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Citations for Millipore Sigma :
6101 - 6150 of 10000+ citations for Recombinant Human ROR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Gels were stained with ReadyBlue Protein Gel Stain from Sigma-Aldrich (USA) and imaged and analyzed on a ChemiDoc Imaging System by Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2023Quote: Total protein was extracted from cell lines using TRI Reagent (Sigma, UK). 40μg of isolated protein samples were then separated using 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Physiology 2023Quote: ... Final protein concentrations were calculated by Bradford assay (Sigma-Aldrich Canada, Ltd.,) according to manufacturer’s guidelines with bovine serum albumin (BioRad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... total protein was detected with Amido Black (Sigma-Aldrich, St. Louis, MO). Chemiluminescence was measured in a ChemiDoc Imaging System (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... Proteins were visualized using peroxidase-coupled secondary antibodies (rabbit-POD, Sigma-Aldrich, A6154 ...
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bioRxiv - Cancer Biology 2023Quote: ... for 2 h on ice before mixing with protein G-Sepharose (Millipore). The antibody complex was washed three times in lysis buffer and once in Tris-buffered saline (TBS) ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µl Magna ChIP™ Protein A Magnetic Beads (Sigma, 16-661) and antibodies (1 µg per 1 million-cell chromatin ...
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bioRxiv - Genetics 2023Quote: ... Protein lysate was prepared in Radio-immunoprecipitation assay (RIPA) buffer (Merck Millipore) with a protease inhibitor cocktail (Roche ...
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bioRxiv - Bioengineering 2024Quote: ... Protein expression was then induced by addition of 1M IPTG (Sigma-Aldrich), before harvesting of cells after 8 hours ...
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bioRxiv - Cell Biology 2023Quote: ... and protein analysis of tau and β-III-tubulin (Sigma T-8660) by western blot as described above.
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bioRxiv - Biochemistry 2023Quote: ... Proteins were then transferred to 0.45 µm PVDF membranes (Immobilon-P, Millipore) in a wet tank apparatus ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were transferred to polyvinylidene fluoride (PVDF) membrane (Millipore, Cat# ISEQ00010), and blocked with blocking buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Protein was labeled using an atto-488-NHS ester (Sigma Aldrich 41698) and then dialyzed again to remove unconjugated dye ...
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bioRxiv - Immunology 2023Quote: ... proteins (50 μM) and TCEP (5 mM) reducing agent (Sigma Aldrich, USA) were mixed 1 hour at RT and then left overnight (O/N ...
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bioRxiv - Microbiology 2024Quote: ... The cleaved protein was concentrated using Amicon 10 KDa MWCO concentrators (Millipore), and further purified by size exclusion chromatography using a Superdex 200 (16/60 ...
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bioRxiv - Microbiology 2024Quote: ... the separated proteins were transferred onto Immobilon-P PVDF membrane (Merck Millipore). The membrane was blocked with 10% skim milk in Tris Buffered Saline ...
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bioRxiv - Immunology 2023Quote: Dry protein pellets were dissolved in 100mM TrisHCL (pH 8.5, Sigma T1503) to determine the protein concentration using the Bradford protein assay (Thermo Scientific 23200) ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µg protein per sample was reduced with 3 mM TCEP (Sigma) and alkylated with 10 mM iodoacetamide (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was concentrated using Amicon Ultra-4 10 kDa MWCO (Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were electrotransferred to Immobilon-FL polyvinylidene difluoride (PVDF) membranes (Merck Millipore) and blocked with Intercept Blocking Buffer (LI-COR Biosciences ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... 50 µL of agarose beads coupled to protein A (Millipore 16–157) or G (Millipore 16–201 ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were detected using anti-FLAG (Sigma-Aldrich, ST. Louis, MO, USA) and HRP-labeled anti-mouse IgG (Cytiva ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred to PVDF membranes (Millipore). The membranes were then blocked and probed with anti-Drp1 (1:1000 ...
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bioRxiv - Genetics 2024Quote: ... Protein was transferred to a methanol-treated polyvinylidene difluoride membrane (Millipore Sigma) using a Mini Trans Blot Electrophoretic Transfer Cell (Biorad ...
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bioRxiv - Pathology 2024Quote: ... Proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Immobilon-P, Merck Millipore), blocked with 5% non-fat dry milk ...
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bioRxiv - Cell Biology 2024Quote: ... Exogenous protein expression was induced 24h after transfection by adding Doxycycline (Sigma) to the media at a concentration of 50 ng/ml and induction was performed for 24h ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Proteins in the SDS gels were transferred to PVDF (Cat# IPVH00010, Millipore) membrane using an electroblotting apparatus (Bio-Rad ...
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bioRxiv - Biophysics 2024Quote: ... The digested protein samples were then concentrated with an Amicon Ultra (Millipore) concentrator (50 kDa MWCO ...
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bioRxiv - Cancer Biology 2024Quote: ... the protein was transferred to 0.45μm PVDF membranes (Millipore Sigma, Cat#IPVH00010) with transfer buffer (formula ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins were transferred onto a 0.45-μm polyvinylidene difluoride membrane (Millipore). Next ...
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bioRxiv - Cell Biology 2024Quote: Cellular protein was collected in RIPA buffer (R0278, Millipore Sigma, Burlington, MA) containing ReadyShield® Protease and Phosphatase Inhibitor Cocktail (PPC- 2020 ...
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bioRxiv - Cell Biology 2024Quote: ... Total protein bands were reversibly stained with Fast Green FCF (Sigma, F7252) and imaged on ImageQuant LAS 4000 or Amersham ImageQuant 800 (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... followed by the addition of 5 mg protein A beads (Sigma, P1406) in PBS and incubated at 4°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... was dissolved into 5 mL of BugBuster™ protein extraction reagent (Sigma). Using a sterile loop ...
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bioRxiv - Cell Biology 2024Quote: ... Ice-cold protein extraction lysis buffer was composed of RIPA (Sigma, R0278), Protease inhibitor cocktail (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration in each sample was measured using BCA assay (Sigma-Aldrich). 30µg of each sample were then prepared using the iST-NHS kit (Preomics) ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted protein was concentrated using Amicon Ultra 10K spin columns (Sigma-Aldrich) and dialyzed against hSSB storage buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Bioengineering 2024Quote: ... the protein was concentrated using Amicon centrifugal filters (Millipore, Burlington, MA, USA) with a molecular weight cut off of 10 kDa.
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were transferred to polyvinylidene difluoride (PVDF) membranes (Immobilon-P, Millipore, IPVH100010). Membranes were blocked for at least 1 h with 5% non-fat dry milk and 3% BSA in TBS-T (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated by SDS-PAGE and transferred to PVDF membranes (Millipore). The membranes were blocked with 5 % skimmed milk in TTBS buffer (10 mM Tris-HCl ...
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bioRxiv - Plant Biology 2024Quote: ... MBP fusion proteins were expressed in Escherichia coli Rosetta (DE3) pLysS (Novagen) and purified using Amylose Resin (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... The separated proteins were then transferred to a PVDF membrane (Millipore, IPVH00010) and immunodetection was carried out using antibodies against the target proteins ...
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bioRxiv - Biochemistry 2024Quote: ... and proteins were concentrated using an Amicon ® Ultra Centrifugal Filter (Millipore) with a 30 kDa cutoff ...
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bioRxiv - Biochemistry 2024Quote: ... Protein-containing factions were concentrated with Amicon Ultra Filter Devices (Millipore, USA) 10 kDa cutoff ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined using a BCA assay kit (#QPBCA, Sigma-Aldrich). Protein samples were mixed with DTT (#D9779-10G ...
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bioRxiv - Immunology 2024Quote: ... were coupled to 30 μl Protein G Sepharose™ beads (#P3296, Millipore) by 2 h incubation at 4°C with rotation ...
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bioRxiv - Microbiology 2024Quote: ... Proteins from gels were transferred on to polyvinylidene difluoride membrane (Millipore, #IPVH00010) at 30 V overnight ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations in the collected fractions were measured using Bradford reagent (Sigma).
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bioRxiv - Biophysics 2021Quote: ... Micropatterned coverslips were functionalized with a solution of 10 µg mL-1 FN from human plasma (Sigma Aldrich #F1056) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... solutions of 89Zr-labeled TDM1 (4 μCi/μg) were prepared in PBS (pH 7.5) containing 1% w/v human serum albumin (HSA, Sigma) and 0.1% w/v sodium azide (NaN3 ...