl of anti-c-Myc agarose beads (A7470, SIGMA) and incubated end over end for 90 minutes at 4 ºC ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were separated on a 10% SDS-PAGE gel and Western blotting was performed using anti-FLAG M2 (Sigma F3165) and anti-GAPDH (Sigma A9521).
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bioRxiv - Neuroscience 2022Quote: ... Protein samples were diluted to equivalent volumes containing 20 μg of protein and boiled in an equal volume of Laemli SDS boiling buffer (Sigma) for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... Protein samples (40 μg) were separated by 4-10% SDS-PAGE and transferred to a PVDF membrane (Immobilon-P, Millipore). Membrane was stained with anti-OXPHOS (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... The beads-antibody-protein mix was incubated overnight at 4°C and bounds protein were eluted with 35 μg HA peptides diluted in PBS (Sigma); then Laemmli buffer was added and the eluate heated at 95°C 2 min.
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bioRxiv - Molecular Biology 2022Quote: ... the eluted protein is pooled and concentrated to ∼40 mg/mL by using a 50 kDa cutoff ultrafiltration unit (Millipore). Finally ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein pellets were resuspended in 10 μl of H2O and 0.3 μl of Benzonase (≥250 units/μl, Sigma, E1014)/sample and the samples were incubated in RT for 5 min ...
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bioRxiv - Biochemistry 2022Quote: ... The first elution peak containing both proteins were pooled and concentrated to 10-15 mg/ml using Amicon Ultra Centrifugal Filter Units (Millipore) with 10-kDa molecular weight cutoffs.
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bioRxiv - Plant Biology 2022Quote: ... 1mL of the supernatant was filtered through a syringe with an IC Millex-LG 13-mm mounted 0.2-mm low protein binding hydrophilic millipore (polytetrafluoroethylene [PTFE]) membrane filter (Millipore Ireland). The KTI in solution was separated on an Agilent 1260 Infinity series (Agilent Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... Golgi apparatus was detected with mouse anti-Golgi 58K protein /formiminotransferase cyclodeaminase (FTCD) antibody (G2404, SIGMA-Aldrich, Prague, Czech Republic). The following secondary antibodies were used for detection ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 μl of the soluble protein sample were mixed with 10 μl of UPS2 spike-in (Sigma-Aldrich, cat# UPS2) diluted in 250 μl 1.25× protein loading buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were analysed by standard Western blotting procedures using an HRP conjugated secondary antibody and Immobilon chemiluminescence substrate (Millipore-Sigma). Membranes were imaged on an ChemidocTM MP (Biorad ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were analysed by standard Western blotting procedures using an HRP conjugated secondary antibody and Immobilon chemiluminescence substrate (Millipore-Sigma). Membranes were imaged on an ChemidocTM MP (Biorad ...
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bioRxiv - Molecular Biology 2024Quote: ... either 200-300 μg of cleared protein extract lysed in RIPA buffer was incubated with anti-FLAG M2 Magnetic Beads (Sigma) for 1 hour at 4 °C with rotation followed by 3 washes with RIPA buffer or 200-300 μg or protein extract was incubated with 2-5 μg of Y69 N-terminal c-MYC ...
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bioRxiv - Molecular Biology 2024Quote: Full length IMP2 protein was expressed as a pET-45 6-Histidine tagged 3C protease cleavable protein in Rosetta (DE3) pLysS Escherichia coli (Novagen) cells at 18 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... 15N] triple-labeled proteins for NMR assignments at pH 5.0 were expressed in M9 minimal medium prepared using 100% D2O (Sigma-Aldrich) as solvent (M9 D2O medium) ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein samples were subjected to SDS-polyacrylamide gel electrophoresis and transferred to a PVDF membrane (Sigma-Aldrich). Membranes were blocked with 5% milk in TBST for 1 h and incubated with antibodies as listed in Table S1 ...
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bioRxiv - Biochemistry 2024Quote: ... Imidazole was removed from the purified protein by serial buffer exchange in 10 kDa MWCO spin columns (Millipore Sigma, UFC801096). For CD44-Fc constructs ...
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bioRxiv - Biochemistry 2024Quote: ... Each sgRNA (30 ng/ul) was complexed with enhanced specificity (eSP) Cas9 protein (50 ng/ul from Millipore-Sigma (58) and individually validated for causing chromosome breaks in mouse zygotes ...
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bioRxiv - Biochemistry 2024Quote: ... Each sgRNA (30 ng/ul) was complexed with enhanced specificity (eSP) Cas9 protein (50 ng/ul from Millipore-Sigma (58) and individually validated for causing chromosome breaks in mouse zygotes ...
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bioRxiv - Microbiology 2023Quote: ... Cells were harvested by centrifugation and lysed by incubation for 20 min in BugBuster protein extraction reagent supplemented with Benzonase nuclease (Millipore) and an EDTA free protease inhibitor cocktail (Roche) ...
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bioRxiv - Cell Biology 2024Quote: ... The hexahistidine-tagged protein was expressed in Hi5 insect cells (Trichoplusia ni) grown in 1 liter of M3 medium (Sigma) supplemented with 10% fetal bovine serum (Wisent) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Eluted protein complexes were concentrated to 400 μL in an Amicon® Ultra-15 Centrifugal Filter Unit (Millipore, Burlington, MA) and further subjected to a size exclusion chromatography through a Superdex 200 Increase 10/300 column (GE Healthcare Life Sciences ...