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Citations for Millipore Sigma :
6451 - 6500 of 10000+ citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated at 37 °C and 5% CO2 for 4 days before addition of resazurin (Sigma Aldrich) to a final concentration of 20 μg/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... gonads were dissected from 4 and 5 day old female jellyfish in 400 µM menthol in seawater (Sigma-Aldrich, #M2772 ...
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bioRxiv - Bioengineering 2022Quote: ... the medium was changed to RPMI + B27-I with 5 μM Wnt inhibitor IWP-4 (SML1114, Sigma-Aldrich, USA) and incubated for 48 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... hiPSCs were plated at a density of 4×104 to 5×104 cells/cm2 onto a human fibronectin (Millipore)-coated 12-well plate in GK15 medium (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... followed by a 4-hour decalcification in the same fixative with addition of 5 % EDTA (ethylenediaminetetraacetic acid, Sigma-Aldrich) at 4 °C.
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bioRxiv - Neuroscience 2021Quote: ... Dehydrated tissue was then bleached with gentle shaking at 4°C for 48 hours in 1.5 ml of 5% H2O2 (Sigma) in methanol (1 volume of 30% H2O2 in 5 volumes of 100% methanol ...
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bioRxiv - Microbiology 2021Quote: ... centrifuged for 5 min at 16,000 g at 4°C and homogenized with glass beads (≤ 106 μm; G4649, SIGMA) in 350 μl of lysis buffer (RNeasy Plus Mini Kit ...
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bioRxiv - Cancer Biology 2020Quote: ... Conditioned media were retrieved by spinning down at 2,000 × g for 5 minutes at 4 °C then supernatants were passed through 0.22 μm filter (Millipore: SLGP033RS).
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bioRxiv - Immunology 2022Quote: ... overnight at 37°C in 5% CO2 tissue culture incubator and were subsequently fixed with 4% paraformaldehyde (Sigma-Aldrich) in DPBS for 30 minutes at RT ...
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bioRxiv - Molecular Biology 2019Quote: ... 1000 rpm continuous shaking at 4 °C) with 100 μL elution buffer (6.7 mM m6A 5-monophosphate sodium salt [Sigma # M2780-10MG] ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular ions were extracted from dried pellets by overnight incubation at 4°C in 5 ml milliRho water (Millipore). Na+ and K+ were measured by flame spectroscopy with a Solaar spectrometer.
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bioRxiv - Immunology 2019Quote: ... Cells were blocked overnight at 4°C with 150µl blocking solution per well (5% (v/v) human serum (Sigma), 5% (v/v ...
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bioRxiv - Immunology 2020Quote: ... 1 mL of lentiviral supernatant was added to 5×105 RAW 264.7 macrophages with 4 μg/mL of hexadimethrine bromide (Millipore) and cells were spinoculated at 300 x g for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 4 °C), resuspended in 5 ml lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5ξCellLytic B (Sigma-Aldrich), 1 % Triton X-100 ...
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bioRxiv - Neuroscience 2023Quote: ... Immunoprecipitation was carried out by rotating sonicated lysates overnight at 4 C with NFIA antibody (5 mg, Sigma, HPA006111) or SOX9 antibody (EMD Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... Excess TMT reagent was quenched by the addition of 4 µl of an aqueous 5% hydroxylamine solution (Sigma, 438227). Peptides were reconstituted in 0.1 % formic acid ...
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bioRxiv - Systems Biology 2022Quote: ... The lysate was centrifuged at 4,000 xg for 5 min at 4°C and the protein pelletwas resuspended in Digestion Buffer(10% 2,2,2-Trifluoroethanol (Sigma; #96924)) in 100 mM HEPEs pH 8.5) ...
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bioRxiv - Cell Biology 2022Quote: ... Excess TMT reagent was quenched by the addition of 4 μl of an aqueous 5 % hydroxylamine solution (Sigma, 438227). Peptides were reconstituted in 0.1 % formic acid ...
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bioRxiv - Cancer Biology 2024Quote: ... Isolated MLECs were treated with increasing concentration (0.1, 5 and 50 μM) of 4-hydroxytamoxifen (OH-tamoxifen) (Millipore-Sigma) for 24 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Isolated MLECs were treated with increasing concentration (0.1, 5 and 50 μM) of 4-hydroxytamoxifen (OH-tamoxifen) (Millipore-Sigma) for 24 h ...
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bioRxiv - Cell Biology 2023Quote: ... pelleted by centrifugation (5 min, 400 gavg, 4°C) and washed in phosphate buffered saline (PBS, Sigma, D8537-500ml), before being pelleted again ...
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bioRxiv - Biochemistry 2024Quote: ... Transferred PVDF membranes were blocked in 5% milk powder in 0.2% Tween-TBS overnight at 4°C and immunoblotted with mouse anti-FLAG-HRP (Sigma) conjugated primary antibody ...
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bioRxiv - Synthetic Biology 2024Quote: ... washed once with TBS pH 8.0 for 5 min and incubated for overnight at 4 °C in primary antibody (Mouse-anti-FLAG M2 [Sigma #F1804 ...
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bioRxiv - Cell Biology 2023Quote: ... gel samples were prepared by boiling for 5 min in 2x SDS sample buffer (125 mM Tris, 4% SDS, 20% glycerol, 0.04% bromophenol blue, pH 6.78) supplemented with 5% β-mercaptoethanol (Sigma). Samples were briefly centrifuged prior to loading onto 4-12% NuPAGE Bis-Tris mini-gels (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2023Quote: Undifferentiated shCTRL and shCPOX-4 Cells seeded at 0.5M cells/ml and treated with 5 μl 10mM GSK343 (Sigma, catalog no ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was cleared by centrifugation at 16,000 g for 5 min at 4 °C before it was immobilized on anti-FLAG (Sigma) agarose bead slurry for 30 min at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and then incubated overnight at 4°C with primary antibodies diluted in blocking solution [5% Goat Serum (Sigma Aldrich) + 0.2% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Excess TMT reagent was quenched by the addition of 4 µl of an aqueous 5% hydroxylamine solution (Sigma, #438227). Peptides were reconstituted in 0.1 % formic acid and mixed to achieve a 1:1 ratio across all TMT-channels ...
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bioRxiv - Cell Biology 2023Quote: ... Excess TMT reagent was quenched by the addition of 4 µl of an aqueous 5% hydroxylamine solution (Sigma, 438227). Peptides were reconstituted in 0.1 % formic acid and equal volumes were mixed ...
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bioRxiv - Microbiology 2023Quote: ... Excess TMT reagent was quenched by the addition of 4 µl of an aqueous 5% hydroxylamine solution (Sigma, 438227). Peptides were reconstituted in 0.1% formic acid and equal volumes were mixed ...
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bioRxiv - Physiology 2024Quote: ... WT and Gw females (4 months of age) underwent superovulation by intraperitoneal (IP) injection of 5 IU pregnant mare’s serum gonadotrophin (PMSG; Sigma Cat.# G4877-1000 IU ...
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bioRxiv - Cancer Biology 2024Quote: ... Beads were re-suspended in antibody buffer (50 μL) with BG4 (∼5 μM, 4 μL/sample) or H3K27ac antibody (0.5 μL/sample, Millipore) and incubated at 4 ◦C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... 5 minutes each) prior to incubating in blocking solution (4% BSA in PBS with 0.33% Tween-20, Sigma-Aldrich) at room temperature for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei pellets were then collected by centrifuge for 4 min at 1,300 × g at 4°C and washed once in PBS supplemented with 5 mM sodium butyrate (Sigma) before binding to prepared concanavalin A coated magnetic beads (Bangs Laboratories ...
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bioRxiv - Developmental Biology 2024Quote: ... at 4 x 105 cells/well on 24-well plate (Trueline) coated with 5 μg/ml fibronectin (Sigma-Aldrich). Cells were treated with either 0.01% DMSO or different concentrations of Ki8751 inhibitor for 6 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... 60 µl elution buffer (2 M urea, 50 mM Tris-HCl pH 7.5 and 5 μg/mL Trypsin (SIGMA; T6567-5×20UG)) (twice as the bead volume ...
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bioRxiv - Neuroscience 2023Quote: ... pH 10.4, 1 mM MgCl2, 1 mM ZnCl2 containing 1 mg/mL 4-nitrophenyl phosphate disodium salt hexahydrate, Sigma) were added to 20 μL of the conditioned medium ...
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bioRxiv - Neuroscience 2023Quote: ... pH 10.4, 1 mM MgCl2, 1 mM ZnCl2 containing 1 mg/mL 4-nitrophenyl phosphate disodium salt hexahydrate, Sigma) were added to 20 μL of the conditioned medium ...
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bioRxiv - Immunology 2024Quote: Gonadal white adipose tissue was digested into pre-warmed 2 mg/ml BSA 2% FBS RPMI supplemented with 2 mg/ml collagenase type II (Sigma, C6885) and placed under shaking at 180 rpm for 40 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were activated with interleukin-2 (IL-2) 450 UI/mL (Proleukin, Novartis pharma) and polybrene 2 µg/mL (Sigma-Aldrich) during at least 2 h before infection.
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bioRxiv - Neuroscience 2019Quote: Complete Freund’s Adjuvant (CFA, 20 µl, 2% in 1:1 saline) (Sigma-Aldrich, F5881) was injected into the hindpaw of mice ...
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bioRxiv - Developmental Biology 2022Quote: ... and diphospho-ERK-1 and ERK-2 (1:4000; clone MAPK-YT; Sigma; RRID:AB_477245). In our hands ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were picked up in a 1:1 mixture of 2% methylcellulose (Sigma-Aldrich) and 2.3 M sucrose ...
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bioRxiv - Physiology 2019Quote: ... 1% penicillin-streptomycin and 2 μg.ml−1 puromycin dihydrochloride (Sigma, St. Louis, MO, USA) were added the next day ...
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bioRxiv - Plant Biology 2021Quote: ... 2 g L-1 Phytagel and 1.5 g L-1 activated charcoal (Sigma-Aldrich). After inoculation ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 mM MgCl2) with 1% SDS and 1:2,000 Benzonase® nuclease (Sigma E1014). For tissue samples ...
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bioRxiv - Neuroscience 2022Quote: ... the supernatant was mixed 1:1 with RIPA buffer with 2% sarkosyl (Sigma L9150) and incubated for 1 hour at room temperature with shaking ...
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bioRxiv - Microbiology 2022Quote: ... and then 2 ml overlay comprising 1:1 viral growth medium: 2.4% cellulose (Sigma) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 mM MgCl2) with 1% SDS and 1:2,000 Benzonase® nuclease (Sigma E1014). Cell debris was removed by centrifugation at 16,000x g for 5 minutes at room temperature and protein concentration was measured using A280 values determined by Cytation 5 (BioTek) ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM MgCl2 and 1 mM EGTA) containing 2 µM unlabeled phalloidin (Sigma, #P2141) and 10 µM taxol (Sigma-Aldrich ...