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Citations for Millipore Sigma :
5551 - 5600 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and transferred at 110mV for one hour to PVDF membranes (Millipore). Membranes were blocked with 5% milk for 1 hour and incubated at 4°C overnight with one or more primary antibodies in 2% bovine serum albumin ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were developed with a chemiluminescent HRP substrate (Millipore Sigma, WBKLS) and imaged using a ChemiDoc MP (BioRad) ...
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bioRxiv - Genetics 2023Quote: ... Membranes were blocked in PBS with 3% milk powder (Sigma, 70166) and 0.1% Tween-20 (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... electrophoresed and transferred to a polyvinylidene difluoride membrane (Millipore, Schwalbach, Germany). The membrane was blocked with 5% BSA in Tris-buffered saline with 0.1% Tween 20 ...
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bioRxiv - Microbiology 2024Quote: ... which was overlaid with two Breathe Easy sealing membranes (Sigma Aldrich). The microplate was shaken at 250 RPM at 37°C for 1 hour whereupon OD600 reached ∼ 0.1 ...
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bioRxiv - Microbiology 2024Quote: ... which was subsequently overlaid with two Breathe Easy sealing membranes (Sigma). Cultures were shaken for 24 hours at 250 RPM and at 37°C whereupon the Breathe Easy sealing membranes were removed and cell density was measured using OD600 in a Victor NIVO microplate reader (Perkin Elmer) ...
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bioRxiv - Microbiology 2024Quote: ... The plate was overlaid with two Breathe Easy sealing membranes (Sigma) and shaken at 37°C and 250RPM in a darkened incubator ...
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bioRxiv - Microbiology 2024Quote: Infectious media was filtered using a 0.45 μM membrane (Millipore SE1M003M00), aliquoted into 1 mL portions ...
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bioRxiv - Microbiology 2024Quote: ... resolved samples were electroblotted onto polyvinyl difluoride (PVDF) membranes (Millipore, USA) and were detected by anti-GroEL2 antibody (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... Plates were sealed with a Breathe-Easy gas permeable membrane (Sigma) and statically cultured in the BioTek plate reader at 37° C for as long as needed (usually 72 h ...
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bioRxiv - Immunology 2024Quote: ... Membranes were developed with Luminata Crescendo Western HRP Substrate (Millipore, WBWR0500). Primary antibody information can be found in Table 1.
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bioRxiv - Bioengineering 2024Quote: ... The buffer was filtered on a 0.22 µm MCE membrane (Millipore) before use ...
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bioRxiv - Cell Biology 2024Quote: ... The media was filtered using 0.45um vacuum filters (Membrane filter Millipore_654). Stock animals were fed twice per week with beef liver paste and the experimental animals were starved for 7 days prior to the experiment ...
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bioRxiv - Cell Biology 2024Quote: ... were filter washed using a 0.45µM pore size nitrocellulose membrane (Millipore), washed twice with SD + heavy Lysine and resuspended in SD + heavy Lysine ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were transferred onto poly-vinylidene difluoride (PVDF) membranes (Millipore, IPFL00010) for immunoblotting and blocked using 5% non-fat dry milk dissolved in tris buffered saline with 0.1% Tween-20 (Fisher ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was then filtered through a 0.45 μm membrane (Millipore), and transferred to a column ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were transferred to a PVDF Immobilon-P membrane (Millipore #IPVH00010) at 100 V for 1,5 hours at 4°C in ice-cold Tris/Glycine buffer containing 20% methanol ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were exposed to Immobilon Crescendo Western HRP substrate (Millipore Sigma) for 30-60 s and chemiluminescent images were acquired by the ChemiDoc System ...
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bioRxiv - Neuroscience 2024Quote: ... Signals from membranes were detected with ECL chemiluminescence (Immoblion Western, Millipore) using a CCD-digital camera system (Amersham Imager 680 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated in monoclonal Reelin antibody (Millipore Cat# MAB5364, RRID:AB_2179313) overnight at 4 degrees before incubation with secondary for 2 hours (#926-68072 ...
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bioRxiv - Neuroscience 2024Quote: ... The transfer was performed using a PVDF membrane (cat. # IPFL00010; Millipore) and a 1x transfer buffer diluted from a 10x stock (cat ...
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bioRxiv - Microbiology 2024Quote: ... membranes were incubated with monoclonal primary anti-6xHis antibody (Sigma-Aldrich) in 1% milk TBST at 1:3,000 ...
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bioRxiv - Microbiology 2024Quote: ... sealed with a Breathe-Easy® sealing membrane (Z380059, Sigma-Aldrich). Every 48 hours ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was washed and developed with luminol/peroxide (Millipore, WBKLS0500) and visualized with an iBright CL-750 imager (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... the tissue was mounted on a black nitrocellulose filter membrane (Millipore) with the ganglion cell side up ...
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bioRxiv - Molecular Biology 2024Quote: ... or BCIP/NBT-Blue Liquid Substrate Systems for Membranes (Sigma B3804) in accordance with manufacturer’s protocols.
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bioRxiv - Synthetic Biology 2024Quote: ... dialyzed the product using 0.025 µm MCE Membrane (MF-Millipore VSWP02500), transformed it into E ...
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bioRxiv - Plant Biology 2024Quote: ... Membranes were blocked in PBS-T (tablets 524650-1EA, Sigma-Aldrich) containing 0.05% Tween-20 and 5% skim milk for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... sealed with a Breathe-Easy® sealing membrane (Z380059, Sigma-Aldrich). The pH was determined using non-bleeding MColorpHast pH indicator strips (Merck Millipore) ...
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bioRxiv - Immunology 2020Quote: ... bead-based kit (Milliplex 41-plex human cytokine panel 1, Millipore, MA, USA) on the FLEXMAP 3D system (Luminex Corporation ...
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bioRxiv - Cancer Biology 2019Quote: ... 5-10 μg protein lysates were cultured and proteasome activity measured using the 20S Proteasome Activity Assay kit (APT280, Millipore, Billerica, MA, USA) per manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... The samples were then clarified by centrifugation and the protein content of each supernatant was determined using the Quanti-Pro bicinchoninic acid (BCA) assay kit (Sigma-Aldrich, MO, USA). Equal protein amounts (100 μg ...
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bioRxiv - Immunology 2023Quote: Cells from day 5 bone marrow cultures from tet-LIN28B or littermate control mice were used for lysis and RIP using Magna RIP RNA-Binding Protein Immunoprecipitation kit (Millipore, cat#17-700) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated at 4 °C overnight in the same solution with the primary rabbit anti-OMP (olfactory marker protein; 1:500, O7889 from Sigma). Immunofluorescence was achieved by reaction with the secondary antibody donkey anti-rabbit-488 (A21206 from Molecular Probes ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were stained overnight at 4°C with primary antibody (Mouse anti-γH2AX antibody; Millipore Sigma #05-636, 1:300) diluted in blocking solution ...
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bioRxiv - Cell Biology 2022Quote: ... Immunohistochemistry was used to detect protein expression using the following antibodies on paraffin sections: SftpC (rabbit, Millipore, AB37086, 1:250), and Ki67 (eBioscience ...
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bioRxiv - Genomics 2019Quote: ... 0.04% (v/v) Tween-20) and FLAG-tagged protein were incubated for 1 hr with Anti-FLAG M2 Affinity Gel (Sigma) while rotating ...
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bioRxiv - Molecular Biology 2019Quote: ... 200 µg protein were incubated with DNase I (7,5 U) and 1 µg anti-Flag resin (cat# F3165, Sigma-Aldrich) at 12 rpm overnight at 4°C ...
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bioRxiv - Physiology 2019Quote: ... Hydrolyzed protein diet was prepared by incubating 5 mL intact protein diet with 43.7 mg trypsin (Trypsin Powder, Porcine 1:250, 85450C, Sigma Aldrich) at 37°C for 20 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein digestion was performed overnight at 37 °Cwith Lys-C (Wako) at an enzyme/protein ratio 1:75 and trypsin (Sigma) at an enzyme/protein ration of 1:50 ...
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bioRxiv - Microbiology 2020Quote: ... and equal amounts of protein were analyzed using the following antibodies: anti-FLAG M2 (Sigma, cat#F3165, RRID:AB_259529; 1:7,500), anti-IE1 (clone 1B12 (Zhu et al. ...
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bioRxiv - Biochemistry 2021Quote: ... Protein extracts were incubated overnight with 1 μg of anti-PDGFRβ (D-6) antibody and immunocomplexes recovered using 25 µL Protein G Sepharose beads (Sigma) for 1 h 30 min in the wheel at 4°C ...
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bioRxiv - Biochemistry 2020Quote: A protein/Sypro orange mix containing 5 μM protein and a 1:5,000 dilution of dye in DMSO (as supplied by Sigma Aldrich) was prepared just before plate setup in 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Residual methylation reagents were neutralised by addition of 1M Tris-HCl pH 7.5 to a final concentration of 50 mM Tris-HCl and the protein complex was concentrated to 2 mg mL−1 using 30 kDa cut-off concentrators (Millipore) before injection into a S75 16/600 column (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... After incubation for 90 min at RT the microplates were washed 4X (PBS-Tween 20) and 25 μl of protein LA-HRP diluted 1:3000 (Sigma) was added and the microplates were then incubated for 1h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... the codon optimized gene encoding for rPMDs was subcloned into PET28a (+) and protein expression in E.coli cells was induced with 1 mM isopropyl-β-D-thiogalactoside (IPTG) (Sigma). The his-tagged proteins were purified using Ni-NTA chromatography and the concentration was determined using the BCA estimation kit (Pierce).
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bioRxiv - Biophysics 2020Quote: Wild-type or Y473E mutant human PPARγ LBD (UniProt P37231; residues 203–477, isoform 1 numbering) protein was expressed from a pET46 Ek/LIC vector (Novagen) as a Tobacco Etch Virus (TEV)-cleavable N-terminal Hexa(6x)His-tagged fusion protein in Escherichia coli BL21(DE3 ...
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bioRxiv - Cell Biology 2021Quote: ... and the dried protein pellet resuspended in digestion buffer (100 mM Tris-HCl pH 8.5, 1% sodium deoxycholate (Sigma-Aldrich), 5 mM TCEP ...
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bioRxiv - Biochemistry 2022Quote: ... The beads were washed three times with 0.5 ml of FLAG wash buffer and the bound protein was eluted with 1X SDS-PAGE loading buffer supplemented with 100 µg·ml-1 3X FLAG peptide (Sigma-Aldrich) on ice ...
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bioRxiv - Biochemistry 2022Quote: ... PKG Iβ (71-686) protein was concentrated to 20 mg ml-1 by using 30 kDa cutoff Amicon Ultra (Millipore) and initial crystal screening was performed by Mosquito Crystal robot (TTP Labtech ...