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Citations for Millipore Sigma :
601 - 650 of 10000+ citations for IL 13 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... Fc-PRL-13-c6 was grown in 200mL of BMGY media supplemented 1tab/50mL of cOmplete Protease Inhibitor Cocktail (Sigma; 11836145001) and 10uM Pepstatin A (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... THP-1 monocytes were differentiated to macrophages with medium containing 50 ng/mL phorbol 12-myristate 13-acetate (PMA) (Sigma #P1585) for 3 days ...
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bioRxiv - Immunology 2023Quote: ... and 10-12 were treated with 100 µL of embryo water plus 1 µg/mL H2DCFDA and 400 ng/ml phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich). The plates are covered with aluminum foil and incubated at 28°C for 2.5 hours ...
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bioRxiv - Neuroscience 2024Quote: ... underwent anesthesia through the intraperitoneal injection of a combination of 65 mg/kg ketamine (Daiichi-Sankyo) and 13 mg/kg xylazine (Sigma-Aldrich). For the CG/SMG (Fig ...
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bioRxiv - Neuroscience 2024Quote: ... Otr-Cre male mice (aged 5–7 weeks) were anesthetized using a combination of 65 mg/kg ketamine (Daiichi-Sankyo) and 13 mg/kg xylazine (Sigma-Aldrich) via intraperitoneal injection ...
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bioRxiv - Neuroscience 2024Quote: ... underwent anesthesia through the intraperitoneal injection of a combination of 65 mg/kg ketamine (Daiichi-Sankyo) and 13 mg/kg xylazine (Sigma-Aldrich). An incision around the thoracic region of the SC was made ...
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bioRxiv - Molecular Biology 2024Quote: ... THP-1 monocyte differentiation was initiated by incubating the cells with 10 ng/mL phorbol 12-myristate 13-acetate (PMA; Sigma Aldrich) for 2 days at 37°C in a 5% CO2 environment ...
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bioRxiv - Biochemistry 2024Quote: ... The sample was divided into two parts (supernatant and pellet) that were TCA precipitated and analyzed by 13% SDS PAGE stained by Sypro Orange (Sigma-Aldrich). Experiments were performed at least twice (n ≥ 2).
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... Host cell lines included U937 monocytes (ATCC CRL-1593.2) which were differentiated into macrophages with 12-O-tetradecanoylphorbol-13-acetate (TPA, Sigma-Aldrich P1585) and A ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... cells were plated on 24-well plates at 3x105 cells/well with 0.1 μg/mL 12-O-tetradecanoylphorbol-13-acetate (TPA, Sigma-Aldrich P1585) to promote differentiation ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction was started after 5 min preincubation by adding 13 µL of a microsome mix from the liver of Sprague-Dawley rats (Sigma Aldrich, 20 mg protein/mL in 0.1 M phosphate buffer ...
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bioRxiv - Cell Biology 2024Quote: ... 106 THP-1 cells were transferred to each of the six-well plates and cultured with 100ng/mL phorbol 12-myristate 13-acetate (PMA) (P8139, Sigma-Aldrich) for 24h ...
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bioRxiv - Cell Biology 2024Quote: ... animals in the D-GAL groups received during 13 weeks daily intraperitoneal (IP) injections of 600 mg/kg of D-galactose (D-gal, G53688, Sigma Aldrich) resuspended in phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2024Quote: ... THP-1 cells were plated to 24-well plate at a concentration of 2 × 105 viable cells per well and incubated for 48 h in 1 ml/well of media supplemented with 50 nM phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich) for differentiation into macrophages ...
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bioRxiv - Immunology 2021Quote: ... the amplified IL-22 gene was cloned into a pET32a (+) vector (Novagen, USA) using two restriction sites as shown in Figure 1b ...
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bioRxiv - Neuroscience 2019Quote: ... interleukin alpha (IL-1a) (3 ng/ml; Sigma-Aldrich; Cat. No. I3901-5UG) and complement component 1 ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-acetylated α-tubulin (clone 6-11B-1, Sigma-Aldrich, St. Louis, IL), anti-β-tubulin (E7 ...
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BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?bioRxiv - Immunology 2019Quote: ... Stimulated cells were treated with 68.7 ng/mL (8000IU) IL-4 (Sigma-Aldrich) and 5 μg/mL anti-CD40 (BioXCell ...
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bioRxiv - Immunology 2020Quote: ... IL-2 and methanol were all purchased from Sigma-Aldrich (St. Louis, MO). Poly(lactide-co-glycolide)-block-poly(ethylene glycol)-succinimidyl ester (PLGA-PEG-NHS ...
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bioRxiv - Biochemistry 2021Quote: ... NTPs were from Chem-Impex International (Wood Dale, IL) and all other chemical reagents were from Sigma-Aldrich. The pH of Tris-HCl was adjusted at 23 °C.
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bioRxiv - Bioengineering 2022Quote: ... in medium containing 50 ng/mL IL-6 (Peprotech),10 ng/mL IL-15 and 15 ng/mL interferon-α2B (Sigma-Aldrich).
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bioRxiv - Immunology 2022Quote: ... IL-10 -/- mice were given streptomycin (5 g/L, Cat#S9137, Sigma-Aldrich) in the drinking water ad libitum for 24 hr ...
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bioRxiv - Molecular Biology 2023Quote: ... HPAECs were treated with 1 ng/ml IL-1β (Millipore Sigma, Catalog# H6291) for 8 hours.
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bioRxiv - Microbiology 2023Quote: ... Cytokines were detected from vitreous humor using commercial kits IL-1β (Sigma-Aldrich), TNFα (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... Anti-mouse agarose or mouse agarose (both Sigma) were added for 1 hour at 40C prior to 3 washes in Lysis Buffer ...
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bioRxiv - Neuroscience 2019Quote: ... Mouse anti-mouse NeuN (Millipore, MAB377, 1:100), and Rabbit Anti-MBP (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... and Mouse-anti-mouse/rat-NeuN (1;200, Millipore). Secondary antibodies include Goat-anti-Chicken-IgG-488 (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... cells were grown to a density of 8.0 × 105 cells/mL and treated with 25ng/mL Phorbol 12-Myristate 13-Acetate (PMA) (Sigma-Aldrich, Catalogue #P8139) for 4 days ...
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bioRxiv - Molecular Biology 2021Quote: ... ACC 57), K562 (human, DSMZ Germany ACC 10), and Molm-13 (human, DSMZ Germany ACC 554) were cultured in RPMI1640 (Sigma-Aldrich R8758) supplemented with 10 % heat-inactivated FCS and 1 % penicillin streptomycin mixture ...
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bioRxiv - Neuroscience 2020Quote: Enucleations were performed on 13 dpf wildtype and tmt-opsin1b mutant juvenile fish upon anaesthetization with 0.03% tricaine (MS-222) (Sigma, St. Louis, MO). After the surgery ...
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bioRxiv - Neuroscience 2021Quote: ... Baseline fluorescence was recorded for 30s before application of either vehicle or 1μM phorbol 12-myristate 13-acetate (PMA) (Sigma Aldrich, Cat# P8139) and imaged every 10s for 5 minutes at 50% laser power ...
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bioRxiv - Systems Biology 2020Quote: ... For macrophage differentiation U937 cells were suspended in medium with 20 ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma Aldrich, #P1585) and plated in 96-well microplates with µClear®flat bottom (Greiner ...
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bioRxiv - Plant Biology 2021Quote: ... The dissolved samples were filtered through 13 mm diameter Millex filters with 0.22 μm pore size nylon membrane (Millipore, Bedford, MA, USA) and placed into opaque microcentrifuge tubes.
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bioRxiv - Cancer Biology 2020Quote: HL60 cells were plated at a density of 4×105 cells/ml and differentiated with 12-O-tetradecanoylphorbol-13-acetate (TPA) (Sigma, Darmstadt, Germany) at a final concentration of 32 nM for 6 days ...
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bioRxiv - Microbiology 2022Quote: ... and macrophage-like differentiation was induced upon a twenty-four hours treatment with 100ng/ml of phorbol 12-myristate 13-acetate (PMA) (Sigma cat. P1585). Calu-3 cells media was instead supplemented with a 1x final concentration of non-essential amino-acids (Sigma cat ...
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bioRxiv - Plant Biology 2020Quote: ... The dissolved samples were filtered through 13-mm diameter Millex filters with a 0.22-μm pore size nylon membrane (Millipore, Bedford, MA, USA). Filtered extract (10 μl ...
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bioRxiv - Cell Biology 2020Quote: A MilliPlex MILLIPLEX® MAP Human Cytokine/Chemokine/Growth Factor Panel A-Immunology Multiplex Assay (HCYTA-60K-13, Millipore Sigma, Burlington, Massachusetts) was run on a Luminex 200 Instrument (LX200-XPON-RUO ...
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bioRxiv - Immunology 2020Quote: ... cell suspensions in RPMI medium supplemented with 10% FBS were re-stimulated for 4 hr with phorbol 12-myristate 13-acetate (PMA, Sigma, 50ng/mL) and ionomycin (Sigma ...
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bioRxiv - Genetics 2019Quote: ... Tissues were mounted between slide and coverslip (24 mm × 24 mm) with an imaging spacer (1 well, diameter × thickness: 13 mm × 0.12 mm Grace Bio-Labs SecureSeal imaging spacer, Sigma-Aldrich) using mounting medium (ibidi) ...
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bioRxiv - Microbiology 2022Quote: wtKSHV inoculum was obtained from BCBL-1 cells (5×105 cells/ml) induced with 20 ng of TPA (12-O-tetradecanoylphorbol-13-acetate; Sigma, St.Louis, USA)/ml ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were seeded at a density of 1x105 cells/ml in 96 well plates and differentiated for 48 hours with 25 nm PMA (phorbol-12-myristate-13-acetate, Sigma-Aldrich, #P1585). Cells were then left to rest for 24 hours in fresh media containing no PMA prior to bleomycin exposures.
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated on 6-well cell culture-treated plates and differentiated using 100 nM phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, P1585) for 72 h in serum-free RPMI 1640 medium (SFM ...
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bioRxiv - Molecular Biology 2022Quote: ... Stimulation of Jurkat cells for 2-7 hour experiments was achieved with 50ng/ml Phorbol 12-myristate 13-acetate (PMA, Sigma-Alrich, P8139) and 500ng/ml ionomycin calcium salt from Streptomyces conglobatus (ionomycin ...
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bioRxiv - Neuroscience 2022Quote: ... animals were first pre-incubated for 10 minutes in low Mg2+ HL3 (10 mM Mg2+, 0 mM Ca2+) containing 2 µM of phorbol ester Phorbol-12-myristat-13-acetat (PMA (Sigma Aldrich, Germany), stored at −20°C as 10 mM stock in DMSO ...
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bioRxiv - Neuroscience 2022Quote: ... animals were pre-incubated for 10 minutes in HL3 (0 mM Ca2+) containing 2 µM of phorbol ester Phorbol-12-myristat-13-acetat (PMA (Sigma Aldrich, Germany), stored at −20°C as 10 mM stock in dimethyl sulfoxide (DMSO)) ...
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bioRxiv - Immunology 2023Quote: ... the cells were incubated in complete RPMI media containing 200 ng/mL of Phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, #P8139) for 48 hours ...
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bioRxiv - Microbiology 2023Quote: ... Jurkat Timer clones were treated for 24 h with a combination of T-cell stimulants: phorbol 12-myristate 13-acetate (SIGMA, Cat# P1585) at a final concentration of 20 ng/ml and ionomycin (Nacalai Tesque ...
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bioRxiv - Developmental Biology 2023Quote: ... were dissected from distal regions of the lung and placed on Nucleopore Track-Etched Membrane disks (13 mm, 8 µm pore, poly- carbonate) (Sigma, Cat#WHA110414) floating on top of 500 µl of human lung ALI explant media (Advanced DMEM/F-12 (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... TC-HSMAM1 and Cas9-TC-HSMAM1 cells were maintained in a complete RPMI-1640 medium and 100 ng/mL of phorbol 12-myristate-13-acetate (PMA) (Sigma-Aldrich, USA) at 37°C for 48 h to induce cell differentiation to macrophages 31 ...
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bioRxiv - Plant Biology 2023Quote: ... Separate 50 µl (same aliquot from the total 300 µl) were separated on 13% SDS-PAGE gel for immunoblot using anti-Histone 3 (Sigma #06-755) at 1:3000 dilution with 1:10000 anti- rabbit secondary antibody ...