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Citations for Millipore Sigma :
851 - 900 of 10000+ citations for Potassium 3 4 difluorophenyl trifluoroborate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and activating transcription factor 3 (ATF-3, HPA001562, Millipore). After three washes with PBS ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mg/ml 3′,3′-diaminobenzidine tetrahydrochloride hydrate (Sigma), 24 units/ml catalase from bovine liver (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 mM 3-methoxybenzylamine (3-MBZ, Sigma-Aldrich #159891), and 0.5 mM benzylamine (BZA ...
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bioRxiv - Cell Biology 2020Quote: ... then rinsed 3 times in PBS (5 minutes each) and embedded in 4% low gelling temperature agarose in PBS (Sigma-Aldrich A0701). 55 μm slices of the ovaries were cut with a vibratome (Leica VT 1000 S ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μg of pSC2G-CFTR23 plasmid was co-transfected with 1 μM single-stranded DNA repair template (synthesized by Sigma, Table 3) comprising the CFTR-W1282X and silent protospacer adjacent motif (PAM ...
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bioRxiv - Developmental Biology 2020Quote: ... Wild-type ICR females (3-4 weeks old) were super-ovulated by sub-cutaneous injection of pregnant mare’s serum gonadotropin (PMSG) (Sigma-Aldrich, Rehovot, Israel) (5 units ...
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bioRxiv - Microbiology 2020Quote: ... primary antibodies were mouse-anti-ROP2/3/4 (1:250 dilution, 4A7, (58)) and rat anti-HA (1:500, clone 3F10, Sigma Aldrich, 11867431001), and secondary antibodies were goat anti-mouse IgG-Alexa 647 (1:2000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed at 4°C for 3 h using 5 µg of an anti-FLAG M2 antibody (Sigma-Aldrich, F1804, mouse). For RIP-Seq ...
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bioRxiv - Molecular Biology 2020Quote: ... The bound probe was visualized using 5-bromo-4-chloro-3 indoyl phosphate (NBT/BCIP; Sigma-Aldrich Corp., St. Louis, MO, USA). Bright field images were captured on the A1+ Nikon confocal microscope fitted with a DS-Ri1-U3 color camera at 20x magnification and 4076×3116 pixel resolution ...
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bioRxiv - Cell Biology 2020Quote: ... The liver tissue was cut into 3-4 mm pieces and incubated with 1 mM EGTA (Sigma-Aldrich, Cat. no. E0396-10G) in 1 x DBPS (Gibco™ ...
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bioRxiv - Biochemistry 2022Quote: ... pLANT-2/RIL–RFC[1+5] was co-transformed with pET(11a)-RFC[2+3+4] into BLR(DE3) cells (Novagen, Madison, Wisconsin). The cell lysate was clarified by centrifugation ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from tendon-like constructs (n=4 animals, 3 constructs each) using TRI® Reagent (Sigma-Aldrich; Vienna, Austria) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Patch pipettes with a tip resistance of 3 to 4 MΩ were filled with (in mM): K-gluconate (135 mM, Sigma; G4500-100G), EGTA (0.2 mM ...
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bioRxiv - Immunology 2022Quote: Fabs (2-4 mg/ml) were mixed with a 2 to 3-fold molar excess of fen-G4 or fentanyl (Sigma F-013) in Dulbecco’s PBS and incubated for 1-2 hours at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Surgical specimens were washed twice in phosphate buffered saline (PBS) and fixed for approximately 3 hours in 4% paraformaldehyde (Sigma, St. Louis). Tissue was washed two times in PBS and incubated at 4°C for 3-5 days in 30% sucrose ...
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bioRxiv - Neuroscience 2020Quote: A stock solution (74.8 mM) of the MEK inhibitor 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one (PD98059, PD hereafter, Sigma-Aldrich, Argentina) was conserved at −20 °C and freshly diluted in DMSO to the desired concentration (11.22 mM ...
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bioRxiv - Biochemistry 2020Quote: ... were pooled together (6 mL) and concentrated/buffer exchanged using an Amicon® Ultra-4 Centrifugal Filter Units (3 kDa, Millipore, Ireland). The final volume of the purified bacteriocin protein fraction was reduced to 200 μL ...
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bioRxiv - Plant Biology 2020Quote: ... Detection was performed by adding 200 μl of western blue substrate (SIGMAFAST™ BCIP®/NBT = 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium, Sigma) followed by incubation at room temperature in a dark and moist environment until the reagent’s color became visible ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated for 3 days at 37°C before overlay medium was removed and cells were fixed with 4% PFA (Sigma-Aldrich, MA) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... COMMD2 immunoblots were blocked for 1 hr at room temperature in 3% milk TBST and probed overnight at 4°C with the primary antibody (Sigma ref# HPA044190) suspended in 3% milk TBST ...
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bioRxiv - Molecular Biology 2024Quote: ... The number of viable cells was determined by mitochondrial conversion of 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma Aldrich, Missouri, USA) to formazan ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by two additional PBS-T washes and then blocking - for 3 h at room temperature or overnight at 4°C - in BSA (Bovine Serum Albumin, Sigma-Aldrich #A2153) 3% in PBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... Fixed cells were incubated with staining solution (1mg/mL of X-Gal,5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside; Sigma Aldrich #11680293001)(Lorenzo ...
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bioRxiv - Cell Biology 2023Quote: ... For NMR-experiments the proteins were concentrated by repeated ultrafiltration (Amicon Ultra-4 Ultracel-3 kDa centrifugal filter device, Merck Millipore, Burlington, USA).
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bioRxiv - Immunology 2024Quote: ... Peritoneal macrophages (PerMacs) were collected from the peritoneal cavity via gavage 4 days after intraperitoneal injection of 3% brewer thioglycolate medium (Sigma-Aldrich, B2551) and plated in in medium with 10% FBS ...
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bioRxiv - Genomics 2024Quote: ... Samples were stained with 1xTBS with 0.1% (v/v) Tween-20 and 3 mg/mL PVSA with 2ug/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, D9542) for 5-10 minutes at room temperature manually and rinsed three times with 1xTBS with 0.1% (v/v ...
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bioRxiv - Genomics 2024Quote: ... formamide in 1xTBS with 0.1% (v/v) Tween-20 and 3 mg/mL PVSA with 2ug/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, D9542). Readout probes ...
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bioRxiv - Cancer Biology 2024Quote: ... Buffer on top of cells was collected and further incubated with 3 mM 4-nitrophenyl-N-acetyl-β-D- glucosaminide substrate (Sigma, N9376) for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then blocked for a minimum of overnight and up to three days at 4°C in a blocking buffer of filtered 3% bovine serum albumin (Sigma A9418- 50G), 0.02% Tween-20 (Thermo 28320) ...
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bioRxiv - Biochemistry 2022Quote: ... Cytidine 3′, 5′ cyclic monophosphate (3′, 5′ cCMP) and Cytidine 2′, 3′ cyclic monophosphate (2′, 3′ cCMP) were purchased from Sigma-Aldrich (Bangalore, India) and used without further purification ...
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bioRxiv - Molecular Biology 2024Quote: ... 5’-GACCAGAGACG TGTAGCAATG-3’, Reverse: 5’-ACAATGCTTTGACGATGCCATA-3’) and ACTB (Forward: 5’-CTGGAACGGT GAAGGTGACA-3’, Reverse: 5’-AAGGGACTTCCTGTAACAATGCA-3’) were ordered from Sigma Aldrich (Missouri, USA). cDNA synthesis was carried out using the QuantiTect Reverse Transcriptase Kit (Qiagen ...
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bioRxiv - Developmental Biology 2024Quote: 4-Hydroxytamoxifen (4-OHT) Treatments and Bromodeoxyuridine Incorporation: 4-OHT (T176, Sigma) treatment was performed as previously described (Mosimann et al. ...
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ER exit sites in Drosophila display abundant ER-Golgi vesicles and pearled tubes but no megacarriersbioRxiv - Cell Biology 2021Quote: ... the carcasses were transferred to 0.1 M PB and post-fixed in a mixture of 1% osmic acid (Tedpellco, cat # 018456) and potassium ferrocyanide (1.5% w/v, Sigma, cat # 60299-100G-F) at RT avoiding light ...
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bioRxiv - Physiology 2021Quote: Muscle fibers were impaled with 2 sharp microelectrodes filled with 2 M potassium acetate solution containing 1 mM sulforhodamine 101 (Sigma-Aldrich, Catalogue #S7635) to allow for visualization ...
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bioRxiv - Neuroscience 2022Quote: ... The silanized ion-sensitive barrel tip (5% trimethyl-1-chlorosilane in dichloromethane)was filled with a potassium ionophore I cocktail A (60031 Fluka distributed by Sigma-Aldrich, Lyon, France) and backfilled with 100 mM KCl ...
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bioRxiv - Plant Biology 2024Quote: ... The sample was then washed five times for 10 min each with SP buffer and transferred to a freshly prepared solution containing 1.5% potassium ferrocyanide (w/v, SIGMA Life Science, Lot# BCBV7953) and 2% OsO4 (v/v ...
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bioRxiv - Developmental Biology 2020Quote: ... and 4×10-4 M monothioglycerol (Sigma)) with BMP4 (1 ng/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... 4-nitroquinoline 1-oxide (4-NQO) (Sigma). Treatment conditions are indicated in the corresponding figures ...
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bioRxiv - Genomics 2020Quote: ... and 4 × 10−4 M monothioglycerol (Sigma). Media and cytokines were changed daily as follows (Gadue et al. ...
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bioRxiv - Neuroscience 2021Quote: ... 4-Aminopyridine (4-AP, Sigma, Cat# A78403), DL-AP5 (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... 4-hydroxytamoxifen (4-OHT) (Sigma-Aldrich; #H6278) was added to the retinal explant media after 7 DIV at 5 mM final concentration ...
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bioRxiv - Cancer Biology 2021Quote: ... 4-Hydroxytamoxifen (4-OHT, Sigma-Aldrich, USA). Further information on cell lines and clones in Supplementary Data.
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bioRxiv - Neuroscience 2021Quote: 4-aminopyridine (4-AP; Sigma Aldrich, USA) was used to block IKdr ...
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bioRxiv - Neuroscience 2021Quote: ... 4-Hydroxytamoxifen (4-OHT) (H6278, Sigma-Aldrich) was handled as previously described37 ...
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bioRxiv - Developmental Biology 2020Quote: ... and 4-hydroxytamoxifen (4-OHT, Sigma H6278) were mixed with 100% ethanol until completely dissolved ...
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bioRxiv - Developmental Biology 2021Quote: ... and 4×10-4 M monothioglycerol (Sigma)) with BMP4 (1 ng/ml ...
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bioRxiv - Microbiology 2021Quote: ... 1 µM 4-hydroxytamoxifen (4-OHT; Sigma) for 1h at 37°C prior to infection and during the course of infection ...
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bioRxiv - Cell Biology 2021Quote: ... 4-Hydroxytamoxifen (4-OHT, H7904, Sigma-Aldrich) was dissolved in DMSO as a concentrated solution of 10 mM and kept as single-use aliquots at –80 °C ...
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bioRxiv - Cell Biology 2024Quote: ... 4-hydroxytamoxifen (4-OHT) (H7904, Sigma-Aldrich) was added to cells at 1 μM working concentration for the indicated time.
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bioRxiv - Neuroscience 2023Quote: ... 2 mM 4-aminopyridine (4-AP; Sigma) was added to the perfusate ...