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Citations for Millipore Sigma :
701 - 750 of 818 citations for CD3d Cynomolgus HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... USA) cells and the respective sublines were maintained in RPMI-1640 medium containing 8% fetal calf serum (FCS) (Sigma, F7524), 2 mM L-glutamine ...
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bioRxiv - Immunology 2023Quote: ... 8129) cells were maintained in Dulbecco Modified Eagle Medium (DMEM) supplemented with 10% fetal calf serum (FCS, Sigma cat. F7524) and 100 U/ml of penicillin/streptomycin ...
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bioRxiv - Physiology 2023Quote: ... Supernatants from cells overexpressing Fc-tagged FGL1 proteins were collected after 5 days and supplemented with protease inhibitor cocktail (Sigma). Recombinant proteins were purified using Hitrap Protein A HP column on an ÄKTA pure chromatography system (GE healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... Following secondary antibodies at the indicated dilution were used for IF: Anti-rabbit IgG (Fc) TRITC (Sigma #SAB3700846, 1:500), Anti-mouse IgG (Fc ...
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bioRxiv - Biochemistry 2023Quote: ... the Siglec-7-Spy Tag-TEV-Fc was concentrated using an AMICON (Molecular Weight Cut Off 30 kDa – Merck Millipore) to approximately 0.15 mg/ml measured via NanoDrop as described above ...
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A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... cells were dissociated by incubating the pooled tissue in 250gl PBS supplemented with 10% fetal fetal calf serum (FCS) and 1 mg/ml collagenase (Sigma) for 45 minutes at 37° C with shaking at 750 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... Inocula were removed and replaced with DMEM containing 1% FCS with a semi-solid overlay consisting of 1.5% carboxymethyl cellulose (Sigma-Aldrich). Cells were incubated for 4-5 days ...
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bioRxiv - Immunology 2023Quote: ... Pellets were recovered in PBS 2% FCS and erythrocytes were removed using a red blood cell lysis buffer (Sigma-Aldrich) before being filtered onto a 40-µm cell strainer (Dutscher) ...
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bioRxiv - Immunology 2024Quote: ... the cells were stained for 30 min on ice in PBS containing 2% FCS and 0.1% sodium azide (cat. S2002, Sigma-Aldrich). To examine intracellular cytokine production ...
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bioRxiv - Immunology 2024Quote: ... All bacteria cultures were then centrifuged at 3,000 rpm for 10 min and the bacterial pellet was resuspended into 300μl of RPMI/5% FCS with gentamycin (Sigma Aldrich) (30 μg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... The proximal forelimbs of embryos were dissected (see Figure 3) and placed into PBS with 10% FCS and 8ul collagenase at 50mg/ml (Sigma C9697) at 37° for approximately 5min ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with 3-isobutylmethyl-xanthine (1 mM)-containing medium or including the secondary antibody anti-mouse IgG (Fc specific, M2650, Sigma-Aldrich) for 30 min ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed six times and incubated with 100 μL/well horseradish peroxidase (HRP)-conjugated goat anti-human IgG (Fc-specific, Sigma Aldrich) diluted 1/5000 in PTA buffer ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed four times with PBST and 100 μl/well of goat anti-human immunoglobulins-peroxidase IgG (FC specific; Sigma-Aldrich), IgM (μ-chain specific ...
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bioRxiv - Microbiology 2019Quote: ... (ATCC) cells were maintained in DF-1 cell culture media (DMEM supplemented with 10% FCS, 5% tryptose phosphate broth (Sigma-Aldrich), 1% NEAAs and 1% penicillin-streptomycin and maintained at 39 °C in a 5% CO2 atmosphere.
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bioRxiv - Cell Biology 2019Quote: ... purified DN3a cells were cultured on OP9-DL1 stromal cells or Fc-DL1 functionalised surfaces in the presence of AMD3100 (2 μg/ml, Sigma-Aldrich) or DBZ (0.05 µM ...
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bioRxiv - Genetics 2021Quote: ... EDL muscles were digested in Krebs solution (without FCS) containing 3 mg/ml collagenase type IV A (Sigma Aldrich, MO, USA), gently bubbled with carbogen (95% O2 ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10 % Foetal Calf Serum (FCS, LabTech International Ltd, Heathfield, East Sussex, UK) and 4 mM L-glutamine (Sigma-Aldrich).
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bioRxiv - Biochemistry 2020Quote: ... and Anti-Mouse IgG (Fc specific)-FITC antibody produced in goat (Cat # F4143, Sigma Aldrich, USA; 30 min at 4°C).
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bioRxiv - Neuroscience 2021Quote: 4% PFA-fixed brains were washed twice in 20 mL PBS for a total of 24 hours and imaged in Fluorinert FC-40 (Sigma Aldrich) for null background signal ...
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bioRxiv - Bioengineering 2021Quote: Each intact piglet brain hemisphere was placed in a 30 mm diameter glass tube containing perfluorocarbon oil (Fluorinert FC-40, Sigma-Aldrich) as a magnetic susceptibility matching fluid ...
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bioRxiv - Microbiology 2020Quote: ... Then cells were resuspended to 100 μl PBSA buffer followed by addition of 1 μl PE conjugated anti-human Fc antibody (Sigma-Aldrich) and incubate at 4 °C for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... gondii parasites were cultured in complete medium (DMEM, +5 % FCS) or in custom culture medium −Pan (DMEM −Pan, +5 % dialyzed FBS) supplemented with Val (Sigma, V6504), pantoate (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Thymic fragments were washed in RPMI 2% FCS and digested 15 min at 37 °C in 500 μg/mL Liberase TL (Sigma-Aldrich) and 111 μg/mL DNase I from bovine pancreas (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2020Quote: ... in 20 ml Dulbecco’s Modified Eagle Medium (DMEM, Capricorn, Ebsdorfergrund, Germany) containing 10 % fetal calf serum (FCS, Sigma, St. Louis, USA) and 1 % penicillin-streptomycin (Gibco ...
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bioRxiv - Immunology 2022Quote: ... Any larger tissue fragments were dissociated mechanically followed by washing of the cell strainer with MACS buffer (PBS, 2% FCS, 2mM EDTA (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2021Quote: ... Wells were washed 3 times with washing buffer then 100 μL of diluted goat anti-mouse IgG (Fc specific)-Peroxidase antibody (#A0168; Sigma-aldrich) were added ...
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bioRxiv - Microbiology 2022Quote: ... Plates were washed 4 times with PBS/Tween and then incubated for 1 h at RT with 100 μL/well of an anti-mouse Fc conjugated to alkaline phosphatase (Sigma-Aldrich), diluted 1:1000 in blocking buffer ...
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bioRxiv - Immunology 2022Quote: Each SMG pair (approx. 160mg) was mechanically minced in 2mL of RPMI-1640 containing 5% fetal calf serum (FCS; Sigma Aldrich), 25µl Collagenase-II (23mg/mL ...
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bioRxiv - Immunology 2019Quote: ... Cells were plated at 0.5-1 × 105 cells in a 96 well plate in L-15 media containing 5% FCS and exposed to DMSO (vehicle control) or 50 ug/ml 17-β estradiol or Bis-phenol A (Sigma) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... Afterwards the medium was switched and cells were cultivated for 4 days in DMEM containing 10% FCS and 2 μM insulin (Sigma Aldrich) to induce adipogenic differentiation ...
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bioRxiv - Pathology 2020Quote: SFs were seeded in 6-well plates at 1×106 cells per well and synchronised overnight in 1% FCS DMEM before being treated for 24 hours with the indicated stimuli: LPS (1 µg/ml, Sigma-Aldrich), IL-1β (10ng/ml ...
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bioRxiv - Cell Biology 2021Quote: ... [42] After expansion in complete A-RPMI, pUC were cryopreserved in heat inactivated fetal calf serum (FCS, Biochrom) supplemented with 10 % Dimethyl sulfoxide (DMSO, Sigma Aldrich) until use ...
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bioRxiv - Immunology 2020Quote: ... cells were expanded in selection medium (10 % dialysed FCS, 1x GSEM supplement [Sigma-Aldrich], 20–50 μM MSX, 1 % Pen/Strep) for at least 1 week ...
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bioRxiv - Immunology 2021Quote: ... cells were expanded in selection medium (10 % dialysed FCS, 1x GSEM supplement [Sigma-Aldrich], 20–50 µM MSX, 1 % Pen/Strep) for at least 1 week ...
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bioRxiv - Immunology 2021Quote: ... 721.221 was purchased from the International Histocompatibility Working Group and grown in RPMI-1640 supplemented with 15% heat-inactivated fetal calf serum (FCS) (Sigma-Aldrich), sodium pyruvate (Thermo Fisher) ...
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bioRxiv - Immunology 2020Quote: Urine cells were thawed into 9 ml of 10% FCS-RPMI medium supplemented with 10 IU/ml Benzonase (Novagen 70664-3), counted and viability determined by trypan blue exclusion ...
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bioRxiv - Immunology 2019Quote: Small intestinal biopsies were collected at the time of endoscopy and transported in R10 (RPMI-1640 [Lonza] + 10% FCS [Sigma-Aldrich] + 1% penicillin/streptomycin [Sigma-Aldrich]) ...
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bioRxiv - Cell Biology 2020Quote: ... OMP-18R5 (OncoMed Pharmaceuticals, Redwood City, CA) at 10μg/mL with antihuman IgG (Fc) secondary conjugated to FITC (Millipore Sigma AP113F).
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bioRxiv - Immunology 2020Quote: ... Freshly isolated PBMCs from four donor M1/M2 MCM were pooled and resuspended in RPMI-1640 containing 10% fetal calf serum (FCS; R10) and 5 ug/ml phytohemagglutinin-P (Sigma-Aldrich). The culture was incubated overnight at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Immunology 2020Quote: ... and 2.5 ng/mL TNF-α for 3-5 days and subsequent differentiation in RPMI (Sigma, St Louis, Mo; +10% FCS) either with 100 ng/mL GM-CSF ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used are as follows: Anti-poly-ADP-ribose binding reagent/ PAR reagent (recombinant protein fused to rabbit Fc tag; Millipore, MABE1031), Rabbit monoclonal anti-Poly/Mono-ADP Ribose (anti-PAR/MAR ...
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bioRxiv - Immunology 2022Quote: ... fibroblasts and MCF 10A cells were incubated with the FITC bound anti-mouse IgG secondary immunoglobulin (Fc specific) (Cat # F4143, Sigma-Aldrich) secondary antibody for 2 h at room temperature away from light ...
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bioRxiv - Cell Biology 2022Quote: ... A HeLa cell line stably expressing connexin43 [47] grown in DMEM/10%FCS/P/S supplemented with 1 μg/ml puromycin (Sigma #P9620) were solubilized in 1% Chaps/TBS/5% glycerol at pH 7.4 supplemented with protease blockers ...
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bioRxiv - Synthetic Biology 2022Quote: ... Single-cell suspensions were derived by mechanical disruption of bone marrow in PBS supplemented with 2% Fetal Calf Serum (FCS, Sigma, C8056). For FACS analysis ...
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bioRxiv - Microbiology 2022Quote: ... and suspended at 2 x 104 cells/mL in Titan Cell Medium (TCM) composed by 5% Sabouraud, 5% inactivated Fetal Calf Serum (FCS, Biological Industries) and 15 µM sodium azide (Sigma Aldrich), diluted in 50 mM MOPS adjusted to pH 7.3 ...
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bioRxiv - Microbiology 2022Quote: MPXV strains were diluted ½ in two different kinds of media that is Viral Transport Media (VTM; 330C, Copan) and FCS (F2442, Sigma-Aldrich). Two hundreds microliters of each sample were submitted in triplicate to various temperatures for different periods of time ...
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bioRxiv - Molecular Biology 2024Quote: ... Negative selection was therefore carried out by culturing the parasites in the presence of 5-fluorocytosine (5-FC, Sigma-Aldrich, UK) at 48 mM and 2.5 nM WR99210 for a week ...
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bioRxiv - Immunology 2024Quote: ... sections were washed with PBS to remove OCT compound on the slides, and blocked with solutions containing imaging buffer (2% FCS, 0.1% Triton X-100 (Sigma, Cat. X100), 0.01% sodium azide) ...
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bioRxiv - Microbiology 2024Quote: ... and treated with 16 μM Hoechst 33342 and/or Anti-Human IgG (Fc specific)−FITC antibody (1:100 dilution, Sigma-Aldrich) in iCM ...