Labshake search
Citations for Millipore Sigma :
601 - 650 of 10000+ citations for Potassium 3 4 difluorophenyl trifluoroborate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... and two pools of S2 peptides (pools 3 and 4) (final concentration of 2μg/mL) on IPVH-membrane plates (Millipore) coated with 5μg/mL anti-mouse IFNγ (AN18) ...
-
bioRxiv - Developmental Biology 2020Quote: ... were post-fixed with 4% para-formaldehyde and blocked with PBS solution containing 0.3% Triton-X 100 and 3% normal Donkey serum (Sigma). Antibody dilution buffer consisted of PBS containing 1% BSA and 0.3% Triton-X 100 ...
-
bioRxiv - Immunology 2021Quote: ... in conjunction with pLKO.1 lentiviral vector targeting GFP or human c-Rel at a ratio of (1.5:3:4) using X-tremeGENE HP DNA transfection reagent (Sigma). 48h and 72h later viral particle containing media was harvested ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cell disruption was carried out by vortexing (3 cycles, 5 min each) at 4°C using 0.2 ml of glass beads (425-600 μm; Sigma). For the Rpb3 immunopurification ...
-
bioRxiv - Cell Biology 2022Quote: ... was revealed with 5-bromo-4-chloro-3-indolyl β-D galactoside (X-gal) (Sigma-Aldrich, Carlsbad, CA, USA). Diploid cells were grown on SC medium supplemented with 20 mg/L adenine hemisulfate ...
-
bioRxiv - Microbiology 2024Quote: ... Coloring reactions were performed with nitro blue tetrazolium/5-Bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) solution (Sigma), and then sections were washed in PBS ...
-
bioRxiv - Molecular Biology 2024Quote: ... were incubated with 3 µg of D-Val-Leu-Lys 4-nitroanilide dihydrochloride chromogenic substrate (S-2251) (Sigma-Aldrich) in the presence of 1 µg of rFhENO or 1% BSA as negative control in a test volume of 100 µL ...
-
bioRxiv - Microbiology 2024Quote: ... Liver and spleen samples from experimentally infected fish for 3 days were fixed in 4% paraformaldehyde (PFA, Sigma, USA), embedded in paraffin and sectioned (4 μm ...
-
bioRxiv - Neuroscience 2024Quote: ... before being cut into 250μm parasagittal slices using a McIlwain tissue chopper and placed on Millicell membrane (3 to 4 slices each per animal, 0.4 μm membranes, Merck Millipore) in 50% BME (41010026 ...
-
bioRxiv - Neuroscience 2024Quote: ... The device was then pulsed with 3 bolus each of 4 different concentrations of dopamine hydrochloride (Sigma, H8502-10G), 0.5 μM ...
-
bioRxiv - Biochemistry 2024Quote: ... Colour was developed by adding 100 µL of 3 mg/mL disodium 4-nitrophenyl phosphate solution (DNPP, Sigma Aldrich) dissolved in 100 mM Tris ...
-
bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged CLAMP proteins were eluted for 3 h at 4°C on a rotating wheel in the presence of 0.5 mg/ml FLAG-Peptide (Sigma) in 1 ml Buffer C containing 1x PIC ...
-
bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged MSL proteins were eluted for 3 h at 4°C on a rotating wheel in the presence of 0.5 mg/ml FLAG-Peptide (Sigma) in 1 ml Elution buffer ...
-
bioRxiv - Biophysics 2023Quote: ... Peak fractions were concentrated to ∼ 3-4 mg mL-1 using a 100 kDa MWCO centrifugal concentrator (Millipore Sigma) for cryo-EM sample preparation.
-
bioRxiv - Cell Biology 2023Quote: The cell proliferation assay was determined by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay (Sigma). All cancer cell lines were seeded in 24-well plates (2×104 cells/well) ...
-
bioRxiv - Plant Biology 2023Quote: ... spy-3 and spy-4 were sprayed with either 50 µM or 100 µM BA (6-benzylaminoadenine, Sigma B3408) or mock [200 mM NaOH] three times ...
-
bioRxiv - Bioengineering 2023Quote: ... they were incubated with 4 mg/mL of N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC; Sigma-Aldrich, Schnelldorf, Germany) dissolved in 100 mM 2-(N-morpholino ...
-
bioRxiv - Microbiology 2023Quote: Infected S10-3 cells seeded onto coverslips were fixed in 4% PFA and permeabilized in 0.1% Trion-X100 (Sigma). For co-detection of RNA and capsid ...
-
Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... and then blocked for 3 hr at room temperature or overnight at 4 °C in BSA (Sigma-Aldrich A2153) 3% in PBS-T ...
-
bioRxiv - Bioengineering 2024Quote: ... the matrices were incubated with 4 mg/mL N-(3- dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC; Sigma‒Aldrich, Schnelldorf, Germany) dissolved in 0.1 M 2-(N-morpholino)ethanesulfonic acid (MES ...
-
bioRxiv - Cancer Biology 2024Quote: ... the cells were washed and followed by 3-hour incubation with 0.5 mg/mL MTT (3-(4, 5-Dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide) (Sigma-Aldrich). Cell apoptosis was measured with Annexin V Apoptosis Detection Kit I (BD Pharmingen ...
-
bioRxiv - Cancer Biology 2022Quote: ... 5’-GACAUAUUUGAUAAACUUAAA-3’, 5’-GUUAUCAGUCUGAGCCAGG-3’), human DRP1 (5’-GCAGAAGAAUGGGGUAAAU-3’, 5’-UCCGUGAUGAGUAUGCUUU-3’, 5’-GAGGUUAUUGAACGACUCA-3’) non-targeting scrambled siRNA (Sigma, SIC001-1NMOL) for 48-72 hours using Lipofectamine RNAiMAX (Invitrogen ...
-
bioRxiv - Developmental Biology 2021Quote: ... All live embryos were cultured in 10μl microdroplets of KSOM (potassium Simplex Optimized Medium; (Lawitts and Biggers, 1991)) with a mineral oil (Sigma, M8410) overlay inside an incubator (Thermo Fisher Scientific ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... pH 7.5 supplemented with 50 mM potassium sulphate (Honeywell, NC, USA) to a final concentration of 0.2 mg/mL protein and 5x SYPRO orange (Sigma-Aldrich). A temperature gradient of 25 to 70°C (heating rate 1°C per min ...
-
bioRxiv - Developmental Biology 2021Quote: ... The fertilized eggs were transferred to 5 μL droplets of potassium simplex optimization medium with amino acids (KSOMaa) containing 0.00025% polyvinyl alcohol (P8136-250G; Sigma-Aldrich) and 100 μM ethylenediaminetetraacetic acid on a glass-bottomed dish and placed in an incubation chamber (Tokai Hit ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were resuspended in sorbitol buffer (1.2 M sorbitol and 20 mM potassium phosphate buffer, pH 7.2) and spheroplated with β-mercaptoethanol (M7522; Sigma-Aldrich) and zymolase at 30°C ...
-
bioRxiv - Bioengineering 2022Quote: ... Heat inactivated fetal bovine serum (FBS), Tween20, Igepal CA-630, Trizma hydrochloride, and Potassium chloride solution (BioUltra, ∼1 M in H2O) were purchased from Sigma. Proteinase K was purchased from Qiagen ...
-
bioRxiv - Cancer Biology 2020Quote: ... in 50mM potassium phosphate was applied to all cells prior to treatment with or without 1 mM ascorbic acid (Sigma). Both catalase and ascorbic acid were prepared fresh for each experiment.
-
bioRxiv - Systems Biology 2022Quote: ... but with modified phosphate buffer proportions of potassium dihydrogen phosphate (6.309 g/L) and potassium hydrogen phosphate (9.34 g/L) at pH 6.8 and supplemented with various glucose concentrations (Sigma-Aldrich, US).
-
Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... each colony from pre-sporulation GNA medium was inoculated in 700 μL of liquid sporulation medium (0.1% potassium acetate (Sigma P1190), 0.005% zinc acetate (Sigma Z0625 ...
-
bioRxiv - Plant Biology 2022Quote: ... We removed the osmium solution and placed directly into 2.5% potassium ferrocyanide (#9387-100G; Sigma-Aldrich, St Louis, MO, USA) in 0.1 M sodium cacodylate at pH 7.4 for 90 minutes at room temperature ...
-
bioRxiv - Biochemistry 2024Quote: ... Potassium bicarbonate (KHCO3-) was added in the presence of 25 μg mL-1 carbonic anhydrase (CAII from bovine, Sigma, USA) to the pH 7.0 BTP buffered cells in the electrode chamber ...
-
bioRxiv - Bioengineering 2023Quote: ... The grid was subsequently washed three times in 0.05 M phosphate buffer (freshly prepared using dihydrogen potassium phosphate (Sigma–Aldrich) and dipotassium hydrogen phosphate (Merck) ...
-
bioRxiv - Biochemistry 2022Quote: ... 150 mM potassium acetate and 5 mM magnesium acetate) plus a cocktail of protease inhibitors (Sigma-Aldrich, St. Louis, MO). Cells were homogenized by 10 passages through a 22G needle ...
-
bioRxiv - Neuroscience 2024Quote: ... The tissue was rinsed in cacodylate buffer (recipe) and immersed two times in a mixture of 1% osmium and 0.8% potassium ferrocyanide (Sigma Chemical), each for 1.5 hours ...
-
bioRxiv - Microbiology 2024Quote: ... the larvae were stabbed in the central line and the hemolymph removed and placed in 1 mL of Insect Physiological Saline (IPS; 150mM Sodium Chloride, Fisher, S271; 5mM potassium chloride, Sigma, P-8041 ...
-
bioRxiv - Cancer Biology 2024Quote: ... #4 inguinal mammary glands were fixed in 4 % PFA overnight and then stained for 48 hours in carmine-alumn staining solution (2% w/v Carmine, Sigma; 5 % w/v Aluminium Potassium Sulfate, Sigma). After the desired colour had developed ...
-
bioRxiv - Genetics 2021Quote: ... 3 mM MgCl2 (Sigma, 7786-30-3), 0.1% Tween-20 (Sigma ...
-
bioRxiv - Microbiology 2022Quote: ... and EIF4E2 shRNA#3 (sh4EHP#3) (Sigma,). GIGYF2 shRNA#1 (shGIGYF2#1 ...
-
bioRxiv - Cancer Biology 2022Quote: ... covered with 3-aminopropyltrimethoxysilane (3-APTS, Sigma) for 3 min for activation ...
-
bioRxiv - Neuroscience 2021Quote: ... oxygenated DAB (3-3’-diaminobenzidine, Sigma-Aldrich) was dissolved in 0.1 N HCl at a concentration of 5.4 mg/ml and subsequently diluted ten-fold into sodium cacodylate buffer (pH 7.4 ...
-
bioRxiv - Bioengineering 2022Quote: ... 3-mercaptobenzoic acid (3-MBA, Sigma-Aldrich)-capped gold nanoclusters (3-MBA-Au nanoclusters ...
-
bioRxiv - Biochemistry 2024Quote: ... 3% SDS (Sigma-Aldrich, 151-21-3), 25 mM β-glycerophosphate (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2024Quote: ... 5 mM 3-methyladenine (3-MA, Sigma) or water (vehicle)46 ...
-
bioRxiv - Cancer Biology 2024Quote: ... ultracel-3 (>3 kDa cutoff) concentrators (Millipore). 30 µl of concentrate was loaded for western blot analysis.
-
bioRxiv - Immunology 2020Quote: ... and S100A9 (rA9) were concentrated using an Amicon Ultra-4 centrifugal filter device with a 3 kDa molecular-weight cutoff (Millipore). The primary sequence ...
-
bioRxiv - Cell Biology 2020Quote: ... and Ypt1-7xHis were mixed in a 1:3 molar ratio and incubated overnight at 4°C with calf intestinal alkaline phosphatase (Sigma) to hydrolyze nucleotide ...
-
bioRxiv - Developmental Biology 2020Quote: ... and then incubated with nitro blue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) substrates (Sigma-Aldrich) in NTM (100mM NaCl ...
-
bioRxiv - Biochemistry 2020Quote: ... and the blotting signals were chemically visualized with either the nitro-blue tetrazolium/5-bromo-4-chloro-3′-indolyphosphate (NBT/BCIP) chromogenic assay (Sigma) or infrared scanner ...
-
bioRxiv - Neuroscience 2021Quote: ... the NMDA glutamate receptor antagonist [3H]3-(2-carboxypiperazin-4-yl) propyl-1-phosphonic acid (CPP, 10 μM, Sigma-Aldrich) and a selective group III metabotropic glutamate receptor agonist ...