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Citations for Millipore Sigma :
601 - 650 of 2153 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were blocked at room temperature for 1 h in 1% BSA (Sigma). Subsequently ...
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bioRxiv - Microbiology 2021Quote: ... for 1 h at 37 °C and alkylated with 20 mM IAA(Sigma) for 45 min at room temperature in darkness ...
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bioRxiv - Neuroscience 2020Quote: ... were incubated for 1 h at room temperature before mounting with Fluoromount (Sigma).
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bioRxiv - Immunology 2020Quote: ... The biopsies were subsequently digested with 1 mg/ml collagenase type H (Sigma) in 2% FCS in PBS at 37°C for 35 min under constant rotation ...
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bioRxiv - Physiology 2021Quote: ... the cells were starved for 16 h in glucose-free DMEM (Sigma–Aldrich), supplemented with 2 mM glucose ...
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bioRxiv - Microbiology 2020Quote: ... 1 h post-infection the indicated amounts of thapsigargin (Sigma-Aldrich®, 586006), nucleozin (Calbiochem® ...
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bioRxiv - Microbiology 2020Quote: ... cruzi epimastigotes were synchronized for 24 h with 20 mM of hydroxyurea (Sigma) [27] ...
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bioRxiv - Cell Biology 2021Quote: ... Blots were incubated with HRP-conjugated secondary antibodies (1 h room temperature; Sigma) and visualized by ECL (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... we cultured cells for 6 h with either PMA (100 ng/ml, Sigma), Ionomycin (1 µM ...
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bioRxiv - Neuroscience 2022Quote: ... immunofluorescence or western blot experiments: thiorphan (500 nM, 1 h; Sigma-Aldrich, Sweden), TTX (1 μM ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were incubated with adding DAPI/Hoechst 33258 (bis-Benzimide H, Sigma-Aldrich) diluted 1:10.000 in PBS for 10 minutes at room temperature ...
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bioRxiv - Pathology 2021Quote: ... mouse livers were perfused and digested with collagenase (Collagenase H, Sigma Chemical Company) as described previously 21 ...
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bioRxiv - Biophysics 2021Quote: ... #1.5 Plano GmbH) were cleaned by 1 h sonication in chloroform (472476, Sigma), followed by 1 h sonication in NaOH (6771 ...
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bioRxiv - Microbiology 2021Quote: ... and blocked during 1 h with 3 % BSA (Probumin) or 1% gelatin (Sigma) in PBS ...
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bioRxiv - Biophysics 2021Quote: ... and 0.75 mg/ml H-3506 hyaluronidase (Sigma-Aldrich, St. Louis, MO, USA) at 37 °C in a water bath with agitation for 60 min ...
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bioRxiv - Cell Biology 2022Quote: ... for 8 h with protein A/G agarose beads (Sigma-Aldrich, Burlington, Massachusetts) at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were fixed 24 h post-transfection with 2% (w/v) paraformaldehyde (Sigma) for 30 min ...
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bioRxiv - Immunology 2020Quote: ... cells were pre-incubated for 4 h with PMA 50 ng/ml (Sigma), ionomycin 1 µg/ml (Sigma ...
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bioRxiv - Immunology 2020Quote: ... for 1 h at RT in microscopy buffer (5% w/V BSA (Sigma), 0,06% w/V saponin (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... for 12 h before infection and LPS (100 ng/mL, Sigma-Aldrich, India) for 2 h before infection ...
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bioRxiv - Cell Biology 2020Quote: ... and Rabbit anti-guinea pig IgG (H+L) HRP (Sigma-Aldrich A5545, RRID:AB_258247). All were used at the 1:2000 dilution.
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bioRxiv - Biochemistry 2021Quote: ... cells were treated for 24 h with 2.5 µg/mL of U18666A (Sigma) following the transfection step.
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bioRxiv - Biochemistry 2021Quote: ... pre-incubated for 3-7 h with rabbit anti-FLAG antibody (Sigma-Aldrich) at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were reduced and alkylated for 1 h with 15 mM TCEP (Sigma) and 30 mM CAA (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... the vehicle was 10% DMSO/10% 2-hydroxypropyl-β-cyclodextrin (Sigma, H-107) in PBS ...
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bioRxiv - Microbiology 2020Quote: ... were incubated for 2 h at room temperature with anti-GFP (Sigma-Aldrich), mouse monoclonal anti-FLAG (M2 ...
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bioRxiv - Genetics 2020Quote: ... Heparin (Cat# H-3149) and SU5402 (Cat# SML0443-25MG) were from Sigma-Aldrich, CHIR99021 (Cat# 4423 ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were treated for 24 h with 5 μg/ml Doxycyclin (Sigma, D9891) for induced expression of the Cre-recombinase from the Dox-inducible locus of A2lox WT cells to excise the loxP-flanked mT expression cassette and bring the mG expression cassette under the transcriptional control of the Rosa26 gene locus ...
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bioRxiv - Bioengineering 2022Quote: After 24 or 48 h cells were fixed using 4% paraformaldehyde (Sigma–Aldrich) for 2 h followed by washing with PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... were incubated 1 h at 37 °C in Duolink blocking buffer (Sigma-Aldrich) and then for 1 h at room temperature with a mix of the two primary antibodies directed against the target proteins (anti-biotin to detect new H3.3-SNAP-biotin and anti-gH2A.X to detect sites of DNA damage ...
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bioRxiv - Cell Biology 2022Quote: ... and bisbenzimide H 33258 (1:1000, B1155; Sigma-Aldrich-Aldrich, St. Louis, MO) diluted in 3% BSA in PBS for 1 hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Samples were blocked for 1 h in 3% bovine serum albumin (Sigma-Aldrich) and subsequently incubated in primary antibodies (overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies included: goat anti-mouse IgG antibody (Merck Millipore AP308P, (H+L) HRP conjugate ...
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bioRxiv - Plant Biology 2022Quote: ... and incubated overnight with Helix pomatia Type H-1 sulfatase (S9626, Sigma-Aldrich) prepared as described by Graser et al (Graser et al. ...
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bioRxiv - Physiology 2023Quote: ... and stained with hematoxylin and eosin (H&E) or Masson’s trichrome (Sigma-Aldrich). To prepare frozen sections ...
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bioRxiv - Neuroscience 2022Quote: ... anti-rabbit IgG (H + L) (1/1,000, CF-568 Sigma-Aldrich, No. SAB4600084). The signal of the PHP2 huntingtin staining was amplified using Biotinyl-Tyramide® (TSA kit ...
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bioRxiv - Cell Biology 2022Quote: ... After 16 h 50 ng/ml of recombinant human (rh)TGFA (Sigma-Aldrich) and/or Afatinib (5 µM ...
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bioRxiv - Cell Biology 2024Quote: ... After 1 h in blocking solution (5% milk in TBS-T, Sigma-Aldrich), membranes were incubated in primary antibody solution (1% milk in TBS-T ...
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bioRxiv - Cancer Biology 2024Quote: ... After blocking nonspecific binding sites for 1 h using 5% dry milk (Sigma) in Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked for 1 h in 3 % BSA (Sigma-Aldrich, A7906-100G) in TBS-T at room temperature ...
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bioRxiv - Pathology 2023Quote: ... washed and fixed 8 h (RT) in 4% paraformaldehyde (P6148, Sigma Aldrich, USA). Tissues were embedded in Tissue-Tek OCT Compound (Sakura Finetek ...
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bioRxiv - Neuroscience 2023Quote: ... for 72 h in serum-free RPMI 1640 medium (SFM, Sigma Aldrich, R8758) supplemented with 2 mM Glutamax and 20 mM HEPES with pH adjusted to pH=7.6 (high pH) ...
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bioRxiv - Cell Biology 2023Quote: ... and stained for 1.5 h with anti-GFP antibody (1:500, Sigma, 290), followed by 1 h incubation with 1.4 nm nanogold-conjugated secondary antibody (1:60 ...
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bioRxiv - Cell Biology 2023Quote: ... coated with 10 μg/ml poly-L-lysine for 1 h (Sigma, P8920) and 10 μg/ml laminin for 5-10 minutes (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... replacement media after 1 h of infection contained 200 μg/ml AraC (Sigma) and was kept in the media until protein extraction8 ...
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bioRxiv - Immunology 2023Quote: ... cells were re-stimulated for 24 h with LPS 10 ng/mL (Sigma). In the in vivo model ...
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bioRxiv - Developmental Biology 2023Quote: ... The first series was stained with hematoxylin and eosin (H&E, Sigma Aldrich) for scoring ...
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bioRxiv - Microbiology 2023Quote: ... for 1 h and washed with HBSS (Sigma Aldrich, St. Louis, MO, USA). Stained samples were imaged with an LSM700 laser scanning confocal microscope (Carl Zeiss ...
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bioRxiv - Cell Biology 2023Quote: ... 6 µm sections were stained with hematoxylin and eosin (H&E, Sigma-Aldrich) or processed for immunohistochemical analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... or 1 h with horseradish peroxidase-conjugated anti-β-actin antibody (Sigma, A3854) diluted 1:20000 in 5% non-fat milk in TBST ...