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Citations for Millipore Sigma :
5951 - 6000 of 10000+ citations for Mouse Anti Rubella virus Glycoprotein E1 1715 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... virus was mixed with 0.36 million cells in growth media supplemented with 8 μg/mL Polybrene (Sigma-Aldrich, H9268) and incubated overnight ...
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bioRxiv - Immunology 2021Quote: ... The virus particles in the supernatant were concentrated to 100μl using Amicon® Ultra-15 Centrifugal Filters (Sigma-Aldrich Chemicals Private Limited ...
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bioRxiv - Immunology 2021Quote: ... The supernatant containing pseudo-virus was centrifuged at 3000g and filtered through a 0.45μm sterilized membrane (Millipore, Burlington, MA). The titer of virus generated by engineered Zhou-COVID-19-Spike plasmid is ten-fold higher than non-engineered Spike protein sequence (data not shown) ...
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bioRxiv - Microbiology 2021Quote: ... cell monolayers were washed twice with PBS before adding virus inoculum in DMEM supplemented with 1μg/ml of trypsin TPCK (Sigma) and no FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... HUVECs were transduced with optimal volume of lentiviral virus at 50% confluence in MV2 medium and 8μg/ml Polybrene (Sigma). After 24 h ...
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bioRxiv - Biochemistry 2022Quote: The virus was precipitated from the cell supernatant by adding 8 % (w/v) polyethylene glycol (PEG) 8000 (Sigma-Aldrich), incubating at +4 °C with gentle mixing for 3 h ...
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bioRxiv - Systems Biology 2023Quote: ... HEK293 cells were then transduced overnight with the filtered virus in the presence of 8 µg/mL polybrene (Millipore); the amount of virus used was optimized to ensure the infection rate of ∼20% ...
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bioRxiv - Microbiology 2023Quote: ... The medium for the culture of influenza A virus in MDCK cells (virus medium) was prepared by supplementing Minimal Essential Medium with 4.3% bovine serum albumin (BSA; Sigma) and Normocin.
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bioRxiv - Microbiology 2024Quote: ... The virus particles were further purified by layering them over a discontinuous 5% to 50% Nycodenz gradient (Sigma-Aldrich) in TNE buffer ...
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bioRxiv - Microbiology 2024Quote: All transductions were performed via spin inoculation at 1050 x g for 2 hr at 25°C with equal virus amounts determined by Gag p24 mass in medium containing 4µg/mL polybrene (Sigma). MDMs were inoculated with 10µg p24 mass equivalents of NL4-3 ΔGPE-GFP virus or 20µg p24 mass equivalents of 3xFLAG-89.6-Vpr ...
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bioRxiv - Physiology 2024Quote: ... C2C12 mouse myoblasts were seeded 24h prior to infection and then transduced with virus-containing supernatant supplemented with 8μg/mL polybrene (Millipore) as shown previously40 ...
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bioRxiv - Genetics 2023Quote: ... RPE-1 cells were transduced with lentivirus at an MOI of 0.3-0.5 (usually between 50-100 µL of virus/well) and 10 µg/mL Polybrene (Sigma-Aldrich). After 48 hours ...
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bioRxiv - Immunology 2023Quote: ... Next the pseudotyped virus-containing culture medium was diluted 50% in culture medium supplemented with 8μgml−1 polybrene (Sigma) and immediately applied onto MutuDC1 cells ...
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bioRxiv - Microbiology 2023Quote: ... Virus supernatant was removed 6 hours post transduction and cells were maintained for 48 hours before puromycin (Sigma-Aldrich) selection ...
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bioRxiv - Microbiology 2023Quote: ... Confluent MDCK cells were incubated with each serial dilution for 1h at 37°C then input virus was removed by aspiration and cells overlaid with SFM containing 0.14% BSA (Sigma), 0.8% Avicel© (FMC BioPolymer ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transduced with the respective virus particles in an equal ratio using 5 µg/mL polybrene (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... concentrated virus particles were freshly thawed and diluted in phosphate-buffered saline (PBS, Merck, 806552, Sigma-Aldrich, Darmstadt, Germany) based on the p24 antigen concentration in the supernatant ...
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bioRxiv - Biochemistry 2023Quote: ... Virus-containing supernatants were harvested after 48 h transfection and filtered by syringe through a 0.45 μM filter (Millipore) to eliminate cell contaminates ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were transduced with the virus for 48 h in the presence of 4 µg/ml polybrene (Sigma H9268).
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bioRxiv - Microbiology 2023Quote: ... neutralized whole-virus samples were incubated in D2O buffer (99% deuterium oxide, Sigma 151882, supplemented with 120 mM NaCl). 40 μL D2O buffer was added to every 10 μL of neutralized virus (final D2O concentration 78% ...
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bioRxiv - Cancer Biology 2023Quote: ... subconfluent cultures were incubated with virus-containing supernatants in the presence of 4µg/ml polybrene (Sigma-Aldrich , TR-1003) for 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... virus-containing supernatants were used to transduce A2EN cells in the presence of 8 µg/ml polybrene (Sigma-Aldrich). Cells were co-transduced with lentiviral vectors encoding the two distinct sgRNAs that target the same gene ...
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bioRxiv - Cancer Biology 2024Quote: ... Virus transduction of target sarcoma cells was performed in the presence of 8.0 μg/mL polybrene (Sigma-Aldrich, #H9268) and puromycin selection (3 μg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... 1×107 OE19 cells were treated with virus (MOI 0.7) and 5 μg/mL Polybrene (EMD Millipore, TR-1003). Polyclonal cells were selected for 2 weeks in 500 ng/μL puromycin (Sigma ...
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bioRxiv - Systems Biology 2024Quote: ... the virus containing supernatant was centrifuged 500 x g for 5min and filtered by using 0.45um PES filters (Millipore). The lentivirus was concentrated by using Lenti-X concentrator (Clontec) ...
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bioRxiv - Genetics 2021Quote: ... Brains were then incubated with secondary antibody (goat anti-mouse Alexa 488 or 555, or goat anti-rabbit Alexa 488 or 555, Sigma Aldrich, 1:500) and mounted with Antifade ...
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bioRxiv - Neuroscience 2021Quote: ... then blocked with 5% normal goat serum in 0.5% PBS-T for 2 hr at RT and stained with pre-absorbed rabbit anti-PER (1:1000) 50 and mouse anti-GFP (Sigma G6519, 1:200) in 5% goat serum in 0.5% PBST for at least 48 hr at 4°C ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... The following primary antibodies were applied for 24 h (or 48 h for anti-cFos antibodies) at 4°C: mouse anti-APC/CC1 Ab-7 (Millipore, OP80, 1:100), chicken anti-mCherry (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies were the following: rabbit polyclonal anti-ERGIC-53/p58 and mouse anti-MAP2A purchased from Sigma-Aldrich (St. Louis, MO, USA) (code ...
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bioRxiv - Neuroscience 2020Quote: ... at 1:500 and anti-vasopressin (PS 45, a generous gift from Hal Grainger) or mouse anti-tyrosine hydroxylase (EMD Millipore; cat# MAB318) at 1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies and dilutions used in this study were: mouse anti-PV (1/4000; Swant, Marly, Switzerland) and rabbit anti-GABA (1/200; Sigma-Aldrich, Dorset, UK). Upon completion of the primary incubation step ...
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bioRxiv - Microbiology 2021Quote: ... followed by horseradish peroxidase (HRP)-conjugated anti-mouse antibodies (Jackson, 715-035-150, 1:10000) or anti ß-actin-HRP (A3854, Sigma, 1:100000) antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were centrifuged at 1000 × g for 1 minute and set up in new tubes with either anti-Mouse IgG1 control or EZview Red Anti-Flag Affinity Gel (Sigma, St. Louis, MO) (to immunoprecipitate Flag epitope-tagged proteins ...
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bioRxiv - Physiology 2022Quote: ... and with peroxidase- conjugated secondary antibodies (Anti-mouse IgG, HRP conjugate, EMD Millipore, AP160P; Anti-rabbit IgG, HRP-linked, EMD Millipore, 12-348) for 1 h at room temperature ...
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β-catenin signaling via astrocyte-encoded TCF7L2 regulates neuronal excitability and social behaviorbioRxiv - Neuroscience 2020Quote: ... the membranes were incubated with secondary antibodies (anti-Rabbit IgG – peroxidase antibody, Sigma Aldrich, A0545; anti-Mouse IgG – peroxidase antibody, Sigma Aldrich, A9044) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers, MA, USA; Goat Anti-Mouse – Sigma-Aldrich, St ...
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bioRxiv - Microbiology 2022Quote: ... for 1 h and incubated with the rabbit monoclonal anti-HA antibody (CST, 3724, 1:500) or mouse monoclonal anti-Flag antibody (Sigma, F3165, 1:100) overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: Antibodies used in immunofluorescence and Western blot include: anti-MMP9 rabbit polyclonal antibody (# AB13458) and anti-GAPDH mouse monoclonal antibody (# CB1001) purchased from EMD Millipore (Billerica, MA); anti-Src rabbit antibody (# 2108) ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies used were rabbit anti-tyrosine hydroxylase (Pel-Freez, P40101, 1:1000) and mouse anti-beta III tubulin (Sigma, T8660, 1:2000).
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers, MA, USA; Goat Anti-Mouse – Sigma-Aldrich, St ...
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bioRxiv - Physiology 2021Quote: ... PLA was performed using the Duolink® In Situ Detection Reagents Red with Duolink® In Situ PLA probe anti-rabbit PLUS and anti-mouse MINUS (Sigma-Aldrich). The nuclei of cells were stained using Duolink® In Situ Mounting Medium with DAPI (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... incubated in mouse anti-Pax7 antibody (undiluted, Antibody Registry ID: AB_528428) and rabbit anti-laminin (1:250, #L9393,Sigma-Aldrich, St. Louis, MO) overnight at 4°C ...
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bioRxiv - Zoology 2021Quote: ... in PBS and then incubated in the primary antibodies (Rabbit Anti-phospho-histone H3, Sigma-Aldrich H0412 and Mouse Anti-acetylated-α-tubulin, Sigma-Aldrich T6793 ...
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bioRxiv - Microbiology 2022Quote: ... cells were incubated overnight at 4 °C with 1:500 rabbit anti-ACP antibody (Gallagher & Prigge, 2010) and 1:500 mouse anti-FLAG antibody (Catalogue no. F3165, Millipore Sigma, MO, USA). The cells were washed with PBS three times and then incubated for 2 h with 1:1000 goat anti-mouse Alexa 488 (Catalogue no ...
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bioRxiv - Plant Biology 2024Quote: ... Membranes were probed with either rabbit anti-RACK1A (1:500) (Chang et al., 2005) or mouse anti-α-Tubulin (1:3000) (Sigma-Aldrich T6074) antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated with GFAP antibody (mouse anti-GFAP; 3670S; 1:1000; CST, Danvers, MA) and TH antibody (sheep anti-TH; 1:1000; AB1542; Millipore Sigma, Burlington, MA) together at 4°C for 18 hours followed by appropriate secondary antibodies (donkey anti-mouse IgG [H+L] ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated for 72-hours in the same blocking solution with rabbit anti-CRF (Peninsula,T-4037, 1:1K) and mouse anti-TH (Millipore, MAB318, 1:5K) at 4°C on a shaker followed by 24-hours at room temperature (RT) ...
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bioRxiv - Microbiology 2023Quote: ... overnight at 4 °C following incubation with mouse anti-rAgApyrase antibody (1:10,000 in blocking buffer) or rabbit anti-tPA antibodies (1:2500) (Millipore-Sigma, Marlborough, MA, USA). Membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase (1:2500 in blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were incubated with primary antibodies prepared in 1% skim milk and 1% Bovine Serum Albumin in TBST for 2 hours (mouse anti-SERT 1:7000, MAb Technologies, ST51-2; rabbit anti-Tph2 1:1000, Abcam, ab184505, RRID: AB_2892828; mouse anti-βActin 1:5000, Sigma, A1978, RRID: AB_476692). After washing with TBST ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding to N protein was detected using conventional two-step ELISA with an HRPO-conjugated anti-mouse or anti-rat Fc antibody (Sigma Aldrich cat # A9309). All assays included a standard curve of mouse neutralising S1 antibody and mouse anti-N antibody (Sino Biological) ...