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Citations for Millipore Sigma :
851 - 900 of 2153 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Samples taken from culture 12 h after fertilization were stained by Giemsa solution (Sigma, cat# GS80). Each conversion rate was calculated as the number of mature ookinetes (stage V ...
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bioRxiv - Cancer Biology 2021Quote: ... Positive control of ROS consisted in cells treated 1 h with 500 µM H2O2 (Sigma, H1009) before staining ...
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bioRxiv - Cell Biology 2020Quote: ... All cell lines were transduced with viruses for 24 h with 8 μg/ml polybrene (Sigma). Virus-infected cells were selected and grown in medium with 2 μg/ml puromycin or 200 μg /ml G418.
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bioRxiv - Cell Biology 2021Quote: ... 12 h fasting animals were given a bolus intraperitoneal injection of 20% D-glucose solution (SIGMA) in saline at 2 mg/g bodyweight ...
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bioRxiv - Biochemistry 2022Quote: ... and then incubated for 1 h in Earle’s Balanced Salt Solution (EBSS; Sigma-Aldrich Cat# E2888). Where indicated ...
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bioRxiv - Molecular Biology 2022Quote: ... at 37 °C for 1 h and followed by alkylation with 20 mM iodoacetamide (Sigma, USA) at 37°C in the dark for 30 mins ...
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bioRxiv - Bioengineering 2022Quote: ... The antibodies used were a) purified CHO h-IgG b) IgG from human serum (Sigma-Aldrich, resuspended in deionised H2O and stored at 4°C) ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 h at RT and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for 1 min ...
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bioRxiv - Microbiology 2020Quote: ... membranes were incubated for 1 h with a monoclonal Anti-FLAG-Peroxidase antibody (Millipore Sigma, #A8592) at a 1:5,000 dilution in PBST with 5% milk ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% heat-inactivated human AB plasma from healthy donors (SIGMA, male AB, H-4522), 1% l-glutamine ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% heat-inactivated human AB plasma from healthy donors (Sigma, male AB, H-4522), 1% l-glutamine ...
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bioRxiv - Neuroscience 2021Quote: ... for 48 h at 4°C and detected using diaminobenzidine (DAB) and nickel ammonium sulfate (Sigma) as chromogens ...
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bioRxiv - Immunology 2020Quote: ... BMDMs were infected with IAV (MOI, 10) for 2 h in serum-free DMEM media (Sigma). After 2 h of infection ...
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bioRxiv - Physiology 2020Quote: ... Sections were soaked for 10 s in Hoechst solution H 33342 (Sigma-Aldrich, B2261, 1:1000) and washed once with PBS before mounting with antifading Fluoromount G medium (Interchim ...
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bioRxiv - Plant Biology 2020Quote: ... they were incubated for 3 h in the presence of 30 µM MG132 (Sigma Aldrich #M7449). Then ...
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bioRxiv - Neuroscience 2021Quote: ... WT neurons were treated for 24 h with APOE2 protein (5 μg/ml, #SRP4760, Sigma-Aldrich), APOE4 protein (5 μg/ml ...
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bioRxiv - Plant Biology 2020Quote: ... After at least one h incubation in darkness with 200 μM acetosyringone (Sigma-Aldrich, Taufkirchen, Germany), 5 - 6 weeks old N ...
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bioRxiv - Physiology 2021Quote: ... Insulin concentrations after the 16 h hyperinsulinemia treatment were determined using human insulin RIA kit (Millipore). To mimic starvation ...
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bioRxiv - Immunology 2021Quote: ... then overnight at 37°C for 16 h under shaking in Tryptic Soy Broth (Sigma Aldrich) with kanamycin for Ba Δomp25 until the OD (OD at 600nm ...
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bioRxiv - Genetics 2020Quote: 293T/TLR cells were maintained in Dulbecco’s Modified Eagle’s Medium-High Glucose (DMEM-H) (Sigma-Aldrich) with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... for 2 h at 25,000 rpm (80,000 x g) over a 20% sucrose cushion (Sigma-Aldrich). Following centrifugation ...
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bioRxiv - Synthetic Biology 2021Quote: Approximately 48 h after transfection the cells were collected in 60 μL Accutase solution (Sigma-Aldrich). The fluorescence was measured on a Beckman Coulter CytoFLEX S flow cytometer using the 488 nm laser with a 525/40+OD1 bandpass filter ...
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bioRxiv - Immunology 2021Quote: ... Polymorphonuclear neutrophils (PMNs) were stimulated for 4 h with 100 nM PMA (Sigma-Aldrich, #P8139-10MG) or conditioned media from SARS-CoV-2-infected epithelial cells ...
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bioRxiv - Developmental Biology 2021Quote: ... followed 46–48 h later by 5 IU human chorionic gonadotropin (Sigma Aldrich, St. Louis, MO). The superovulated females were also bred overnight to vasectomized males for consistency across both treatment groups ...
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bioRxiv - Cell Biology 2021Quote: ... for 16 h or treated with vehicle (DMSO) or 2 μg/mL tunicamycin (Sigma-Aldrich, T7765) for 4 ...
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Splicing variation of BMP2K balances endocytosis, COPII trafficking and autophagy in erythroid cellsbioRxiv - Cell Biology 2020Quote: ... K562 cells were also treated for 48 h with 75 nM Bafilomycin A1 (B1793, Sigma-Aldrich) to assess autophagic flux.
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bioRxiv - Cell Biology 2021Quote: ... The immune complexes were collected by incubation (2 h, 4°C) with protein G-agarose (Sigma). Co-IP assays were performed by using Pierce™ Protein G Agarose (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... HBe-HA producing cells were treated for 4 h with Brefeldin A (5μg/mL, Sigma-Aldrich) and harvested in lysis buffer containing 50 mM Hepes ...
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bioRxiv - Cell Biology 2021Quote: ... Bis-benzamide H 33342 trihydrochloride (Hoechst) and propidium iodide (PI) from Sigma (St. Louis, MO, USA). MitoTracker Green ...
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bioRxiv - Cancer Biology 2021Quote: ... The tissue sections were stained with hematoxylin [Vector Laboratories #H-3401] and eosin (Sigma Aldrich #SLBH6215V). For immunohistological staining ...
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bioRxiv - Bioengineering 2022Quote: ... Mesenteries were immediately incubated for 1 h in sodium azide (NaN3, Sigma-Aldrich, S2002, lot BCBL9058V) diluted in phosphate buffered saline prepared in our laboratory (PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... in PBS for 4 h and then exposed to 100 µL dimethyl sulfoxide (DMSO; Sigma-Aldrich). Cell viability was measured by absorption at 570 nm in a microplate spectrophotometer (Spectromax 250 plate reader) ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated (1 h, room temperature) with HRP-conjugated goat anti-Rabbit IgG (Sigma #A4914), sheep anti-mouse (Sigma #A5906 ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: ... Hematoxylin & Eosin (H&E) staining of pancreatic sections were performed according to the manufacturer’s protocol (Sigma). The islet area was quantified using the same using ImageJ software.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... For hematoxylin/eosin (H&E) staining slides were deparaffinized as above followed by hematoxylin (Sigma, GHS232) solution for 10 minutes followed tap water for 15 minutes and eosin (Merck ...
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bioRxiv - Molecular Biology 2022Quote: The tissues and explants were fixed for 24 h in 10% neutral-buffered formalin (Sigma-Aldrich) at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by a 4 h of incubation with protein A/G agarose beads (LOT: 3460992, Millipore). The purified DNA fragments bound by ATF3 were analyzed using qPCR ...
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bioRxiv - Neuroscience 2023Quote: ... the clarified lysate was treated with 50U/ml of benzonase (Novagen; 1 h at 37 °C) and centrifuged ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated 48 – 72 h with sheep anti TPH (1:1000, ab1541, Merck Millipore) primary antibody in carrier solution containing 1% NGS and 1% BSA in PBST at +4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... dialysis was performed for 12 h against the buffer A containing 50% glycerol (Sigma-Aldrich, USA). The His-tag of the recombinant protein was removed during 12 h of incubation with 1-2 units of the enteropeptidase (L-HEP ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were blocked for 1 h at room temperature in 1 x casein blocking buffer (Sigma) and immunoblotted using primary antibody to Claudin-2 (1:500 dilution ...
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bioRxiv - Genomics 2024Quote: ... Antibiotic selection was started 30 h after transduction by adding 1 μg/mL puromycin (Sigma, P8833) directly to the plates without changing the medium ...
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bioRxiv - Genomics 2024Quote: ... Antibiotic selection was started 30 h after transduction by adding 1 μg/mL puromycin (Sigma, P8833) directly to the plates without changing the medium ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability was determined at 24 h by staining cells with 2 μg/mL PI (Sigma) and AnnexinV (AnnV ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 h and then incubated at room temperature with 2µg/ml propidium iodide (Sigma, P4170) for 15 min prior to imaging with MetaXpress (Molecular Devices) ...
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bioRxiv - Cell Biology 2023Quote: Brains of L3 larvae (∼120 h after egg laying) were dissected in Schneider’s media (Sigma Aldrich) containing 10% foetal calf serum (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Cells were treated for 24 h with DMSO as vehicle or inhibitors: hemicholinium-3 (Sigma-Aldrich), RSM-932a (Cayman Chemicals) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated 24 h prior to exposure to 70-kDa FITC-dextran (Millipore Sigma, 46945). 3 h before the dextran treatment ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were stained for 1 h at RT with antibodies against vinculin (hVin1, Sigma; 1:400) and either (phosphoTyr397-FAK (Cat# 44-625G ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 h in the dark at RT and counterstained with DAPI (1:1000, Sigma, T7024). Finally ...