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Citations for Millipore Sigma :
701 - 750 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Genotypes of the offspring were verified by PCR amplification of genomic ear DNA using the commercially available REDExtract-N-Amp™ Tissue PCR Kit (#R4775; Sigma-Aldrich, Germany) and primers designed to identify the presence or absence of the knock-in allele ...
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bioRxiv - Synthetic Biology 2021Quote: ... Green fluorescence images were used for the detection of sfGFP and red fluorescence images were used for the detection of the sulforhodamine B dye (230162, Sigma-Aldrich), used to detect the correct flow of inputs.
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bioRxiv - Cancer Biology 2020Quote: Reverse transcription (RT) reactions were carried out using 1 µg of RNA using Transcriptor RT enzyme (Catalog No. 03531287001) from Sigma Aldrich. IR minigene PCRs were performed as previously reported ...
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bioRxiv - Neuroscience 2024Quote: ... for 10 min at RT and blocked for 30 min at RT in 1% bovine serum albumin (BSA, A9647, Sigma-Aldrich). For overnight incubation at +4°C the following primary antibodies were used ...
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bioRxiv - Microbiology 2020Quote: ... The linear constructs were gel purified using a PCR cleanup kit (Sigma-Aldrich, Australia), confirmed by Sanger sequencing and concentrated to ∼500 ng/μl.
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bioRxiv - Microbiology 2019Quote: ... The digests were then purified with either GenElute PCR Clean-Up Kit (Sigma-Aldrich) or QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... DNA from tail tips was isolated using Extract-N-Amp Tissue PCR kit (Sigma) and PCR genotyping undertaken as described (The Jackson Laboratory ...
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bioRxiv - Neuroscience 2021Quote: ... PCR was performed with a commercial kit (Sigma JumpStart™ Taq ReadyMix™, P2893) and 1-μL of purified DNA ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines tested negative for mycoplasma using LookOut Mycoplasma PCR Kit (Sigma, MP0035). All cells were maintained at 37°C in a humidified incubator with 5% CO2 and were grown in media supplemented with 10% dialyzed fetal bovine serum (Sigma-Aldrich F0392) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Annealed fragments were purified using the GenEluteTM PCR Clean-Up Kit (Sigma Aldrich, NA1020). Bi-directional precursor construct (pZ073 ...
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bioRxiv - Plant Biology 2024Quote: ... DNA was extracted using the REDExtract-N-Amp Tissue PCR Kit protocol (Sigma-R4775) according to the manufacturer’s instruction ...
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bioRxiv - Genetics 2023Quote: ... Amplification of the tagmented DNA was performed using the KAPA2G Robust PCR kit (Sigma) and custom P5 and P7 indexing primers ...
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bioRxiv - Developmental Biology 2022Quote: ... DNA was purified with RedExtract-N-Amp tissue PCR kit (XNAT-1000RXN, Sigma-Aldrich) using directions provided by the manufacturer ...
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bioRxiv - Cell Biology 2019Quote: ... to quantify protein vicinity in HeLa cells and in neurons on coverslips as indicated in figures were performed using the Duolink In Situ Detection Reagents Orange kit (Sigma-Aldrich, DUO92007). Cells were fixed ...
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bioRxiv - Genomics 2021Quote: ... Alkaline phosphatase testing for the assessment of pluripotency before and after auxin treatment was performed using the Alkaline Phosphatase Detection Kit (EMD Millipore, SCR004).
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bioRxiv - Developmental Biology 2020Quote: Terminal deoxynucleotide transferase (TdT)-mediated dUTP nick end labeling (TUNEL) was performed on frozen retinal cryosections using the ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit (Millipore, Billerica, MA). TUNEL staining was performed according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: The in-situ interaction of NICD and active β-catenin was determined using the Duolink™ II secondary antibodies and detection kits (Sigma–Aldrich ...
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Multiple steps mediate ventricular layer attrition to form the adult mouse spinal cord central canalbioRxiv - Developmental Biology 2019Quote: ... TUNEL assay was performed on 20 μm thick cryosections using ApopTag® Peroxidase In Situ Apoptosis Detection Kit (Merck Millipore, Cat. # S7100) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... cells were fixed for 1 min in 4% formaldehyde and stained for AP activity using the Alkaline Phosphatase Detection Kit (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Biochemistry 2019Quote: The detection of apoptotic cells was based on the nick-end (TUNEL) labelling method using the ApopTag® in situ detection kit (S7100, Merck Millipore, USA), according to the manufacturing protocol.
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bioRxiv - Cancer Biology 2019Quote: ... TUNEL staining was performed on deparaffinized sections to detect late-stage apoptotic cells using the ApopTag peroxidase in situ apoptosis detection kit (Millipore, Burlington, MA), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining was performed using an ApopTag® In Situ Peroxidase Detection Kit (S7100, Merck Millipore, USA) according to the manufacturer’s recommendations and as previously described (36) ...
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bioRxiv - Cancer Biology 2020Quote: ... and processed on ABI 3500Xl Genetic Analyzer and mycoplasma testing was conducted with the Venor™ GeM Mycoplasma Detection Kit (Cat# MP0025-1kt, Sigma-Aldrich) both were done as recent as 1/9/2020 ...
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bioRxiv - Cell Biology 2021Quote: ... No contamination of mycoplasma was tested in every batch using the VenorGeM Mycoplasma Detection Kit (SIGMA-Aldrich MP-0025, St.Louis MO, USA), following the procedure indicated by the provider ...
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bioRxiv - Cancer Biology 2020Quote: Detection of an interaction between BRCA1 and the cavin or CAV1 proteins was assessed using the Duolink™ II Detection Kit (Sigma Aldrich) according to the manufacturer’s specifications ...
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bioRxiv - Cancer Biology 2021Quote: Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was performed using the ApopTag® Peroxidase in situ Apoptosis Detection Kit (EMD Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Terminal deoxynucleotide transferase (TdT)-mediated dUTP nick end labeling (TUNEL) was performed on frozen retinal cryosections using the ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit (Millipore, Billerica, MA). TUNEL staining was performed according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: The number of apoptotic cells were determined using the In Situ Cell Death Detection Kit (Sigma-Aldrich, 11684795910, St. Louis, MO, USA) in paraffin-embedded tissue sections ...
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bioRxiv - Physiology 2020Quote: ... TUNEL staining was performed using a protocol modified from the ApopTag peroxidase in situ apoptosis detection kit (Merck-Millipore, Cat no. S7100). Paraffin sections of adipose tissues were prepared as above ...
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bioRxiv - Microbiology 2022Quote: ... terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining of paraffin-embedded sagittal sections was performed using Apoptag Peroxidase In Situ Apoptosis Detection Kit (EMD Millipore, S7100), per the manufacturers’ instructions ...
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bioRxiv - Pathology 2023Quote: The SA-βgal activity was assessed in RPE cells at the end of the experiment using the Senescence Detection Kit (Sigma-Aldrich, #CS0030) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... rolling circle amplification, and detection (120 minutes, 37°C) were performed using the Duolink® In Situ Detection Reagents Red (cat#DUO92008, Sigma-Aldrich). Nuclei were counterstained with Hoechst 33342 ...
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bioRxiv - Cancer Biology 2019Quote: ... for primary cells miR-E shRNA viruses were concentrated to increase transduction efficiency using Centriprep Centrifugal filter YM-50 (Millipore, Billerica, MA, USA).
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bioRxiv - Cell Biology 2021Quote: ... Duolink In Situ Detection Reagents Green (DUO92014, Sigma-Aldrich) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... Duolink in-situ Detection Reagents Red (Sigma-Aldrich # DUO92008). After completion of IFA or PLA ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were visualized using chemiluminescence detection reagent (ECL, Millipore). Protein levels in each solubility fraction were quantified densitometrically and expressed as percentage of the total level (i.e ...
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bioRxiv - Developmental Biology 2019Quote: ... Detection was via alkaline phosphatase-conjugated anti-digoxygenin (Sigma), followed by NBT/BCIP color reaction (Boehringer Mannheim ...
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bioRxiv - Biochemistry 2019Quote: ... Antibodies used for detection: 4G10 Platinum anti-phosphotyrosine (Millipore), anti-pERK (E-4 ...
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bioRxiv - Immunology 2020Quote: ... was used following detection with TMB substrate (Millipore, USA). ELISA of both sera and recombinant scFv-Fc human antibodies applied with AP-conjugated anti-human IgG (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2021Quote: ... was used following detection with TMB substrate (Millipore, USA). ELISA of both sera and recombinant human antibodies was applied with AP-conjugated Donkey anti-human IgG (Jackson ImmunoResearch ...
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bioRxiv - Plant Biology 2024Quote: ... and anti-rat antibody for HRP detection (Sigma- Aldrich) were employed ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were detected by ECL chemiluminescent detection system (Millipore) in Fusion FX (Vilber Lourmat).
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bioRxiv - Cancer Biology 2024Quote: ... and In Situ Detection Reagents Red (Sigma-Aldrich, DUO92008) following the Duolink PLA Fluorescence protocol ...
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bioRxiv - Biochemistry 2023Quote: ... chemiluminescence detection was then performed using Immobilon reagent (Millipore), imaged using a ChemiDoc XRS+ imager (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... The Luminata Crescendo Millipore immunoblotting detection system (Millipore, WBLUR0500) was used to detect proteins with horseradish peroxidase-conjugated anti-rabbit secondary antibodies (Sigma ...
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bioRxiv - Immunology 2022Quote: ... and analyzed using ECL immunoblotting detection reagents (Millipore, USA) on a chemiluminescence instrument (Sage Creation ...
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bioRxiv - Developmental Biology 2023Quote: ... Antibody detection reactions were developed by enhanced chemiluminescence (Millipore).
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bioRxiv - Cell Biology 2023Quote: ... for detection of tubulin (DM1a antibody, Sigma-Aldrich, mouse) and of PTMs (tyrosinated tubulin ...
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bioRxiv - Biophysics 2023Quote: Duolink In Situ Detection Reagents Red (Sigma-Aldrich; DUO92008) was used to verify the proximity of two antibody locations in tissue samples ...
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bioRxiv - Cell Biology 2024Quote: ... Chemiluminescence detection (Immobilon Western Chemiluminescent HRP substrate, Millipore#WBKLS0500) was performed using a GE Amersham AI600 imager ...