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Citations for Millipore Sigma :
601 - 650 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... mouse anti-spike (Cat#ZMS1076, Sigma Aldrich), mouse anti-Strep (Qiagen ...
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bioRxiv - Plant Biology 2019Quote: ... 500-1200 nM primers mix (desalt purification, all provided by Sigma-Aldrich Chimie S.a.r.l France, Tables 1 and 2). qPCR mixtures were dispensed by SmartChip Multisample Nanodispenser (MSND ...
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bioRxiv - Microbiology 2021Quote: Drosophila Schneider line 2 (S2) cells were maintained and passaged at 25°C in Shields and Sang M3 insect medium (Sigma-Aldrich) supplemented with 10% fetal bovine serum.
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bioRxiv - Biochemistry 2020Quote: ... HIS (Sigma-Aldrich H3667), purified full-length Vn (1 μM in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Anti-His (Millipore, USA), anti-GST (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... or anti-His (Novagen) antibodies to detect Ub conjugates.
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bioRxiv - Plant Biology 2020Quote: ... and anti-His (Sigma) antibodies.
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bioRxiv - Plant Biology 2020Quote: ... and anti-His (Sigma) antibodies at a 1:2000 dilution ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-His (Sigma, H1029) 1:2000 ...
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bioRxiv - Plant Biology 2022Quote: ... anti-His (Sigma H1029), anti-GFP (Agrisera ...
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bioRxiv - Cell Biology 2023Quote: ... anti-HA tag (CST, 2367, 1:1000 for WB) and anti-β-actin (Sigma, 2228, 1:2000 for WB) antibodies followed by incubation with HRP-conjugated secondary antibodies (CST ...
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bioRxiv - Neuroscience 2019Quote: ... MAP2 (HM-2, mAb, 1:1000, Sigma, Cat # M4403); generic phospho-tyrosine (4G10 ...
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bioRxiv - Neuroscience 2021Quote: ... 4’,6-diamidino-2-phenylindole (DAPI; 1:1000; Sigma) was added to the cell suspension ...
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bioRxiv - Neuroscience 2021Quote: ... clone W0-2 (MABN10, Sigma-Aldrich, dilution 1:1000) primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... 6-diamidino-2-phenylindole (DAPI) (1:1000, from Millipore) for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... 4’,6-diamidino-2-phenylindole (DAPI, 1:1000, Sigma) was used to show nuclei clear and dyed for 5 min ...
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bioRxiv - Immunology 2020Quote: ... F(ab’)2 fragment (1:1000, SAB4600082 Sigma-Aldrich) for 45 min ...
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bioRxiv - Genetics 2020Quote: ... MAP2 antibody (Sigma-Aldrich, HM-2; 1:1000 dilution) was incubated for four hours ...
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bioRxiv - Cell Biology 2023Quote: ... 2.7 x 106 S2 cells (S2-DGRC) were transfected with total of 500ng of the indicated DNA constructs using dimethyldioctadecylammonium bromide (Sigma; Han, 1996) at 250µg/ml in 6-well plates ...
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bioRxiv - Cell Biology 2023Quote: ... 3.5 x 106 S2 cells (S2-DGRC) were transfected with a total of 500 ng of indicated DNA using dimethyldioctadecylammonium bromide (Sigma; Han, 1996) at 250 mg/ml in six-well plates ...
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bioRxiv - Cell Biology 2021Quote: ... FLAG-tag (Sigma F3165), Actin (Sigma A2066) ...
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bioRxiv - Cell Biology 2021Quote: ... Flag-tag (M2, Sigma), TOP2A (M042-3 ...
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bioRxiv - Neuroscience 2021Quote: ... FLAG-tag (Sigma F3165), Tubulin (Sigma T6557) ...
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bioRxiv - Neuroscience 2020Quote: Flies were dissected in S2 insect medium (Sigma #S0146). Dissected brains were moved through 6 wells (12ηl/well ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR primers were synthesized by Sigma Aldrich (S2 Table), longer DNA fragments and genes were ordered from GeneScript (S3 Table) ...
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bioRxiv - Cell Biology 2021Quote: ... anti-Rpb1-S2-P clone 3E10 (rat, monoclonal, Millipore), anti-Rpb1-S5-P clone H14 (mouse ...
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bioRxiv - Neuroscience 2024Quote: S2 cells were cultured in Schneider’s Drosophila Medium (Sigma) containing 10% heat-inactivated fetal bovine serum (FBS) ...
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bioRxiv - Biophysics 2019Quote: ... GFP-PRC1 isoform 2 proteins were expressed via BL21(DE3) Rosetta Escherichia coli cells (Novagen). Full length PRC1 was expressed for 4 hours at 18 °C after induction with 0.5mM IPTG ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were expressed in Escherichia coli strain BL21(DE3) or Rosetta 2 (Novagen, Darmstadt, Germany) and cells were lysed with a cell disruptor (Avestin ...
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bioRxiv - Developmental Biology 2021Quote: ... 250–1000 embryos were collected and transferred into a Petridish containing Pronase E protease solution (Sigma-Aldrich, P5147-1G). When 20–30% embryos were hatched ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were treated with indicated concentrations of Aristrochic Acid (AA, Sigma-Aldrich). Dimethyl sulfoxide (DMSO ...
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bioRxiv - Microbiology 2020Quote: ... the 12 aa region with AcGFP homology was ordered from Sigma-Aldrich as oligonucleotides ...
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Macrodomain Mac1 of SARS-CoV-2 Nonstructural Protein 3 Hydrolyzes Diverse ADP-ribosylated SubstratesbioRxiv - Biochemistry 2023Quote: ... and histone H3 peptide (aa 1-21) were purchased from Sigma Aldrich. Cholera Toxin A subunit (CTA ...
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Volumetric Compression Shifts Rho GTPase Balance and Induces Mechanobiological Cell State TransitionbioRxiv - Biophysics 2023Quote: ... A gel premix was made of 250 μL 40% acrylamide (AA) (Sigma) and 125 μL 2% bis-acrylamide (bis-AA ...
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bioRxiv - Molecular Biology 2023Quote: ... Embryos were then transferred to fresh KSOM+AA media (Sigma, MR-101) and cultured until the early blastocyst stage ...
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bioRxiv - Molecular Biology 2023Quote: Blastocyst-stage embryos were cultured in KSOM+AA medium (Sigma, MR-101) and treated with 200 nM of RapaLink-1 to induce diapause in vitro.
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bioRxiv - Microbiology 2019Quote: ... Bound His-tagged proteins were detected using HRP-conjugated anti-His antibodies (Sigma) at a 1:500 dilution ...
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bioRxiv - Microbiology 2021Quote: ... Borosilicate glass capillaries are loaded with a concentrated SARS-CoV-2 suspension previously coloured by the addition of 10% (V/V) of 0.5% phenol red in PBS (Sigma), then connected to a FemtoJet 4i microinjector (Eppendorf) ...
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bioRxiv - Neuroscience 2020Quote: ... double immunohistochemistry was performed using primary antibodies for myelin/oligodendrocyte glycoprotein (MOG, Sigma-Aldrich, SAB1406138) and neurofilament (NF ...
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bioRxiv - Cell Biology 2019Quote: ... Blocking was performed with a 1% solution of glycoprotein free Bovine Serum Albumin (Sigma - A3059) in PBS for 1 h at room temperature ...
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bioRxiv - Immunology 2022Quote: 10ug recombinant LCMV glycoprotein in PBS was diluted 1:1 in Sigma Adjuvant System (Sigma) and administered i.p ...
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bioRxiv - Developmental Biology 2019Quote: ... Tadpoles were treated with 50 µg.mL−1 lipopolysaccharides (LPS) from E coli 0111:B4 purified by phenol extraction (Sigma; 5 mg.L−1 in water stock) with untreated control for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... standard media was exchanged for serum-free media and cells were stimulated with LPS (E. coli LPS O55:B5, Sigma-Aldrich, St. Louis, MO, USA) at either 0.1 µg/mL ...
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bioRxiv - Immunology 2020Quote: ... were purchased from Japan SLC (Hamamatsu, Japan) and used for this study. LPS (E. coli 0111:B4) was purchased from Sigma-Aldrich (St. Louis, MO, USA) to use for LPS preconditioning ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM tris (2-carboxyethyl) phosphine (TCEP) and P8849 Protease Inhibitor Cocktail for His-tagged proteins (Sigma). The lysate was cleared at 25.000 × g for 30 min at 4°C and the supernatant was loaded on Protino®Ni-TED 2000 packed columns and purified as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Biochemistry 2020Quote: ... and E,E-FPP from the corresponding allylic alcohols (Sigma-Aldrich, MO) was performed as previously described for substrates and substrate analogs of the enzyme (+)-limonene synthase.21 ...
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bioRxiv - Molecular Biology 2021Quote: MBL protein levels were determined by SDS-PAGE followed by western blotting with Strep-tag II monoclonal antibodies (at a 1:1000 dilution from 200 μg/ml solution) (Novagen) and immunoglobulin G-alkaline phosphatase conjugates (at a 1:3000 dilution) ...
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bioRxiv - Cell Biology 2020Quote: ... For H&E (Sigma) staining ...
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bioRxiv - Developmental Biology 2021Quote: ... Elastase (Sigma, E-0258) was diluted to 0.4 mg/ml in fresh cell dissociation buffer once discs were ready ...
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bioRxiv - Genetics 2021Quote: ... E-64 (Sigma; E3132), 10 μM Leupeptin hemisulfate) ...