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Citations for Millipore Sigma :
6401 - 6450 of 10000+ citations for Recombinant Human SERPINF1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The expression of proteins was induced with tetracyclin (1 µg/mL; Sigma-Aldrich, T7660). Cell were treated with actinomycin D (10 ng/mL ...
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bioRxiv - Cell Biology 2024Quote: PLA in cultured PAEC were performed using Duolink PLA protein detection technology (Millipore Sigma) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Membranes were stained for total protein using Ponceau S (Sigma-Aldrich; St. Louis, MO) and subsequently probed with Aldehyde dehydrogenase 1-A1 ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein concentration was measured using a Bradford assay (Sigma-Aldrich/Millipore B6816-500ML) as per manufacturer’s instructions in optically clear 96-well plate (VWR 62402-933 ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein concentration was measured using a Bradford assay (Sigma-Aldrich/Millipore B6816-500ML) as per manufacturer’s instructions in optically clear 96-well plate (VWR 62402-933 ...
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bioRxiv - Molecular Biology 2023Quote: ... separated by SDS-PAGE and proteins transferred to polyvinylidene difluoride membrane (Immobilon-P, Millipore). Membranes were blocked in 5% milk in PBS/ 0.1% Tween-20 ...
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bioRxiv - Molecular Biology 2023Quote: RIP was performed using a Magnetic RIP RNA-Binding Protein Immunoprecipitation (Millipore, 17-700) by the manufacturer’s guidelines ...
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bioRxiv - Biophysics 2023Quote: ... GST-tagged Elf1 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Glutathione Sepharose 4 Fast Flow resin (GE Healthcare) ...
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bioRxiv - Immunology 2023Quote: ... and protein complexes were eluted with 150 mg/ml of 3X FLAG peptide (Sigma). Samples were diluted in 2X SDS sample buffer and analyzed with SDS–PAGE according to standard procedures ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of proteins were incubated overnight with the anti-FLAG M2 (Sigma M8823) or anti-HA (Pierce #88836 ...
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bioRxiv - Microbiology 2023Quote: ... followed by protein precipitation after the addition of trichloroacetic acid (TCA, Sigma-Aldrich, 91228), (v:v ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were resolved in 10% SDS/PAGE and transferred to nitrocellulose membranes (Sigma Aldrich). Membranes were probed for 2 h at RT (or overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by SDS-PAGE and transferred to polyvinyl difluoride (PVDF) membranes (Millipore) according to standard protocols ...
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bioRxiv - Biochemistry 2024Quote: ... Elution step of enriched proteins was performed using 2.25 M ammonia solution (Sigma, #5.33003) for 16 h ...
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bioRxiv - Biochemistry 2024Quote: ... The antibody-lysate complex was incubated with protein A- conjugated sepharose beads (Sigma #P3391) and eluted in 2X laemelli dye ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were precipitated by adding 100 μl of gamma-globulins (10 mg/ml; Sigma) and 2 ml of 20% TCA followed by centrifugation at 2,000 r.p.m ...
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bioRxiv - Developmental Biology 2023Quote: ... supernatant containing protein suspension was collected and quantified with Bradford reagent (Sigma, B6916-500ML) at the spectrophotometer ...
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bioRxiv - Immunology 2023Quote: ... bound proteins were eluted in TBS buffer supplemented with 5mM d-desthiobiotin (Sigma Aldrich). Affinity-purified proteins were incubated with HRV-3C protease to remove purification tags and subsequently purified by size-exclusion-chromatography on a Superose 6 Increase column (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... secondary antibodies were added and the proteins were detected using an ECL kit (Millipore). Immunoreactive signals were visualized using the GE Amersham Imager 600 chemiluminescence system.
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bioRxiv - Neuroscience 2023Quote: ... PLP (Anti-Myelin Proteolipid Protein Antibody, CT, clone PLPC1 MAB388 Sigma-Aldrich, 1:200), MHCII (Dako 1:50) ...
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bioRxiv - Plant Biology 2024Quote: ... The separated proteins were transferred to Immobilon®-P PVDF membrane (Merck Millipore, USA) and probed with primary antibodies overnight ...
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bioRxiv - Systems Biology 2024Quote: ... Tagged proteins were detected using mouse α-Flag M2 (Sigma Aldrich F3165, 1:10,000), mouse α-V5-tag (Thermo Fisher R960-25 ...
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bioRxiv - Microbiology 2024Quote: Incubation mixtures (30 µl) contained 10 µM of protein and 5× SYPRO Orange (Sigma) in 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Plant Biology 2024Quote: ... The eluted protein was further concentrated using a spin concentrator (10 kDa Amicon, Millipore) and rebuffered in 20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: Protein content in mitochondria-enriched fractions were estimated using Bradford Assay reagent (Sigma-Aldrich) followed by solubilisation as previously reported by Rawat et al21 ...
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bioRxiv - Genetics 2024Quote: ... proteins on the SDS-PAGE were transferred to a PVDF membrane (Millipore, Billerica, MA) at 100V for 3 hours at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Purified His-tagged proteins were concentrated and desalted using Amicon centrifugal filters (Millipore, UFC500396) in TBS buffer and stored in storage buffer (30 mM Tris–HCl ...
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bioRxiv - Pathology 2024Quote: Protein samples were extracted using radioimmunoprecipitation buffer containing inhibitors of proteases (cOmplete, Sigma-Aldrich) and phosphatases (PhosSTOP ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... protein levels was done using heparin affinity columns (Sigma-Aldrich, St. Louis, MO, USA) and Western blot analysis as previously described [37 ...
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bioRxiv - Biophysics 2024Quote: ... Protein was initially purified using affinity chromatography using HIS-Select Cobalt Affinity Gel (Sigma) on a gravity flow column ...
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bioRxiv - Bioengineering 2024Quote: ... After dialysis the protein was concentrated through Amicon centrifugal filters (Millipore, Burlington, MA, USA) with a molecular weight cutoff of 3 kDa ...
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bioRxiv - Biophysics 2024Quote: ... The final concentration of the protein as estimated from the BCA test (Sigma Aldrich) was 8.4 mg/ml.
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bioRxiv - Cell Biology 2024Quote: ... Solubilized protein was added to pre-equilibrated anti-FLAG agarose affinity resin (Sigma, A2220) and incubated for 90min at 4°C on a rotating wheel ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were reduced with 20 mM DL-Dithiothreitol (DTT; BioUltra, for molecular biology, Sigma) at 56°C for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... was subjected to affinity purification using S-protein-agarose (Merck Millipore, Burlington, MA, U.S.A.) for 1– 2 h and washed 4 times ...
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bioRxiv - Microbiology 2024Quote: ... purified RSP protein was mixed with Biotin-NHS (Biotinamidocaproate N-hydroxysuccinimide ester, Sigma Aldrich) and three rounds of MACS (with RSP concentrations of 100 nM (the first one ...
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bioRxiv - Plant Biology 2024Quote: ... Purified proteins were concentrated using 10 kDa ultra-centrifugal filter Amicon (Merck Millipore, Germany). The quality and quantity of proteins were analyzed by SDS-protein gel electrophoresis (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... Protein precipitates were solubilized in 6M urea and subsequently quantified using BCA kit (Sigma). Ten micrograms of the protein samples were reduced with 10mM dithiothreitol (DTT ...
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bioRxiv - Biophysics 2024Quote: ... Protein bands were transferred by tank transfer onto a polyvinylidene difluoride (PVDF, EMD Millipore) membrane in transfer buffer (25 mM Tris pH 8.3 ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to a polyvinylidene difluoride immobilon TM-P membrane (Millipore, Burlington, MA) overnight at 4°C and blocked in 5% nonfat powdered milk in TBS with Tween 20 (pH 7.6 ...
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bioRxiv - Biochemistry 2024Quote: ... 100 mM Tris pH 8.5 with 800 ng of yeast enolase protein (Sigma-Aldrich) added as an internal protein control ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were resolved by SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). Immunoblots were developed using Western Lightning Chemiluminescence Reagent Plus (Advansta ...
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bioRxiv - Cell Biology 2024Quote: ... Cleaved proteins in the supernatant were concentrated with a 100K (MWCO) concentrator (EMD Millipore) to 500 μL and purified via SEC on a TSKgel G4000SWXL column (TOSOH Bioscience ...
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bioRxiv - Physiology 2024Quote: ... The membrane with transferred protein was stained with Ponceau S stain (Sigma-Aldrich #P7170) to check the quality of transfer and protein load ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein bands were visualised by adding Immobilon Forte Western HRP substrate (Millipore #WBLUF0100) on a ChemiDoc XRS+ (BioRad).
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bioRxiv - Neuroscience 2024Quote: ... The Syt1 and Syt7 proteins were further treated with Benzonase (Millipore Sigma, Burlington, MA) at room temperature for 1 hr with Syt1 additionally being run through ion exchange (Mono S ...
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bioRxiv - Biochemistry 2024Quote: ... 100 mM Tris pH 8.5 with 800 ng of yeast enolase protein (Sigma-Aldrich) added as an internal protein control ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were incubated with 20μl α-V5 or α-FLAG M2 resins (Sigma-Aldrich) for 4 hours at 4°C to pull down CLK or CK1α ...
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bioRxiv - Neuroscience 2024Quote: ... and immunoreactive proteins were visualized using Immobilon® Forte Western HRP Substrate (Merck Millipore) at the Imager Chemi Premium (VWR International) ...