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Citations for Millipore Sigma :
6501 - 6550 of 10000+ citations for Mouse Anti Rubella virus Glycoprotein E1 1715 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... incubated for 1 hr with a 1:10,000 dilution alkaline phosphatase conjugated anti-mouse secondary antibody (Sigma), prior to a final 5 x TBST washes and incubation with BCIP/NBT substrate ...
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bioRxiv - Biochemistry 2024Quote: ... The coverslips were incubated with primary antibody anti-FLAG®M2 (1:100, Sigma Aldrich, mouse F3165) or anti-FLAG (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... Normal Mouse IgG (12-371, Sigma-aldrich, Mouse polyclonal).
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bioRxiv - Cell Biology 2019Quote: ... (Piperno and Fuller 1985)), mouse-anti-β-tubulin (E7; at 1:100; Developmental Hybridoma Studies Bank, University of Iowa) and rabbit-anti-Zwf1 (Sigma A9521; at 1:10,000), followed by goat-anti-mouse-680 (LI-COR 926-68070 ...
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bioRxiv - Systems Biology 2021Quote: ... for 1.5 hours at room temperature before being stained for bait protein with mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody; Sigma-Aldrich, Cat# F3165; 1:500) in blocking buffer overnight at 4℃ ...
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bioRxiv - Genetics 2022Quote: ... the membrane was probed with the primary antibody overnight at 4°C (rabbit anti-beta galactosidase antibody, 15518-1-AP Protein Tech, dilution 1/1000: mouse anti-alpha-tubulin, T6199 Sigma-Aldrich, dilution 1/10 000) and then 1 hour at room temperature with the HRP linked secondary antibody (HRP-linked anti-rabbit IgG ...
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bioRxiv - Biochemistry 2021Quote: ... primary antibodies followed by anti-mouse (#A4416) and anti-rabbit (#A0545) secondary antibodies conjugated to horseradish peroxidase (both are from Millipore-Sigma and used at 1:10,000). WesternBright Sirius chemiluminescent horseradish peroxidase substrate (Advansta ...
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bioRxiv - Pathology 2023Quote: ... Western blot experiments were performed as previously described30 using mouse anti-pSMAD2 (cell signalling #3108S diluted 1/500) detected using anti-mouse IRDye 800CW (Licor #925-32211) and α-tubulin (Sigma #T6199 diluted 1/2000) detected using anti-mouse IRDye 680RD (Licor #925-68070) ...
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bioRxiv - Cancer Biology 2023Quote: The antibodies employed in this experiment were as follows: mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (G8795, 1:4000), anti-Flag (F3165, 1:2000) from Sigma-Aldrich (St. Louis, MO, USA); mouse anti-hemagglutinin (HA ...
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bioRxiv - Systems Biology 2023Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signalling Technology, Danvers, MA, USA; Goat Anti-Mouse – Sigma-Aldrich, St. Louis, MO, USA) for 1 hour at 24°C with agitation ...
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bioRxiv - Biochemistry 2023Quote: ... The blots were incubated with HRP-conjugated secondary antibodies (Pierce 31450 for rabbit anti-mouse IgG-HRP; Sigma A6154 for goat anti- rabbit IgG-HRP) before being visualization of protein signals using the ECL max kit (Bio-rad).
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bioRxiv - Genomics 2024Quote: ... rabbit anti-NDC80 (dilution 1:1000) (Oliveira et al. 2024) and mouse anti αtubulin (dilution 1:100, Sigma-Aldrich, St. Louis, MO; catalog number T6199). Antibodies against KNL1 and NDC80 were originally developed using respective peptides EDHFFGPVSPSFIRPGRLSDC and EQGINARDAERMKRELQALEG from Cuscuta sp ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated in primary antibody for 12-18 hours at RT (goat anti-ChAT, 1:500, Millipore Sigma and Cat#: AB144P; mouse anti-NeuN Clone A60-AlexFluor 488 conjugated, Millipore Sigma and Cat #: MAB377X). Slices were incubated in donkey anti-goat Alexa Fluor 555 (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... hCEcto stem cells were grown on collagen-coated six well-plate to 50% confluence before they were treated with virus solution and Polybrene (1μg/mL) (Sigma-Aldrich, # H9268) overnight at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... elephas RNAs were reverse transcribed with an oligo dT primer using an enhanced avian myeloblastosis virus reverse transcriptase enzyme (Sigma-Aldrich) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... 6×105 cells were infected in 6-well plates with 125uL of high titer virus in standard media with 5ug/mL Polybrene (Sigma-Aldrich). Media was changed after 24 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... The virus was harvested 48 hours post transfection and filtered through a 0.45 μm PES filter (Merck-Millipore, Burlington, Massachusetts, USA). PM-PDX derived cells were cultured in complete EMEM until a density of approximately 60% was reached ...
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bioRxiv - Cancer Biology 2020Quote: ... Infection of P3 and T98G cells was performed by centrifugation of cells with virus at 2225 rpm for 90 minutes at room temperature in the presence of 10 μg/mL polybrene (Sigma-Aldrich). Enrichment selection using puromycin (1 μg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... Infection of P3 and T98G cells was performed by centrifugation of cells with virus at 2225 rpm for 90 minutes at room temperature in the presence of 10 μg/mL polybrene (Sigma-Aldrich). Enrichment selection using puromycin (1 μg/mL ...
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bioRxiv - Biochemistry 2021Quote: ... Virus particle-containing cell culture supernatants were sterile filtered over 0.22 µM PES syringe filters (Millex-GP, Sigma Millipore, Burlington, MA), mixed 1:1 with fresh growth medium ...
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bioRxiv - Biochemistry 2021Quote: ... Virus particle-containing cell culture supernatants were sterile filtered over 0.22 µM PES syringe filters (Millex-GP, Sigma Millipore, Burlington, MA), mixed 1:1 with fresh growth medium ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 24-48 h with the virus supernatant supplemented with 8 µg ml-1 polybrene (Sigma-Aldrich #H9268) before being subjected to further experimental procedures.
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bioRxiv - Microbiology 2020Quote: ... Duplicate cDNA preparations from each sample were generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... all transductions were performed in multiple plates with a range of virus volumes and 8 μg mL−1 polybrene (EMD Millipore), but only the pool with the most optimal transduction efficiency was expanded and/or screened.
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bioRxiv - Microbiology 2021Quote: 3 μl of A/PR/8/34 (H1N1) influenza virus mixed with 1 μl of 5x BSA-coated 10 nm colloidal gold (Sigma-Aldrich) were applied to freshly washed and glow-discharged R2/2 holey carbon copper grids (Quantifoil) ...
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bioRxiv - Microbiology 2021Quote: A549-ACE2 cells were infected by incubating the virus for 1h with the cells maintained in DMEM supplemented with 1 μg/ml TPCK-trypsin (Sigma #4370285). The viral input was then removed and cells were kept in DMEM supplemented with 2% FBS ...
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bioRxiv - Microbiology 2021Quote: Duplicate cDNA preparations from each sample were generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma−Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... whole-bodies of virus-injected females (DENV and ZIKV-infected) were homogenised with glass beads in Trizol (Sigma-Aldrich, MA, USA) on a Precellys 24 homogeniser (VWR).
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bioRxiv - Cancer Biology 2020Quote: ... Next day cells were incubated for 24 h with the virus-containing supernatant diluted in culture medium and 3 µg/mL Hexadimethrine bromide (polybrene, Sigma Aldrich). Afterwards medium was changed and after additional 24 h the medium was changed to selection medium containing 1 µg/mL puromycin (Puromycin dihydrochloride ...
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bioRxiv - Cancer Biology 2020Quote: ... Next day cells were incubated for 24 h with the virus-containing supernatant diluted in culture medium and 3 µg/mL Hexadimethrine bromide (polybrene, Sigma Aldrich). Afterwards medium was changed and after additional 24 h the medium was changed to selection medium containing 1 µg/mL puromycin ...
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bioRxiv - Cancer Biology 2022Quote: ... P12-ICHIKAWA cells were infected with Lenti-FUW-Luc-mCh-puro virus and selected in liquid culture with puromycin (Sigma-Aldrich) 2.5µg/mL for 7 days following mCherry+ cell sorting.
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bioRxiv - Microbiology 2019Quote: ... Duplicate cDNA preparations from each sample were generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... 1.8 μg of pRSV-Rev and 1.5 μg of plasmid containing pAIP-NSP2-mCherry or pAIP-NSP5 and the human immunodeficiency virus long terminal repeats were co-transfected with Lipofectamine 3000 (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... virus like particles were incubated with 20 μL of 10% SDS and 2 μL of proteinase K (Sigma, 20 mg/mL) for 20 min at 56 °C ...
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bioRxiv - Cell Biology 2019Quote: ... were added to each well (around 6 × 105 cells per well) along with a no virus control with 8 µg/mL polybrene (Sigma-Aldrich). Cells were incubated overnight (12-16 hours) ...
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bioRxiv - Synthetic Biology 2021Quote: ... viral transductions were performed overnight in a 6-well dish using 800 µl of standard ESC medium with 200 µl virus-containing supernatant supplemented with 8 µg/ml polybrene (TR-1003; Sigma-Aldrich). The selection of positive clones was performed by supplementing the culture medium with 1µg/ml puromycin for 3 days ...
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bioRxiv - Microbiology 2020Quote: ... RNA was converted to cDNA using the First Strand cDNA synthesis kit for reverse transcription (avian myeloblastosis virus [AMV]; Millipore-Sigma). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Microbiology 2021Quote: ... virus like particles were incubated with 20 μL of 10% SDS and 2 μL of proteinase K (Sigma, 20 mg/mL) for 20 min at 56°C ...
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bioRxiv - Genomics 2021Quote: The titer of lentivirus was determined by transducing 500,000 cells with varying amount (0, 0.5, 1.0, 2.0, 4.0 and 8.0 µL) of concentrated virus and polybrene (Millipore, TR-1003-G, 8 µg/mL,). Viral transduction was performed by centrifuging the lentivirus and cell combination at 1000 RCF for 90 min at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... and 500 μL) were added to each well along with a no virus control with 7 μg/mL polybrene (Sigma-Aldrich). Cells were incubated overnight ...
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bioRxiv - Immunology 2021Quote: ... On the following day the virus-containing medium was collected and filtered using 0.45 µm filter (Millipore Sigma, Cat. no: SLHVM33RS). Fresh HEK293T cells were infected by adding the lentivirus-containing medium to culture medium followed by 1 to 2 day incubation ...
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bioRxiv - Immunology 2020Quote: ... The swabs were placed into 1 ml of Trizol or 2 ml of virus transport medium comprising tissue culture medium 199 (Sigma-Aldrich) supplemented with 25mM HEPES ...
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bioRxiv - Microbiology 2022Quote: ... A 500 μL aliquot was collected every 10−50 s (depending on the virus) and mixed with 5 μL of 5,000 mg L−1 sodium thiosulfate (Sigma-Aldrich, Germany) to instantly quench the residual free chlorine ...
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bioRxiv - Microbiology 2022Quote: KSHV BAC16 virus stock was prepared by inducing lytic cycle in iSLK-BAC16 cells with Doxycycline (1 µg/ml; Sigma-Aldrich) and sodium butyrate (1 mM ...
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bioRxiv - Neuroscience 2022Quote: ... RabiesΔG-mCherry virus (titer of around 110 TU/ml) was mixed at a 2:1 ratio with silk fibroin (Sigma #5154) to make rabies injection solution (Jackman et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... The medium containing the virus was collected 48 hours post-transfection and filtered through a 0.45 µm polyethersulfone (PES) filter (Merck Millipore, Billerica, MA).
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bioRxiv - Microbiology 2022Quote: ... the cells were washed three times with PBS and virus media with 1 μg/ml TPCK treated trypsin (Sigma-Aldrich, USA) was added ...
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bioRxiv - Cell Biology 2024Quote: ... virus was collected and used to infect NIH3T3 cells with 6 µg/ml polybrene (Sigma, St Louis, MO, USA; #TR-1003) for another 12 h ...
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bioRxiv - Biochemistry 2024Quote: ... The HeLa cells were transduced in 6-well plates with 1 mL of unconcentrated virus + 10 µg/mL polybrene (TR-1003-G, Sigma Millipore) per well for 24 h ...
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bioRxiv - Immunology 2024Quote: ... dissociated organoid cells were seeded in virus-containing organoid culture media with 5 μg/ml polybrene (EMD-Millipore TR-1003-G) overnight ...