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Citations for Millipore Sigma :
551 - 600 of 10000+ citations for IL 13 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Intracellular staining was performed after stimulation of single-cell suspensions with Phorbol 12-myristate 13-acetate (PMA at 50 ng mL−1, Sigma), ionomycin (0.5 μg mL−1 ...
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bioRxiv - Immunology 2024Quote: Whole intact tumours were harvested on days 10-13 post engraftment and immediately immersed in fixative solution (1% PFA, 75 mM L-lysine [Sigma, Cat ...
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bioRxiv - Immunology 2024Quote: CNS and splenic cell suspensions were stimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich, #P8139), 1 µM Ionomycin (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... THP-1 were seeded into a 6 well plate at 2 x 105 cells/well and treated with 20 ng/mL phorbol-12-myristate 13-acetate (PMA, Sigma) in RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... anti-H2A acidic patch (07-146), anti-H2B (07-371) and anti-H4 (05-858, clone 62-141-13) purchased from Millipore. Anti-H2A (Novus NBP1-58095) ...
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bioRxiv - Microbiology 2023Quote: THP-1 cells were seeded at 5 ×105/mL in 24 well plate and differentiated to macrophage-like cells by treatment with 200 nM Phorbol 12-myristate 13-acetate (PMA; Sigma) for 18 hrs ...
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bioRxiv - Immunology 2023Quote: ... Interphase cells were collected and incubated at 37°C in 5% CO2 for 6 hours with 20ng/ml Phorbol 12-Myristate 13-Acetate (PMA, Sigma), 1µg/ml Ionomycon (Sigma ...
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bioRxiv - Immunology 2023Quote: ... total tissue homogenate/digest was directly re-stimulated with 50 ng/ml phorbol-13-myristate-12-acetate (PMA) (Sigma-Aldrich) and 0.5 μg/ml ionomycin (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2023Quote: ... THP-1 cells were stimulated with either 100ng/mL (for M1 macrophages) or 50ng/mL (for M2 macrophages) of phorbol 12-myristate 13-acetate (PMA, Sigma) for 48h ...
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bioRxiv - Genetics 2023Quote: ... by gel filtration chromatography using the Superdex 200 Increase 10/300 GL (Cytiva) and concentrated to 13 mg/mL using a 30 kDa cutoff MWCO concentrator (Millipore). Cryo-EM grids were prepared with an EM GP2 Automatic Plunge Freezer (Leica Microsystems) ...
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bioRxiv - Immunology 2023Quote: ... TIGIT-/- and TIGIT-/-ICOSL-/- NOD mice were incubated 4 hours at 37°C with Phorbol 12-myristate 13-acetate (PMA 10 ng/ml; Sigma), ionomycin (500 ng/ml ...
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bioRxiv - Microbiology 2023Quote: ... or fusion proteins were generated by transduction with LKO-derived lentiviral vectors (13) followed by selection in 1μg ml−1 puromycin (Sigma-Aldrich). HeLa cells stably expressing TRIM fusions were generated by transduction with LHCX-derived vectors followed by selection in 100μg ml−1 hygromycin B (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... Lipopolysaccharides from Escherichia coli 055: B5 (L2880) and Phorbol 12-myristate 13-acetate (PMA) (P1585) were obtained from Sigma-Aldrich. Yoda1 (5586 ...
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bioRxiv - Neuroscience 2023Quote: ... and the cells were plated at a density of 75,000 per well of 13 mm diameter onto glass coverslips previously coated with poly-L-lysine (10 µg/mL, Sigma). Cultures were maintained in neurobasal medium supplemented with B-27 ...
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bioRxiv - Immunology 2023Quote: ... of Escherichia coli O111:B4 or O127:B8, and Phorbol 12-myristate 13-acetate (PMA, CAS: 16561-29-8) were obtained from Sigma. Adenosine 5’-triphosphate disodium salt (ATP ...
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bioRxiv - Cell Biology 2024Quote: ... Michael Bassik at Stanford University) were differentiated into macrophage-like cells using 20ng/ml phorbol 12-myristate 13 acetate (PMA, Sigma) for 72 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... Cy3-mouse-anti-mouse aSMA (Sigma C6198), mouse anti-mouse αSMA (Sigma A2547) ...
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bioRxiv - Cell Biology 2020Quote: Macrophages were differentiated from THP1 cells using serum-free DMEM supplemented with 10 ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich). After 48h ...
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bioRxiv - Developmental Biology 2021Quote: SH-SY5Y cells at 60 – 70% confluency were induced to differentiate using 13-cis retinoic acid at a concentration of 2 µM (Sigma-Aldrich) in the growth media ...
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bioRxiv - Bioengineering 2019Quote: ... the THP-1 cells were cultured in the culture medium used above and supplemented with 150 nM phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) for 72 hours ...
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bioRxiv - Plant Biology 2019Quote: ... myc2 and T-DNA mutant seeds (Supplementary Table 13) were grown on agar plates containing LS medium supplemented with or without 50 µM MeJA (392707, Millipore Sigma) for 9 days ...
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bioRxiv - Immunology 2019Quote: ... cells were first cultured for 4 hours in the presence of 50 ng mL−1 phorbol 12-myristate 13-acetate (Sigma-Aldrich), 1 µM ionomycin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... and plated at density 75,000 cells per 13-mm-diameter coverslip (Assistent, Germany) coated with 1 mg/ml poly-DL-lysine (Sigma-Aldrich, P9011) and 2.5 μg/ml laminin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... cells were plated on 24-well plates at 3×105 cells/well with 0.1 µg/mL 12-O-tetradecanoylphorbol-13-acetate (TPA, Sigma-Aldrich #P1585) to promote differentiation ...
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bioRxiv - Immunology 2021Quote: ... cells were either left unstimulated or activated for 4 h with phorbol 12-myristate 13-acetate (50 ng/ml; Sigma-Aldrich) and ionomycin (500 ng/ml ...
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bioRxiv - Neuroscience 2020Quote: PMA and TTX: MAPK pathways were activated intracellularly via PKC with 1µM-1nM phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich, #P1585). PMA treatments were applied as a 2° treatment in place of bicuculine ...
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bioRxiv - Cell Biology 2020Quote: ... and 0.2% tetramethylethylenediamine (TEMED) and 170 uL was transferred to a silicone mold with inner diameter of 13 mm (Sigma-Aldrich, GBL664107) attached to a parafilm-covered glass slide ...
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bioRxiv - Biochemistry 2022Quote: ... U937 cells were differentiated to macrophages by 24 h incubation with 20 nM phorbol 12-myristate 13-acetate (PMA, Sigma, P8139) followed by 24 h incubation in RPMI medium ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then left overnight at 37°C before restimulation with 50 ng/mL phorbol 12-myristate 13-acetate (Sigma-Aldrich) and 500 ng/mL ionomycin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... and melan-le cells were cultured in RPMI+10% FBS + 200 nM Phorbol 12-myristate 13-acetate (TPA) (Sigma-Aldrich, P8139).
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bioRxiv - Microbiology 2020Quote: ... 24-well plastic plates were seeded with THP-1 cells at a density of 30 ⨯ 104 cells per well in complete medium after being treated with 30 ng/mL phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich). These 24-well plastic plates where then incubated for 48 hs to induce maturation mediated by PMA toward adherent macrophage-like cells ...
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bioRxiv - Microbiology 2021Quote: ... 2 x 106 cells/ml stimulated for 12 hours with 100 ng/ml phorbol 12-myristate 13-acetate (PMA) and 1 µg /ml ionomycin (both from Sigma Aldrich). After stimulation ...
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bioRxiv - Microbiology 2020Quote: ... cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum were differentiated with phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich) 100nM for 48h at a density of 2.105 cells/mL ...
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bioRxiv - Immunology 2020Quote: ... THP-1 cells were differentiated into resting macrophages by resuspending the cells in growth medium containing 5 or 50 ng/ml phorbol-12-myristate-13-acetate (PMA; Sigma-Aldrich) and cultured for indicated time periods ...
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bioRxiv - Physiology 2019Quote: ... Cells were then incubated with activator Phorbol ester 12-O-tetradecanoylphorbol-13-acetate (PMA; Sigma, Schnelldorf, Germany; 0.1 μM; 6 h) with or without 1 μM PKC inhibitors Calphostin C (Tocris) ...
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bioRxiv - Bioengineering 2020Quote: ... at 2000 rpm for 5 minutes in the aqueous continuous phase (100 mL) containing 1% w/v of poly(vinyl acetate-co-alcohol) (PVA; MW 13-23 kDa, 98% hydrolyzed; Sigma-Aldrich) as stabilizer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Selected test agents included Imeglimin (Poxel SA), GLP1, metformin, an imidazoline (13) phospholipase C (PLC) pathway activator (BL11282, Metabrain Research) and a PLC inhibitor (U73122, SIGMA U6756).
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Ion transport modulators differentially modulate inflammatory responses in THP-1 derived macrophagesbioRxiv - Immunology 2021Quote: ... the THP-1 cells were differentiated into macrophages by treatment with 200 nM phorbol-12-myristate-13-acetate (PMA) (catalog no. P8139; Sigma-Aldrich) for 3 days.
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bioRxiv - Immunology 2021Quote: ... Cells (2.5×105 cells/ml) were differentiated with 200 nM phorbol-12-myristate-13-acetate (PMA, Sigma-Aldrich, product no.P-8139) for 48 h ...
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bioRxiv - Microbiology 2019Quote: ... THP-1 were differentiated into adherent macrophages by incubating for 48 h in the presence of 100 nM 2-O-tetradecanoylphorbol 13-acetate (Millipore Sigma), and subsequently infected with the indicated viruses at an MOI of 5 TCID50/cell.
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bioRxiv - Immunology 2021Quote: ... THP-1 cells differentiation into mature macrophages-like state was induced through incubation with 10 ng/mL of phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) for 48 hours in a humidified atmosphere ...
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bioRxiv - Neuroscience 2021Quote: ... Thioflavine S (T1892), Anti-Digoxigenin-AP (Fab fragments, 11093274910), and Phorbol 12- myristate 13-acetate (PMA, p8139) were ordered from Sigma-Aldrich.
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bioRxiv - Immunology 2020Quote: ... 2mM glutamine and 100 U/mL penicillin and 50 µg/mL streptomycin and differentiated into macrophages after treatment with 50 ng/ml phorbol-12-myristate 13-acetate (PMA, Sigma Aldrich) for 48 hours (8,19).
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bioRxiv - Immunology 2022Quote: ... Macrophages were generated by differentiating THP-1 monocytes with 100 ng/ml phorbol 12-myristate 13-acetate (PMA) (Millipore Sigma, P8139) for 24 h ...
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bioRxiv - Bioengineering 2022Quote: ... The nanopatterned flexible films were then washed with an amine-terminated poly(N-isopropylacrylamide) (pNIPAM) solution (13 µM in H2O, Mn = 2500 Sigma-Aldrich) for 24 hours on a tabletop rocker (55 rpm ...
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bioRxiv - Immunology 2022Quote: ... T cells were stimulated with 50 ng/ml phorbol 12-myristate 13-acetate (PMA) and 500 ng/ml ionomycin (Sigma-Aldrich) in the presence of GolgiStop (BD ...
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bioRxiv - Immunology 2022Quote: ... THP-1 monocytes are differentiated into macrophages by 36 h incubation with 20 nM phorbol 12-myristate 13-acetate (PMA, Sigma, P8139). The murine macrophage Raw 274.7 cells (ATCC ...
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bioRxiv - Molecular Biology 2022Quote: ... human monocytic cell-lines into macrophages was performed by treatment with 30 nM phorbol-12-myristate-13-acetate (PMA; Sigma-Aldrich) for up to 24 hours ...
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bioRxiv - Microbiology 2023Quote: U937-DC-SIGN cells were treated for 12 days with 160 nM Phorbol12-myristate-13-acetate (PMA) (Sigma-Aldrich®: P1585) and 30 ng/mL macrophage-colony stimulating factor (M-CSF ...
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bioRxiv - Immunology 2022Quote: ... cells were stimulated with phorbol 12-myristate 13-acetate (PMA, 25 ng/mL) and ionomycin (750 ng/mL; both Sigma-Aldrich) in the presence of GolgiStop (4 μl/6mL ...