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Citations for Millipore Sigma :
6351 - 6400 of 10000+ citations for Recombinant Human SERPINF1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and incubated with antibodies against OL markers proteins including Olig2 (MABN50, 1:500 Millipore, CA ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were concentrated by ultrafiltration on 10K Amicon® Ultra Centrifugal Filters (Merck Millipore), aliquoted ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then reduced by 10 mM tris(2-carboxyethyl)phosphine (TCEP, Millipore Sigma) for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: Total proteins were extracted from organoid cell pellets using 1X RIPA buffer (Sigma Aldrich) supplemented with 1X Halt™ protease and phosphatase inhibitor cocktail (Pierce) ...
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bioRxiv - Microbiology 2023Quote: ... Sized proteins were concentrated to >50 mg/ml using 10 kDa MWCO concentrators (Millipore), flash frozen on liquid nitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted protein was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Millipore-Sigma) centrifuged at 4,000 x g to no more than 3 ml ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted protein was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Millipore-Sigma) centrifuged at 4,000 x g to no more than 3 ml ...
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bioRxiv - Immunology 2023Quote: ... protein bands on the blots were developed with chromogenic substrate SigmaFast BCIP/NBT (Sigma).
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bioRxiv - Biophysics 2023Quote: ... Resolved proteins were then electrophoretically transferred onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, USA) at 90 V for 2.5 h ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was discarded and the protein pellet was washed using guanidine hydrochloride (Millipore, #1.04220.0100 ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were filtrated using a 96-well filter plate (Millipore, Burlington, MA, USA) to remove precipitated protein and the A280 was remeasured before starting the binding assay ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein was then transferred to 0.4 μm polyvinylidene fluoride (PVDF, Merck Millipore) membrane ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred onto a PVDF membrane (Immobilon-P, Merck Millipore, Massachusetts, USA) and the membranes were blocked with PBS containing 5% non-fat dry milk for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by 10% SDS-PAGE gel and transferred onto PVDF membranes (Millipore). After blocked in 5% skim milk for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: Total protein from cortical organoids and HLCs were isolated using RIPA buffer (Sigma-Aldrich) consisting of protease and phosphatase inhibitors (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... Protein lysates were collected from cells using ice cold RIPA buffer (R2078, Sigma Aldrich) with HaltTM 100x protease inhibitor cocktail added (78440 ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentrations were normalized with Milliplex® MAP Assay Buffer (EMD Millipore, Billerica, MA) to 6 μg protein per 12.5 μL for cytokine analysis and 1 μg protein per 12.5 μL for the MAPK pathway analysis ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were eluted using 10 mM glutathione or 0.5 mg/mL 3XFLAG peptide (Sigma) in Lysis Buffer with 2 mM DDM.
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bioRxiv - Plant Biology 2023Quote: ... Expression of fusion proteins was verified by immunoblotting using either mouse anti-LexA (Sigma) or rat anti-hemagglutinin (HA ...
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bioRxiv - Biochemistry 2023Quote: ... Cleaved proteins in the supernatant were concentrated with a 100K MWCO concentrator (EMD Millipore) to 500 µl and purified via size exclusion chromatography on a TSKgel G4000SWXL column (TOSOH Bioscience ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant containing cleaved proteins was concentrated using a 30kDa MWCO concentrator (EMD Millipore) to 1mL ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were separated and transferred to Immobilon-P PVDF membrane (0.45 μm, Millipore Sigma). The membrane was blocked with 5% bovine serum albumin for 1 h and incubated overnight at 4°C with various antibodies (1:500 dilution for the PIKfyve antibody and 1:1000 dilution for all other antibodies) ...
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bioRxiv - Biochemistry 2023Quote: ... Protein expression was performed in the presence of 3.5 mM valproic acid (Merck Millipore) and 10 μM kifunensine (Biomol) ...
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bioRxiv - Genomics 2022Quote: ... Proteins were transferred to a PVDF membrane (Millipore; Immobilon-FL, 0.45 mm pore size) in transfer buffer (25 mM tris ...
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bioRxiv - Biophysics 2023Quote: ... then the proteins were stained with 0.22% Coumassie brilliant blue G-250 (Sigma, USA) diluted in the solution F ...
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bioRxiv - Bioengineering 2023Quote: ... then resuspended in 100 µL protein storage buffer: 4 M urea (Sigma-Aldrich, U5128), 1 % SDS (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were probed with 1:2000 chicken anti-LDL primary antibody (#GW20089F, Sigma-Aldrich) diluted in PBS-T overnight at 4°C and detected with 1:2000 HRP-conjugated goat anti-chicken secondary antibody (#A16054 ...
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bioRxiv - Cell Biology 2022Quote: ... For Chemiluminescence westerns: The proteins were electro-transferred to PVDF membranes (0.45 μm, Millipore), and then the total protein was stained using Ponceau (Thermo Fisher Scientific) ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... Protein transfer was performed on methanol-activated Immobilon®-FL PVDF membrane (Sigma-Aldrich). Membrane was blocked for 1h in 5% BSA (bovine serum albumin ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg of pure protein emulsified in Freund’s incomplete adjuvant (Sigma Aldrich, Taufkirchen, Germany) was injected subcutaneously to six weeks old female NMRI mice (Charles River Laboratories ...
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bioRxiv - Immunology 2023Quote: ... proteins were transferred to a nitrocellulose membrane and stained with Ponceau S (Sigma Aldrich) to monitor protein loading ...
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bioRxiv - Biochemistry 2023Quote: ... Expression of chaperone protein was induced with 1 μg/ml tetracycline (Sigma-Aldrich, T7660).
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bioRxiv - Biochemistry 2023Quote: ... Unbound protein was then concentrated in a 30 kDa MWCO Amicon concentrator (Sigma Millipore) and then loaded onto a 24 mL Superdex 200 10/300 GL (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... Unbound protein was then concentrated in a 30 kDa MWCO Amicon concentrator (Sigma Millipore) and then loaded onto a 24 mL Superdex 200 10/300 GL (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: ... protein expression was induced by adding 0.5 mM isopropyl β-Dthiogalactopyranoside (IPTG; Sigma Aldrich). After 20h incubation at 15°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were detected with a streptavidin-HRP conjugate (Millipore-Sigma, Cat#: OR03L-200UG), used at 1:2,000 dilution (see Reagent Table).
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bioRxiv - Biochemistry 2023Quote: ... the protein was concentrated using a centrifugal filter (Amicon Ultra, MWCO 30 kDa, Sigma) to 41.46 mg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was concentrated with an Amicon Ultra-4 (Merck Millipore, MWCO 3 K) to a concentration of 726 µM ...
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bioRxiv - Developmental Biology 2023Quote: ... Then proteins were transferred onto a 0.45-μm polyvinylidene fluoride membrane (Sigma Aldrich, IEVH00005) and the membranes were blocked in TBST containing 5% skimmed milk for 1 h at room temperature and then incubated with the indicated primary antibodies overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... GFP-V5 or GFP-FLAG fusion proteins using OptiMem and Fugene HD (Sigma-Aldrich). Forty-eight hours later ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were eluted twice with 2 μg/μl V5 peptide in water (Sigma-Aldrich) or 1 μg/μl 3x FLAG peptide (ApexBio ...
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bioRxiv - Cancer Biology 2023Quote: ... Separated proteins were then transferred to Immobilon®-P PVDF Membranes (Milipore Sigma #ISEQ00010) using the BioRad Transblot SD Semi-dry Transfer Cell as per manufacturer’s recommendations.
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced for 10 mins at 60°C with 10 mM dithiothreitol (Sigma) in 25 mM ammonium bicarbonate and then alkylated with 55 mM iodoacetamide (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... The resolved proteins were transferred to an Immobilon-P polyvinylidene difluoride (PVDF) membrane (Millipore), and the membranes were incubated with Tris-buffered saline with Tween 20 (TBST ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were similarly incubated with HA binding protein (HABP) (EMD Millipore 385911, Burlington, MA), then detected with an appropriate fluorescent secondary antibody for 1 h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins separated by SDS-PAGE were transferred to PVDF membranes (Millipore, Cat#PVH00010). After blocking with 5% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were then wet transferred to a polyvinylidene difluoride (PVDF) membrane (IPVH00010, Sigma Aldrich). The membrane was incubated in blocking buffer consisting of 5% milk in PBS plus 0.1% Tween-20 ...
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bioRxiv - Biochemistry 2023Quote: ... The interaction of the proteins was quantified after adding the substrate phenylenediaminedihydrochloride (Sigma Aldrich) plus H2O2 by measuring the resulting absorbance at 492 nm in the Multiskan SkyHigh Spectrophotometer (ThermoFisher Sientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were concentrated using AmiconUltra (M.W. 3,000 Da; Sigma-Aldrich, St. Louis, MO, USA) and stored at −80 °C before use.