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Citations for Millipore Sigma :
751 - 800 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... coli Rosetta (Novagen), transformed with pGEX-ICP22-P1 ...
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bioRxiv - Biochemistry 2023Quote: ... coli cells (Millipore) and grown overnight at 37 °C and then transferred into 100 ml Luria Broth media supplemented with 100 μg/ml Kanamycin pre-warmed to 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Coli (Sigma Aldrich) and protein expression was induced with 100uM IPTG overnight at 18 °C ...
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bioRxiv - Microbiology 2024Quote: ... coli (Millipore Sigma) were transformed by heat shock with the Tsr-LBD-pet-30a(+ ...
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bioRxiv - Biophysics 2024Quote: ... coli cells (Novagen) grown in LB media at 37°C ...
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bioRxiv - Biophysics 2024Quote: ... coli (Millipore Sigma), following manufacturers’ instructions ...
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bioRxiv - Plant Biology 2024Quote: ... coli strain (Novagen). Transformed E ...
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bioRxiv - Biochemistry 2024Quote: ... coli (Novagen, 69450) co-expressing lambda protein phosphatase and purified with the QIAexpress Ni-NTA Protein Purification System (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... coli cells (Novagen). 6HIS-KIN-A2-309 (pBA2519 ...
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bioRxiv - Biochemistry 2024Quote: ... coli cells (Novagen) and purified in the absence of reducing agents ...
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6-Phosphogluconolactonase is critical for the efficient functioning of the pentose phosphate pathwaybioRxiv - Biochemistry 2024Quote: ... coli (Sigma-Aldrich). The reaction mix consisted of 5 mM G6P ...
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bioRxiv - Microbiology 2024Quote: ... coli cells (Novagen) and subsequently purified using the protocol for the wild-type protein (61).
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bioRxiv - Biophysics 2019Quote: ... His-EmGFP-DBNL and his-mCherry-DBNL were diluted in PBS (D8537, Sigma-Aldrich) to 1 nM each and 30 µl of mixed sample solution was placed on top of a cleaned glass cover-slip for measurement ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Microbiology 2022Quote: ... gonorrhoeae growth on GC-IsoVitaleX agar plates. Estrone (i. e., E) and 17-α-hydroxyprogesterone (i. e., P2) (both from Sigma) were solubilized in 2-BtOH ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed then sequentially incubated with anti-SARS-CoV-2 CR3022 antibody (Yuan et al., 2020) (1 μg/mL) and a HRP-conjugated goat anti-human IgG (Sigma) in PBS supplemented with 0.1% (w/v ...
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bioRxiv - Cancer Biology 2019Quote: ... rabbit anti-His (Sigma Aldrich), rabbit anti-Tubulin polyclonal antibody (Cell Signaling Technology).
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bioRxiv - Molecular Biology 2021Quote: ... the expression vector was transformed into Rosetta 2 (DE3) pLysS Escherichia coli (Novagen, Madison, WI, USA). An E ...
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bioRxiv - Immunology 2021Quote: ... mice received an intraperitoneal injection of either 2 mg/kg LPS (Escherichia coli 026:B6, Sigma) in PBS or PBS alone ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli were also grown in 2 mL Overnight Express Instant TB media (Sigma Aldrich, 71491-5) with added 100 μg/mL ampicillin ...
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bioRxiv - Plant Biology 2023Quote: ... Individual expression constructs were subsequently transformed into Escherichia coli Rosetta™ 2 (DE3)pLysS cells (Novagen) for heterologous expression ...
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bioRxiv - Neuroscience 2022Quote: ... and then digested in 2.5 % trypsin (Gibco)and 1200 U/μl DNase I (Sigma Aldrich) for 23 min at 37°C in a 5 % CO2 incubator ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit polyclonal anti-GLURδ1/2 (1:1000; Millipore, Cat#AB2285), rat monoclonal anti-HA (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... and 4’,6-diamidino-2-phenylindole (DAPI; 1:1000; Sigma) for nuclei counterstaining ...
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bioRxiv - Bioengineering 2023Quote: ... 6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich, 10236276001, 1:1000) in 0.1 M PBS for 15 min followed by a single wash for 10 min in 0.1 M PBS ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti Centrin-2 (1:1000, Sigma Aldrich 04-1624), mouse anti γ-tubulin (GTU88 ...
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bioRxiv - Microbiology 2023Quote: ... 4′,6-diamidino-2-phenylindole (DAPI; 1:1000; Sigma-Aldrich) was used for nuclei staining and added to the secondary antibody solution ...
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bioRxiv - Developmental Biology 2023Quote: ... 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (1:1000; Sigma) was used as nuclear counterstaining ...
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bioRxiv - Cancer Biology 2020Quote: ... The pETDuet-1-His-BAP1-ULD+ASXL2-AB protein complex was expressed in Rosetta 2 (DE3) pLysS (Millipore). The bacteria bearing the desired plasmids were propagated with aeration at 37°C in 1L of 2YT to an A600 absorbance of approximately 0.6 ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was isolated and combined with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) His Bind resin (Novagen), equilibrated in lysis buffer and incubated for 1 hour at 4°C with gentle rotation ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 mM β-mercaptoethanol prior to application of the protein to His-Select Cobalt affinity gel (Sigma-Aldrich). The slurry was rotated for 60 min prior to application to a gravity flow column ...
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bioRxiv - Biophysics 2020Quote: The recombinant MdmX (18-111 aa) was cloned into pET46Ek/LIC vector (Novagen) and expressed in E.coli BL21 (DE3 ...
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bioRxiv - Biophysics 2020Quote: The recombinant human Mdm2 (1-125 aa) were cloned into pET11a vector (Novagen) and expressed in E ...
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bioRxiv - Neuroscience 2021Quote: ... 10 μM AA and 100 nM of Retinoic Acid (RA, Sigma, #R2625-50MG). At Day 4 ...
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bioRxiv - Developmental Biology 2019Quote: ... KSOM-AA drops were always covered with a layer of mineral oil (Sigma) to prevent desiccation ...
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bioRxiv - Microbiology 2020Quote: ... Synthesized sequence of VP35 IID (aa 215-340) was cloned in pET45b (Novagen) to make His-tagged VP35 IID ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... PDI (P7496) and UFL1 (HPA030560; aa sequence 301-389) were from Sigma-Aldrich. Mouse mAbs to GCP4 (IgG1 ...
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bioRxiv - Molecular Biology 2024Quote: ... and purified using a 0.45-μm filter (Millex-AA; Merck Millipore, Darmstadt, Germany). The filtered viral particles were centrifuged at 8,000× g for 35 min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 9-aminoacridine (9-AA) and ethanol were purchased from Sigma Aldrich (Taufkirchen, Germany); 2,5-dihydroxybenzoic acid (DHB ...
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bioRxiv - Neuroscience 2024Quote: ... The air is expelled into a filtered (Millipore AA 0.22 μm syringe filter) safety container (not shown in Figure 1).
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bioRxiv - Biophysics 2024Quote: ... The embryos were cultured in EmbryoMax KSOM +AA with D-Glucose (Millipore, USA) covered with mineral oil on a glass bottom dish (35-mm ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were mounted on glass microscopy slides with DAPI-containing mounting medium (4’,6-Diamidino-2-phenylindole dihydrochloride, Vectashield-H-1200-10, Sigma-Aldrich). All brain slices were imaged by a confocal microscope (LSM 710 ...
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bioRxiv - Cell Biology 2021Quote: Drosophila S2 cells were cultured in Schneider’s medium (Sch, Sigma) supplemented with 10% insect tested fetal bovine serum (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... S2 cells were stained with Phalloidin-TRITC 1: 4000 (Sigma) for 20 min to detect F-actin ...
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bioRxiv - Genetics 2022Quote: Drosophila S2 cells were cultured in Schneider’s medium (Sigma, S0146) containing 10%-20% fetal bovine serum (Gemini ...
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bioRxiv - Biochemistry 2023Quote: ... or a VSVG epitope tag on the N-terminal tag of H2A (Sigma, V4888) and a fluorescently labelled secondary antibody (Cell Signaling ...
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bioRxiv - Cancer Biology 2021Quote: ... Flag-tag (Sigma, F3165-.2MG), Veriblot IgG (Abcam ...
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bioRxiv - Genomics 2019Quote: ... Flag-tag M2 (F1804, Sigma), goat IgG control (sc-2028 ...
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bioRxiv - Microbiology 2019Quote: ... Flag-tag (Sigma-Aldrich, 1;1000) and β-actin (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... Anti-FLAG tag (Sigma, #F1365), anti-PGK1 (Thermo # PA5-28612) ...