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Citations for Millipore Sigma :
6251 - 6300 of 10000+ citations for Mouse Anti Rubella virus Glycoprotein E1 1715 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and NHA cells were transduced with equal titers of concentrated virus in complete growth media supplemented with 1 μg/ml polybrene (Sigma) for 24 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... 75 μL of concentrated virus were added in 2 mL of medium in presence of 8 μg/mL of polybrene (Sigma) for a 60-mm plate ...
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bioRxiv - Neuroscience 2023Quote: ... The media containing lentivirus was collected and virus particles were concentrated 20- fold using Amicon Ultracel-100k (EMD Millipore 901024). For all the experiments ...
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bioRxiv - Neuroscience 2023Quote: ... Culture supernatant containing the virus was collected 3 days after transfection and 20-fold concentrated using Amicon Ultra 15 10K (Millipore) centrifugal filter ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants containing purified virus were applied onto HeLa cells for 48 h in the presence of 8 μg/mL polybrene (Sigma). Following transduction ...
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bioRxiv - Cancer Biology 2023Quote: ... 786-O cells were cultured with virus containing media and 4µg/mL polybrene (Sigma Aldrich, Cat. No. TR-1003-G) for 24 hours ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... Lentivirus transduction was performed by spinoculation at 1200 X g for 2 hours at 32° C in the presence of virus particles and 8 ug/ml polybrene (Millipore). The next day ...
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bioRxiv - Cell Biology 2023Quote: ... 600,000 cells were seeded into each well of a 6-well plate and one week later were infected with up to 100ul virus per well (and 8 ug/ml polybrene (TR-1003-G, Sigma)) overnight ...
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bioRxiv - Microbiology 2023Quote: ... Viral titres were calculated by infecting PMA-treated THP-1 cells (2×105 cells/ml) or U87 cells (105 cells/ml) with dilutions of virus in the presence of polybrene (8 μg/ml, Sigma) for 48 h and enumerating GFP-positive cells by flow cytometry using the FACS Calibur (BD) ...
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bioRxiv - Cancer Biology 2023Quote: ... The tumor cell lines and UPCs were infected with the concentrated virus in a culture media containing 8 µg/ml Polybrene (Sigma) and positively transduced cells selected by FACS for the expression of the corresponding fluorescent reporter.
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bioRxiv - Biophysics 2023Quote: ... The cells were then infected in triplicate with VSV-G-pseudotyped GFP-encoding virus in the presence of polybrene (10 µg/ml; Sigma) at room temperature for 20 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Fibroblasts were isolated from normal human skin and immortalized using human papilloma virus type oncogenes E6/E7 as previously described66 and cultured at 37 °C and 5% CO2 in DMEM (Sigma) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... fibroblasts were seeded and allowed to attach overnight before incubation with the virus and polybrene (8 µg ml−1, Sigma) for 24 h ...
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bioRxiv - Biochemistry 2024Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography ...
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bioRxiv - Neuroscience 2024Quote: ... The medium was refreshed the next day and virus-containing medium was collected and concentrated 72 hours post-refreshing using Amicon Ultra 15 filters (C7715, Merck Millipore).
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bioRxiv - Pathology 2024Quote: ... Huh7 infection was repeated and non-internalized virus particles were removed by adding 2 mg/ml of pronase (Sigma-Aldrich) in 200 µl of serum-free medium for 5 min on ice ...
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bioRxiv - Developmental Biology 2024Quote: ... The 54%-40% fraction containing the virus was collected using an 18-gauge needle and concentrated using an Amicon Ultra Centrifugal Filter Ultracel-100K (Millipore). The titer of each AAV (in vector genomes (VG)/ml ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation in either sheep anti-mouse or donkey anti-rabbit IgG-conjugated with horseradish peroxidase (HRP) (Millipore Sigma, GENA931, GENA934, 1:4,000) in TBST at room temperature for two hours ...
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bioRxiv - Cancer Biology 2020Quote: ... This was followed by incubation with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgGs or goat anti-mouse IgGs (both Sigma-Aldrich, USA; 1:10000). Protein bands were visualized using SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... diluted anti-YFP antibody or other specific antibodies followed a 1:10,000 (v/v) diluted horseradish peroxidase (HRP)-conjugated goat anti-rabbit or a goat anti-mouse antibody (Sigma-Aldrich, St. Louis, MO, USA). The detection signal was developed using the ECL substrate kit as instructed (Thermo Scientific ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were stained for bait proteins using mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody, Sigma-Aldrich, Cat# F3165; used at 1:2500), Rabbit-anti-Giantin antibody (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were subsequently incubated with peroxidase-conjugated secondary antibodies (anti-mouse IgG, HRP conjugate, EMD Millipore, AP160P; anti-rabbit IgG, HRP-linked, EMD Millipore, 12-348) at room temperature for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit anti-BUBR1-p620 (custom raised by Moravian, 1:1000) and mouse anti-α-Tubulin (clone B-5-1-2, T5168, Sigma-Aldrich, 1:10000). Secondary antibodies used were goat anti-mouse IgG HRP conjugate (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... The primary antibodies used are polyclonal goat anti-TrkB (AF1494, Bio-Techne Sales Corp; 1:500) and monoclonal mouse anti-α-tubulin (T5168, Sigma-Aldrich; 1:5000). The secondary antibodies used are peroxidase AffiniPure donkey anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... Western blots were treated with antibodies against the appropriate proteins followed by a second reaction with anti-mouse or anti-rabbit IgG conjugated to horseradish peroxidase (Sigma, St. Louis, MO, USA). The SuperSignal West Pico and Femto substrate kits (Pierce ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-phospho-c-Jun (Thr91, cell signal, 2303, 1:1000), rabbit anti-c-Jun (cell signal, 9165, 1:1000) and mouse anti-GAPDH (Sigma, Cat: G8795, 1:1000). Immunoreactivity was visualized using appropriate HRP-conjugated secondary antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... and rabbit anti-pSer502-NT5C2 (dilution 1:1000) (Covance) antibodies and β-actin with a mouse monoclonal anti-β-actin antibody (Sigma Aldrich, #A5441). We visualized the immunoblots using an Odyssey Infrared Imaging System (LI-COR Biosciences) ...
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bioRxiv - Plant Biology 2022Quote: ... and αTubulin levels were used as loading control (α_Tubulin antibody, Monoclonal Anti-α-Tubulin antibody produced in mouse, Sigma, Catalog number T5168; anti-Rabbit IgG, Sigma, Catalog number A0545).
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bioRxiv - Microbiology 2022Quote: ... and −7173 of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were washed with TBST buffer (three times, 7 minutes each) and incubated with anti-mouse-HRP or anti-rabbit-HRP (Sigma-Aldrich; 20,000 times dilution) for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... Endogenously expressed AGAL and tubulin were detected with rabbit monoclonal anti-galactosidase alpha antibody (used for staining of AGAL in kidney) and mouse monoclonal anti-acetylated tubulin antibody (T7451, SIGMA-Aldrich, Prague, Czech Republic), respectively.
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bioRxiv - Microbiology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2024Quote: ... tissue sections were exposed to anti-Iba1 rabbit antibody (FUJIFILM Wacko, 013-27691, 1:300 dilution) and anti-GFAP mouse antibody (Sigma, G3893, 1:300 dilution) for overnight at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The primary antibodies were detected by incubation with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit secondary antibodies (Sigma-Aldrich, A9044 and A5278), followed by incubation with the Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... They were then incubated overnight at 4°C with primary antibodies in blocking solution: rabbit anti-GFP (MBL Cat# 598; 1/300) and mouse anti-HA (Sigma, Cat# 12ca5; 1/50). On the next day ...
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bioRxiv - Microbiology 2024Quote: ... and -71 60 of anti-spike murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Neuroscience 2023Quote: ... secondary fluorescent antibodies were anti-mouse IgG (1:200, Chemicon, AP308F, the USA) conjugated with FITC and FITC anti-rabbit IgG (1:200, Sigma-Aldrich, F1262, the USA). To detect and measure the positive cells ...
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bioRxiv - Microbiology 2023Quote: ... of anti-spike murine antibodies (including cross-reactive mAbs against SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... primary antibody against α-tubulin (T5168) and secondary antibodies (A0545, anti-rabbit; A9044, anti-mouse IgG-peroxidase) were purchased from Sigma-Aldrich (Darmstadt, Germany).
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bioRxiv - Cell Biology 2022Quote: ... Antibodies used in this study are as follows: mouse anti-Flag M2 (F3165) and anti-Flag affinity gel (A2220) from Sigma Aldrich (St Louis, USA); rabbit anti-HSP70 (AF1663 ...
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bioRxiv - Neuroscience 2023Quote: ... horseradish peroxidase (HRP) conjugated secondary anti-rabbit IgG (cat. A0545) and anti-mouse IgG (cat. A9044) were procured from Sigma-Aldrich (St. Louis, MO). Rabbit polyclonal antibody to Iba1 (cat ...
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bioRxiv - Microbiology 2023Quote: ... and -71)60 of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924, RRID: AB_258426) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Cell Biology 2023Quote: ... secondary antibodies were applied (goat anti-rabbit IgG-Alkaline Phosphatase, A9919, Sigma Aldrich; goat anti-mouse IgG-Alkaline Phosphatase, A4312, Sigma Aldrich; 1:3000) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... the first incubation was performed with mouse anti-RFP and rabbit anti-PCP4(respectively: 1:500, Invitrogen, # 710530 and 1:300; Sigma-Aldrich, #HPA005792, RRID: AB_1855086). The secondary incubation was performed with anti-mouse conjugated to Alexa 488 and anti-rabbit IgG2a conjugated to Alexa 568 (respectively ...
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bioRxiv - Biochemistry 2021Quote: ... followed by TBST-Blotto containing two primary antibodies: monoclonal mouse anti-FLAG M2 (1:2,000 dilution, Sigma) and monoclonal rabbit anti-RNA polymerase beta (1:2,000 dilution ...
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bioRxiv - Cell Biology 2020Quote: The following commercial antibodies were used in this study: anti-β-actin (mouse; Sigma, A1978; RRID: AB_476692), anti-emerin (rabbit ...
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bioRxiv - Microbiology 2020Quote: ... Slides were subsequently stained for mesenchymal lineage cells using a mouse anti-vimentin (Sigma; Cat. No. HPA001762), at a 1:1000 dilution in antibody diluent overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with either HRP-linked goat anti-mouse IgG antibody (dilution 1:10,000; Sigma-Aldrich) or HRP-linked donkey anti-rabbit IgG antibody (dilution 1:10,000 ...
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bioRxiv - Cell Biology 2020Quote: ... We then incubated cells in serum free DMEM with 1/1000 mouse anti-Flag antibody (Sigma F1804) for 60 min at 37°C ...