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Citations for Millipore Sigma :
6301 - 6350 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were screened using ELISA (see below) and antigen-reactive clones were expanded in serum IgG free RPMI-1640 (Sigma-Aldrich, MO, USA) into roller bottles (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were subjected to SDS-PAGE and transferred to a PVDF membrane (Millipore) for Western blot analysis probing for HA and FLAG epitopes using 1° antibody = 1:500 mouse αHA (Roche) ...
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bioRxiv - Immunology 2021Quote: ... membranes were stripped in 1x ReBlot Plus Strong Antibody Stripping Buffer (Millipore 2504) for 15 minutes before blocked again for reblotting.
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bioRxiv - Immunology 2020Quote: ... The lung and SCN slices were cultured individually on a Millicell membrane (Millipore) in a Petri dish in supplemented DMEM containing 1µM luciferin (Promega) ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were transferred to an Immobilon-P PVDF Membrane (Sigma-Aldrich; Cat #: IPVH) and blocked in PBS/0.1%Tween20/5% Blotting-Grade Blocker (BioRad ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were run on SDS-PAGE and transferred to PVDF-FL membranes (Millipore) as previously published (Michele et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... membranes were spotted with 0 to 200 μM of PA (Sigma P-9511) or PS (Sigma P-6641 ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were stained with the membrane lipid marker PKH67 (Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... SDS-PAGE gels were transferred to a PVDF membrane (Immobilon-FL; EMD Millipore) for 30 minutes per gel at a constant 240 mA ...
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bioRxiv - Biophysics 2022Quote: ... and thereafter the membranes were mounted to glass slides with Mowiol (Sigma-Aldrich) and stored protected from light at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... the membranes were washed three times with phosphate-buffered saline (PBS, Sigma-Aldrich) and stored in PBS at 4°C until further use ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were placed on a 30 mm porous membrane (Millipore, Billerica MA, USA) and kept in 100 mm diameter Petri dishes filled with 5 ml of culture medium containing 25% heat-inactivated horse serum ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was then pre- hybridized 30 min with PerfectHyb buffer (Sigma-Aldrich). The radioactively labeled probe was first prepared with γ[32 P]ATP ATP and DNA oligonucleotides listed in Supplementary Table S5 with a T4 polynucleotide kinase kit (Promega) ...
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bioRxiv - Bioengineering 2022Quote: ... followed by filtration through a 0.45 μM membrane (Millipore, Boston, MA, United States). Pseudoviruses were concentrated by centrifugation at 25000 rpm for 2 h at 4◦C ...
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bioRxiv - Cell Biology 2022Quote: ... separated by 15% SDS-PAGE and transferred to Immobilon-P PDVF membranes (Millipore). Membranes were blocked with TBST (20 mM Tris ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We sealed plates with BreatheEasy membranes (Millipore-Sigma, Diversified Biotech, Dedham, MA, USA) to prevent microbial contamination and cross-contamination ...
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bioRxiv - Genomics 2020Quote: ... and 10 µl of paraformaldehyde previously filtered through 0.22 μm PES membranes (Millipore). Three fields per sample were observed and quantified with Olympus FV1000 Multiphoton Confocal Microscope ...
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bioRxiv - Systems Biology 2019Quote: ... All eluents were filtered through a 0.22 µm Durapore membrane (EMD Millipore Corporation) and stored at 4°C until use ...
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bioRxiv - Microbiology 2019Quote: ... viral suspensions were concentrated using a 100 kDa cut-off PES membrane (Millipore) and the retentate containing the bacteriophages (>100 kDa ...
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bioRxiv - Cell Biology 2020Quote: ... and subjected to western blotting via wet transfer to 0.45µm nitrocellulose membrane (Millipore). Ten µg total lysate were loaded (corresponding to 1% ...
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bioRxiv - Immunology 2019Quote: ... The rSsEno-containing supernatant was filtered through a 0.45 μm nitrocellulose membrane (Millipore) and further subjected to Ni2+-affinity chromatography in buffer A ...
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bioRxiv - Neuroscience 2019Quote: ... and transferred to a polyvinylidene difluoride membrane (PVDF; Merck Millipore Ltd., Tullagreen, Ireland) that was blocked in Licor Blocking Buffer diluted 1:1 in 0.1M PB ...
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bioRxiv - Biochemistry 2019Quote: ... the membrane was developed using Luminata Classico Western HRP substrate (Merck Millipore, USA) and detected in ImageQuant LAS500 imager (GE Life Science ...
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bioRxiv - Biochemistry 2019Quote: ... and then transferred to an Immobilon-FL PVDF membrane (EMD Millipore, Burlington, MA). REVERT Total Protein Stain was used according to the standard protocol to quantify lane loading ...
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bioRxiv - Neuroscience 2019Quote: ... and electro transferred to a polyvinylidene difluoride (PVDF) membrane (Merck Millipore, Burlington, EUA). After blocking with 5% bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2020Quote: ... membranes were subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP Substrate (Millipore) using a Gel-Doc XR+ System (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... Membranes were blocked in 0.1 % Tween PBS supplemented with 5% skimmed milk (Sigma) and subjected to immunoblotting with the following primary antibodies at the indicated dilutions ...
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bioRxiv - Neuroscience 2019Quote: ... mounted on nitrocellulose membrane (Type AAWB, 0.8 micron pore size, Millipore, Burlington, MA) and fixed by immersion in 4% paraformaldehyde in PBS for ~1 hour ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were subjected to SDS PAGE electrophoresis and transferred onto PDVF membrane (Millipore). Membranes were probed with antibodies against YAP (Cell Signaling Technology 4912) ...
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bioRxiv - Microbiology 2019Quote: ... Samples resolved on SDS-PAGE and transferred onto PVDF membrane Immobilon-P (Millipore) using a standard liquid transfer protocol ...
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bioRxiv - Cell Biology 2019Quote: ... and separated by electrophoresis and transferred to a PVDF membrane (Immobilon-P, Millipore). The membranes were blocked in 5% non-fat milk dissolved in Tris buffered saline with 0.05% Tween-20 (TBS-T ...
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bioRxiv - Physiology 2019Quote: ... The membranes were blocked with 5 % nonfat dry milk or 5 % BSA (Sigma) for 1 h at room temperature and incubated with the antibodies described in the reagents section ...
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bioRxiv - Microbiology 2021Quote: ... the membranes were incubated for 2 minutes with ECL (Immobilon Western, WBKLS0500, Millipore) and chemiluminescence recorded on a ChemiDoc MP imaging system (Biorad).
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bioRxiv - Microbiology 2021Quote: ... then transferred to an Immobilon-FL PVDF membrane (Merck Millipore Ltd., Tullagreen, Ireland) using a Trans-Blot® Turbo™ Transfer System (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked overnight in TBS containing 5% Bovine Serum Albumin (Sigma Aldrich). After three 10-minute washes with TBST (0.1% tween 20 ...
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bioRxiv - Bioengineering 2020Quote: ... This was followed by dialysis (cellulose membrane 14 kDa cut off; Sigma-Aldrich) against MQ-water at 4°C for 5-7 days ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were loaded in 10% polyacrylamide gels and transferred to Immobilon membranes (Millipore) for Western blot ...
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bioRxiv - Biochemistry 2020Quote: ... the membranes were developed with an Immobilon Western Chemiluminescent HRP Substrate (Millipore, MA). The membrane was exposed and analyzed using ChemiDoc MP Image analyzer (BIO-RAD ...
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bioRxiv - Biophysics 2021Quote: ... The quenched reaction was immediately filtered through a 0.22μm filter membrane (Millipore Sigma) and washed three times with 2mL quenching buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then washed and developed with Crescendo chemiluminescence substrate system (Sigma, WBLUR0500). Signals were detected using a Fusion Solo S imaging system (Vilber) ...
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bioRxiv - Microbiology 2021Quote: ... concentrated and desalted using Ultrafree-15 Biomax-100 membrane centrifugal filter units (Millipore) in 10 mM Tris pH 7.9 ...
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bioRxiv - Molecular Biology 2021Quote: ... with 120V electrophoresis for 90 min and blotted onto a nitrocellulose membrane (Millipore). The membrane was blocked with 5% fat-free milk/PBS at RT for 1 h ...
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bioRxiv - Biophysics 2021Quote: ... 10 mM L-arginine with 0.5 % (v/v) membrane protease inhibitors (Sigma-Aldrich) and 1.5 % (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... and blotted onto a Amersham Protran Premium Western blotting nitrocellulose membrane (Sigma, #GE10600003). The nitrocellulose membrane was blocked with Pierce Clear Milk blocking buffer (ThermoFisher ...
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bioRxiv - Neuroscience 2020Quote: ... and explanted onto a Millicell-CM membrane (0.4-µm pore size; Millipore, MA). The membranes were then placed in 750 µL of MEM culture medium ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were routinely stained with Naphtol blue black (Amido-black; Sigma- Aldrich 3393) to verify the transfer and loading ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were subjected to SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked with 5% nonfat milk for 1 h at room temperature and then probed with the indicated primary antibodies at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... Culture supernatant was filter-sterilized with 0.22 µm membrane (Steriflip®, EMD Millipore) under gentle vacuum ...
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bioRxiv - Microbiology 2020Quote: ... Samples were individually vacuum filtered using 0.025 μm membrane filters (VSWP01300, MF-Millipore) that were prewashed with 500 μL 70% ethanol ...
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bioRxiv - Neuroscience 2019Quote: ... Pre-Cast 4-20% gradient gel (Bio-Rad #456-1096 PVDF membrane (Millipore, Immobilon-FL Transfer Membranes IPFL00010 ...