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Citations for Millipore Sigma :
5901 - 5950 of 10000+ citations for Mouse Anti Rubella virus Glycoprotein E1 1715 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... followed by 1 h incubation at RT in secondary antibodies (Anti-Rabbit: A0545; Anti-Mouse: A6782, Sigma-Aldrich Quimica S.A., Madrid, Spain) diluted 1:10,000 in blocking buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue sections incubated at room temperature overnight in primary antibody medium or control medium (1:100 primary anti β-catenin antibody from rabbit Sigma C2206 + 1:100 primary anti β-actin antibody from mouse Sigma A5441 + PBS pre-incubation medium ...
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bioRxiv - Systems Biology 2020Quote: ... rabbit anti-PDGFRα (1:400, gift from Prof Stallcup); goat anti-PDGFRα (1:200, R&D Systems; mouse anti-APC (CC1; 1:300, Millipore); rabbit anti-GFAP (1:300 ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated overnight at 4°C with primary antibodies (anti-β III tubulin mouse monoclonal, Sigma, T7816 and anti-DCX, Guinea Pig polyclonal, Millipore, AB5910) at dilution of 1:1000 in 1% BSA in PBST (0.5% triton added to PBS) ...
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bioRxiv - Cell Biology 2022Quote: ... the membranes were incubated with HRP-conjugated secondary antibody (1:10,000; anti-rabbit, Sigma-Aldrich A0545 or anti-mouse, Sigma-Aldrich A0168) in TBST with 1% milk ...
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bioRxiv - Cancer Biology 2022Quote: ... filters were incubated for 1 hour at RT with either goat-anti rabbit (1:5000) or goat-anti mouse (1:2000) horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck Millipore, Darmstadt, Germany). Blots were developed using SuperSignal West Pico Chemiluminescent Substrate or Femto Maximum Sensitivity (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... of anti-S murine antibodies (including cross-reactive mAbs to SARS-CoV) and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were fixed with 4% PFA and immunostained with mouse anti-HA (Covance, 1:1000) or rabbit anti-Flag (Sigma, 1:1000), followed by incubation with AlexFluor-647-conjugated anti-human Fc γ and species-specific AlexaFluor-488- or -546-conjugated secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 1 h with the proper secondary antibodies (1:400, goat anti-rat Alexa-Fluor® 568, ab175476, abcam; 1:400, goat anti-mouse Alexa-Fluor® 488, AP124JA4, Merck Millipore). For nuclear counterstaining slices were incubated with DAPI (1:1000 ...
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bioRxiv - Systems Biology 2020Quote: ... Blots were incubated with appropriate secondary antibodies (Anti-Rabbit IgG-Peroxidase Sigma-Aldrich A0545 and Anti-Mouse IgG-Peroxidase Sigma-Aldrich A9044) and imaged using GE Amersham Imager 600.
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bioRxiv - Plant Biology 2021Quote: ... The glutathionylated VirE2 was detected by the anti-glutathione monoclonal primary antibody (My biosource, 1:1000) and anti-mouse (Sigma, 1:1000) secondary antibody (Klaus et al ...
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bioRxiv - Cell Biology 2020Quote: ... Duolink® In Situ PLA Probe Anti-Rabbit MINUS and Anti-Mouse PLUS and Duolink® In Situ Detection Reagents (Sigma-Aldrich) were used ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein detection was achieved using the following antibodies: anti-Toddler (rabbit, 1:500, provided by the Reversade Lab11) and anti-tubulin (mouse, 1:10000, Sigma-Aldrich T6074).
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bioRxiv - Genetics 2020Quote: ... using 1:1000 rabbit anti-Bestrophin1 antibody (LAgen Laboratories; Cat# 016-Best1-01) and 1:1000 mouse anti-Actin antibody (Millipore; Cat# MAB1501) as primary antibodies.
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated in PBS-T-milk for 1 h using 1:10000 dilutions of anti-rabbit or anti-mouse HRP-linked secondary antibodies (A0545 or A9044 Sigma antibodies, respectively) as required ...
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bioRxiv - Neuroscience 2022Quote: ... Then membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase conjugated secondary antibodies (1:3000, Sigma Aldrich, St. Louis, MO) for 1 hr ...
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bioRxiv - Neuroscience 2022Quote: ... The following primary antibodies were used for immunostaining: monoclonal mouse anti-mutant huntingtin (1/3000; PHP2(34)) and polyclonal guinea pig anti-NEUN (1/1000; Millipore, No. ABN90P). All staining was performed with floating sections ...
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bioRxiv - Molecular Biology 2024Quote: ... Yhb1-TAP and α-tubulin proteins were detected using the polyclonal anti-mouse IgG antibody (ICN Biomedicals) and monoclonal anti-α-tubulin antibody (clone B-5-1-2; Sigma-Aldrich), respectively ...
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bioRxiv - Genetics 2024Quote: ... and secondary antibodies (anti-mouse HRP [1:2,000; Thermo Fisher Scientific] for TARS1 and anti-rabbit HRP [1:5,000; EMD Millipore] for actin) were applied in 2% milk solution ...
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bioRxiv - Cell Biology 2023Quote: ... sections were stained for ciliated cells (rabbit anti-RFX3, 1:100, Cat# HPA035689,Sigma-Aldrich, and mouse anti-AceTUB, 1:100, Cat# T6793, Sigma-Aldrich), club cells (rabbit anti-SCGB1A1 ...
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bioRxiv - Microbiology 2023Quote: ... Horseradish peroxidase-conjugated secondary antibodies (anti-mouse or anti-rabbit, at dilution 1:10000) and Immobilon® Western Chemiluminescent HRP substrate (Merk Millipore, Sigma Aldrich) were used to visualize FLAG-FlaA/FlaB and untagged FlaA/FlaB.
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bioRxiv - Microbiology 2023Quote: ... overnight at 4 °C following incubation with mouse anti-rAgApyrase antibody (1:10,000 in blocking buffer) or rabbit anti-tPA antibodies (1:2500) (Millipore-Sigma, Marlborough, MA, USA). Membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase (1:2500 in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... the first incubation was performed with mouse IgG2a anti-RGS14 (1:50, UC Davis/NIH NeuroMab Facility, #73-170) and rabbit anti-PCP4 (1:400, Sigma-Aldrich; #HPA005792). The secondary incubation was performed with anti-mouse IgG2a or anti-rabbit conjugated to Alexa 488 (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-VMAT2 (1:2000, gift of Dr. Gary Miller, Colombia University) and mouse anti-MAP2 (1:2000, Millipore Cat# MAB3418, RRID:AB_94856). These were subsequently detected using goat anti mouse or rabbit Alexa Fluor-488-conjugated (A-11001 RRID:AB_2534069 ...
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bioRxiv - Immunology 2023Quote: ... Sections were incubated for 48-72 hours with the following primary antibodies at 1:400: rabbit anti-Iba1 (Wako, 019-19741) and mouse anti-PSD-95 (Sigma-Aldrich, MAB1598). Sections were then washed 3x with PBS containing 0.05% Tween 20 and incubated with Alexa Fluorophore conjugated goat anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were incubated with secondary antibodies conjugated with PLA probes MINUS and PLUS: the PLA probe anti-mouse PLUS and anti-rabbit MINUS (Duolink®, Sigma-Aldrich). The incubation with all antibodies was accomplished in a humidified chamber for 1 h at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... Antibodies used in Western blot were (listed in Appendix Table S4): mouse monoclonal antibodies anti-β-actin and anti-UVRAG (both from Sigma-Aldrich), anti-LC3B (NanoTools) ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies used for immunofluorescence were: anti- cleaved Caspase-3 (Asp175) (1:400, rabbit, CST, #9661) and anti-FLAG (1:400, mouse, Sigma-Aldrich, #F3165) for detection of overexpressing proteins ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... Target cells were then transduced overnight with the filtered virus in the presence of 8 μg/mL polybrene (Millipore).
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bioRxiv - Genetics 2021Quote: ... The medium was replaced 24 h later and the virus particles were collected and filtered through 0.45 μm filters (Millipore), 24 or 48 h later.
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bioRxiv - Microbiology 2021Quote: ... freeze-thaw cycles) was followed by concentration of virus suspensions (i.e., filtered lysates) using 30kDA MWCO spin concentrator (Sigma-Millipore). From the retentate ...
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bioRxiv - Cell Biology 2022Quote: ... 293T or 293GP virus producing cells were transfected using the GeneJuice reagent according to the manufacturer’s recommendations (Merck Millipore). Two days after transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... After 2h of virus adsorption in the presence of 0.1 to 0.2 mg/ml of porcine pancreatin (Sigma-Aldrich) prepared in CMGF(- ...
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bioRxiv - Microbiology 2021Quote: ... Virus-containing flocs were then recovered from the sample by filtration onto 90mm 0.2um polycarbonate filters (Isopore, GTTP09030, Millipore) under gentle vacuum ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed and inoculated with virus diluted in infection media (DMEM with 0.3% BSA and 1μg/ml TPCK-treated trypsin (Sigma Aldrich)) and allowed to adsorb for one hour ...
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bioRxiv - Plant Biology 2019Quote: ... and 1 mM DTT) for 2 hours at 30°C with native Tobacco Etch Virus (TEV) protease (Sigma-Aldrich). The purified recombinant proteins were concentrated using Amicon centrifugal filters following the manufacturer recommendations (Millipore) ...
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bioRxiv - Microbiology 2019Quote: ... A 2 mL volume of warm virus medium (1X MEM with 100U/100 μg/mL penicillin/streptomycin, 0.3% bovine serum albumin [Sigma]) was added to each well ...
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bioRxiv - Microbiology 2019Quote: ... cDNA was generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: RNA was extracted from CsCl purified reovius preparations (~1×1010 virus particles) using TRI Reagent LS (T3934, Millipore Sigma) as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Each cell line was infected with varying volumes of concentrated virus in the presence of polybrene (Sigma Aldrich 107689) to determine the concentration that conferred survival in puromycin to 30% of cells ...
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bioRxiv - Cell Biology 2021Quote: ... The target cell lines at 50-60% confluency were then infected using 3 mL virus with polybrene reagent (Sigma). After 24 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... except that each virus was added to SVOG3e/rtTA cells and selected with 0.5 μg/ml Puromycin (Sigma P8833) and 0.6 mg/ml Geneticin to create polyclonal stable cell lines SVOG3e/rtTA/V5 (V5 control cell line) ...
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bioRxiv - Cancer Biology 2020Quote: ... Viral mixes were created with an aliquot of virus into complete media and polybrene at 8μg/ml (Sigma-Aldrich) to have a target multiplicity of infection (MOI ...
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bioRxiv - Immunology 2019Quote: Media was recovered approximately 48-hr post-transfection and cell-free virus stocks were generated by filtering the media through 0.45 µM filters (Millipore). The TZM-bl indicator cell line was used to quantify the amount of infectious virus(Derdeyn et al. ...
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bioRxiv - Biochemistry 2019Quote: ... First-strand cDNA was synthesized from 2 µg of total RNA using Moloney Murine Leukemia Virus Reverse Transcriptase (Sigma) and oligo dT (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... The activated cells were added to the virus-bound plates after removing dead cells using Histopaque-1119 (Sigma-Aldrich), and centrifuged at 3200 × g for 90 min at 32°C ...
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bioRxiv - Cancer Biology 2019Quote: Cells were lentivirally transduced by spinfection with centrifugation of cells with virus in the presence of 8μg/ml polybrene (Millipore) at 1,000xg for two hrs ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were seeded onto glass-bottom dishes and transduced with virus containing pEGFP_EB3 (gift from S. Godinho) Cells were treated with Eg5 (Kif11) inhibitor monastrol (67 μM, Sigma) for 2 hr ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RAMP1 expression achieved through transduction of virus containing RAMP1 MISSION TRC3 Open Reading Frame (ORF) plasmid (pLX_304) (Sigma, US) into RAMP2 KO-HUVECs ...
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bioRxiv - Synthetic Biology 2020Quote: ... the virus was removed and the cultures overlaid with a viscous solution of 1% (w/v) carboxymethylcellulose (Sigma #C4888) in DMEM-F12 (8% FBS ...