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Citations for Millipore Sigma :
5901 - 5950 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... The fractions containing the capsid protein were pooled and concentrated using an Amicon Ultra-15 centrifugal filter (Merck Millipore), and the buffer was exchanged to a final storage buffer composed of 50 mM NaH2PO4/Na2HPO4 ...
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bioRxiv - Biophysics 2021Quote: ... The final purified proteins were concentrated using 50 kDa (MtCNNMΔC) or 100 kDa (MtCNNM) cutoff concentrators (Amicon Ultra, Millipore), flash frozen in liquid nitrogen ...
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bioRxiv - Biophysics 2021Quote: ... Peak fractions corresponding to soluble protein were pooled and concentrated to 4.5–5.5 mg/mL (Amicon Ultra 100-kDa cut off, Millipore Sigma). Protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (Implen Nanophotometer N60 ...
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bioRxiv - Biophysics 2021Quote: ... Further purification was achieved through capture of the cleaved protein by Strep-Tactin resin (IBA) and elution with 2.5mM d-desthiobiotin (Sigma). A final purification step was performed by size-exclusion chromatography using a HiLoad 16/60 Superdex 75 column (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: The protein was thawed and concentrate at 10 mg/ml in Amicon Ultra-15,10000Mr cut-off centrifugal concentrator (Millipore). 10 mM shikonin was added to the protein in a ratio of 5:1 and bond at 4°C for 2h ...
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bioRxiv - Biophysics 2022Quote: ... Protein was concentrated up to 40–60 mg/ml using a 100 kDa molecular weight cut-off concentrator (Millipore). The protein purity was checked by SDS-PAGE ...
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bioRxiv - Microbiology 2022Quote: ... The protein was purified affinity chromatography using Ni-NTA column (Yeasen, Wuhan, China) and concentrated using centrifugal ultrafiltration (Millipore). The concentration of recombinant protein was determined with bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2022Quote: ... Non-specific protein adsorption to the coverslip was prevented by adding 0.005% (v/v) Tween-20 (P2287, Sigma-Aldrich) to the solution ...
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bioRxiv - Immunology 2022Quote: ... up to 5 × 107 cells were stained with monoclonal antibodies (Table 1) to cell surface proteins diluted in PBS containing 1 % BSA (Bovogen) and 0.1 % NaN3 (Sigma) (staining buffer ...
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bioRxiv - Microbiology 2022Quote: RNA immunoprecipitation (RIP) assays were performed using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (EMD Millipore 17-700). HEK293T cells were plated on 15-cm dishes coated with poly-L-ornithine at a density of 4E4 cells/cm2 ...
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bioRxiv - Biochemistry 2022Quote: ... assay and immediately diluted (1:1) to a final protein concentration of 0.5 mg/mL with either 18O heavy (Sigma) or 16O light (Fisher ...
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Dissociation of β2m from MHC Class I Triggers Formation of Noncovalent, Transient Heavy Chain DimersbioRxiv - Cell Biology 2022Quote: ... were used at 14,000 x g and 4 °C to exchange purified protein samples to 150 mM ammonium acetate (99.99 %; Sigma-Aldrich), pH 7.2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Argonaute proteins/small RNA complex were extracted from 50,000 synchronized worms and immunoprecipitated with anti-flag antibody (Sigma Aldrich) or GFP nanobody (Thermo) ...
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bioRxiv - Microbiology 2022Quote: ... of 3.5 mg/mL mixtures of purified STFK or STFK1628x proteins in complex with excess Fab fragments of nAbs were incubated in 0.01% (v/v) Digitonin (Sigma) and then loaded onto glow-discharged (60 s at 20 mA ...
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bioRxiv - Microbiology 2022Quote: Poly-histidine (His6)-tagged proteins were expressed and purified by affinity chromatography using Ni-NTA agarose beads (Novagen, USA) according to the manufacturer-recommended instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were separated by SDS-PAGE with 8% polyacrylamide gel and transferred to PVDF membrane (Millipore, Billerica, MA, USA). Membranes were blocked with 5% skim milk in Tris-buffered saline (20 mM Tris [pH8.0] ...
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bioRxiv - Immunology 2022Quote: Antibodies against the following proteins were obtained from commercial sources and used for immunoblots: anti-flag-tag (F3165, Sigma), anti-V5-tag (R960-25 ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell types were probed with anti-Pan cytokeratin (Cat # 4545, Cell signalling technology, U.S.A) and anti-Fibroblast specific protein (Cat # F4771, Sigma, U.S.A) as primary antibodies ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions of monomeric protein were collected and concentrated to around 30-50 µM using Amicon Ultra-0.5 ml Centrifugal Filters (Millipore), then separated into single-use aliquots ...
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bioRxiv - Bioengineering 2022Quote: ... The prenylated protein was subsequently purified by buffer exchange (PBS) with an Amicon Ultra-0.5 centrifugal filter (10 kDa cutoff, Millipore) for cell surface modification or the following conjugation reactions.
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bioRxiv - Bioengineering 2022Quote: The proteins and protein conjugates were washed into ultrapure water with the Amicon Ultra-0.5 centrifugal filters (10 kDa cutoff, Millipore). 50 μL of each protein (5 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were eluted by incubating the beads with 35 μl 100 µg/ml 3xFLAG-Peptide (F4799, Sigma Aldrich) at room temperature for 20 min or by adding Laemmli Sample Buffer (1610747 ...
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bioRxiv - Biochemistry 2022Quote: ... The two aqueous streams were supplied with the cell solution and with a 3 μM substrate solution containing lysis agents (0.7× BugBuster protein extraction reagent, Merck Millipore; 60 kU/mL rLysozyme, Novagen) in droplet assay buffer ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was recovered and the protein concentration was estimated using the Direct Detect® Infrared Spectrophotometer (Merck-Millipore). 400 µg protein were incubated with 40 µL of streptavidin coupled DynabeadsTM M-280 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Protein extracts were separated by SDS-polyacrylamide gel electrophoresis then transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Temecula, CA). Primary antibodies and secondary antibodies were used according to manufacturers’ instructions ...
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bioRxiv - Immunology 2020Quote: ... The protein-containing supernatants from cells expressing HA-I53_dn5B fusion proteins were further clarified by vacuum filtration (0.22 µm, Millipore Sigma). Prior to immobilized metal affinity chromatography ...
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bioRxiv - Immunology 2020Quote: ... The separated proteins were transferred to PVDF membranes and blocked in PBS containing 0.2% fish skin gelatin (Sigma-Aldrich) and 0.1% Tween-20 ...
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bioRxiv - Microbiology 2020Quote: ... the mice were injected with 700 μg of His-tagged TGT complex protein in incomplete Freund’s adjuvant (Sigma-Aldrich). One week after the 6-week injection ...
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bioRxiv - Biochemistry 2021Quote: ... After elution with imidazole the purified protein was concentrated using a 10 kDa MW cutoff Amicon centrifugal filter (Sigma), buffer exchanged into PBS (10mM Na2HPO4 ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein pellet was washed 3 times in a solution of 0.3 M guanidine hydrochloride (Sigma, Cat. No. SRE0066) in 95% ethanol ...
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bioRxiv - Neuroscience 2019Quote: ... proteins were reduced with 20 mM dithiothreitol and free cystein residues were alkylated with 50 mM iodoacetamide (Sigma Aldrich). After the urea washing steps ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant containing Galectin-3 protein was treated with benzamidine sepharose (HiTrap Benzamidine FF, Sigma, Cat# GE17-5143-02) to bind and remove thrombin ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies to the N protein was detected by incubation for 1h at 37°C with anti-human IgG peroxidase (1:30,000, Sigma). The plates were washed three times with PBS with 0,05% Tween-20 between each step ...
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bioRxiv - Developmental Biology 2020Quote: ... the supernatant was subjected to an immunoreaction with a specific antibody pre-conjugated with magnetic protein A beads (Millipore) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were blocked with 3% w/v BSA and incubated with 1:16,000 v/v rabbit anti-protein A antibody (SIGMA) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... Fractions containing the complex were concentrated to 0.12 mg/ml and proteins were crosslinked by addition of 0.05% (v/v) glutaraldehyde (Sigma Aldrich) and incubated at room temperature for 60 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Extracted proteins were then separated via SDS-PAGE and then transferred to a 0.45 μM polyvinylidene fluoride (PVDF) membrane (Millipore) on a TRANS-BLOT SD apparatus (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... Total protein content in each eluate was measured using infra-red spectroscopy (Direct Detect, Merck Millipore, Burlington, MA, USA). The remainder was aliquoted and stored at −80 °C.
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bioRxiv - Molecular Biology 2020Quote: ... equal amounts of total protein were separated by 10% SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). The membranes were blocked and incubated with primary antibodies against FGF2 (36769 ...
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bioRxiv - Molecular Biology 2020Quote: ... the His6-tag-fusion proteins were purified from the supernatant by a HIS-Select® Nickel Affinity Gel (Sigma) column ...
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bioRxiv - Biochemistry 2021Quote: ... Peak fractions corresponding to soluble protein were pooled and concentrated to 4.5 - 5.5 mg/mL (Amicon Ultra 100 kDa cut off, Millipore Sigma). Protein samples were flash-frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Microbiology 2020Quote: ... For elution of FLAG-tagged proteins and their complexes 100-300 µL of 0.1 mg/mL FLAG3 peptide (Sigma) was added to samples ...
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bioRxiv - Cancer Biology 2019Quote: ... a sample mixture of 10 μg protein and non-reducing sample buffer was loaded on a polyacrylamide gel containing gelatin (0.25 mg/ml; Sigma). The gel was then soaked (36 h ...
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bioRxiv - Immunology 2020Quote: Western blot analysis of protein levels was performed on whole cell lysates prepared by lysis in RIPA buffer (Sigma) supplemented with Complete EDTA free proteinase inhibitors (Roche) ...
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bioRxiv - Cancer Biology 2022Quote: ... the protein extracts (15 μg/sample) were blotted on a primed 0.45 μm polyvinylidene fluoride (PVDF) membrane (Merck-Millipore). Protein spots were stained using Direct Blue (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins samples (20 μg/lane) were separated by SDS–PAGE and transferred onto PVDF membranes (0.45μm, Millipore, USA) using a transfer unit (Bio–RAD ...
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bioRxiv - Genomics 2022Quote: ... Proteins from whole cell lysate were separated in Laemmli buffer by SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked in 5% milk in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Immunology 2022Quote: ... Protein lysates were separated on 10% SDS-PAGE gels and then transferred to PVDF membranes (Millipore, Billerica, MA, USA). The membranes were blocked with 5% nonfat dry milk at room temperature for 2 hours and then incubated at 4°C overnight with primary antibodies against β-tubulin (Abcam ...
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bioRxiv - Immunology 2022Quote: ... DENV infection was then measured by intracellular detection of the E protein using the murine monoclonal 4G2 antibody (Millipore) followed by rabbit anti-mouse IgG-coupled to AF647 (Molecular probes) ...
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bioRxiv - Cancer Biology 2022Quote: ... equal protein amounts were collected across the samples and subjected to 25 μM DBCO-PEG4-biotin (Sigma-Aldrich, #760749) at room temperature for 30 min ...