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Citations for Millipore Sigma :
5751 - 5800 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... protein were combined with a mixture of 50 % (v/v) protein A and 50 % (v/v) protein G beads (Sigma-Aldrich Inc., Cat#P9424 and Cat#P3296) and 0.5-1 μg antibody in lysis buffer overnight at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: Mitochondrial mass was assessed by measuring citrate synthase activity from 4 million whole cells normalized to protein input (U/mg) in triplicate from three biological replicates using the Citrate Synthase Assay Kit (CS0720, Sigma-Aldrich, St, Louis, MO).
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bioRxiv - Molecular Biology 2023Quote: ... the protein solution was dialyzed overnight at 4°C in tubes of the Pur-A-LyzerTM Maxi 6000 Dialysis Kit (Sigma, cat. No. PURX60100-1KT) against dialysis buffer (20 mM Tris ...
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bioRxiv - Plant Biology 2020Quote: ... 1 ml of transfected protoplasts were incubated for 12 h for protein expression in the presence of 1 μM DSF (Sigma, USA), and were treated with or without 1 μM flg22 (MoonBiochem ...
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bioRxiv - Cancer Biology 2021Quote: ... and nascent puromycylated proteins was detected by Western blot analysis as above using a mouse anti-puromycin antibody 12D10 (1:3000)(MABE343, Sigma-Aldrich).
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bioRxiv - Cell Biology 2019Quote: ... Protein translation was analyzed on the WES automated western blotting system (ProteinSimple) using anti-Puromycin antibody (1:50; Sigma-Aldrich, MABE343).
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bioRxiv - Cell Biology 2020Quote: Lamin A proteins were detected by probing with mouse anti-Myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
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bioRxiv - Molecular Biology 2020Quote: HPG labeled proteins in supernatant and pellet fractions (∼1 mg) were click-conjugated with 50 µM azide-PEG3-biotin (Sigma-Aldrich) by a Click-iT Cell Reaction Buffer Kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... immunofluorescence staining was performed using overnight incubation with polyclonal anti-Prosurfactant Protein C (ProSP-C) (Merck/Millipore/Sigma Aldrich, 1:500) followed by staining with polyclonal secondary antibody Goat anti-rabbit Alexa fluor 488 (Invitrogen,1:500) ...
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bioRxiv - Developmental Biology 2022Quote: ... immunofluorescence staining was performed using overnight incubation with polyclonal anti-Prosurfactant Protein C (ProSP-C) (Merck/Millipore/Sigma Aldrich, 1:500) followed by staining with polyclonal secondary antibody Goat anti-rabbit Alexa fluor 488 (Invitrogen,1:500) ...
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bioRxiv - Developmental Biology 2019Quote: ... 1 nl of 12.5 ng/µl of gRNA and 1 nl of Cas9 protein (300 ng/ul) (Jinek et al., 2012) along with phenol red (Sigma Aldrich) were co-injected at one-cell stage ...
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bioRxiv - Biophysics 2020Quote: ... and trypsinized at an enzyme to protein mole ratio of 1:50 at 37 °C in a 3M spin-filter device (Millipore Inc.,). Peptides were then desalted using PepClean spin filters (Pierce Inc. ...
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bioRxiv - Neuroscience 2019Quote: ... The protein-DNA complexes of the frontal cortical tissue were crosslinked through incubation with 1% formaldehyde (Sigma-Aldrich, St. Louis, MO) on a rotator for 12 minutes ...
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bioRxiv - Genomics 2020Quote: Cells growing in log phase were harvested for protein extraction using the protein extraction buffer (50 mM Tris–HCl, 150 mM NaCl, 1% Triton-X, 10% glycerol, 5 mM EDTA; all Sigma-Aldrich) and 0.5 μl protease inhibitor mix (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2021Quote: purified PEGylated ferritin cages were used to synthesize a magnetite core inside the cage using 1 mg/ml protein concentration diluted in 25 ml of 100 mM NaCl (Sigma-Aldrich). Solution of 30 mM ammonium iron (II ...
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... 0.2mg of PNS were incubated for 4 hours at 4°C with 5 μl of protein A magnetic beads (Pierce Thermoscientific) pre-incubated (1 hour at RT) with 2 μg of anti-FLAG antibody (Sigma, F7425). Immune complexes were finally washed 3 times with lysis buffer and eluted by incubating the beads (5’ at 70°C ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h at RT with anti-microtubule associated protein 2 (MAP2) mouse monoclonal antibody (diluted 1:200 in PBS/gelatin; Sigma-Aldrich) and with anti-Tau rabbit polyclonal antibody (diluted 1:500 in PBS/gelatin ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Microbiology 2020Quote: ... FLAG- and HA-tagged proteins were detected by Western blotting using a 1:1000 dilution of primary anti-FLAG rabbit polyclonal antibody (Sigma, F7425) or anti-HA rabbit polyclonal antibody (Invitrogen ...
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bioRxiv - Systems Biology 2020Quote: ... Bait proteins were probed using mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody, Sigma-Aldrich, F3165; used at 1:2500) in blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were adjusted to 1-2 mg total protein in 1 mL and incubated overnight at 4°C with 25 µL of packed Monoclonal Anti-HA Agarose (Sigma-Aldrich). Following incubation ...
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bioRxiv - Physiology 2021Quote: ... Trypsin was added at a ratio of 1:50 trypsin to protein (trypsin from porcine pancreas, Sigma Aldrich, catalog number T6567). Samples were incubated at 37°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... 80 µg protein were loaded into a 10 % SDS gel and stained with anti-Tau (clone Tau46, T9450, mouse, 1:1000, Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... lysates were incubated with 10 ul bed volume per 1 mg protein of anti-myc 9E10-conjugated agarose beads (Sigma Aldrich). Beads were then centrifuged and washed 3X with lysis buffer supplemented with 300mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... The eluted protein was concentrated to 2-3 mg ml-1 using an Amicon ultracel-100 centrifugal filter unit (EMD Millipore) for amphipol exchange or nanodisc reconstitution ...
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bioRxiv - Biophysics 2020Quote: ... The native samples were then concentrated to a final protein concentration of 0.23 mg mL−1 using 3K MWC Amicon® Ultra-15 Centrifugal Filter Units (Millipore Sigma) (Amicon Filter) ...
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bioRxiv - Molecular Biology 2022Quote: ... Each recombinant protein was eluted twice in 250 µl of EB containing 1 mM DTT and 250 µg/ml 3xFLAG peptide (Sigma-Aldrich). Eluates were pooled and concentrated using a Vivaspin 500 (GE Healthcare).
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bioRxiv - Bioengineering 2022Quote: ... The residual proteins bound to exDNA were digested by 2-h incubation with 0.85 g L−1 proteinase K (Sigma-Aldrich, UK) at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein loading was measured using a monoclonal β-actin antibody directly conjugated to horseradish peroxidase (1:20,000; AC-15, Sigma-Aldrich), nucleolin (1:5000 ...
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bioRxiv - Microbiology 2022Quote: ... Epitope-tagged 3xFLAG proteins were detected using primary antibody anti-FLAG M2 from mouse (diluted 1:4,000, catalogue number F3165; Sigma Aldrich, Germany) and Alexa Fluor 680 fluorescent dye-labeled secondary anti-mouse antibody from goat (diluted 1:10,000 ...
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bioRxiv - Microbiology 2020Quote: ... HTF-tagged LsaA and FLAG3-tagged VgaALC proteins were detected using anti-Flag M2 primary (Sigma-Aldrich, F1804; 1:10,000 dilution) antibodies combined with anti-mouse-HRP secondary (Rockland ...
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bioRxiv - Biochemistry 2022Quote: ... Each recombinant protein was eluted twice in 250 μL EB containing 1 mM DTT and 250 μg/mL 3×FLAG peptide (Sigma-Aldrich). Eluates were pooled and concentrated using a Vivaspin 500 (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... The fractions containing protein were pooled and concentrated to 1 ml final volume using an Amicon Ultra-15 30 kDa concentrator (Millipore, Z717185). This was subjected to gel filtration purification on a Superdex 75 Increase 10/300GL column equilibrated in Equilibration Buffer [50 mM Tris/HCl pH7.5 ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated overnight at 4 °C with mouse anti-SMI31/32 (to label neurofilament proteins; Millipore NE1022/NE1023; 1:1000) and chicken anti-myelin basic protein (to label myelin ...
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bioRxiv - Pathology 2020Quote: ... and specific proteins were detected by immunostaining with the following antibodies: Cy3-coupled mouse anti-ACTA2 (C6198-100UL, 1:500, Sigma-Aldrich) or biotinylated mouse anti-ACTA2 (MS-113-B0 ...
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bioRxiv - Physiology 2020Quote: ... Trypsin was added at a ratio of 1:50 trypsin to protein (trypsin from porcine pancreas, Sigma Aldrich, catalog number T6567). Samples were incubated at 37°C overnight ...
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bioRxiv - Synthetic Biology 2020Quote: ... The loading solution consisted of 15 µL of the protein sample mixed with 1 µL DTT and 15 µL Laemmli 2× Concentrate Loading Buffer (Sigma-Aldrich), and denatured at 95 °C for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... The ΔNt17-Httex1 (6-42Q) proteins were dissolved in 10 mM PBS and 1 mM Tris (2-carboxyethyl)phosphine (TCEP, Sigma–Aldrich) to keep the N-terminal cysteine reduced.
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bioRxiv - Molecular Biology 2021Quote: ... Anti-flavivirus group antigen antibody that probes ZIKV E protein and anti-SARS-CoV-1/2 NP 1C7C7 antibody was purchased from Sigma-Aldrich. Anti-influenza A virus nucleoprotein (NP ...
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bioRxiv - Developmental Biology 2019Quote: Protein samples were obtained by replacing cell culture medium with 1% sodium dodecyl sulfate containing 1X protease inhibitor cocktail (Sigma P8340). Separation was performed in 4-12% Bis-Tris NuPAGE gels (Invitrogen ...
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bioRxiv - Physiology 2021Quote: ... Trypsin was added at a ratio of 1:50 trypsin to protein (trypsin from porcine pancreas, Sigma Aldrich, catalog number T6567). Samples were incubated at 37°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... Unbound protein was washed off in PBST (3x 5 min) and bound protein was detected using HRP-conjugated anti-His antibody (1:2000 in 5% milk PBST; Novagen #71841). After 1 hour incubation at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... precleared cell extracts were incubated with the indicated antibody for 4 hours at 4°C with rotation followed by 1 hour of pull-down by 1:1 protein A/G agarose beads (GE, 17061801, 17127901) or FLAG/Myc beads (FLAG resin: Sigma, A2220; Myc resin: Sigma, E6654). Immunoprecipitates were washed with lysis buffer three times before electrophoresis.
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bioRxiv - Biochemistry 2021Quote: ... Expression of the NK1R-miniGs399fusion protein was induced and enhanced 18 hours after transfection with addition of 1 μg/mL doxycycline hyclate (Sigma Aldrich), 10 mM sodium butyrate (Sigma Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... The bacterial culture was then induced to produce the recombinant protein by the addition of 0.5 M isopropyl β-d-1-thiogalactopyranoside (IPTG) (Sigma-Aldrich) and was allowed to grow for a further 3 h ...
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bioRxiv - Genetics 2022Quote: ... TAP-tagged Rrp45 protein was detected with peroxidase anti-peroxidase (PAP) soluble complex antibody produced in rabbit (1:5000, Sigma-Aldrich). The 3-phosphoglycerate kinase (Pgk1 ...
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bioRxiv - Plant Biology 2022Quote: ... The membranes were washed with Tris buffered saline containing 0.1% Tween-20 and probed again for protein loading control using a 1:10000 dilution of mouse monoclonal anti-actin (plant) primary antibody (Sigma, A0480) and a 1:3000 dilution of goat anti-mouse IgG HRP conjugate (Biorad).
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bioRxiv - Microbiology 2022Quote: ... diluted 1:5000 to detect RVFV and anti-Gc protein antibody (4C12A1) diluted 1:5000 to detect LACV with FFA staining buffer (1X PBS, 1mg/ml saponin (Sigma: 47036)) as a primary detection antibody overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed twice with cold phosphate-buffer saline (PBS) and total proteins were precipitated in 5% trichloroacetic acid (TCA) with 1 mM Methionine (Met) (Sigma-Aldrich) for 15 minutes on ice ...