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Citations for Millipore Sigma :
601 - 650 of 7419 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Mycoplasma contamination of cells was tested and ruled out with the LookOut Mycoplasma Detection kit (Sigma-Aldrich; catalog number: MP0035).
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were stained either for ALP activity using an ALP detection kit according to the manufacturer’s instructions (Sigma-Aldrich) or with crystal violet ...
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bioRxiv - Plant Biology 2022Quote: ... 3-6 µg total protein was derivatized with DNPH and subsequently detected with an anti-DNP antibody according to the manufacturer’s instruction of the OxyBlot Protein Oxidation Detection Kit (S7150, Sigma-Aldrich). Equal amounts of the derivatized protein samples were employed as loading control on a separate SDS-gel and stained with Coomassie or silver staining.
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bioRxiv - Developmental Biology 2023Quote: ... The resultant samples were load on SDS-PAGE and subjected to pIMAGO phospho-protein detection kit with fluor-680 (Sigma) detection kit and LI-COR Odyssey platform for western blot analyses of phosphorylated proteins (Millipore Sigma) ...
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bioRxiv - Plant Biology 2024Quote: ... Unique integration of the cassette in the mutants was then controlled by Southern blot using DIG-High Prime DNA Labelling and Detection Starter Kit I (Sigma). Primers used for these experiments are listed in Table S1 ...
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bioRxiv - Genomics 2023Quote: ... Cells were validated by STR analysis and regularly confirmed to be mycoplasma free by Venor GeM Mycoplasma Detection Kit (Milipore Sigma). 293T cells were cultured at 37 C ...
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bioRxiv - Biophysics 2024Quote: ... The slides were then rinsed with PBS (3×) and then stained with an in situ cell death detection (FITC-TUNEL) staining kit (Millipore), an AlexaFluor-647 anti-collagen antibody (NovusBio ...
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bioRxiv - Neuroscience 2019Quote: ... Amplification and label probe binding was performed after further washes with wash buffer A with the amplification reaction mixture containing Phi29 polymerase and the fluorophore-labeled detection oligo prepared according to the manufacturer’s recommendations (Duolink Detection reagents Red, Sigma) in a prewarmed humidified chamber at 37 °C for 100 min ...
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bioRxiv - Cell Biology 2023Quote: ... for 30 minutes at RT and permeabilized for 15 minutes at RT with 0.2% Triton 100 X buffer (Sigma). Calu-3 cells were fixed with 4% PFA for 20 minutes at RT and permeabilized for 15 minutes at RT with 0.2% Triton 100X buffer ...
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bioRxiv - Physiology 2020Quote: ... miR-150fl/Cdh5(PAC)-iCreERT2 mice were injected intraperitoneally with 100 µL of 5 mg/mL tamoxifen (Sigma, cat. no. T5648) or vehicle (12.5% vol/vol ethanol in peanut oil ...
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bioRxiv - Cancer Biology 2019Quote: ... mice were treated via tail vein injection with 80 μg of control miRNA or 5-FdU-miR-15a packaged using in vivo-jetPEI (Polyplus Transfection, Illkirch, France) or with 50 mg/kg of gemcitabine (Sigma Aldrich) via intraperitoneal injection (IP injection) ...
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bioRxiv - Molecular Biology 2023Quote: ... with either the wild type Cacna2d2-3’UTR or the Cacna2d2 3’UTR bearing mutations in the miR-423-5p binding sites (Cacna2d2 mut 3’UTR) in HEK 293 cells (Millipore Sigma). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA for reverse transcription polymerase chain reaction (RT-PCR) was isolated from 100 mg of oat leaves using TRI REAGENT® (Sigma-Aldrich, St. Louis, MO, USA) according to the provided protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We purified the PCR product using the GenElute Plasmid Miniprep Kit (Sigma Aldrich) and used it as a template to insert the mutation into OmpF— transformed with pkD46 ...
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bioRxiv - Microbiology 2021Quote: ... The amplified cDNA was purified with a GenElute PCR cleanup kit (Sigma-Aldrich), and concentration ...
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bioRxiv - Genomics 2022Quote: ... mm9) ampli?ed by the PCR DIG Probe Synthesis Kit (Sigma-Aldrich, 11636090910). The hybridized probe was immunodetected with antidigoxigenin Fab fragments conjugated to alkaline phosphatase (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... PCR was performed using the KOD Hot Start DNA polymerase kit (Novagen, TOYOBO). The list of primers used for RT PCR is indicated on table 3.
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bioRxiv - Developmental Biology 2021Quote: ... Genomic DNA was extracted using the REDExtract-N-Amp Tissue PCR Kit (Sigma): 50 µl of extraction solution and 12.5 µl of tissue preparation solution was added to each embryo ...
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bioRxiv - Molecular Biology 2020Quote: ... and PCR on extracted DNA (GenElute Mammalian Genomic DNA Miniprep Kit; Sigma-Aldrich). Selected clones were finally adapted to growth in suspension in Expi293 Expression Medium (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... The amplified cDNA was purified with the GenElute PCR cleanup kit (Sigma-Aldrich), and the quality and quantity of ds-cDNA was determined with NanoDrop 1000 and a 2100 Bioanalyzer using the Agilent DNA 7500 kit (Agilent Technologies) ...
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bioRxiv - Physiology 2022Quote: PCR reactions were performed with the KAPA3G Plant kit (Sigma Aldrich, MO, USA) under the following conditions ...
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bioRxiv - Physiology 2023Quote: ... DNA extraction was done using REDExtract-N-Amp Tissue PCR kit (XNAT, Sigma). For the PCR reaction ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: ... was washed 3 times with PBS and processed using the CelLytic MEM protein extraction kit from Sigma. Briefly ...
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bioRxiv - Pathology 2021Quote: ... followed by ligation and amplification with Duolink detection reagent Texas Red according to the manufacturer’s instructions (Duolink In Situ detection reagent-Red, Sigma Aldrich). The samples were then imaged in a confocal laser scanning microscope (Leica TCS SP5 ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were washed two times with PBS and incubated for two hours at RT with corresponding secondary antibodies and subsequently incubated for 30 min at RT with DAPI (Sigma). Stained sections were washed two times with PBS and mounted with either Fluorsave (Chemicon ...
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bioRxiv - Cell Biology 2021Quote: ... followed by detection using chemiluminescent substrates from Millipore. LAS4000 (Fujufilm-GE ...
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bioRxiv - Cell Biology 2020Quote: ... Chemiluminescence detection was performed using Immobilon reagent (Millipore) and the Gel-Doc™ XR+ system (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... and Detection Reagent Red were purchased from Sigma. PLA was performed on HEK293T cells as previously described (29) ...
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bioRxiv - Cell Biology 2020Quote: ... Duolink In Situ Detection Reagents Red (SIGMA, DUO92008); Duolink In Situ Detection Reagents Brightfield (SIGMA ...
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bioRxiv - Molecular Biology 2020Quote: ... amplification using Duolink In Situ Detection Reagents (Sigma) was performed at 37 °C for 100 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Detection was performed using Immobilion Western (Millipore #WBKLS0500).
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bioRxiv - Molecular Biology 2020Quote: ... before detection using HRP-conjugated Secondary Antibody (Sigma) and ECL Western Blotting substrate (Pierce ...
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bioRxiv - Cancer Biology 2019Quote: ... and chemiluminescent HRP detection (substrate: Millipore Sigma #WBLUF0100). Blocking was done overnight at 4°C using 5% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... Duolink In Situ Detection Reagents Brightfield (SIGMA, DUO92012).
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bioRxiv - Neuroscience 2022Quote: ... Signals were developed using ECL-plus detection (Millipore). Images were acquired on X-ray films (Denville Scientific) ...
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bioRxiv - Pathology 2023Quote: ... Signals were visualized with chemiluminescence detection reagent (Sigma) on a Chemidoc (Biorad ...
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bioRxiv - Pathology 2023Quote: ... Signals were visualized with chemiluminescence detection reagent (Sigma) on a Chemidoc (Biorad ...
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bioRxiv - Plant Biology 2024Quote: ... anti- mouse antibody for HRP detection (Sigma-Aldrich), and anti-rat antibody for HRP detection (Sigma- Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... were used for ECL detection Reagents (Sigma-Aldrich) and scanning on the Amersham Imager 600 Instrument (GE Healthcare) ...
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bioRxiv - Cancer Biology 2021Quote: To detect apoptosis in the cells after bergamottin treatment In Situ cell death detection kit from Sigma-Aldrich ((St. Louis, MO) was used ...
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bioRxiv - Cell Biology 2020Quote: ... Cell death was evaluated by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (ApopTag Plus Peroxidase In Situ Apoptosis Detection Kit, Millipore-Sigma). Cell proliferation was evaluated by Ki-67 immunostaining (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2021Quote: ... were obtained using terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay (82) (based on in Situ Cell Death Detection Kit, 11684795910, red fluorescence; Sigma-Aldrich) on sections derived from rd1 and C3H retinal explant cultures ...
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bioRxiv - Cell Biology 2021Quote: ... The TUNEL assay was performed on the testis sections (5 μm) with ApopTag plus peroxidase in situ apoptosis detection kit (Millipore, S7101).
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bioRxiv - Cell Biology 2020Quote: ... Medium was changed every 24 h and plates were stained for alkaline phosphatase activity to assure the efficiency of reprogramming (AP detection kit, Sigma-Aldrich). Once colonies were picked ...
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bioRxiv - Microbiology 2021Quote: MCDK cell viability following exposure to GO-AgNP supernatants or vehicle was assessed using a Cytotoxicity Detection Kit (Millipore Sigma #11644793001). Prior to testing ...
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bioRxiv - Neuroscience 2022Quote: ... The paraffin sections were assayed for apoptotic cells using the ApopTag Plus Fluorescein in Situ Apoptosis Detection Kit (Millipore, Cat. #S7111) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The ApopTag Peroxidase In Situ Apoptosis Detection Kit was used for Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (Sigma Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: TUNEL assays were performed after secondary antibody staining by using the In Situ Cell Death Detection Kit TMR red (Millipore #12156792910). Embryos were incubated for 4 hours at room temperature (RT ...
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bioRxiv - Cancer Biology 2023Quote: C6 cells were treated with indicated nanoformulations for fixed time points followed by Annexin V/ propidium iodide (PI) staining using Annexin V-FITC Apoptosis Detection Kit II (Cat # PF032, Sigma-Aldrich), as per manufacturer protocol ...
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bioRxiv - Molecular Biology 2024Quote: The proximity ligation assay (PLA) was conducted to determine potential protein–protein interactions using the Duolink in situ detection kit (Sigma-Aldrich) in accordance with the manufacturer’s instructions ...