Labshake search
Citations for Millipore Sigma :
551 - 600 of 777 citations for CD3d Cynomolgus HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: ... for mFc tagged antigen versions or goat-anti-hIgG(Fc) conjugated with HRP (1:70000, A0170, Sigma) if hFc-tagged antigens had to be detected ...
-
bioRxiv - Bioengineering 2021Quote: ... and binding was detected with an anti-human IgG (Fc specific) (1:5000 dilution, Sigma-Aldrich, A0170) or anti-FLAG (1:1000 dilution ...
-
bioRxiv - Developmental Biology 2022Quote: ... A trapped bead was manually manipulated by a motorized XY stage (BIOS-225T and FC-101G, Sigma), and contact of the bead with the cilium during oscillation for 1.5 h was monitored by ciliary GCaMP6 or mCherry fluorescence imaging or bright-field imaging of the bead ...
-
bioRxiv - Cancer Biology 2019Quote: ... one cells have adhered remove culture medium and replace with diluted lentiviral media (500µl lentiviral media, 1.5ml DMEM, 10% FCS, 5% PenStrep, 5-10µg Polybrene (Sigma)) ...
-
bioRxiv - Immunology 2020Quote: ... Cells were washed and resuspended in RPMI supplemented with 10% FCS and 1% penicillin streptomycin (Sigma Aldrich) (complete media ...
-
bioRxiv - Pathology 2020Quote: ... stimulated for 24 hours in 10% FCS DMEM with LPS (1 µg/ml, Salmonella Minnesota; Sigma-Aldrich), BLP (Pam3Cys-Ser-(Lys)4 ...
-
bioRxiv - Systems Biology 2019Quote: ... Subsequently, cells were washed two times with a washing solution (5% FCS, 0.05% BSA (Sigma-Aldrich; A2058), 0.02% NaN3(Carl Roth GmbH & Co ...
-
bioRxiv - Developmental Biology 2021Quote: ... the embryos were harvested in sterile medium (10% FCS (Hyclone) and 1% pen/strep in PBS (Sigma)) ...
-
bioRxiv - Immunology 2021Quote: ... Medium was replaced every 3 days thereafter with fresh R+ containing FCS (10% v/v; Sigma-Aldrich). Antigen-specific CD8+ T cells were characterized via flow cytometry on day 10.
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Coding region of RBD-Fc was subcloned into a modified pCGS3 (Merck/formally known as Sigma-Aldrich) for glutamine selection in CHOZN GS-/- cells ...
-
bioRxiv - Immunology 2022Quote: ... 1*105 cells in 200μl/well complete T-cell medium (RPMI1640 supplemented with 10% FCS (Sigma/Biowest), antibiotics [PenStrep] ...
-
bioRxiv - Immunology 2023Quote: ... supplemented with 10% (v/v) heat-inactived fetal calf serum (FCS, Sigma-Aldrich, Saint Louis, MO, USA), 400 µg/ml G418 (Carl Roth ...
-
bioRxiv - Immunology 2023Quote: ... density centrifugation and cryopreserved in heat-inactivated foetal calf serum (FCS) supplemented with 10% DMSO (Sigma-Aldrich) in liquid nitrogen ...
-
bioRxiv - Immunology 2022Quote: ... 7 – 13×106 cells were washed in PBS with 2% FCS before incubation in 50nM dasatinib (Sigma) at 37°C for 30 minutes ...
-
bioRxiv - Bioengineering 2023Quote: ... Antibodies and dyes used in this study were: Anti-Human IgG Fc specific (Millipore Sigma I2136-1ML), Anti-6X His tag (Abcam ab9108) ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... including Phosphate Buffered Saline (PBS) and Fetal Calf Serum (FCS) were purchased from Sigma Aldrich (Gillingham, UK), except for blastocidin which was obtained from Gibco™ (MA ...
-
bioRxiv - Biochemistry 2024Quote: ... 2 mM glutamine and 10% dialyzed FCS after addition of 25 mM of either 12C-glucose (Sigma) or U-13C-glucose (Cambridge Isotopes ...
-
bioRxiv - Immunology 2021Quote: ... and 10% (v/v) fetal calve serum (FCS)) with 1.6 mg collagenase (Sigma-Aldrich, St. Louis, MO, USA) and processed twice on a GentleMacs using the lung program (Miltenyi Biotec ...
-
bioRxiv - Molecular Biology 2021Quote: ... supplemented with pyruvate and glutamine (Difco) and 10% (v/v) heat-inactivated foetal calf serum (FCS) (Sigma-Aldrich) (D10) ...
-
bioRxiv - Cell Biology 2022Quote: ... was maintained with Dulbecco’s minimal essential medium (DMEM) (Nacalai tesque) containing 10% fatal calf serum (FCS; Sigma-Aldrich). To differentiate the cells into myotubes ...
-
bioRxiv - Immunology 2020Quote: ... supplemented with 10% FCS (Eurobio, Les Ulis, France) and 20 µM beta-mercaptoethanol (SIGMA, Saint Quentin Fallavier, France)30 ...
-
bioRxiv - Bioengineering 2019Quote: ... tissue culture polystyrene culture plates were pre-coated with 20 mg/ml anti-IgG (human, Fc-specific, Sigma) for 1 hour at 37°C ...
-
bioRxiv - Microbiology 2019Quote: ... Fc chimeric proteins were purified via affinity based Fast Protein Liquid chromatography using Prosep® Ultra resin (Millipore). Fc conjugated proteins were eluted with 0.1 M glycine (pH 2.5 ...
-
bioRxiv - Biochemistry 2021Quote: ... the ACE2-Fc expression vector was transfected into Expi293T cells according to the manufacturer’s instructions (Sigma-Aldrich; L3287). Five days after transfection the supernatant was collected by first spinning cultures at 300 g for 5 min at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... Spinal cord specimens were then placed in glass tubes filled with Fluorinert (FC-770, Milipore Sigma, Darmstadt, Germany) for imaging ...
-
bioRxiv - Molecular Biology 2020Quote: ... The Bruce-4 cell line was maintained in DMEM supplemented with 15% FCS and ESGRO (Merck Millipore, UK) on a 0.1% gelatine-coated dish at 10% CO2.
-
bioRxiv - Microbiology 2020Quote: ... We detected bound antibody with horseradish peroxidase (HRP)-coupled anti-human (Fc) antibody (Sigma Aldrich catalog number A0170).
-
bioRxiv - Neuroscience 2020Quote: ... Medium supplemented with the exosome-depleted FCS and antibiotics was subsequently filtered through a 0.22 and a 0.1 µM filter-sterilization device (Millipore). For EV isolation ...
-
bioRxiv - Bioengineering 2020Quote: ... Tumor tissue was dissected into fragments of 1-2mm3 and frozen in 90% fetal calf serum (FCS, Sigma) and 10% dimethyl sulfoxide (DMSO ...
-
bioRxiv - Neuroscience 2022Quote: ... Medium supplemented with EV-depleted FCS and antibiotics was subsequently filtered using 0.22 and a 0.1 µM filter-sterilization devices (Millipore). For EV isolation ...
-
bioRxiv - Immunology 2019Quote: ... Monocytes were cultured in RPMI-1640 medium supplemented with 10% FCS and 1% sodium pyruvate (all from Sigma) in ultra-low attachment 6-well plates ...
-
bioRxiv - Immunology 2019Quote: ... cells were counted at d0 and 0.4x106 cells/ml were seeded in differentiation medium (RPMI supplemented with 2.5 % FCS, 0.5% dimethylformamide (DMF, D4551, Sigma), L-glutamine and antibiotics) ...
-
bioRxiv - Molecular Biology 2021Quote: Wildtype and ΔMETTL3 mES cells were maintained in DMEM supplemented with 15% FCS (ES-009-B, Millipore Sigma), 1X non-essential amino acids (11-140-050 ...
-
bioRxiv - Genetics 2021Quote: ... They were transferred to digestion medium (HBSS with Ca2+ Mg2+, FCS 2%, DNase I 0.5mg/mL (Sigma-Aldrich) and collagenase IV 0.5mg/mL (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2020Quote: ... The EV-containing eluates were pooled and supplemented with 10% heat-inactivated fetal calf serum (FCS; Sigma-Aldrich), or 0.1% BSA (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2021Quote: ... Cells were washed and resuspended in PBS containing 0.5 % FCS and subjected to FACS analysis (Guava EasyCyte, Millipore).
-
bioRxiv - Cancer Biology 2023Quote: ... Immunoreactive bands were visualised on Licor Odyssey Fc (Cambridge, UK) using enhanced chemiluminescence (ECL, Merck Millipore, Darmstadt, Germany). β-actin was used a loading control.
-
bioRxiv - Immunology 2023Quote: ... Surface staining was performed for 30 min at 4 °C in PBS supplemented with 2% FCS (Sigma-Aldrich) and 0.01% azide (Sigma-Aldrich ...
-
bioRxiv - Immunology 2023Quote: ... 2% FCS and 50 μg/ml Lipopolysaccharide neutralizing agent Polymyxin B sulfate (cat # 1405-20-5; Sigma-Aldrich) for 30 min at 37°C while shaking by 750 rpm on a heating block ...
-
bioRxiv - Cell Biology 2023Quote: ... Sorted single cells were collected in ADF plus 10% FCS and 9 µM Rock inhibitor (Y-27632, Sigma) to prevent anoikis ...
-
bioRxiv - Genetics 2023Quote: ... supplemented with 10% fetal calf serum (FCS, Capricorn, FBS-12A) and 1% penicillin/streptomycin (P/S, Sigma, P0781). The cultured fibroblasts were incubated at 37°C with 5% CO2.
-
bioRxiv - Cancer Biology 2022Quote: Expression of indicated Flag-tagged CDK6 constructs in HEK293 cells were determined via Western blotting with indicated Flag antibody [mouse anti-FLAG® M2-tag (Sigma-Aldrich, St. Louis, MO, USA, F3165-1MG)] ...
-
bioRxiv - Biochemistry 2021Quote: ... cells were cultured for at least six days in RPMI 1640 Medium supplemented with 10% dialyzed FCS (Sigma Aldrich), containing either “heavy” lysine (40 g/l ...
-
bioRxiv - Biophysics 2020Quote: ... Cytochalasin D (from Zygosporium masonii) as well as fetal calf serum (FCS) were from Sigma Aldrich (Merck KGaA, Germany). DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The HE-Fc containing fractions were dialyzed in PBS and concentrated using 30kD Amicon Ultra-15 filters (EMD Millipore). Purified proteins were observed by SDS gel migration as well as anti-human IgG Fc probing by Western ...
-
bioRxiv - Developmental Biology 2019Quote: ... media was replaced with DMEM/F12 containing 100ng/ml Activin A and 0.2% foetal calf serum (FCS, Sigma-Aldrich) for a further 24 hours ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were grown in DMEM (gibco) with 1 g/mL glucose (MCEC) or 4.5 g/mL (HUVEC, HeLa, MCF-7) containing 10% FCS (Sigma), 1% penicillin (10000 Units/ml ...
-
bioRxiv - Bioengineering 2021Quote: ... Cells were seeded at 1E6 cells/ml in MEM-Alpha (Biological Industries) supplemented with 10% Heat Inactivated FCS (Sigma) and 50IU rhIL-2 (Peprotech).
-
bioRxiv - Cancer Biology 2020Quote: ... MDA-MB-231 cells were cultured in DMEM medium (Pan-Biotech, Germany) supplemented with 10% FCS (Sigma-Aldrich, Germany), 2 mM L-glutamine (Pan-Biotech ...
-
bioRxiv - Biophysics 2020Quote: All used BMPs and extracellular domains (ECD) of the BMPR-FC chimeras were bought from Sigma Aldrich (Missouri, USA) and R&D systems (Minnesota ...