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Citations for Millipore Sigma :
5751 - 5800 of 7654 citations for Parvovirus VP2 Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... the 6×His tag on the proteins was further cleaved with Thrombin CleanCleave kit (Sigma-Aldrich, Cat# RECMT) overnight at 4 °C on the rocking table after desaltig in 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: The culture supernatants containing the secreted proteins were centrifugated (1 h, 30,000xg, 10 °C) and sterilized by filtration (0.22 µm; Express Plus; Merck Millipore). The supernatants were equilibrated to pH 7 with 0.5 mM NaOH and the proteins were purified using two ion exchange chromatographies (Fig ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were eluted from the beads by adding 25 μL of Laemli Sample Buffer 2X (Sigma-Aldrich, #S3401), before heating tubes at 95°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 10-50 μg total protein was fractionated by SDS-PAGE and transferred to PVDF or nitrocellulose membranes (Millipore). Blocking was achieved with 5% milk in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Biophysics 2024Quote: ... Oligomeric α-Syn species were removed by filtering the protein through a 50-kD cutoff column (Amicon, Millipore). Aliquots of the protein were prepared ...
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bioRxiv - Biochemistry 2024Quote: ... The cleaved protein was collected and concentrated using 100 kDa MW cut-off spin concentrators (Amicon Merck-Millipore) separated by size-exclusion chromatography (SEC) ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted protein fractions were pooled and concentrated using a 10 kDa cut-off (Amicon filter, Millipore, USA). After affinity purification ...
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bioRxiv - Microbiology 2024Quote: ... Samples normalized for the protein content were separated by SDS-PAGE and transferred onto a PVDF membrane (Millipore). After washing with TBS buffer twice ...
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bioRxiv - Cancer Biology 2024Quote: ... Proliferating AT2 cells were identified by immunofluorescence for the AT2 marker surfactant protein-C (SPC, EMD Millipore, #AB3786) and proliferation maker Ki67 (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... Total protein extracts were fractioned on SDS-PAGE and examined by Western blot with mouse anti-myc (Sigma), mouse anti-Pgk1 (Thermofisher) ...
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bioRxiv - Microbiology 2024Quote: Fifteen amino acid (aa) peptides overlapping 12 aa spanning the structural proteins of CHIKV were purchased from Sigma and were divided into 4 overlapping peptide pools ...
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bioRxiv - Immunology 2024Quote: ... Protein was eluted from Sepharose A by addition of 35 μL sample loading buffer (Sigma-Aldrich, S3401-10) and incubation at 80 °C for 5 min ...
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bioRxiv - Microbiology 2024Quote: ... samples were thawed and 10 µl of total protein extract was added to 100 µM nitrocefin (Sigma-Aldrich) solution in PIPES 2 mM pH 6.8 and substrate hydrolysis measured spectrophotometrically over 30 minutes (Cytation5 ...
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bioRxiv - Molecular Biology 2024Quote: ... we used commercially available ELISA kits: hs-CRP Human C-Reactive Protein ELISA Kit (#RAB0096, Sigma-Aldrich, Germany) and Mercodia Insulin ELISA (#10-1113-01 ...
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bioRxiv - Plant Biology 2024Quote: ... Protein concentrations of the cell lysate or EV pellets were quantified with Pierce 660 nm reagent (Sigma #22660) using the manufacturer’s protocol and comparing to a bovine serum albumin standard curve.
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bioRxiv - Genetics 2024Quote: ... complex was prepared 15 min before transfection by mixing gRNAs (1.5 µM) with Cas9 protein (0.45 µM) (Sigma, Catalog no ...
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bioRxiv - Immunology 2024Quote: ... that contained protein: carbohydrate: fat (20:20:60, kcal%) plus high fructose (23.1 g/L; F3510, Sigma,USA) and glucose (18.9 g/L ...
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bioRxiv - Cell Biology 2024Quote: ... 10-30 μg of protein was resolved by electrophoresis and then transferred to a polyvinylidene difluoride membrane (Millipore). Membranes were blocked in TRIS-buffered saline with 0.1% tween 20 and 5% BSA for 1 hour and then incubated overnight with primary antibody at a dilution of 1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of total protein were loaded onto an SDS-PAGE before transfer onto a nitrocellulose membrane (Millipore). The membranes were blocked in 5% (w/v ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Fractions containing the protein were pooled and concentrated with a 30 kDa cutoff Amicon centrifugal filter (Sigma UFC9030) and loaded onto a Superdex 200 size exclusion column equilibrated with the storage buffer (25mM HEPES pH 7.4 ...
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bioRxiv - Genetics 2024Quote: ... cell surface protein samples were concentrated into 0.1 ml volumes using a 30 kDa centrifugal concentrator (Merck Millipore).
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bioRxiv - Molecular Biology 2024Quote: ... For dTAG-13 induced protein degradation cells were treated with 500 nM dTAG-13 (Sigma-Aldrich, SML2601-5MG) or an equivalent volume of DMSO (Sigma-Aldrich ...
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bioRxiv - Pathology 2024Quote: ... 0.5% Triton and lysates were incubated with rabbit or mouse isotypic control antibodies and G-protein beads (Sigma) for 1 hr at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Collected cells were immediately spun down and resuspended in BugBuster® Protein Extraction reagent (Novagen, Merck and Co. Inc) to lyse the bacterial cells and release proteins ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated by 10% SDS-PAGE gel and transferred to 0.45 µm polyvinylidene difluoride (PVDF) membrane (Millipore, USA). After blocking by with 5% non-fat dry milk for 2h at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... and 3×FLAG::GFP-tagged protein and associated RNAs were eluted with 100 μg/mL 3×FLAG peptide (Sigma). The eluates were incubated with TRIzol reagent (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... 25 μL of magnetic Protein G Dynabeads were coated with 2 μg of M2 anti-FLAG antibody (F1804, Sigma) for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2020Quote: Proteins were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride membranes (Millipore). After in-cubated with antibodies ...
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bioRxiv - Cell Biology 2019Quote: Protein was extracted from isolated cell pellets or whole liver tissue using RIPA buffer with protease inhibitors (Sigma-Aldrich). Equal amounts of protein were loaded and separated by SDS-PAGE gel electrophoresis on 4%-20% Criterion gels (BioRad ...
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bioRxiv - Cell Biology 2019Quote: ... the extracted proteins were separated in 10% polyacrylamide gels (Schägger and von Jagow, 1987) and blotted onto Immobilion-Fl membranes (Millipore). The membranes were incubated in blocking solution (5% skim milk in TBST ...
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bioRxiv - Cell Biology 2019Quote: ... cells were serum starved for 48 hours and proteins were extracted with lysis buffer (Sigma Cell Lytic Solution C2978) with 0.1% CHAPSO ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein of cell extracts was determined by Bradford method and GST□BHMT was precipitated with glutathione agarose (Sigma). Precipitates were then mixed in SDS□PAGE sample buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Lamin A proteins were detected with mouse anti-myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
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bioRxiv - Microbiology 2020Quote: ... and FLAG-tagged proteins were purified using anti-FLAG M2 affinity gel (Sigma; mouse anti-FLAG clone M2 antibodies). For mass spectrometry sample preparation ...
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bioRxiv - Microbiology 2020Quote: ... The mitochondrial import assay results were analysed by visualizing the proteins with the Coomassie stain (PhastGel Blue R, Sigma). The proteins were then fixed (20% ethanol and 10% acetic acid) ...
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bioRxiv - Microbiology 2020Quote: ... 30 μg of total protein was analyzed by SDS-PAGE gel electrophoresis and transferred to a PVDF membrane (Millipore). The membrane was probed with antibodies at 4 °C overnight and visualized using Western Lightning™ Plus ECL (erkinElmer ...
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bioRxiv - Molecular Biology 2021Quote: Western blot analysis of protein levels was performed on whole cell lysates prepared by lysis in RIPA buffer (Sigma) supplemented with 1 mM DTT (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing Exo1 protein variant were pooled and applied to 0.3 mL of M2 anti-FLAG agarose beads (Sigma) in batch ...
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bioRxiv - Developmental Biology 2021Quote: ... The lysate was centrifuged to remove cell debris and then incubated with Protein G Sepharose Fast Flow (Sigma-Aldrich) for 1 h at 4°C to remove nonspecifically bound proteins ...
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bioRxiv - Genomics 2021Quote: ... Antibodies-chromatin complexes were then pulled-down using 20 μL Magna ChIP™ protein A+G magnetic beads (Millipore) by incubating with rotation (20 rpm ...
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bioRxiv - Microbiology 2021Quote: ... Sized proteins were brought to concentrations typically >50 mg mL−1 using appropriate molecular weight cut-off concentrators (Millipore), flash frozen on liquid nitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 ug to 200 ug of protein was run on a 7.5 or 10% SDS-PAGE gel and transferred onto a PVDF membrane (Sigma-Millipore). The membranes were blotted for 1 hr in 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... and 30µg to 50µg of total proteins were loaded onto 7.5% or 12% SDS-polyacrylamide gels for electrophoresis and transferred onto polyvinylidene difluoride membranes (Millipore). After 1h of blocking with 5% bovine serum albumin or non-fat milk prepared in Phosphate-Buffered Saline (PBS)-0.1% Tween-20 buffer ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The fluorescent proteins encoded on the plasmid also carries a gene for antibiotic resistance (Gentamicin 418, Sigma-Aldrich: G418). Cells were cultured in autoclaved HL5 medium (per L ...
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bioRxiv - Genetics 2022Quote: ... 25 μg chromatin was diluted with IP dilution buffer and pre-cleared with Protein A agarose beads (Merck-Millipore) for 1 h at 4°C ...
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bioRxiv - Neuroscience 2021Quote: Sections were washed in 0.01M PBS and then placed in primary antibody (NeuN monoclonal mouse anti-neuronal nuclear protein, Millipore MAB377 ...
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bioRxiv - Neuroscience 2021Quote: ... and performed western blotting analysis.Western blotting: Protein concentration of lysates was determined with Bicinchoninic Acid Kit (#BCA1, Sigma Aldrich). Equal amount of proteins was loaded on the poly-acrylamide gels ...
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bioRxiv - Molecular Biology 2021Quote: The protein concentrations of the lysates were determined by an amido black filtration assay56 with Fraction V BSA (Sigma) for a standard curve ...
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bioRxiv - Neuroscience 2021Quote: ... FLAG-tagged protein was detected by an anti-FLAG M2 antibody conjugated with horseradish peroxidase (A8592, Sigma-Aldrich, U.S.A.). CaMKIIβ and α-Tublin were detected by primary antibodies anti-CaMK2β (#139800 ...